Human Papillomavirus (HPV) (High and Low Risk) TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Human Papillomavirus (HPV) (High and Low Risk) TaqMan PCR Detection Kits SMART Save USD$699.00 USD$559.20 SKU TM31550 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for the HPV (High and Low Risk) Available in TaqMan format for analysis More than 70 types of human papillomavirus (HPV) have been identified, and are generally classified as high-risk or low-risk depending on their relationship or lack of relationship with cancer and high-grade cervical intraepithelial neoplasia (CIN 2-3). HPV viruses are predominantly sexually transmitted and high-risk HPV types are a major risk factor for development of cervical cancer. Low-risk HPV types 6 and 11 have been associated with the presence of genital warts. There are many other low-risk HPV types that are not associated with genital warts or cervical cancer. Until now, HPV cannot be cultured in vitro, and immunological tests are inadequate to determine the presence of HPV cervical infection. On the other hand, biopsies can be analyzed by nucleic acid hybridization to directly detect the presence of HPV DNA. HPV 16 and HPV 18 have been considered as high-risk cancer associated HPV types. HPV types 31, 33, and 35 have been shown to have an intermediate relationship with cancer. Norgen's Human Papillomavirus (HPV) (High and Low Risk) TaqMan PCR Detection Kits are suitable for the detection of HPV 33 and HPV 58. TaqMan PCR Kit Click to expand options HPV (High and Low Risk) TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents HPV (High and Low Risk) TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. End-Point PCR Kit - Figure 4. A representative 1X TAE, 1.4 % agarose gel showing the amplification of HPV (High and Low Risk) at different concentrations. The size of the HPV (High and Low Risk) target amplicon corresponds to the 280 bp band represented by the provided DNA Marker (M). Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM31550 (100 preps) Cat. TM31510 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - HPV (High and Low Risk) Primer & Probe Mix 280 μL 280 μL HPV (High and Low Risk) Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM31550) HPV (High and Low Risk) TaqMan PCR Kit - Protocol (100 rxns) (TM31510) HPV High and Low Risk TaqMan Probe/Primer and Control Set - Protocol (100 rxns) SAFETY DATA SHEETS (TM31550) HPV (High and Low Risk) TaqMan PCR Kit - Safety Data Sheet Related Products Urine DNA Isolation Kit (Slurry Format)
Human Papillomavirus (HPV) (High and Low Risk) TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Human Papillomavirus (HPV) (High and Low Risk) TaqMan PCR Detection Kits SMART Save USD$699.00 USD$559.20 SKU TM31550 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity
