• Detection kits for WNV
  • Available in 2 formats for analysis:
 

ItemsCat. #Size
WNV TaqMan RT-PCR Kit TM44200 24 rxns
WNV TaqMan Probe/Primer and Control Set TM44210 100 rxns
WNV End-Point RT-PCR Kit EP44200 24 rxns
WNV Primer and Control Set EP44210 100 rxns

WNV Detection Kits

West Nile Virus (WNV) belongs to the RNA virus family of Flaviviridae. It is known to spread primarily through arthropod vectors such as mosquitoes. The virus infects mainly birds but is also reported to infect humans as well as pets such as cats and dogs. In humans, the majority of the infections caused by WNV are asymptomatic. However, some individuals (the majority of the confirmed cases) could enter a second, febrile stage with flu-like symptoms – commonly known as West Nile Fever. In a more serious stage, the disease becomes neuroinvasive, causing meningitis or encephalitis. Such severe conditions could lead to mortality. As the symptoms of WNV are very similar to other common diseases but can be fatal at a severe stage, it is important to distinguish the virus early in the diagnosis, particularly at the molecular level.

There are 2 kits available for the detection of WNV:

WNV TaqMan PCR Kit
  • PCR control to monitor for PCR inhibition and validate the quality
  • Master Mix for the target and PCR control detection
  • Primer and Probe mix
  • Positive control and a negative control to confirm the integrity of the kit reagents
WNV End-Point PCR Kit
  • Master Mix and primers for the specific amplification of a 356 nucleotide region of the WNV genome
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate analysis of the results

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.


Kit Components
Component TaqMan Probe
Cat. TM44200
(24 rxns)
End-Point
Cat. EP44200
(24 rxns)
MDx 2X RT-PCR Master Mix - 350 µL
MDx TaqMan 2X RT-PCR Master Mix 350 µL -
WNV Primer Mix 70 µL 70 µL
WNV Positive Control 50 µL 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Loading Dye - 100 µL
DNA Ladder - 100 µL
Product Insert 1 1
Component TaqMan
Probe/Primer
and Control Set
Cat. TM44210
(100 rxns)
Primer
and Control Set
Cat. EP44210
(100 rxns)
WNV Primer Mix 3 x 70 µL 3 x 70 µL
WNV Positive Control 3 x 50 µL 3 x 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Product Insert 1 1