Exosomal RNA Isolation Kit
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![Exosomal RNA Isolation Kit](/sites/default/files/styles/norproduct_imagekit/public/images/product_images/58000-1.jpg?itok=AVuNZYmI)
![Exosomal RNA Isolation Kit Components](/sites/default/files/styles/norproduct_imagekit/public/images/product_images/58000-4.jpg?itok=szLl0EWs)
For research use only and NOT intended for in vitro diagnostics.
This kit is for use with Norgen's exosome purification technology only. View more
Aperçu du produit
This kit provides a fast, reliable and convenient method to isolate and concentrate exosomal RNA from previously purified exosomes. It allows for the isolation of RNA from intact extracellular vesicles (EVs) purified from different urine or plasma/serum sample volumes. The purification is based on Norgen’s proprietary resin.
The Exosomal RNA Isolation Kit is designed to isolate all sizes of RNA, including microRNA. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Détails
Supporting Data
Figure 1. Isolation of RNA from exosomes purified from different urine volumes. Norgen's Exosomal RNA Isolation Kit (Cat# 58000) was used to isolate RNA from exosomes isolated from different urine volumes purified using Norgen's Urine Exosome Purification Kits (Cat# 57700, 57800 and 57900). 2 μL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated urinary exosomal miR-30a. (A) The average Ct value for urinary exosomal miR-30a is linearly decreasing with increasing the sample input volume. B) The relative amount of the urinary exosomal miR-30a shows excellent linearity with a percentage of recovery of more than 90%.
Figure 2. Isolation of RNA from exosomes purified from different plasma volumes. Norgen's Exosomal RNA Isolation Kit (Cat# 58000) was used to isolate RNA from exosomes isolated from different plasma volumes purified using Norgen's Plasma/Serum Exosome Purification Kits (Cat# 57400, 57500 and 57600). 2 μL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma exosomal miR-26a. (A) The average Ct value for plasma exosomal miR-26a is linearly decreasing with increasing the sample input volume. B) The relative amount of the plasma exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%.
Kit Specifications | |
Sample type | Exosomes purified using Norgen’s Purification Kits and most other methods |
Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
Elution Volume | 50-100 μL |
Time to Complete 10 Purifications | 35-40 minutes |
Average Yields* | Variable depending on specimen |
*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Component | Cat. 58000 (50 preps) |
---|---|
Lysis Buffer A | 20 mL |
Lysis Additive B | 2 mL |
Wash Solution A | 18 mL |
Elution Solution A | 6 mL |
Mini Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |