|Cell Culture Media Exosome Purification Mini Kit||60400||50 preps|
|Cell Culture Media Exosome Purification Midi Kit||60500||25 preps|
|Cell Culture Media Exosome Purification Maxi Kit||60600||15 preps|
Cell Culture Media Exosome Purification Kits
The Cell Culture Media Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different cell culture media sample volumes ranging from 5 mL to 35 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different cell culture media sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen's proprietary resin.
These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.
Exosomes enriched with Norgen's Cell Culture Media Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration
Exosomal RNA can be isolated using Norgen's Exosomal RNA Isolation Kit from exosomes enriched using Norgen's Cell Culture Media Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for biomarker discovery. Exosomal RNA isolation is based on Norgen's proprietary resin without the need for phenol extractions or carrier RNA.
Exosomes isolated from cell culture media at Norgen
|Cell Culture Media Input (Cat. 60400)||
5 mL - 10 mL
Cell Culture Media Input (Cat. 60500)
10 mL - 20 mL
|Cell Culture Media Input (Cat. 60600)||20 mL - 35 mL|
Size of Exosomes Purified
40 nm - 150 nm
|Elution Volume||50 - 100 µL|
|Time to Complete 10 Purifications||
30 - 45 minutes
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.
|Title||BK Polyomavirus MicroRNA Levels in Exosomes Are Modulated by Non-Coding Control Region Activity and Down-Regulate Viral Replication When Delivered to Non-Infected Cells Prior to Infection|
|Journal||Viruses. September 2018.|
|Authors||Francesco Martelli, Zongsong Wu, Serena Delbue, Fabian H. Weissbach, Maria Chiara Giulioli, Pasquale Ferrante, Hans H. Hirsch, and Simone Giannecchini|
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