Saliva DNA Isolation Kit

Cat. RU45400
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Saliva DNA Isolation Kit

Cat. RU45400

For the rapid purification of high-quality DNA from preserved and fresh saliva samples

  • Sample collection is non-invasive and painless
  • Fast and easy processing using a rapid spin-column format
  • Isolate high quality genomic DNA
  • Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples

For research use only and NOT intended for in vitro diagnostics.

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Saliva DNA Isolation Kit
(Cat. RU45400)
50 Preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Sample collection is non-invasive and painless
  • Fast and easy processing using a rapid spin-column format
  • Isolate high quality genomic DNA
  • Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples

Saliva DNA Isolation Kit

This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples.

Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.

Background

Saliva represents an excellent non-invasive alternative to blood collection.  Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.

 

 

Kit Specifications

You have selected: Cat. RU45400
Kit Specifications
Maximum Saliva Input
0.5 mL preserved saliva
0.25 mL fresh saliva
Average Yield from 0.25 mL of Saliva*
3 - 7 μg
Average Purity (OD260/280)
1.7 - 2.1
Time to Complete 10 Purifications
30 minutes

* Average yield will depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Supporting Data

Click for expanded view

Component Cat. RU45400 (50 preps)
Lysis Buffer F 30 mL
Proteinase K 1.2 mL
Binding Buffer B 12 mL
Wash Solution A 18 mL
Elution Buffer B 15 mL
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title TAS1R3 and TAS2R38 Polymorphisms Affect Sweet Taste Perception: An Observational Study on Healthy and Obese Subjects
Journal Nutrients. 2022.
Authors Monia Cecati, Arianna Vignini, Francesca Borroni, Sofia Pugnaloni, Sonila Alia, Jacopo Sabbatinelli, Giulia Nicolai, Marina Taus, Andrea Santarelli, Mara Fabri, Laura Mazzanti and Monica Emanuelli
Title At age 9, the methylome of assisted reproductive technology children that underwent embryo culture in different media is not significantly different on a genome-wide scale
Journal Human Reproduction. 2022.
Authors Rebekka M Koeck, Florence Busato, Jorg Tost, Heleen Zandstra, Sylvie Remy, Sabine Langie, Marij Gielen, Ron van Golde, John C M Dumoulin, Han Brunner, Masoud Zamani Esteki, Aafke P A van Montfoort
Title Functional validation of UGT1A6 in anthracycline-induced cardiotoxicity
Journal UBC Theses and Dissertations. 2022.
Authors Morrison, Alexander
Title FOK l Vitamin D Receptor Gene Polymorphism and Risk of Dental Caries: A Case-Control Study
Journal International Journal of Dentistry. 2022.
Authors Nireeksha Nireeksha, Mithra N. Hegde, Shilpa S. Shetty, and Suchetha N. Kumari
Title Analysis of the Prader–Willi syndrome imprinting center using droplet digital PCR and next‐generation whole‐exome sequencing
Journal Molecular Genetics and Genomic Medicine. 2019.
Authors Samantha N. Hartin, Waheeda A. Hossain, David Francis, David E. Godler, Sangjucta Barkataki and Merlin G. Butler
Title The myth of oral hygiene using synthetic mouthwash products
Journal SpringerPlus. 2016.
Authors Ghulam Zahara Jahangir, Durre Shahwar Ashraf, Idrees Ahmad Nasir, Madeha Sadiq, Sobia Shahzad, Farah Naz, Muhammad Iqbal and Afifa Saeed
Title Case-control study of glucocorticoid receptor and corticotrophin-releasing hormone receptor gene variants and risk of perinatal depression
Journal BMC Pregnancy and Childbirth. 2015.
Authors Ene-Choo Tan, Tze-Ern Chua, Theresa M. Y. Lee, Hui-San Tan, Joe L. Y. Ting and Helen Y. Chen
Title First Detection of Antibodies Against African Swine Fever Virus in Faeces Samples
Journal Transboundary and Emerging Diseases. 2015.
Authors E. Nieto-Pelegrın, B. Rivera-Arroyo and J. M. Sanchez-Vizcaıno
Title 1000 Norms Project: protocol of a cross-sectional study cataloging human variation
Journal Physiotherapy. 2015.
Authors Marnee J. McKay, Jennifer N. Baldwin, Paulo Ferreira, Milena Simic, Natalie Vanicek, Claire E. Hiller, Elizabeth J. Nightingale, Niamh A. Moloney, Kate G. Quinlan, Fereshteh Pourkazemi, Amy D. Sman, Leslie L. Nicholson, Seyed J. Mousavi, Kristy Rose, Jacqueline Raymond, Martin G. Mackey, Angus Chard, Markus Hübscher, Caleb Wegener, Alycia Fong Yan, Kathryn M. Refshauge, Joshua Burns
Title TAS2R38 single nucleotide polymorphisms are associated with PROP- but not thermal tasting: a pilot study.
Journal Chemosensory Perception. 2013.
Authors Bering A, Pickering G, Liang P.
Title Brief communication: Evolution of a specific O allele (O1v(G542A) ) supports unique ancestry of Native Americans.
Journal American Journal of Physical Anthropology. 2013.
Authors Villanea FA, Bolnick DA, Monroe C, Worl R, Cambra R, Leventhal A, Kemp BM.

