For research use only and NOT intended for in vitro diagnostics.
Sequential isolation and purification of total RNA and genomic DNA from FFPEÂ tissue samples
Formerly Cat. 25000
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FFPE RNA/DNA Purification Plus Kit
Norgen’s FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. The RNA and DNA are sequentially purified into separate fractions using this kit. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE RNA/DNA Purification Plus Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The purified genomic DNA is also of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting and SNP analysis.
Kit Specifications
|
|
Maximum Binding Capacity
|
Up to 35 μg RNA
 Up to 10 μg DNA |
Maximum Column Loading VolumeÂ
|
600 μL
|
Size of RNA Purified
|
All sizes, including small RNA (< 200 nt)Â |
Maximum Amount of Starting Material |
4 sections <20 μM thick
10 mg of unsectioned block |
Â
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
Title | Methodological Challenges of Digital PCR Detection of the Histone H3 K27M Somatic Variant in Cerebrospinal Fluid |
Journal | Pathol Oncology & Research. 2022. |
Authors | Zaytseva M, Usman N, Salnikova E, Sanakoeva A, Valiakhmetova A, Chervova A, Papusha L, Novichkova G, Druy A |
Title | The use of whole genome methylation scanning to define genes preferentially suppressed in African American Prostate Cancer |
Journal | Cancer Epidemiology, Biomarkers and Prevention. 2017. |
Authors | Farah Rahmatpanah, Kathleen McGuire, Michael Lilly, Michael McClelland and Dan Mercola |
Title | MicroRNA-34c-5p is related to recurrence in laryngeal squamous cell carcinoma |
Journal | The Laryngoscope. 2015. |
Authors | Massimo Re MD, Artan Çeka MD, Corrado Rubini MD, Luigi Ferrante PhD, Antonio Zizzi PhD, Federico M. Gioacchini MD, Michele Tulli MD, Liana Spazzafumo PhD, Stefano Sellari-Franceschini MD, Antonio D. Procopio MD, andFabiola Olivieri PhD |
Title | In-Vivo Fusion of Human Cancer and Hamster Stromal Cells Permanently Transduces and Transcribes Human DNA |
Journal | PLOS ONE. 2014. |
Authors | David M. Goldenberg, Robert J. Rooney, Meiyu Loo, Donglin Liu, Chien-Hsing Chang |
Title | The miRNA-23b/27b/24 Cluster Promotes Breast Cancer Lung Metastasis by Targeting Metastasis-Suppressive Gene Prosaposin. |
Journal | The Journal of Biological Chemistry. 2014. |
Authors | Brian Ell, Qiong Qiu, Yong Wei, Laura Mercatali, Toni Ibrahim, Dino Amadori and Yibin Kang |
Title | Tumor-Induced Osteoclast miRNA Changes as Regulators and Biomarkers of Osteolytic Bone Metastasis. |
Journal | Cancer Cell. 2013. |
Authors | Ell B, Mercatali L, Ibrahim T, Campbell N, Schwarzenbach H, Pantel K, Amadori D, Kang Y. |
Title | Tyrosine kinase receptor status in endometrial stromal sarcoma: an immunohistochemical and genetic-molecular analysis. |
Journal | International Journal of Gynecology Pathology. 2012. |
Authors | Cossu-Rocca P, Contini M, Uras MG, Muroni MR, Pili F, Carru C, Bosincu L, Massarelli G, Nogales FF, De Miglio MR. |
Title | Nucleic acids from long-term preserved FFPE tissues are suitable for downstream analyses. |
Journal | Virchows Arch. 2012. |
Authors | Ludyga N, Grünwald B, Azimzadeh O, Englert S, Höfler H, Tapio S, Aubele M. |
Title | Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections. |
Journal | BMC Research Notes. 2012. |
Authors | Patnaik SK, Kannisto E, Yendamuri S. |
Title | Overexpression of the Lung Cancer-Prognostic miR-146b MicroRNAs Has a Minimal and Negative Effect on the Malignant Phenotype of A549 Lung Cancer Cells. |
Journal | PLoS One. 2011. |
Authors | Patnaik S, Kannisto E, Mallick R, Yendamuri S. |
Want a discount?
