West Nile Virus (WNV) TaqMan RT-PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz West Nile Virus (WNV) TaqMan RT-PCR Detection Kit Dx Price USD$349.00 SKU DxTM44200 Format RT-PCR Size 24 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for WNV Available in TaqMan format for analysis CE-IVDD marked in accordance with the European Commission Directive 98/79/EC. Research version available West Nile Virus (WNV) belongs to the RNA virus family of Flaviviridae. It is known to spread primarily through arthropod vectors such as mosquitoes. The virus infects mainly birds but is also reported to infect humans as well as pets such as cats and dogs. In humans, the majority of the infections caused by WNV are asymptomatic. However, some individuals (the majority of the confirmed cases) could enter a second, febrile stage with flu-like symptoms – commonly known as West Nile Fever. In a more serious stage, the disease becomes neuroinvasive, causing meningitis or encephalitis. Such severe conditions could lead to mortality. As the symptoms of WNV are very similar to other common diseases but can be fatal at a severe stage, it is important to distinguish the virus early in the diagnosis, particularly at the molecular level. TaqMan PCR Kit Click to expand options WNV TaqMan RT-PCR Kit, 24 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Storage Conditions The WNV TaqMan RT-PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. DxTM44200 (24 rxns) MDx TaqMan 2X RT-PCR Master Mix Dx 550 µL WNV Primer & Probe Mix Dx 2 x 70 µL WNV Positive Control Dx - 200,000 copies/μL 50 µL Nuclease-Free Water 2 x 1.25 mL Product Insert 1 Documentation PROTOCOLS (DxTM44200) WNV TaqMan RT-PCR Kit Dx - Protocol (24 rxns) SAFETY DATA SHEETS (DxTM44200) WNV TaqMan RT-PCR Kit Dx - Safety Data Sheet Citations Powered by Bioz See more details on Bioz Related Products Plasma/Serum RNA Purification Kit
West Nile Virus (WNV) TaqMan RT-PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz West Nile Virus (WNV) TaqMan RT-PCR Detection Kit Dx Price USD$349.00 SKU DxTM44200 Format RT-PCR Size 24 Reactions Quantity