Human Papillomavirus (HPV) (High and Low Risk) TaqMan PCR Lyophilized Kits

Overview

Detection kits for HPV (High and Low Risk)
Lyophilized format designed for room temperature shipping
Available in TaqMan format for analysis

Norgen’s HPV (High and Low Risk) TaqMan PCR Lyophilized Kit is designed for the detection of HPV High and Low Risk specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Norgen’s HPV (High and Low Risk) TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of HPV High and Low Risk specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Details

Supporting Data

TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.

End-Point PCR Kit - Figure 4. A representative 1X TAE, 1.4 % agarose gel showing the amplification of HPV (High and Low Risk) at different concentrations. The size of the HPV (High and Low Risk) target amplicon corresponds to the 280 bp band represented by the provided DNA Marker (M).

Storage Conditions and Product Stability

All kit components should be stored at -20°C upon arrival.
Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

Component	Cat. TM31550L (100 preps)	Cat. TM31510L (100 preps)
MDx TaqMan 2X PCR Master Mix (Lyo)	4 x 350 μL	-
Mycoplasma Primer & Probe Mix (Lyo)	280 μL	280 μL
Mycoplasma Positive Control (Lyo)	150 μL	150 μL
Nuclease-Free Water (Negative Control)	3 x 1.25 mL	1 x 1.25 mL
Product Insert	1	1

Listed volumes of components marked with (Lyo) are the volumes upon reconstitution

Documentation

PROTOCOLS

(TM31510L) HPV (High and Low Risk) TaqMan Lyophilized Probe/Primer and Control Set - Protocol (100 Preps)

(TM31550L) HPV (High and Low Risk) TaqMan PCR Lyophilized Kit - Protocol (100 Preps)

SAFETY DATA SHEETS

TM31550L - HPV (High and Low Risk) TaqMan PCR Lyophilized Kit - SDS

TM31510L - HPV (High and Low Risk) TaqMan Lyophilized Probe-Primer and Control Set - SDS

Human Papillomavirus (HPV) (High and Low Risk) TaqMan PCR Lyophilized Kits