FFPE RNA/DNA Purification Plus Kit

Cat. 54300
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FFPE RNA/DNA Purification Plus Kit

Cat. 54300

Sequential isolation and purification of total RNA and genomic DNA from FFPE tissue samples

  • Fast and easy processing using rapid spin-column format
  • High yields and quality of nucleic acids
  • Separate fractionation of RNA and DNA
  • Isolate total RNA, from large rRNA down to microRNA (miRNA) 
  • No phenol or chloroform extractions
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Formerly Cat. 25000

CE-IVDD marked diagnostic version available here

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FFPE RNA/DNA Purification Plus Kit
(Cat. 54300)
50 preps

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

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Kit Specifications

You have selected: Cat. 54300
Kit Specifications
Maximum Binding Capacity
Up to 35 μg RNA
 Up to 10 μg DNA
Maximum Column Loading Volume 
600 μL
Size of RNA Purified

All sizes, including small RNA (< 200 nt) 

Maximum Amount of Starting Material 
4 sections <20 μM thick
10 mg of unsectioned block

 

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year after the date of shipment.

Supporting Data

Click for expanded view

Title Multiregion sequencing of sarcomatoid renal cell carcinoma arising from autosomal dominant polycystic kidney disease
Journal Molecular Genetics & Genomic Medicine. 2022.
Authors Elizabeth Lee, Peiyong Guan, Abner Herbert Lim, Jui Wan Loh, Grace Fangmin Tan, Tracy Loh, Dave Yong Xiang Ng, Jing Yi Lee, Shane Goh, Wei Liu, Cedric Chuan-Young Ng, Bin Tean Teh, Jason Yongsheng Chan
Title Decreased expression of miR-23b is associated with poor survival of endometrial cancer patients
Journal Research Square. 2022.
Authors Paweł K. Włodarski, Klaudia Klick, Tomasz M. Grzywa, Alicja Klinke, Aleksandra Mielniczuk, Jarosław Wejman, Joanna Ostrowska, Agata Gondek
Title Methodological Challenges of Digital PCR Detection of the Histone H3 K27M Somatic Variant in Cerebrospinal Fluid
Journal Pathol Oncology & Research. 2022.
Authors Zaytseva M, Usman N, Salnikova E, Sanakoeva A, Valiakhmetova A, Chervova A, Papusha L, Novichkova G, Druy A
Title The use of whole genome methylation scanning to define genes preferentially suppressed in African American Prostate Cancer
Journal Cancer Epidemiology, Biomarkers and Prevention. 2017.
Authors Farah Rahmatpanah, Kathleen McGuire, Michael Lilly, Michael McClelland and Dan Mercola
Title MicroRNA-34c-5p is related to recurrence in laryngeal squamous cell carcinoma
Journal The Laryngoscope. 2015.
Authors Massimo Re MD, Artan Çeka MD, Corrado Rubini MD, Luigi Ferrante PhD, Antonio Zizzi PhD, Federico M. Gioacchini MD, Michele Tulli MD, Liana Spazzafumo PhD, Stefano Sellari-Franceschini MD, Antonio D. Procopio MD, andFabiola Olivieri PhD
Title In-Vivo Fusion of Human Cancer and Hamster Stromal Cells Permanently Transduces and Transcribes Human DNA
Journal PLOS ONE. 2014.
Authors David M. Goldenberg, Robert J. Rooney, Meiyu Loo, Donglin Liu, Chien-Hsing Chang
Title The miRNA-23b/27b/24 Cluster Promotes Breast Cancer Lung Metastasis by Targeting Metastasis-Suppressive Gene Prosaposin.
Journal The Journal of Biological Chemistry. 2014.
Authors Brian Ell, Qiong Qiu, Yong Wei, Laura Mercatali, Toni Ibrahim, Dino Amadori and Yibin Kang
Title Tumor-Induced Osteoclast miRNA Changes as Regulators and Biomarkers of Osteolytic Bone Metastasis.
Journal Cancer Cell. 2013.
Authors Ell B, Mercatali L, Ibrahim T, Campbell N, Schwarzenbach H, Pantel K, Amadori D, Kang Y.
Title Tyrosine kinase receptor status in endometrial stromal sarcoma: an immunohistochemical and genetic-molecular analysis.
Journal International Journal of Gynecology Pathology. 2012.
Authors Cossu-Rocca P, Contini M, Uras MG, Muroni MR, Pili F, Carru C, Bosincu L, Massarelli G, Nogales FF, De Miglio MR.
Title Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections.
Journal BMC Research Notes. 2012.
Authors Patnaik SK, Kannisto E, Yendamuri S.

