For purification of total RNA (including microRNA) from all types of tissues including fiber-rich tissues

  • Extract high quality & purity total RNA including miRNA
  • Isolate from a wide variety of animal tissues
  • No phenol step required - isolate all RNA in one fraction
  • Compatible with tissues stored in RNAlater® or Trizol®
  • Convenient & fast spin column format

ItemsCat. #Size
Animal Tissue RNA Purification Kit 25700 50 preps

Animal Tissue RNA Purification Kit

This kit is suitable for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, intestine, etc. Briefly, the tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins and connective tissues. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Kit Specifications
Column Binding Capacity
Up to 50 μg RNA
Maximum Column Loading Volume
650 μL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 10 Purifications
50 minutes
RNA Yield
Liver (10mg)
Kidney (10mg)
Spleen (10mg)
Heart (10mg)
Muscle (10mg)
30 μg
25 μg
15 μg
10 μg
5 μg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 25700 (50 preps)
Buffer RL 30 mL
RNase-Free Water 40 mL
Wash Solution A 38 mL
Enzyme Incubation Buffer A 6 mL
Elution Solution A 6 mL
Proteinase K 2 vials
DNase I 1 vial
Mini Spin Columns 50
Collection Tubes 100
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Whole Transcriptome of the Venom Gland from Urodacus yaschenkoi Scorpion
Journal PLOS ONE. 2015.
Authors Karen Luna-Ramírez, Verónica Quintero-Hernández, Víctor Rivelino Juárez-González, Lourival D. Possani
Title Transcript profiles in longissimus dorsi muscle and subcutaneous adipose tissue: A comparison of pigs with different postweaning growth rates
Journal Journal of Animal Science. 2015.
Authors C. M. Pilcher, C. K. Jones, M. Schroyen, A. J. Severin, J. F. Patience, C. K. Tuggle and J. E. Koltes
Title Knockdown of microRNA-1323 restores sensitivity to radiation by suppression of PRKDC activity in radiation-resistant lung cancer cells
Journal Oncology Reports. 2015.
Authors Yong Li, Wei Han, Ting-Ting Ni, Liang Lu, Min Huang, Yu Zhang, Hui Cao, Han-Qun Zhang, Wen Luo, Hang Li
Title NGF and P75NTR Gene Expression Is Associated with the Hepatic Fibrosis Stage Due to Viral and Non-Viral Causes
Journal PLOS ONE. 2015.
Authors Ednelza da Silva Graça Amoras, Samara Tatielle Monteiro Gomes, Felipe Bonfim Freitas, Bárbara Brasil Santana, Geraldo Ishak, Marialva Tereza Ferreira de Araújo, Sâmia Demachki, Simone Regina Souza da Silva Conde, Marluísa de Oliveira Guimarães Ishak, Ricardo Ishak, Antonio Carlos Rosário Vallinoto
Title TBCRC 019: phase II trial of nab-PAC with/without the anti-death receptor 5 monoclonal antibody tigatuzumab in patients with triple negative breast cancer
Journal Clinical Cancer Research. 2015.
Authors Andres Forero-Torres, Katherine E. Varley, Vandana Gupta Abramson, Yufeng Li, Christos Vaklavas, Nancy U. Lin, Minetta C. Liu, Hope S. Rugo, Rita Nanda, Anna Maria Storniolo, Tiffany A. Traina, Sujata Pati, Catherine H. Van Poznak, Julie R. Nangia, William Johnson Irvin Jr., Helen Krontiras, Jennifer F. De Los Santos, Paul Haluska, William Grizzle, Richard M. Meyers2, Antonio C. Wolff
Title Genome-wide mRNA and miRNA expression profiling reveal multiple regulatory networks in colorectal cancer.
Journal Cell Death and Disease.. 2015.
Authors R Vishnubalaji, R Hamam, M-H Abdulla, M A V Mohammed, M Kassem, O Al-Obeed, A Aldahmash and N M Alajez.
Title Perinatal Administration of a Bitter Tastant Influences Gene Expression in Chicken Palate and Duodenum.
Journal Journal of Agriculture and Food Chemistry.. 2014.
Authors Shira L. Cheled-Shoval , Maik Behrens , Wolfgang Meyerhof , Masha Y. Niv , and Zehava Uni.
