Plant/Fungi Total RNA Purification Kit
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For research use only and NOT intended for in vitro diagnostics.
Aperçu du produit
Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient.
The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen's Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
Détails
Supporting Data
Figure 1. Isolation of High Quality RNA, even from Difficult Samples. Total RNA was isolated from 50 mg samples of apple (Lanes 1), peach (Lanes 2), grape (Lanes 3), pine needle (Lanes 4), strawberry (Lanes 5) and pear (Lanes 6) using Norgen's kit and a competitors kit. Five microlitres of total RNA from the 50 µL elution was loaded on 1X MOPS 1.0 % Formaldehyde-Agarose RNA gel for analysis. Norgen's kit allowed for the isolation of high quality RNA from all the samples, including the difficult samples, while the competitor failed to isolate RNA from grape, pine needles and strawberry. Furthermore, only Norgen's kit was able to isolate the small RNA species (white box).
Figure 2. Detection of EF1-α using one step RT-qPCR from Challenging Plant Samples. Total RNA was isolated from 50 mg samples of apple (red), peach (green), pine needle (blue) and grape leaves (burgundy) using Norgen's Plant/Fungi Total RNA Purification Kit. Three µL of the eluted 50 µL of RNA was used in an RT-qPCR reaction for the detection of EF1-α. EF1-α was detected from all samples, indicating that the RNA is of high quality and that the Plant/Fungi RNA Purification kit is highly sensitive for total RNA isolation.
Figure 3. High Yield of Plant RNA Samples isolated from Apple, Peach and Pear. Total RNA was isolated using Norgen's kit (Blue bars) and a competitor’s kit (Dark blue bars) from 50 mg of apple, peach and pear according to the provided manual. In each case the RNA was eluted into 50 µL. RNA yield was measured by spectrophotometer in triplicate, and the average concentration was used for data analysis. Norgen's kit resulted in higher yields of total RNA, including miRNA. Total processing time was less with Norgen's kit than competitor's kit.
Figure 4. High Purity of RNA Samples Isolated from Apple, Peach and Pear. RNA was isolated from 50 mg samples of apple, peach and pear using Norgen's Plant/Fungi RNA Purification Kit and a competitor's kit. RNA purity was determined spectrophotometrically for the RNA isolated using Norgen's kit (260/230 = dark blue bars) and the competitor's kit (260/230 = light blue bars). Also shown is the 260/280 ratio for the samples. Norgen's kit consistently isolated pure samples of Plant RNA with 260/230 ratios above 2.0, whereas the competitor's kit had 260/230 ratios below 1.2 for the same samples.
Kit Specifications - Spin Column
|
|
Maximum Column Binding Capacity
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50 μg
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Maximum Column Loading Volume
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650 μL
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Size of RNA Purified
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All sizes, including small RNA (< 200 nt) |
Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi |
50 mg 1 x 106 cells 50 mg (wet weight) |
Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves |
60 μg 60 μg 32 μg 35 μg 30 μg |
Time to Complete 10 Purifications |
30 minutes
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* Yield will vary depending on the type of sample processed.
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Select Plants and Fungi Tested with the Norgen Plant/Fungi Total RNA Purification Kits
Plants
Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves
Fungi
Aspergillus nigerMucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima
Component | Cat. 25800 (50 preps) | Cat. 31350 (100 preps) | Cat. 25850 (250 preps) | Cat. 31900 (192 preps) |
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Lysis Buffer C | 60 mL | 1 x 30 mL, 1 x 60 mL | 3 x 60 mL | 2 x 60 mL |
Wash Solution A | 38 mL | 38 mL | 1 x 18 mL, 2 x 38 mL | 2 x 38 mL |
Elution Solution A | 6 mL | 6 mL | 20 mL | 20 mL |
Filter Columns | 50 | 100 | 250 | - |
Spin Columns | 50 | 100 | 250 | - |
96-Well Plate | - | - | - | 2 |
Adhesive Tape | - | - | - | 4 |
Collection Tubes | 100 | 200 | 500 | - |
96-Well Collection Plate | - | - | - | 2 |
Elution Tubes (1.7 mL) | 50 | 100 | 250 | - |
96-Well Elution Plate | - | - | - | 2 |
Product Insert | 1 | 1 | 1 | 1 |
Documentation
(31350) Plant/Fungi Total RNA Purification Kit (Spin Column) - Protocol (100 Preps)
(25850) Plant/Fungi Total RNA Purification Kit (Spin Column) - Protocol (250 Preps)
(31900) Plant/Fungi Total RNA Purification 96-Well Kit (HT) - Protocol (96-well)
Non-Organic-Based Isolation of Plant microRNA using Norgen’s Plant/Fungi RNA Purification Kit
Broad Application of a Single Universal Lysis Buffer for True Total RNA Purification from Challenging Plant Species and Tissues
Evaluation of Plant RNA Integrity Number (RIN) generated using an Agilent BioAnalyzer 2100
Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input
Optimizing Bead Homogenization of Plant Tissues for DNA and RNA Isolation
Preservation of RNA in Soil, Plant and Stool Samples Using Norgen's RNA Preserve
Sensitivity and Specificity of Norgen’s HLVd TaqMan RT-PCR Kit.pdf
Revised Guidelines For RNA Quality Assessment For Diverse Biological Sample Input - Poster
Compatibility of DNA and RNA Extraction Methods For Challenging Plant Species - Poster
A New Gold Standard for Viroid Detection: Comparison Study Between Membrane Hybridization and RT-PCR
Rapid Isolation and Sensitive Detection of the Chrysanthemum Chlorotic Mottle Viroid
Development of a high throughput PPV detection method based on unique nucleic acid isolation system
The Importance of Sample Preparation for Plant miRNA Purification
Assessing the sensitivity and specificity of three different methods for DNA isolation from Borrelia burgdorferi