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Cell Culture Media Exosome Purification Kits (Cat. 60400, 60500, 60600)

 

Purchasing Information

  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Versatile sample input ranging from 5 mL to 35 mL
    • Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL)
    • Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL)
    • Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL)
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents or overnight incubation required
  • No protease treatment required
  • Compatible with cell culture media from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration

Related Services


NGS

RNA Isolation

ItemsCat. #Size
Cell Culture Media Exosome Purification Mini Kit 60400 50 preps
Cell Culture Media Exosome Purification Midi Kit 60500 25 preps
Cell Culture Media Exosome Purification Maxi Kit 60600 15 preps

  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Versatile sample input ranging from 5 mL to 35 mL
    • Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL)
    • Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL)
    • Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL)
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents or overnight incubation required
  • No protease treatment required
  • Compatible with cell culture media from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration

Related Services


NGS

RNA Isolation


Cell Culture Media Exosome Purification Kits

The Cell Culture Media Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different cell culture media sample volumes ranging from 5 mL to 35 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different cell culture media sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen's proprietary resin.

These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.

NanoSight® Analysis

Exosomes enriched with Norgen's Cell Culture Media Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration

Exosomal RNA Analysis

Exosomal RNA can be isolated using Norgen's Exosomal RNA Isolation Kit from exosomes enriched using Norgen's Cell Culture Media Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for biomarker discovery. Exosomal RNA isolation is based on Norgen's proprietary resin without the need for phenol extractions or carrier RNA.

Exosomes isolated from cell culture media at Norgen

Kit Specifications
Cell Culture Media Input (Cat. 60400)
5 mL - 10 mL
Cell Culture Media Input (Cat. 60500)
10 mL - 20 mL
Cell Culture Media Input (Cat. 60600) 20 mL - 35 mL
Size of Exosomes Purified
40 nm - 150 nm
Elution Volume 50 - 100 µL
Time to Complete 10 Purifications
30 - 45 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.


Title BK Polyomavirus MicroRNA Levels in Exosomes Are Modulated by Non-Coding Control Region Activity and Down-Regulate Viral Replication When Delivered to Non-Infected Cells Prior to Infection
Journal Viruses. September 2018.
Authors Francesco Martelli, Zongsong Wu, Serena Delbue, Fabian H. Weissbach, Maria Chiara Giulioli, Pasquale Ferrante, Hans H. Hirsch, and Simone Giannecchini

ItemsCat. #Size
Cell Culture Media Exosome Purification Mini Kit 60400 50 preps
Cell Culture Media Exosome Purification Midi Kit 60500 25 preps
Cell Culture Media Exosome Purification Maxi Kit 60600 15 preps

  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Versatile sample input ranging from 5 mL to 35 mL
    • Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL)
    • Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL)
    • Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL)
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents or overnight incubation required
  • No protease treatment required
  • Compatible with cell culture media from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration

Related Services


NGS

RNA Isolation


Cell Culture Media Exosome Purification Kits

The Cell Culture Media Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different cell culture media sample volumes ranging from 5 mL to 35 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different cell culture media sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen's proprietary resin.

These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.

NanoSight® Analysis

Exosomes enriched with Norgen's Cell Culture Media Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration

Exosomal RNA Analysis

Exosomal RNA can be isolated using Norgen's Exosomal RNA Isolation Kit from exosomes enriched using Norgen's Cell Culture Media Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for biomarker discovery. Exosomal RNA isolation is based on Norgen's proprietary resin without the need for phenol extractions or carrier RNA.

Exosomes isolated from cell culture media at Norgen

Kit Specifications
Cell Culture Media Input (Cat. 60400)
5 mL - 10 mL
Cell Culture Media Input (Cat. 60500)
10 mL - 20 mL
Cell Culture Media Input (Cat. 60600) 20 mL - 35 mL
Size of Exosomes Purified
40 nm - 150 nm
Elution Volume 50 - 100 µL
Time to Complete 10 Purifications
30 - 45 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Title BK Polyomavirus MicroRNA Levels in Exosomes Are Modulated by Non-Coding Control Region Activity and Down-Regulate Viral Replication When Delivered to Non-Infected Cells Prior to Infection
Journal Viruses. September 2018.
Authors Francesco Martelli, Zongsong Wu, Serena Delbue, Fabian H. Weissbach, Maria Chiara Giulioli, Pasquale Ferrante, Hans H. Hirsch, and Simone Giannecchini

 

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