Saliva DNA Isolation Kit For the rapid purification of high-quality DNA from preserved and fresh saliva samples For research use only and NOT intended for in vitro diagnostics.Diagnostic version available here Powered by Bioz Saliva DNA Isolation Kit For the rapid purification of high-quality DNA from preserved and fresh saliva samples Price USD$169.00 SKU RU45400 Format Spin Column Size 50 Preps Quantity Overview Details Documentation FAQs Citations Overview Sample collection is non-invasive and painless Fast and easy processing using a rapid spin-column format Isolate high quality genomic DNA Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples.Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.BackgroundSaliva represents an excellent non-invasive alternative to blood collection. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples. Details Supporting Data Previous Figure 1. High Quality and Yield of DNA from Saliva Samples. Total DNA was isolated from 250 µL of eight different fresh saliva samples using Norgen's Saliva DNA Isolation Kit (Lanes 1-8). For evaluation, 10 µL of each 100 µL DNA elution was run on a 1.2 % agarose gel. Note the high yield and quality of the DNA in all lanes. Lane M: Norgen's Fast runner 1kb DNA Ladder. Figure 2. Real-time PCR Consistency from Saliva Samples. Norgen's Saliva DNA Isolation Kit was used to isolate DNA from 250 µL samples of fresh saliva. Ten samples were used from same donor. From the 100 µL elution, 5 µL of saliva DNA was directly mixed to real-time PCR master mix (total reaction volume 20 µL), and the real-time PCR reaction was performed. The GAPDH gene was successfully amplified from the different samples without any inhibition and at the same Ct, indicating the isolation consistency and the excellent quality of saliva DNA for downstream applications. Figure 3. Illumina MiSeq 16s rRNA metagenomics data from saliva samples preserved for over 6 years using Norgen's Saliva DNA Collection and Preservation Devices. The saliva DNA was isolated using Norgen's Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 6 years at room temperature. A) Principal Coordinate Analysis of 12 saliva microbiomes showing differences in the distribution of taxonomic classifications between samples up to kingdom level. B) Hierarchical clustering of 12 saliva microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram. Next Click for expanded view Kit Specifications Maximum Saliva Input 0.5 mL preserved saliva 0.25 mL fresh saliva Average Yield from 0.25 mL of Saliva* 3 - 7 μg Average Purity (OD260/280) 1.7 - 2.1 Time to Complete 10 Purifications 30 minutes * Average yield will depending on the donor Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature. Component Cat. RU45400 (50 preps) Lysis Buffer F 30 mL Proteinase K in Storage Buffer 1.2 mL Binding Buffer B 12 mL Wash Solution A 18 mL Elution Buffer B 15 mL Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (RU45400) Saliva DNA Isolation Kit - Protocol (50 prep) SUPPLEMENTARY PROTOCOLS RU45400 - Isolation of DNA from Norgen’s Swab Collection and DNA Preservation System using Norgen’s Saliva DNA Isolation Kit APPLICATION NOTES High Efficiency Real-Time PCR from Saliva DNA The Range of DNA Yield with Norgen’s Saliva DNA Collection, Preservation and Isolation Kit Determination of the DNA Molecular Weight (MW) from different Norgen Columns and Isolation Methods Stability of DNA Stored in Norgen’s Saliva DNA Preservative for 52 Months at Room Temperature Linearity of DNA Isolated from Increasing Volumes of Preserved Saliva Using Norgen’s Saliva DNA Isolation Kit DNA Isolation from Saliva Preserved with Norgen’s Saliva DNA Collection and Preservation Device using Qiagen’s QIAamp DNA Blood Comparative Study of DNA Isolated from Saliva Preserved in Norgen’s Preservative Using Norgen’s Saliva DNA Isolation Kit Versus The Effect of Elution Volume on DNA Quantity and Quality Using Norgen’s Saliva DNA Isolation Kit A Comparative Study Between Two Saliva DNA Preservation Systems, and Two Column-Based Saliva DNA Purification Methods Column-Based Isolation of DNA from Preserved Saliva Samples