For purification of total RNA (including microRNA) from all types of tissues including fiber-rich tissues

  • Extract high quality & purity total RNA including miRNA 
  • Isolate from a wide variety of animal tissues 
  • No phenol step required - isolate all RNA in one fraction
  • Compatible with tissues stored in RNAlater® or Trizol®  
  • Convenient & fast spin column format

ItemsCat. #Size
Animal Tissue RNA Purification Kit 25700 50 preps

Animal Tissue RNA Purification Kit

This kit is suitable for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, intestine, etc.  Briefly, the tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins and connective tissues. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Kit Specifications
Maximum Column Binding Capacity
 50 μg 
Maximum Column Loading Volume
650 μL
Size of RNA Purified
All sizes, including small RNA < 200 nt 
Time to Complete 10 Purifications
50 minutes
Average Yield
Liver (10mg)
Kidney (10mg)
Spleen (10mg)
Heart (10mg)
Muscle (10mg)
30 μg
25 μg
15 μg
10 μg
5 μg
Maximum Amount of Starting Material   
30 mg
15 mg
15 mg
30 mg
15 mg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. These reagents should remain stable for at least 1 year in their unopened containers.

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Title NGF and P75NTR Gene Expression Is Associated with the Hepatic Fibrosis Stage Due to Viral and Non-Viral Causes
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Authors Ednelza da Silva Graça Amoras, Samara Tatielle Monteiro Gomes, Felipe Bonfim Freitas, Bárbara Brasil Santana, Geraldo Ishak, Marialva Tereza Ferreira de Araújo, Sâmia Demachki, Simone Regina Souza da Silva Conde, Marluísa de Oliveira Guimarães Ishak, Ricardo Ishak, Antonio Carlos Rosário Vallinoto
Title TBCRC 019: phase II trial of nab-PAC with/without the anti-death receptor 5 monoclonal antibody tigatuzumab in patients with triple negative breast cancer
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Title miR-23a is decreased during muscle atrophy by a mechanism that includes calcineurin signaling and exosome-mediated export.
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Title Pharmacological ceramide reduction alleviates alcohol-induced steatosis and hepatomegaly in adiponectin knockout mice.
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Title The analysis of eight transcriptomes from all Porifera classes reveals surprising genetic complexity in sponges.
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Title TLR dependent XBP-1 activation induces an autocrine loop in rheumatoid arthritis synoviocytes.
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Title Differential Expression Profiling and Functional Analysis of microRNAs through Stage I-III Papillary Thyroid Carcinoma.
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Authors Ince S, Keles H, Erdogan M, Hazman O, Kucukkurt I.
Title Expression profile of microRNAs in rat hippocampus following lithium-pilocarpine-induced status epilepticus.
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Authors Hu K, Zhang C, Long L, Long X, Feng L, Li Y, Xiao B.
Title Correlation and quantitation of microRNA aberrant expression in tissues and sera from patients with breast tumor.
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