Saliva DNA Isolation Kit Dx
For the rapid purification of high-quality DNA from preserved and fresh saliva samples for in vitro diagnostic use
For the rapid purification of high-quality DNA from preserved and fresh saliva samples for in vitro diagnostic use
For the rapid purification of high-quality DNA from preserved and fresh saliva samples for in vitro diagnostic use
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This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. 49000), as well as fresh saliva samples.
Saliva DNA purified using Norgen’s Saliva DNA Isolation Kit Dx kit is of the highest quality, and can be used with any downstream in vitro diagnostic application employing enzymatic amplification or other enzymatic modifications of DNA followed by signal detection or amplification.
Background
Saliva represents an excellent non-invasive alternative to blood collection. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.
NOTE: This product is not available for sale in the United States.
Kit Specifications
|
|
Maximum Saliva Input
|
0.5 mL preserved saliva
0.25 mL fresh saliva |
Average Yield from 0.25 mL of Saliva*
|
3 - 7 μg
|
Average Purity (OD260/280)
|
1.7 - 2.1
|
Time to Complete 10 Purifications |
30 minutes
|
* Average yield will depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels. The Proteinase K can be stored at either room temperature or 4oC.
Component | Cat. Dx45400 (50 preps) |
---|---|
Lysis Solution | 30 mL |
Proteinase K in Storage Buffer | 1.2 mL |
Binding Solution | 12 mL |
Wash Solution | 18 mL |
Elution Buffer | 12 mL |
Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
Title | Elucidating causative gene variants in hereditary Parkinson's disease in the Global Parkinson's Genetics Program (GP2) |
Citation | brief communications 2023. |
Authors | Lara M. Lange, Micol Avenali, Melina Ellis, Anastasia Illarionova, Ignacio J. Keller Sarmiento, Ai-Huey Tan, Harutyun Madoev, Caterina Galandra, Johanna Junker, Karisha Roopnarain, Justin Solle, Claire Wegel, Zih-Hua Fang, Peter Heutink, Kishore R. Kumar, Shen-Yang Lim, Enza Maria Valente, Mike Nalls, Cornelis Blauwendraat, Andrew Singleton, Niccolo Mencacci, Katja Lohmann, Christine Klein & the Global Parkinson's Genetic Program |