For the rapid purification of high-quality DNA from preserved and fresh saliva samples

  • Sample collection is non-invasive and painless
  • Fast and easy processing using a rapid spin-column format
  • Isolate high quality genomic DNA
  • Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples

ItemsCat. #Size
Saliva DNA Isolation Kit RU45400 50 Preps

Saliva DNA Isolation Kit

This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples.

Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.

Background

Saliva represents an excellent non-invasive alternative to blood collection. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.

Kit Specifications
Maximum Saliva Input
0.5 mL preserved saliva
0.25 mL fresh saliva
Average Yield from 0.25 mL of Saliva*
3 - 7 μg
Average Purity (OD260/280)
1.7 - 2.1
Time to Complete 10 Purifications
30 minutes

* Average yield will depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers. The Proteinase K can be stored at either room temperature or 4oC.


Kit Components
Component Cat. RU45400 (50 preps)
Lysis Buffer F 30 mL
Proteinase K 1.2 mL
Binding Buffer B 12 mL
Wash Solution A 18 mL
Elution Buffer B 15 mL
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title 1000 Norms Project: protocol of a cross-sectional study cataloging human variation
Journal Physiotherapy. 2015.
Authors Marnee J. McKay, Jennifer N. Baldwin, Paulo Ferreira, Milena Simic, Natalie Vanicek, Claire E. Hiller, Elizabeth J. Nightingale, Niamh A. Moloney, Kate G. Quinlan, Fereshteh Pourkazemi, Amy D. Sman, Leslie L. Nicholson, Seyed J. Mousavi, Kristy Rose, Jacqueline Raymond, Martin G. Mackey, Angus Chard, Markus Hübscher, Caleb Wegener, Alycia Fong Yan, Kathryn M. Refshauge, Joshua Burns
Title TAS2R38 single nucleotide polymorphisms are associated with PROP- but not thermal tasting: a pilot study.
Journal Chemosensory Perception. 2013.
Authors Bering A, Pickering G, Liang P.
Title Brief communication: Evolution of a specific O allele (O1v(G542A) ) supports unique ancestry of Native Americans.
Journal American Journal of Physical Anthropology. 2013.
Authors Villanea FA, Bolnick DA, Monroe C, Worl R, Cambra R, Leventhal A, Kemp BM.