Plant/Fungi Total RNA Purification Kit For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plant/Fungi Total RNA Purification Kit SKU 25800 Format Spin Column High Throughput Size 50 Preps 100 Preps 250 Preps Quantity Trouver un distributeur Contact Overview Details Documentation Citations Aperçu du produit Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient. The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. Norgen's Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation. Détails Supporting Data Previous Figure 1. Isolation of High Quality RNA, even from Difficult Samples. Total RNA was isolated from 50 mg samples of apple (Lanes 1), peach (Lanes 2), grape (Lanes 3), pine needle (Lanes 4), strawberry (Lanes 5) and pear (Lanes 6) using Norgen's kit and a competitors kit. Five microlitres of total RNA from the 50 µL elution was loaded on 1X MOPS 1.0 % Formaldehyde-Agarose RNA gel for analysis. Norgen's kit allowed for the isolation of high quality RNA from all the samples, including the difficult samples, while the competitor failed to isolate RNA from grape, pine needles and strawberry. Furthermore, only Norgen's kit was able to isolate the small RNA species (white box). Figure 2. Detection of EF1-α using one step RT-qPCR from Challenging Plant Samples. Total RNA was isolated from 50 mg samples of apple (red), peach (green), pine needle (blue) and grape leaves (burgundy) using Norgen's Plant/Fungi Total RNA Purification Kit. Three µL of the eluted 50 µL of RNA was used in an RT-qPCR reaction for the detection of EF1-α. EF1-α was detected from all samples, indicating that the RNA is of high quality and that the Plant/Fungi RNA Purification kit is highly sensitive for total RNA isolation. Figure 3. High Yield of Plant RNA Samples isolated from Apple, Peach and Pear. Total RNA was isolated using Norgen's kit (Blue bars) and a competitor’s kit (Dark blue bars) from 50 mg of apple, peach and pear according to the provided manual. In each case the RNA was eluted into 50 µL. RNA yield was measured by spectrophotometer in triplicate, and the average concentration was used for data analysis. Norgen's kit resulted in higher yields of total RNA, including miRNA. Total processing time was less with Norgen's kit than competitor's kit. Figure 4. High Purity of RNA Samples Isolated from Apple, Peach and Pear. RNA was isolated from 50 mg samples of apple, peach and pear using Norgen's Plant/Fungi RNA Purification Kit and a competitor's kit. RNA purity was determined spectrophotometrically for the RNA isolated using Norgen's kit (260/230 = dark blue bars) and the competitor's kit (260/230 = light blue bars). Also shown is the 260/280 ratio for the samples. Norgen's kit consistently isolated pure samples of Plant RNA with 260/230 ratios above 2.0, whereas the competitor's kit had 260/230 ratios below 1.2 for the same samples. Next Click for expanded view Kit Specifications - Spin Column Maximum Column Binding Capacity 50 μg Maximum Column Loading Volume 650 μL Size of RNA Purified All sizes, including small RNA (< 200 nt) Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi 50 mg 1 x 106 cells 50 mg (wet weight) Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves 60 μg 60 μg 32 μg 35 μg 30 μg Time to Complete 10 Purifications 30 minutes * Yield will vary depending on the type of sample processed. * Yield will vary depending on the type of sample processed. Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits Plants Tobacco (Nicotiana tabacum) Tomato (Lycopersicon esculentum) Pepper (Capsicum annuum) Potato (Solanum tuberosum) Arabidopsis thaliana1 Peach (Prunus persica) Apple (Malus sp.) Pear (Pyrus sp.) Grape vine (Vitis sp.) Plum (Prunus sp.) Palm (Arecaceae) Pine needle (Pinaceae) Strawberry Raspberry Blackberry Herbs Persimmon (Ebenaceae) Potato tuber (Solanum) Plum fruit Citrus Vanilla bean Cotton (Gossypium) Mangrove Chrysanthemum Grape berry skin Kiwi leaves Peach (fruits and flowers) Soy bean (legume) Eastern White Red Cedar Corn leaves Cucumber leaves Fungi Aspergillus niger Mucor racemosus Cladosporium cladosporioides Fusarium oxysporum Penicillium sp. Botrytis cinerea (Botryotinia fuckeliana) Pichia sp. Rhizopus oryzae Alternaria tenuissima Component Cat. 25800 (50 preps) Cat. 31350 (100 preps) Cat. 25850 (250 preps) Cat. 31900 (192 preps) Lysis Buffer C 60 mL 1 x 30 mL, 1 x 60 mL 3 x 60 mL 2 x 60 mL Wash Solution A 38 mL 38 mL 1 x 18 mL, 2 x 38 mL 2 x 38 mL Elution Solution A 6 mL 6 mL 20 mL 20 mL Filter Columns 50 100 250 - Spin Columns 50 100 250 - 96-Well Plate - - - 2 Adhesive Tape - - - 4 Collection Tubes 100 200 500 - 96-Well Collection Plate - - - 2 Elution Tubes (1.7 mL) 50 100 250 - 96-Well Elution Plate - - - 2 Product Insert 1 1 1 1 Documentation PROTOCOLS (25800) Plant/Fungi Total RNA Purification Kit (Spin Column) - Protocol (50 Preps) (31350) Plant/Fungi Total RNA Purification Kit (Spin Column) - Protocol (100 Preps) (25850) Plant/Fungi Total RNA Purification Kit (Spin Column) - Protocol (250 Preps) (31900) Plant/Fungi Total RNA Purification 96-Well Kit (HT) - Protocol (96-well) SUPPLEMENTARY PROTOCOLS Supplementary Protocol for Plant Tissue Homogenization Without Liquid Nitrogen Grinding APPLICATION NOTES Isolation of Plant and Fungal RNA from Challenging Samples using Norgen’s Plant/Fungi RNA Purification Kit Non-Organic-Based Isolation of Plant microRNA using Norgen’s Plant/Fungi RNA Purification Kit Broad Application of a Single Universal Lysis Buffer for True Total RNA Purification from Challenging Plant Species and Tissues Evaluation of Plant RNA Integrity Number (RIN) generated using an Agilent BioAnalyzer 2100 Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input Optimizing Bead Homogenization of Plant Tissues for DNA and RNA Isolation Preservation of RNA in Soil, Plant and Stool Samples Using Norgen's RNA Preserve Sensitivity and Specificity of Norgen’s HLVd TaqMan RT-PCR Kit.pdf SAFETY DATA SHEETS (25800, 31350) Plant/Fungi Total RNA Purification Kit - Safety Data Sheet POSTERS A Novel Technology To Extract Nucleic Acids From Different Environmental Samples Including Plants Water and Soil - Poster Revised Guidelines For RNA Quality Assessment For Diverse Biological Sample Input - Poster Compatibility of DNA and RNA Extraction Methods For Challenging Plant Species - Poster A New Gold Standard for Viroid Detection: Comparison Study Between Membrane Hybridization and RT-PCR Rapid Isolation and Sensitive Detection of the Chrysanthemum Chlorotic Mottle Viroid Development of a high throughput PPV detection method based on unique nucleic acid isolation system The Importance of Sample Preparation for Plant miRNA Purification Assessing the sensitivity and specificity of three different methods for DNA isolation from Borrelia burgdorferi FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Related Products Plant/Fungi DNA Isolation Kit RNase-Free DNase I Kit Total RNA Purification Kit
Plant/Fungi Total RNA Purification Kit For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plant/Fungi Total RNA Purification Kit SKU 25800 Format Spin Column High Throughput Size 50 Preps 100 Preps 250 Preps Quantity Trouver un distributeur Contact