Human Papillomavirus (HPV) (High and Low Risk) TaqMan PCR Detection Kits

Supporting Data

TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.

End-Point PCR Kit - Figure 4. A representative 1X TAE, 1.4 % agarose gel showing the amplification of HPV (High and Low Risk) at different concentrations. The size of the HPV (High and Low Risk) target amplicon corresponds to the 280 bp band represented by the provided DNA Marker (M).

Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM31550 (100 preps)	Cat. TM31510 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	-
HPV (High and Low Risk) Primer & Probe Mix	280 μL	280 μL
HPV (High and Low Risk) Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Documentation

PROTOCOLS

(TM31550) HPV (High and Low Risk) TaqMan PCR Kit - Protocol (100 rxns)

(TM31510) HPV High and Low Risk TaqMan Probe/Primer and Control Set - Protocol (100 rxns)

Human Papillomavirus (HPV) (High and Low Risk) TaqMan PCR Detection Kits