Offers High Quality, Consistent, and Rapid DNA Isolation Stability of DNA Stored in Norgen’s Saliva DNA Preservative at 55°C Long Term Stability of DNA Stored in Norgen’s Saliva DNA Preservative SAFETY DATA SHEETS (RU45400) Saliva DNA Isolation Kit - Safety Data Sheet POSTERS In‐Depth Analysis of the Microbial Diversity in Saliva Samples Preserved at Ambient Temperature for over 6 Years - PAG 2016 The Influence of Saliva Preservatives and DNA Isolation Methods for Oral Microbiota Metagenomics FLYERS Flyer FAQs Spin Column What to do if column gets clogged? Column clogging may occur due to one or combination of the following factors: Centrifugation speed was too low or spin time was inadequate. Check the centrifuge to ensure that it is capable of generating the required RPMs. Sufficient centrifugal force is required to move the liquid phase through the column. Also ensure that the correct spin times are followed. Spinning for a few additional minutes will help. The sample is too large. Too many cells were applied to the column. Ensure that no more than 0.5 mL of preserved saliva is applied to the column. Clogging can be alleviated by centrifuging for a longer period of time until the lysate passes through the column. The lysate/binding solution mixture is not homogeneous. To ensure a homogeneous solution, vortex for 10- 15 seconds before applying the lysate to the spin column. Why do I have a low genomic DNA yield? Low genomic DNA yield may result from one or combination of the following factors: Incomplete lysis of cells Increased. Proteinase K incubation time at 55°C may result in increased yields. The DNA elution is incomplete. Perform an additional centrifugation of 2 minutes at 14,000 x g to ensure that all the DNA is eluted. DNA concentration in the saliva sample being used is low. Some saliva samples contain very little DNA. This varies from individual to individual based on numerous variables. Increased proteinase K incubation time at 55°C may result in increased yields. Why is the purified DNA not performing well in downstream applications? Poor downstream performance of DNA may result from one or combination of the following factors: DNA was not washed with Wash Solution A. Traces of salt from the binding step may remain in the sample if the column is not washed with Wash Solution A. Salt may interfere with downstream applications, and thus must be washed from the column. Ethanol carryover. Ensure that the dry spin after the column wash steps is performed, in order to remove traces of ethanol prior to elution. Ethanol is known to interfere with many downstream applications. Why do I have RNA contamination in the DNA? RNA might get co-eluted with DNA in some cases. Carry out a digestion with RNase A on the elution if the RNA present will interfere with downstream applications. Refer to manufacturer’s instructions regarding amount of enzyme to use, optimal incubation time and temperature. Can I use Saliva DNA Isolation kit to process swab samples? Swab samples can be processed using Saliva DNA isolation kit following a supplementary proptocol. However, please note that swab samples are best processed using Norgen's Microbiome DNA isolation kit. Can I use Saliva DNA Isolation kit to process non-human saliva samples? Yes, it has been tested on canine samples, and it is expected to work on most other non-human samples. Please contact our technical support team at support@norgenbiotek.com and ask for reference publications. I lost / finished the proteinase K in storage buffer from my Norgen Biotek kit. Can I purchase it separately? Yes, please contact our technical support team at support@norgenbiotek.com for help with supply of extra proteinase K in storage buffer. Citations Title "Pursuing dynamics of minimal residual leukemic subclones in relapsed and refractory acute myeloid leukemia during conventional therapy" Journal Cancer Medicine. 2024. Authors "Dongchan Kim, Sheehyun Kim, Hyojin Song, Daehyeon Gwak, Suji Min, Ja Min Byun, Youngil Koh, Junshik Hong, Sung-Soo Yoon, Hongseok Yun, Dong-Yeop Shin Title TAS1R3 and TAS2R38 Polymorphisms Affect Sweet Taste Perception: An Observational Study on Healthy and Obese Subjects Journal Nutrients. 