Saliva DNA Isolation Kit (Cat. RU45400)
Saliva DNA Isolation Kit
![Saliva DNA Isolation Kit](https://norgenbiotek.com/sites/default/files/styles/original/public/45400-Reflection-web-2.png?itok=d5cXY1Gp" "Saliva DNA Isolation Kit")
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What Scientists are Saying About this Product
We have been using Norgen's Saliva DNA Collection and Preservation Devices Cat. RU49000 and the Norgen's Saliva DNA Isolation Kit (Cat. RU45400) for many years for our world wide projects that include populations from different countries with very challenging collection and storage conditions. The kits have helped us even when the samples had to stay at tropical room temperature for long period of time. The kits worked perfectly for us. We also use the kits in our mail-based DNA collections to outreach the distant communities in Western Australia to offer people the opportunity to participate in genomic research. This way the Norgen kits help us to engage underrepresented populations and communities in our genomic studies.
-
Murdoch university
We have been using Norgen's Saliva DNA Collection and Preservation Devices Cat. RU49000 and the Norgen's Saliva DNA Isolation Kit (Cat. RU45400) for many years for our world wide projects that include populations from different countries with very challenging collection and storage conditions. The kits have helped us even when the samples had to stay at tropical room temperature for long period of time. The kits worked perfectly for us. We also use the kits in our mail-based DNA collections to outreach the distant communities in Western Australia to offer people the opportunity to participate in genomic research. This way the Norgen kits help us to engage underrepresented populations and communities in our genomic studies.
- Murdoch university
Saliva DNA Isolation Kit
For the rapid purification of high-quality DNA from preserved and fresh saliva samples
- Sample collection is non-invasive and painless
- Fast and easy processing using a rapid spin-column format
- Isolate high quality genomic DNA
- Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples
For research use only and NOT intended for in vitro diagnostics.
Diagnostic version available here
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(Cat. RU45400) 50 Preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
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Please note that Norgen Biotek provides sample kits exclusively to residents of North America. Customers outside of North America may request a sample from one of our global distributors. Find your local distributor.
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Saliva Input
|
0.5 mL preserved saliva
0.25 mL fresh saliva |
|
Average Yield from 0.25 mL of Saliva*
|
3 - 7 μg
|
|
Average Purity (OD260/280)
|
1.7 - 2.1
|
| Time to Complete 10 Purifications |
30 minutes
|
* Average yield will depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Supporting Data
| Component | Cat. RU45400 (50 preps) |
|---|---|
| Lysis Buffer F | 30 mL |
| Proteinase K in Storage Buffer | 1.2 mL |
| Binding Buffer B | 12 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 15 mL |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | TAS1R3 and TAS2R38 Polymorphisms Affect Sweet Taste Perception: An Observational Study on Healthy and Obese Subjects |
| Journal | Nutrients. 2022. |
| Authors | Monia Cecati, Arianna Vignini, Francesca Borroni, Sofia Pugnaloni, Sonila Alia, Jacopo Sabbatinelli, Giulia Nicolai, Marina Taus, Andrea Santarelli, Mara Fabri, Laura Mazzanti and Monica Emanuelli |