Saliva DNA Isolation Kit

This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples.

Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.

Background

Saliva represents an excellent non-invasive alternative to blood collection.  Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.

 

 

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. RU45400
Kit Specifications
Maximum Saliva Input
0.5 mL preserved saliva
0.25 mL fresh saliva
Average Yield from 0.25 mL of Saliva*
3 - 7 μg
Average Purity (OD260/280)
1.7 - 2.1
Time to Complete 10 Purifications
30 minutes

* Average yield will depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component Cat. RU45400 (50 preps)
Lysis Buffer F 30 mL
Proteinase K 1.2 mL
Binding Buffer B 12 mL
Wash Solution A 18 mL
Elution Buffer B 15 mL
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title TAS1R3 and TAS2R38 Polymorphisms Affect Sweet Taste Perception: An Observational Study on Healthy and Obese Subjects
Journal Nutrients. 2022.
Authors Monia Cecati, Arianna Vignini, Francesca Borroni, Sofia Pugnaloni, Sonila Alia, Jacopo Sabbatinelli, Giulia Nicolai, Marina Taus, Andrea Santarelli, Mara Fabri, Laura Mazzanti and Monica Emanuelli
Title At age 9, the methylome of assisted reproductive technology children that underwent embryo culture in different media is not significantly different on a genome-wide scale
Journal Human Reproduction. 2022.
Authors Rebekka M Koeck, Florence Busato, Jorg Tost, Heleen Zandstra, Sylvie Remy, Sabine Langie, Marij Gielen, Ron van Golde, John C M Dumoulin, Han Brunner, Masoud Zamani Esteki, Aafke P A van Montfoort
Title Functional validation of UGT1A6 in anthracycline-induced cardiotoxicity
Journal UBC Theses and Dissertations. 2022.
Authors Morrison, Alexander
Title FOK l Vitamin D Receptor Gene Polymorphism and Risk of Dental Caries: A Case-Control Study
Journal International Journal of Dentistry. 2022.
Authors Nireeksha Nireeksha, Mithra N. Hegde, Shilpa S. Shetty, and Suchetha N. Kumari
Title Analysis of the Prader–Willi syndrome imprinting center using droplet digital PCR and next‐generation whole‐exome sequencing
Journal Molecular Genetics and Genomic Medicine. 2019.
Authors Samantha N. Hartin, Waheeda A. Hossain, David Francis, David E. Godler, Sangjucta Barkataki and Merlin G. Butler
Title The myth of oral hygiene using synthetic mouthwash products
Journal SpringerPlus. 2016.
Authors Ghulam Zahara Jahangir, Durre Shahwar Ashraf, Idrees Ahmad Nasir, Madeha Sadiq, Sobia Shahzad, Farah Naz, Muhammad Iqbal and Afifa Saeed
Title Case-control study of glucocorticoid receptor and corticotrophin-releasing hormone receptor gene variants and risk of perinatal depression
Journal BMC Pregnancy and Childbirth. 2015.
Authors Ene-Choo Tan, Tze-Ern Chua, Theresa M. Y. Lee, Hui-San Tan, Joe L. Y. Ting and Helen Y. Chen
Title First Detection of Antibodies Against African Swine Fever Virus in Faeces Samples
Journal Transboundary and Emerging Diseases. 2015.
Authors E. Nieto-Pelegrın, B. Rivera-Arroyo and J. M. Sanchez-Vizcaıno
Title 1000 Norms Project: protocol of a cross-sectional study cataloging human variation
Journal Physiotherapy. 2015.
Authors Marnee J. McKay, Jennifer N. Baldwin, Paulo Ferreira, Milena Simic, Natalie Vanicek, Claire E. Hiller, Elizabeth J. Nightingale, Niamh A. Moloney, Kate G. Quinlan, Fereshteh Pourkazemi, Amy D. Sman, Leslie L. Nicholson, Seyed J. Mousavi, Kristy Rose, Jacqueline Raymond, Martin G. Mackey, Angus Chard, Markus Hübscher, Caleb Wegener, Alycia Fong Yan, Kathryn M. Refshauge, Joshua Burns
Title TAS2R38 single nucleotide polymorphisms are associated with PROP- but not thermal tasting: a pilot study.
Journal Chemosensory Perception. 2013.
Authors Bering A, Pickering G, Liang P.
Title Brief communication: Evolution of a specific O allele (O1v(G542A) ) supports unique ancestry of Native Americans.
Journal American Journal of Physical Anthropology. 2013.
Authors Villanea FA, Bolnick DA, Monroe C, Worl R, Cambra R, Leventhal A, Kemp BM.

 


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