2022. Authors Monia Cecati, Arianna Vignini, Francesca Borroni, Sofia Pugnaloni, Sonila Alia, Jacopo Sabbatinelli, Giulia Nicolai, Marina Taus, Andrea Santarelli, Mara Fabri, Laura Mazzanti and Monica Emanuelli Title At age 9, the methylome of assisted reproductive technology children that underwent embryo culture in different media is not significantly different on a genome-wide scale Journal Human Reproduction. 2022. Authors Rebekka M Koeck, Florence Busato, Jorg Tost, Heleen Zandstra, Sylvie Remy, Sabine Langie, Marij Gielen, Ron van Golde, John C M Dumoulin, Han Brunner, Masoud Zamani Esteki, Aafke P A van Montfoort Title Functional validation of UGT1A6 in anthracycline-induced cardiotoxicity Journal UBC Theses and Dissertations. 2022. Authors Morrison, Alexander Title FOK l Vitamin D Receptor Gene Polymorphism and Risk of Dental Caries: A Case-Control Study Journal International Journal of Dentistry. 2022. Authors Nireeksha Nireeksha, Mithra N. Hegde, Shilpa S. Shetty, and Suchetha N. Kumari Title Analysis of the Prader–Willi syndrome imprinting center using droplet digital PCR and next‐generation whole‐exome sequencing Journal Molecular Genetics and Genomic Medicine. 2019. Authors Samantha N. Hartin, Waheeda A. Hossain, David Francis, David E. Godler, Sangjucta Barkataki and Merlin G. Butler Title Associations between the oxytocin receptor gene (OXTR) rs53576 polymorphism and emotional processing of social and nonsocial cues: an event-related potential (ERP) study Journal Journal of Physiological Anthropology. 2017. Authors Choi D, Minote N, Watanuki S. Title The myth of oral hygiene using synthetic mouthwash products Journal SpringerPlus. 2016. Authors Ghulam Zahara Jahangir, Durre Shahwar Ashraf, Idrees Ahmad Nasir, Madeha Sadiq, Sobia Shahzad, Farah Naz, Muhammad Iqbal and Afifa Saeed Title Case-control study of glucocorticoid receptor and corticotrophin-releasing hormone receptor gene variants and risk of perinatal depression Journal BMC Pregnancy and Childbirth. 2015. Authors Ene-Choo Tan, Tze-Ern Chua, Theresa M. Y. Lee, Hui-San Tan, Joe L. Y. Ting and Helen Y. Chen Title First Detection of Antibodies Against African Swine Fever Virus in Faeces Samples Journal Transboundary and Emerging Diseases. 2015. Authors E. Nieto-Pelegrın, B. Rivera-Arroyo and J. M. Sanchez-Vizcaıno Title 1000 Norms Project: protocol of a cross-sectional study cataloging human variation Journal Physiotherapy. 2015. Authors Marnee J. McKay, Jennifer N. Baldwin, Paulo Ferreira, Milena Simic, Natalie Vanicek, Claire E. Hiller, Elizabeth J. Nightingale, Niamh A. Moloney, Kate G. Quinlan, Fereshteh Pourkazemi, Amy D. Sman, Leslie L. Nicholson, Seyed J. Mousavi, Kristy Rose, Jacqueline Raymond, Martin G. Mackey, Angus Chard, Markus Hübscher, Caleb Wegener, Alycia Fong Yan, Kathryn M. Refshauge, Joshua Burns Title TAS2R38 single nucleotide polymorphisms are associated with PROP- but not thermal tasting: a pilot study. Journal Chemosensory Perception. 2013. Authors Bering A, Pickering G, Liang P. Title Brief communication: Evolution of a specific O allele (O1v(G542A) ) supports unique ancestry of Native Americans. Journal American Journal of Physical Anthropology. 2013. Authors Villanea FA, Bolnick DA, Monroe C, Worl R, Cambra R, Leventhal A, Kemp BM. Title Differences in the quantity of DNA found in the urine and saliva of smokers versus nonsmokers: implications for the timing of epigenetic events. Journal Epigenomics. 2012. Authors Simkin M, Abdalla M, El-Mogy M, Haj-Ahmad Y. Related Products Saliva DNA Isolation Kit (Magnetic Bead System) Saliva DNA Collection and Preservation Devices Saliva DNA Isolation 96-Well Kit
Saliva DNA Isolation Kit For the rapid purification of high-quality DNA from preserved and fresh saliva samples For research use only and NOT intended for in vitro diagnostics.Diagnostic version available here Powered by Bioz Saliva DNA Isolation Kit For the rapid purification of high-quality DNA from preserved and fresh saliva samples Price USD$169.00 SKU RU45400 Format Spin Column Size 50 Preps Quantity
