Sequential isolation and purification of total RNA and genomic DNA from FFPE tissue samples

Formerly Cat. 25000

  • Fast and easy processing using rapid spin-column format
  • High yields and quality of nucleic acids
  • Separate fractionation of RNA and DNA
  • Isolate total RNA, from large rRNA down to microRNA (miRNA)
  • No phenol or chloroform extractions

ItemsCat. #Size
FFPE RNA/DNA Purification Plus Kit 54300 50 preps

FFPE RNA/DNA Purification Plus Kit

Norgen’s FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. The RNA and DNA are sequentially purified into separate fractions using this kit. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE RNA/DNA Purification Plus Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The purified genomic DNA is also of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting and SNP analysis.

Kit Specifications
Binding Capacity Per Column
RNA: Up to 35 μg
DNA: Up to 10 μg
Maximum Loading Volume Per Spin Column
600 μL
Size of RNA Purified

All sizes, including < 200 nt

Maximum Amount of Starting Material
4 sections <20 μM thick
10 mg of unsectioned block

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.

Kit Components
Component Cat. 54300 (50 preps)
Digestion Buffer A 2 x 25 mL
Buffer RL 40 mL
Enzyme Incubation Buffer A 6 mL
Wash Solution A 2 x 38 mL
Elution Solution A 6 mL
Elution Buffer F 15 mL
Proteinase K 2 x 12 mg
DNase I 1 vial
RNA Purification Micro Columns 50
DNA Purification Micro Columns 50
Collection Tubes 100
Elution Tubes (1.7 mL) 100
Product Insert 1

Title MicroRNA-34c-5p is related to recurrence in laryngeal squamous cell carcinoma
Journal The Laryngoscope. 2015.
Authors Massimo Re MD, Artan Çeka MD, Corrado Rubini MD, Luigi Ferrante PhD, Antonio Zizzi PhD, Federico M. Gioacchini MD, Michele Tulli MD, Liana Spazzafumo PhD, Stefano Sellari-Franceschini MD, Antonio D. Procopio MD, andFabiola Olivieri PhD
Title In-Vivo Fusion of Human Cancer and Hamster Stromal Cells Permanently Transduces and Transcribes Human DNA
Journal PLOS ONE. 2014.
Authors David M. Goldenberg, Robert J. Rooney, Meiyu Loo, Donglin Liu, Chien-Hsing Chang
Title The miRNA-23b/27b/24 Cluster Promotes Breast Cancer Lung Metastasis by Targeting Metastasis-Suppressive Gene Prosaposin.
Journal The Journal of Biological Chemistry. 2014.
Authors Brian Ell, Qiong Qiu, Yong Wei, Laura Mercatali, Toni Ibrahim, Dino Amadori and Yibin Kang
Title Tumor-Induced Osteoclast miRNA Changes as Regulators and Biomarkers of Osteolytic Bone Metastasis.
Journal Cancer Cell. 2013.
Authors Ell B, Mercatali L, Ibrahim T, Campbell N, Schwarzenbach H, Pantel K, Amadori D, Kang Y.
Title Tyrosine kinase receptor status in endometrial stromal sarcoma: an immunohistochemical and genetic-molecular analysis.
Journal International Journal of Gynecology Pathology. 2012.
Authors Cossu-Rocca P, Contini M, Uras MG, Muroni MR, Pili F, Carru C, Bosincu L, Massarelli G, Nogales FF, De Miglio MR.
Title Nucleic acids from long-term preserved FFPE tissues are suitable for downstream analyses.
Journal Virchows Arch. 2012.
Authors Ludyga N, Grünwald B, Azimzadeh O, Englert S, Höfler H, Tapio S, Aubele M.
Title Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections.
Journal BMC Research Notes. 2012.
Authors Patnaik SK, Kannisto E, Yendamuri S.