| Title | At age 9, the methylome of assisted reproductive technology children that underwent embryo culture in different media is not significantly different on a genome-wide scale |
| Journal | Human Reproduction. 2022. |
| Authors | Rebekka M Koeck, Florence Busato, Jorg Tost, Heleen Zandstra, Sylvie Remy, Sabine Langie, Marij Gielen, Ron van Golde, John C M Dumoulin, Han Brunner, Masoud Zamani Esteki, Aafke P A van Montfoort |
| Title | Functional validation of UGT1A6 in anthracycline-induced cardiotoxicity |
| Journal | UBC Theses and Dissertations. 2022. |
| Authors | Morrison, Alexander |
| Title | FOK l Vitamin D Receptor Gene Polymorphism and Risk of Dental Caries: A Case-Control Study |
| Journal | International Journal of Dentistry. 2022. |
| Authors | Nireeksha Nireeksha, Mithra N. Hegde, Shilpa S. Shetty, and Suchetha N. Kumari |
| Title | Analysis of the Prader–Willi syndrome imprinting center using droplet digital PCR and next‐generation whole‐exome sequencing |
| Journal | Molecular Genetics and Genomic Medicine. 2019. |
| Authors | Samantha N. Hartin, Waheeda A. Hossain, David Francis, David E. Godler, Sangjucta Barkataki and Merlin G. Butler |
| Title | Associations between the oxytocin receptor gene (OXTR) rs53576 polymorphism and emotional processing of social and nonsocial cues: an event-related potential (ERP) study |
| Journal | Journal of Physiological Anthropology. 2017. |
| Authors | Choi D, Minote N, Watanuki S. |
| Title | The myth of oral hygiene using synthetic mouthwash products |
| Journal | SpringerPlus. 2016. |
| Authors | Ghulam Zahara Jahangir, Durre Shahwar Ashraf, Idrees Ahmad Nasir, Madeha Sadiq, Sobia Shahzad, Farah Naz, Muhammad Iqbal and Afifa Saeed |
| Title | Case-control study of glucocorticoid receptor and corticotrophin-releasing hormone receptor gene variants and risk of perinatal depression |
| Journal | BMC Pregnancy and Childbirth. 2015. |
| Authors | Ene-Choo Tan, Tze-Ern Chua, Theresa M. Y. Lee, Hui-San Tan, Joe L. Y. Ting and Helen Y. Chen |
| Title | First Detection of Antibodies Against African Swine Fever Virus in Faeces Samples |
| Journal | Transboundary and Emerging Diseases. 2015. |
| Authors | E. Nieto-Pelegrın, B. Rivera-Arroyo and J. M. Sanchez-Vizcaıno |
| Title | 1000 Norms Project: protocol of a cross-sectional study cataloging human variation |
| Journal | Physiotherapy. 2015. |
| Authors | Marnee J. McKay, Jennifer N. Baldwin, Paulo Ferreira, Milena Simic, Natalie Vanicek, Claire E. Hiller, Elizabeth J. Nightingale, Niamh A. Moloney, Kate G. Quinlan, Fereshteh Pourkazemi, Amy D. Sman, Leslie L. Nicholson, Seyed J. Mousavi, Kristy Rose, Jacqueline Raymond, Martin G. Mackey, Angus Chard, Markus Hübscher, Caleb Wegener, Alycia Fong Yan, Kathryn M. Refshauge, Joshua Burns |
| Title | TAS2R38 single nucleotide polymorphisms are associated with PROP- but not thermal tasting: a pilot study. |
| Journal | Chemosensory Perception. 2013. |
| Authors | Bering A, Pickering G, Liang P. |
| Title | Brief communication: Evolution of a specific O allele (O1v(G542A) ) supports unique ancestry of Native Americans. |
| Journal | American Journal of Physical Anthropology. 2013. |
| Authors | Villanea FA, Bolnick DA, Monroe C, Worl R, Cambra R, Leventhal A, Kemp BM. |
| Title | Differences in the quantity of DNA found in the urine and saliva of smokers versus nonsmokers: implications for the timing of epigenetic events. |
| Journal | Epigenomics. 2012. |
| Authors | Simkin M, Abdalla M, El-Mogy M, Haj-Ahmad Y. |
Saliva DNA Isolation Kit
This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples.
Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.