Create your account to receive 15% off on your first purchase!
FFPE RNA/DNA Purification Plus Kit
Norgen’s FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. The RNA and DNA are sequentially purified into separate fractions using this kit. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE RNA/DNA Purification Plus Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The purified genomic DNA is also of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting and SNP analysis.
Kit Specifications
|
|
Maximum Binding Capacity
|
Up to 35 μg RNA
 Up to 10 μg DNA |
Maximum Column Loading VolumeÂ
|
600 μL
|
Size of RNA Purified
|
All sizes, including small RNA (< 200 nt)Â |
Maximum Amount of Starting Material |
4 sections <20 μM thick
10 mg of unsectioned block |
Â
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
Title | Methodological Challenges of Digital PCR Detection of the Histone H3 K27M Somatic Variant in Cerebrospinal Fluid |
Journal | Pathol Oncology & Research. 2022. |
Authors | Zaytseva M, Usman N, Salnikova E, Sanakoeva A, Valiakhmetova A, Chervova A, Papusha L, Novichkova G, Druy A |
Title | The use of whole genome methylation scanning to define genes preferentially suppressed in African American Prostate Cancer |
Journal | Cancer Epidemiology, Biomarkers and Prevention. 2017. |
Authors | Farah Rahmatpanah, Kathleen McGuire, Michael Lilly, Michael McClelland and Dan Mercola |
Title | MicroRNA-34c-5p is related to recurrence in laryngeal squamous cell carcinoma |
Journal | The Laryngoscope. 2015. |
Authors | Massimo Re MD, Artan Çeka MD, Corrado Rubini MD, Luigi Ferrante PhD, Antonio Zizzi PhD, Federico M. Gioacchini MD, Michele Tulli MD, Liana Spazzafumo PhD, Stefano Sellari-Franceschini MD, Antonio D. Procopio MD, andFabiola Olivieri PhD |
Title | In-Vivo Fusion of Human Cancer and Hamster Stromal Cells Permanently Transduces and Transcribes Human DNA |
Journal | PLOS ONE. 2014. |
Authors | David M. Goldenberg, Robert J. Rooney, Meiyu Loo, Donglin Liu, Chien-Hsing Chang |
Title | The miRNA-23b/27b/24 Cluster Promotes Breast Cancer Lung Metastasis by Targeting Metastasis-Suppressive Gene Prosaposin. |
Journal | The Journal of Biological Chemistry. 2014. |
Authors | Brian Ell, Qiong Qiu, Yong Wei, Laura Mercatali, Toni Ibrahim, Dino Amadori and Yibin Kang |
Title | Tumor-Induced Osteoclast miRNA Changes as Regulators and Biomarkers of Osteolytic Bone Metastasis. |
Journal | Cancer Cell. 2013. |
Authors | Ell B, Mercatali L, Ibrahim T, Campbell N, Schwarzenbach H, Pantel K, Amadori D, Kang Y. |
Title | Tyrosine kinase receptor status in endometrial stromal sarcoma: an immunohistochemical and genetic-molecular analysis. |
Journal | International Journal of Gynecology Pathology. 2012. |
Authors | Cossu-Rocca P, Contini M, Uras MG, Muroni MR, Pili F, Carru C, Bosincu L, Massarelli G, Nogales FF, De Miglio MR. |
Title | Nucleic acids from long-term preserved FFPE tissues are suitable for downstream analyses. |
Journal | Virchows Arch. 2012. |
Authors | Ludyga N, Grünwald B, Azimzadeh O, Englert S, Höfler H, Tapio S, Aubele M. |
Title | Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections. |
Journal | BMC Research Notes. 2012. |
Authors | Patnaik SK, Kannisto E, Yendamuri S. |
Title | Overexpression of the Lung Cancer-Prognostic miR-146b MicroRNAs Has a Minimal and Negative Effect on the Malignant Phenotype of A549 Lung Cancer Cells. |
Journal | PLoS One. 2011. |
Authors | Patnaik S, Kannisto E, Mallick R, Yendamuri S. |