Cryptosporidium TaqMan RT-PCR Detection Kits

Features and Benefits

Detection kits for Cryptosporidium
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis

Cryptosporidium is a parasite found in water that causes an infection in mammals termed cryptosporidiosis. It is one of the most common water-borne diseases and is found world-wide. It affects the intestines of mammals and typically causes an acute short-term infection. The most common symptom is self-limiting diarrhea in healthy individuals, however in immunocompromised individuals the symptoms are particularly severe and often fatal. There is no specific treatment for cryptosporidiosis other than fluid rehydration and management of any pain. Therefore early detection of Cryptosporidium in water is the foremost action to prevent the infection.

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Cryptosporidium TaqMan RT-PCR Kit, 100 reactions

Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents

Cryptosporidium TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

Details

Supporting Data

TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.

Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM39150 (100 preps)	Cat. TM39110 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	-
Cryptosporidium Primer & Probe Mix	280 μL	280 μL
Cryptosporidium Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Documentation

PROTOCOLS

(TM39150) Cryptosporidium TaqMan RT-PCR Kit - Protocol (100 rxns)

(TM39110) Cryptosporidium TaqMan Probe/Primer and Control Set - Protocol (100 rxns)

SAFETY DATA SHEETS

TM39150 - Cryptosporidium TaqMan RT-PCR Detection Kit - SDS

TM39110 - Cryptosporidium Taqman RT-PCR Detection Kit - SDS

Citations

Title	Assessment of the potential occurrence of Cryptosporidium species in various water sources in Sharqia Governorate, Egypt
Citation	Journal of Parasitic Diseases 2024.
Authors	Marwa Omar, Samia E. Etewa, Samar A. M. Mahmoud & Tahani I. Farag

Cryptosporidium TaqMan RT-PCR Detection Kits

Cryptosporidium TaqMan RT-PCR Detection Kits

Features and Benefits