FFPE RNA/DNA Purification Plus Kit

Norgen’s FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. The RNA and DNA are sequentially purified into separate fractions using this kit. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE RNA/DNA Purification Plus Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The purified genomic DNA is also of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting and SNP analysis.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 54300
Kit Specifications
Maximum Binding Capacity
Up to 35 μg RNA
 Up to 10 μg DNA
Maximum Column Loading Volume 
600 μL
Size of RNA Purified

All sizes, including small RNA (< 200 nt) 

Maximum Amount of Starting Material 
4 sections <20 μM thick
10 mg of unsectioned block

 

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year after the date of shipment.

Title Multiregion sequencing of sarcomatoid renal cell carcinoma arising from autosomal dominant polycystic kidney disease
Journal Molecular Genetics & Genomic Medicine. 2022.
Authors Elizabeth Lee, Peiyong Guan, Abner Herbert Lim, Jui Wan Loh, Grace Fangmin Tan, Tracy Loh, Dave Yong Xiang Ng, Jing Yi Lee, Shane Goh, Wei Liu, Cedric Chuan-Young Ng, Bin Tean Teh, Jason Yongsheng Chan
Title Decreased expression of miR-23b is associated with poor survival of endometrial cancer patients
Journal Research Square. 2022.
Authors Paweł K. Włodarski, Klaudia Klick, Tomasz M. Grzywa, Alicja Klinke, Aleksandra Mielniczuk, Jarosław Wejman, Joanna Ostrowska, Agata Gondek
Title Methodological Challenges of Digital PCR Detection of the Histone H3 K27M Somatic Variant in Cerebrospinal Fluid
Journal Pathol Oncology & Research. 2022.
Authors Zaytseva M, Usman N, Salnikova E, Sanakoeva A, Valiakhmetova A, Chervova A, Papusha L, Novichkova G, Druy A
Title The use of whole genome methylation scanning to define genes preferentially suppressed in African American Prostate Cancer
Journal Cancer Epidemiology, Biomarkers and Prevention. 2017.
Authors Farah Rahmatpanah, Kathleen McGuire, Michael Lilly, Michael McClelland and Dan Mercola
Title MicroRNA-34c-5p is related to recurrence in laryngeal squamous cell carcinoma
Journal The Laryngoscope. 2015.
Authors Massimo Re MD, Artan Çeka MD, Corrado Rubini MD, Luigi Ferrante PhD, Antonio Zizzi PhD, Federico M. Gioacchini MD, Michele Tulli MD, Liana Spazzafumo PhD, Stefano Sellari-Franceschini MD, Antonio D. Procopio MD, andFabiola Olivieri PhD
Title In-Vivo Fusion of Human Cancer and Hamster Stromal Cells Permanently Transduces and Transcribes Human DNA
Journal PLOS ONE. 2014.
Authors David M. Goldenberg, Robert J. Rooney, Meiyu Loo, Donglin Liu, Chien-Hsing Chang
Title The miRNA-23b/27b/24 Cluster Promotes Breast Cancer Lung Metastasis by Targeting Metastasis-Suppressive Gene Prosaposin.
Journal The Journal of Biological Chemistry. 2014.
Authors Brian Ell, Qiong Qiu, Yong Wei, Laura Mercatali, Toni Ibrahim, Dino Amadori and Yibin Kang
Title Tumor-Induced Osteoclast miRNA Changes as Regulators and Biomarkers of Osteolytic Bone Metastasis.
Journal Cancer Cell. 2013.
Authors Ell B, Mercatali L, Ibrahim T, Campbell N, Schwarzenbach H, Pantel K, Amadori D, Kang Y.
Title Tyrosine kinase receptor status in endometrial stromal sarcoma: an immunohistochemical and genetic-molecular analysis.
Journal International Journal of Gynecology Pathology. 2012.
Authors Cossu-Rocca P, Contini M, Uras MG, Muroni MR, Pili F, Carru C, Bosincu L, Massarelli G, Nogales FF, De Miglio MR.
Title Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections.
Journal BMC Research Notes. 2012.
Authors Patnaik SK, Kannisto E, Yendamuri S.

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