Background
Saliva represents an excellent non-invasive alternative to blood collection. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Saliva Input
|
0.5 mL preserved saliva
0.25 mL fresh saliva |
|
Average Yield from 0.25 mL of Saliva*
|
3 - 7 μg
|
|
Average Purity (OD260/280)
|
1.7 - 2.1
|
| Time to Complete 10 Purifications |
30 minutes
|
* Average yield will depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. RU45400 (50 preps) |
|---|---|
| Lysis Buffer F | 30 mL |
| Proteinase K in Storage Buffer | 1.2 mL |
| Binding Buffer B | 12 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 15 mL |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | TAS1R3 and TAS2R38 Polymorphisms Affect Sweet Taste Perception: An Observational Study on Healthy and Obese Subjects |
| Journal | Nutrients. 2022. |
| Authors | Monia Cecati, Arianna Vignini, Francesca Borroni, Sofia Pugnaloni, Sonila Alia, Jacopo Sabbatinelli, Giulia Nicolai, Marina Taus, Andrea Santarelli, Mara Fabri, Laura Mazzanti and Monica Emanuelli |
| Title | At age 9, the methylome of assisted reproductive technology children that underwent embryo culture in different media is not significantly different on a genome-wide scale |
| Journal | Human Reproduction. 2022. |
| Authors | Rebekka M Koeck, Florence Busato, Jorg Tost, Heleen Zandstra, Sylvie Remy, Sabine Langie, Marij Gielen, Ron van Golde, John C M Dumoulin, Han Brunner, Masoud Zamani Esteki, Aafke P A van Montfoort |
| Title | Functional validation of UGT1A6 in anthracycline-induced cardiotoxicity |
| Journal | UBC Theses and Dissertations. 2022. |
| Authors | Morrison, Alexander |
| Title | FOK l Vitamin D Receptor Gene Polymorphism and Risk of Dental Caries: A Case-Control Study |
| Journal | International Journal of Dentistry. 2022. |
| Authors | Nireeksha Nireeksha, Mithra N. Hegde, Shilpa S. Shetty, and Suchetha N. Kumari |
| Title | Analysis of the Prader–Willi syndrome imprinting center using droplet digital PCR and next‐generation whole‐exome sequencing |
| Journal | Molecular Genetics and Genomic Medicine. 2019. |
| Authors | Samantha N. Hartin, Waheeda A. Hossain, David Francis, David E. Godler, Sangjucta Barkataki and Merlin G. Butler |
| Title | Associations between the oxytocin receptor gene (OXTR) rs53576 polymorphism and emotional processing of social and nonsocial cues: an event-related potential (ERP) study |
| Journal | Journal of Physiological Anthropology. 2017. |
| Authors | Choi D, Minote N, Watanuki S. |
| Title | The myth of oral hygiene using synthetic mouthwash products |
| Journal | SpringerPlus. 2016. |
| Authors | Ghulam Zahara Jahangir, Durre Shahwar Ashraf, Idrees Ahmad Nasir, Madeha Sadiq, Sobia Shahzad, Farah Naz, Muhammad Iqbal and Afifa Saeed |
| Title | Case-control study of glucocorticoid receptor and corticotrophin-releasing hormone receptor gene variants and risk of perinatal depression |
| Journal | BMC Pregnancy and Childbirth. 2015. |
| Authors | Ene-Choo Tan, Tze-Ern Chua, Theresa M. Y. Lee, Hui-San Tan, Joe L. Y. Ting and Helen Y. Chen |
| Title | First Detection of Antibodies Against African Swine Fever Virus in Faeces Samples |
| Journal | Transboundary and Emerging Diseases. 2015. |
| Authors | E. Nieto-Pelegrın, B. Rivera-Arroyo and J. M. Sanchez-Vizcaıno |
| Title | 1000 Norms Project: protocol of a cross-sectional study cataloging human variation |
| Journal | Physiotherapy. 2015. |
| Authors | Marnee J. McKay, Jennifer N. Baldwin, Paulo Ferreira, Milena Simic, Natalie Vanicek, Claire E. Hiller, Elizabeth J. Nightingale, Niamh A. Moloney, Kate G. Quinlan, Fereshteh Pourkazemi, Amy D. Sman, Leslie L. Nicholson, Seyed J. Mousavi, Kristy Rose, Jacqueline Raymond, Martin G. Mackey, Angus Chard, Markus Hübscher, Caleb Wegener, Alycia Fong Yan, Kathryn M. Refshauge, Joshua Burns |
| Title | TAS2R38 single nucleotide polymorphisms are associated with PROP- but not thermal tasting: a pilot study. |
| Journal | Chemosensory Perception. 2013. |
| Authors | Bering A, Pickering G, Liang P. |
| Title | Brief communication: Evolution of a specific O allele (O1v(G542A) ) supports unique ancestry of Native Americans. |
| Journal | American Journal of Physical Anthropology. 2013. |
| Authors | Villanea FA, Bolnick DA, Monroe C, Worl R, Cambra R, Leventhal A, Kemp BM. |
| Title | Differences in the quantity of DNA found in the urine and saliva of smokers versus nonsmokers: implications for the timing of epigenetic events. |
| Journal | Epigenomics. 2012. |
| Authors | Simkin M, Abdalla M, El-Mogy M, Haj-Ahmad Y. |
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