FFPE RNA/DNA Purification Plus Kit Dx Sequential isolation and purification of total RNA and genomic DNA from FFPE tissue samplesResearch version available here Powered by Bioz FFPE RNA/DNA Purification Plus Kit Dx Price USD$655.00 SKU Dx54300 Format Plus Size 50 Preps Quantity Overview Details Documentation Citations Overview Fast and easy processing using rapid spin-column format High yields and quality of nucleic acids Separate fractionation of RNA and DNA Isolate total RNA, from large rRNA down to microRNA (miRNA) No phenol or chloroform extractions Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s FFPE RNA/DNA Purification Plus Kit Dx provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples for subsequent in vitro diagnostic use. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE RNA/DNA Purification Plus Kit Dx provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of nucleic acids followed by signal detection or amplification. Any diagnostic results generated using the RNA and DNA isolated with Norgen’s FFPE RNA/DNA Purification Plus Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings. To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s FFPE RNA/DNA Purification Plus Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s FFPE RNA/DNA Purification Plus Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay. Details Supporting Data Previous Figure 1. Superior Recovery of High Quality RNA and DNA from FFPE Spleen Tissues. Norgen's FFPE RNA/DNA Purification Plus Kit Dx isolates FFPE RNA and DNA that exceeds the yield of competitors. Total RNA and DNA was isolated from equal amount of hamster FFPE spleen sections (20 micron thickness) using Norgen's FFPE RNA /DNA Purification Plus Kit Dx and a leading competitor’s kits. Triplicate isolations were performed for each product. The top graphs demonstrate the mean yield of RNA (Panel A) and DNA (Panel B) according to NanoDrop measurement. The bottom graphs showed the mean 260:280 ratio and 260:230 ratio of RNA (Panel C) and DNA (Panel D) according to NanoDrop measurement. Norgen's kit consistently purified total RNA and DNA with a higher yield and higher quality than for those obtained using the market competitor's kits. Figure 2. Recovery of High Quality DNA and True Total RNA including microRNA from Hamster Spleen. Genomic DNA isolated using Norgen's FFPE RNA/DNA Purification Plus Kit Dx is of high quality with effective qPCR amplification. Panel A showed that in a qPCR using primers against the 5S rRNA gene using equal amount of FFPE DNA as template, the DNA isolated with Norgen's kit showed better amplification (lower Ct value). Moreover, Norgen's FFPE RNA/DNA Purification Plus Kit isolates true total RNA including microRNA. With the same amount of RNA as template in RT-qPCR reactions for transcripts of miR-21 (Panel B for microRNA) and beta-Actin (Panel C for mRNA), RNA isolated by Norgen showed better amplification in beta-Actin and significantly better in miR-21 microRNA. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity (RNA) 35 μg Maximum Column Binding Capacity (gDNA) 10 μg Maximum Column Loading Volume 600 μL Size of RNA Purified All sizes, including small RNA (< 200 nt) Maximum Amount of Starting Material 4 sections <20 μM thick 10 mg of unsectioned block Storage Conditions and Product Stability The DNAse I and Proteinase K should be stored at -20°C upon arrival. All other solutions and kit components should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels. Component Cat. 54300 (50 preps) Digestion Buffer 2 x 20 mL Binding Solution 2 x 20 mL Enzyme Incubation Buffer A 6 mL RNA Wash Solution 24 mL DNA Wash Solution 38 mL RNA Elution Solution 6 mL DNA Elution Buffer 15 mL Proteinase K 2 x 12 mg DNase I 200 μL RNA Purification Micro Columns 50 DNA Purification Micro Columns 50 Collection Tubes 100 Elution Tubes (1.7 mL) 100 Product Insert 1 Documentation PROTOCOLS (Dx54300) FFPE RNA/DNA Purification Plus Kit Dx - Protocol (50 preps) PRODUCT INFORMATION SHEETS FFPE RNA/DNA Purification Plus Kit - Product Information Sheet SAFETY DATA SHEETS (Dx54300) FFPE RNA/DNA Purification Plus Kit Dx - Safety Data Sheet FLYERS Flyer Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Title The use of whole genome methylation scanning to define genes preferentially suppressed in African American Prostate Cancer Journal Cancer Epidemiology, Biomarkers and Prevention. 2017. Authors Farah Rahmatpanah, Kathleen McGuire, Michael Lilly, Michael McClelland and Dan Mercola Title MicroRNA-34c-5p is related to recurrence in laryngeal squamous cell carcinoma Journal The Laryngoscope. 2015. Authors Massimo Re MD, Artan Çeka MD, Corrado Rubini MD, Luigi Ferrante PhD, Antonio Zizzi PhD, Federico M. Gioacchini MD, Michele Tulli MD, Liana Spazzafumo PhD, Stefano Sellari-Franceschini MD, Antonio D. Procopio MD, andFabiola Olivieri PhD Title In-Vivo Fusion of Human Cancer and Hamster Stromal Cells Permanently Transduces and Transcribes Human DNA Journal PLOS ONE. 2014. Authors David M. Goldenberg, Robert J. Rooney, Meiyu Loo, Donglin Liu, Chien-Hsing Chang Title The miRNA-23b/27b/24 Cluster Promotes Breast Cancer Lung Metastasis by Targeting Metastasis-Suppressive Gene Prosaposin. Journal The Journal of Biological Chemistry. 2014. Authors Brian Ell, Qiong Qiu, Yong Wei, Laura Mercatali, Toni Ibrahim, Dino Amadori and Yibin Kang Title Tumor-Induced Osteoclast miRNA Changes as Regulators and Biomarkers of Osteolytic Bone Metastasis. Journal Cancer Cell. 2013. Authors Ell B, Mercatali L, Ibrahim T, Campbell N, Schwarzenbach H, Pantel K, Amadori D, Kang Y. Title Tyrosine kinase receptor status in endometrial stromal sarcoma: an immunohistochemical and genetic-molecular analysis. Journal International Journal of Gynecology Pathology. 2012. Authors Cossu-Rocca P, Contini M, Uras MG, Muroni MR, Pili F, Carru C, Bosincu L, Massarelli G, Nogales FF, De Miglio MR. Title Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections. Journal BMC Research Notes. 2012. Authors Patnaik SK, Kannisto E, Yendamuri S. Title Overexpression of the Lung Cancer-Prognostic miR-146b MicroRNAs Has a Minimal and Negative Effect on the Malignant Phenotype of A549 Lung Cancer Cells. Journal PLoS One. 2011. Authors Patnaik S, Kannisto E, Mallick R, Yendamuri S. Related Services NGS RNA Isolation Related Products FFPE RNA/DNA Purification Plus Kit FFPE RNA Purification 96-Well Kit FFPE DNA Purification Kit RNA/DNA/Protein Purification Plus Kit Leukocyte RNA Purification Kit
FFPE RNA/DNA Purification Plus Kit Dx Sequential isolation and purification of total RNA and genomic DNA from FFPE tissue samplesResearch version available here Powered by Bioz FFPE RNA/DNA Purification Plus Kit Dx Price USD$655.00 SKU Dx54300 Format Plus Size 50 Preps Quantity