XMRV Detection Kits (Cat. TM34700, TM34710, EP34700)

Detection kits for the XMRV
Available in 2 formats for analysis:

TaqMan Probe
Cat. TM34700 End-Point PCR
Cat. EP34700

Items	Cat. #	Size
XMRV TaqMan RT-PCR Kit	TM34700	24 rxns
XMRV TaqMan Probe/Primer and Control Set	TM34710	100 rxns
XMRV End-Point RT-PCR Kit	EP34700	24 rxns
XMRV Primer and Control Set	EP34710	100 rxns

XMRV Detection Kits

Xenotropic murine leukemia virus-related virus (XMRV) is a gammaretrovirus. The virus was first described in 2006 and has since been isolated from human biological samples. XMRV belongs to the family Retroviridae and the genus gammaretrovirus. It has a single-stranded RNA genome that replicates through a DNA intermediate. The virus gets its name due to its close relationship with the murine leukemia viruses (MuLVs). The viral genome is approximately 8100 nucleotides in length and is 95% identical with several endogenous retroviruses of mice. While gammaretroviruses have well-characterized oncogenic effects in animals, they have not been shown to cause human cancers. However, XMRV was recently discovered in human prostate cancers and is the first gammaretrovirus known to infect humans. In addition to prostate cancer, a possible association with chronic fatigue syndrome has been reported, however it has yet to be established whether XMRV is a cause of this disease.

The causal role of XMRV in cancer has yet to be established and the virus does not appear to be capable of transforming cells directly. In prostate cancer, XMRV protein has been found in tumour-associated but nonmalignant stromal cells, but not in the actual prostate cancer cells. This raises the possibility that the virus may support tumorigenesis. In other studies, XMRV proteins and nucleic acids were found in malignant cells.

There are 2 kits available for the detection of XMRV:

XMRV TaqMan RT-PCR Kit

PCR control to monitor for PCR inhibition and validate the quality
Master Mix for the target and PCR control detection
Primer and Probe mix
Positive control and a negative control to confirm the integrity of the kit reagents

XMRV End-Point RT-PCR Kit

Master Mix and primers for the specific amplification of a region of the XMRV genome
Positive control and a negative control to confirm the integrity of the kit reagents
Loading dye and a DNA ladder to facilitate analysis of the results

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.

Protocols:

TM34700 - Protocol (24 rxns)

EP34700 - Protocol (24 rxns)

Safety Data Sheets:

TM34700 - Safety Data Sheet

EP34700 - Safety Data Sheet

Items	Cat. #	Size
XMRV TaqMan RT-PCR Kit	TM34700	24 rxns
XMRV TaqMan Probe/Primer and Control Set	TM34710	100 rxns
XMRV End-Point RT-PCR Kit	EP34700	24 rxns
XMRV Primer and Control Set	EP34710	100 rxns

XMRV Detection Kits

Xenotropic murine leukemia virus-related virus (XMRV) is a gammaretrovirus. The virus was first described in 2006 and has since been isolated from human biological samples. XMRV belongs to the family Retroviridae and the genus gammaretrovirus. It has a single-stranded RNA genome that replicates through a DNA intermediate. The virus gets its name due to its close relationship with the murine leukemia viruses (MuLVs). The viral genome is approximately 8100 nucleotides in length and is 95% identical with several endogenous retroviruses of mice. While gammaretroviruses have well-characterized oncogenic effects in animals, they have not been shown to cause human cancers. However, XMRV was recently discovered in human prostate cancers and is the first gammaretrovirus known to infect humans. In addition to prostate cancer, a possible association with chronic fatigue syndrome has been reported, however it has yet to be established whether XMRV is a cause of this disease.

The causal role of XMRV in cancer has yet to be established and the virus does not appear to be capable of transforming cells directly. In prostate cancer, XMRV protein has been found in tumour-associated but nonmalignant stromal cells, but not in the actual prostate cancer cells. This raises the possibility that the virus may support tumorigenesis. In other studies, XMRV proteins and nucleic acids were found in malignant cells.

There are 2 kits available for the detection of XMRV:

XMRV TaqMan RT-PCR Kit

PCR control to monitor for PCR inhibition and validate the quality
Master Mix for the target and PCR control detection
Primer and Probe mix
Positive control and a negative control to confirm the integrity of the kit reagents

XMRV End-Point RT-PCR Kit

Master Mix and primers for the specific amplification of a region of the XMRV genome
Positive control and a negative control to confirm the integrity of the kit reagents
Loading dye and a DNA ladder to facilitate analysis of the results

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.