Title Cigarette smoke affects dendritic cell maturation in the small airways of patients with chronic obstructive pulmonary disease.
Journal Molecular Medicine Reports.. 2014.
Authors Shi‑Xia Liao, Ting Ding, Xi‑Min Rao, De‑Sheng Sun, Peng‑Peng Sun, Ya‑Jun Wang, Dan‑Dan Fu, Xiao‑Li Liu, Yao Ou‑Yang.
Title Effect of brachytherapy on NF-κB and VEGF in gastric carcinoma xenografts.
Journal Oncology Reports.. 2014.
Authors Wan-Fu Zhang, Wen-Di Jin, Bo Li, Ming-Chun Wang, Xiao-Gang Li, Wen-Yuan Mao, Kai-Yuan Luo.
Title Novel interactive effects of darkness and retinoid signaling in the ability to form long-term memory following aversive operant conditioning.
Journal Neurobiology of Learning and Memory.. 2014.
Authors Cailin M. Rothwell, Jason Simmons, Grace Peters, Gaynor E. Spencer.
Title miR-23a is decreased during muscle atrophy by a mechanism that includes calcineurin signaling and exosome-mediated export.
Journal American Journal of Physiology. Cell Physiology.. 2014.
Authors Hudson MB, Woodworth-Hobbs ME, Zheng B, Rahnert JA, Blount MA, Gooch JL, Searles CD, Price SR.
Title Recurrent read-through fusion transcripts in breast cancer.
Journal Breast Cancer Research and Treatment. 2014.
Authors Katherine E. Varley, Jason Gertz, Brian S. Roberts, Nicholas S. Davis, Kevin M. Bowling, Marie K. Kirby, Amy S. Nesmith, Patsy G. Oliver, William E. Grizzle, Andres Forero, Donald J. Buchsbaum, Albert F. LoBuglio, Richard M. Myers.
Title Pharmacological ceramide reduction alleviates alcohol-induced steatosis and hepatomegaly in adiponectin knockout mice.
Journal Archives of Virology. 2014.
Authors Correnti JM, Juskeviciute E, Swarup A, Hoek JB.
Title The analysis of eight transcriptomes from all Porifera classes reveals surprising genetic complexity in sponges.
Journal Molecular Biology and Evolution. 2014.
Authors Riesgo A, Farrar N, Windsor PJ, Giribet G, Leys SP.
Title TLR dependent XBP-1 activation induces an autocrine loop in rheumatoid arthritis synoviocytes.
Journal Journal of Autoimmunology. 2013.
Authors Savic S, Ouboussad L, Dickie LJ, Geiler J, Wong C, Doody GM, Churchman SM, Ponchel F, Emery P, Cook GP, Buch MH, Tooze RM, McDermott MF.
Title Hypothalamic neuropeptide Y (NPY) gene expression is not affected by central serotonin in the rainbow trout (Oncorhynchus mykiss).
Journal Comparative Biochemistry and Physiology. 2013.
Authors Mancebo MJ, Ceballos FC, Pérez-Maceira J, Aldegunde M.
Title Differential Expression Profiling and Functional Analysis of microRNAs through Stage I-III Papillary Thyroid Carcinoma.
Journal International Journal of Medical Sciences. 2013.
Authors Zhang J, Liu Y, Liu Z, Wang XM, Yin DT, Zheng LL, Zhang DY, Lu XB.
Title Occult hepatitis C infection: the prevalence and profile of its immunoregulatory cytokines.
Journal GEgyptian Liver Journal. 2012.
Authors Gad Y, Ahmad N, Mousa N, Farag R, Abdel-Aziz A, Abousmra N, Elhadidy M.
Title Transposase mediated construction of RNA-seq libraries.
Journal Genome Research. 2011.
Authors Gertz J, Varley KE, Davis NS, Baas BJ, Goryshin IY, Vaidyanathan R, Kuersten S, Myers RM.
Title Protective effect of boric acid against carbon tetrachloride-induced hepatotoxicity in mice.
Journal Drug and Chemical Toxicology. 2011.
Authors Ince S, Keles H, Erdogan M, Hazman O, Kucukkurt I.
Title Expression profile of microRNAs in rat hippocampus following lithium-pilocarpine-induced status epilepticus.
Journal Neuroscience Letters. 2010.
Authors Hu K, Zhang C, Long L, Long X, Feng L, Li Y, Xiao B.
Title Correlation and quantitation of microRNA aberrant expression in tissues and sera from patients with breast tumor.
Journal Geonocologic Oncology. 2010.
Authors Wang F, Zheng Z, Guo J, Ding X.