Cell Culture Media Exosome Purification Kits All-in-one system for the purification of cell culture media exosomes For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Cell Culture Media Exosome Purification Kits All-in-one system for the purification of cell culture media exosomes SKU 60400 Format Mini Maxi Size 50 Preps Contact Distributor Overview Details Documentation Citations Overview Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes. Versatile sample input ranging from 5 mL to 35 mL Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL) Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL) Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins. No precipitation reagents, overnight incubation, protease or coagulant treatments required No time-consuming ultracentrifugation, filtration or special syringes required Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type Purified exosomes are compatible with functional studies. Purified exosomes are free from any protein-bound cell-free circulating RNA Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration. Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method. The Cell Culture Media Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different cell culture media sample volumes ranging from 5 mL to 35 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different cell culture media sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen's proprietary resin. These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression. NanoSight® Analysis Exosomes enriched with Norgen's Cell Culture Media Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration Exosomal RNA Analysis Exosomal RNA can be isolated using Norgen's Exosomal RNA Isolation Kit from exosomes enriched using Norgen's Cell Culture Media Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for biomarker discovery. Exosomal RNA isolation is based on Norgen's proprietary resin without the need for phenol extractions or carrier RNA. Exosomes isolated from cell culture media at Norgen Details Supporting Data Previous Figure 1. Isolation of RNA from exosomes purified from different cell culture media volumes. Norgen's Cell Culture Media Exosome Purification Mini Kit (Cat. 60400) was used to isolate exosomal RNA from exosomes purified from different cell culture media volumes using the same kit. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated exosomal RNA (A) The exosomal miR-26a is linearly decreasing with increasing the sample input volume. (B) The relative amount of the exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90% Figure 2. Isolation of RNA from exosomes purified from different cell culture media volumes. Norgen's Cell Culture Media Exosome Purification Maxi Kit (Cat. 60600) was used to isolate exosomal RNA from different cell culture media volumes from exosomes purified using the same kit. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated exosomal RNA. (A) The exosomal miR-26a is linearly decreasing with increasing the sample input volume. (B) The relative amount of the exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%. Next Click for expanded view Purification and enrichment of intact cell culture media exosomes for functional studies Versatile sample input ranging from 5 mL to 35 mL Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL) Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL) Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL) No time-consuming ultracentrifugation, filtration nor special syringes required No precipitation reagents or overnight incubation required No protease treatment required Compatible with cell culture media from any species Pure exosomes are purified and are free-from any other RNA-binding proteins Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration Purification is based on Norgen’s proprietary Silicon Carbide resin matrix Storage Conditions All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 60400 (50 preps) Cat. 60600 (15 preps) Slurry E 12.5 mL 12.5 mL ExoC Buffer 1.5 mL 1.5 mL ExoR Buffer 12 mL 12 mL Mini Filter Spin Columns inserted into 2 mL tubes 50 15 Product Insert 1 1 Documentation PROTOCOLS (60400) Cell Culture Media Exosome Purification Kits - Protocol (50 prep) (60600) Cell Culture Media Exosome Purification Kits - Protocol (15 prep) SAFETY DATA SHEETS (60400, 60500, 60600) Cell Culture Media Exosome Purification Kits - Safety Data Sheets RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Title Adipose-Derived Mesenchymal Stem Cell-Derived Extracellular Vesicles Rescue Tendon Injury in Rat via the miR-19 a/IGFBP3 Axis Journal Stem Cells International. 2022. Authors Haibo Zhao, Hongyuan Jiang, Haoyun Zhang, Zewen Sun, Qian Lin, Tianrui Wang ,Tengbo Yu and Yingze Zhang Title Comparative Proteomic Profiling of Secreted Extracellular Vesicles from Breast Fibroadenoma and Malignant Lesions: A Pilot Study Journal Int. J. Mol. Sci.. 2022. Authors Katia Pane, Cristina Quintavalle, Silvia Nuzzo, Francesco Ingenito, Giuseppina Roscigno, Alessandra Affinito, Iolanda Scognamiglio, Birlipta Pattanayak, Enrico Gallo, Antonella Accardo, Guglielmo Thomas, Zoran Minic, Maxim V. Berezovski, Monica Franzese, and Gerolama Condorelli Title Vitamin D Reverts the Exosome-Mediated Transfer of Cancer Resistance to the mTOR Inhibitor Everolimus in Hepatocellular Carcinoma Journal Front Oncol. 2022. Authors Mariarosaria Negri, Feliciana Amatrudo, Annalisa Gentile, Roberta Patalano, Tatiana Montò, Cristina de Angelis, Chiara Simeoli, Rosa Pirchio, Renata Simona Auriemma, Annamaria Colao, Rosario Pivonello, and Claudia Pivonello Title Comparative Proteomic Profiling of Secreted Extracellular Vesicles from Breast Fibroadenoma and Malignant Lesions: A Pilot Study Journal Int. J. Mol. Sci. April 2022. Authors Pane K., Quintavalle C., Nuzzo S., Ingenito F., Roscigno G., Affinito A., Scognamiglio I., Pattanayak B., Gallo E., Accardo A. and Thomas G., Title Small RNA sequencing and bioinformatics analysis of RAW264.7-derived exosomes after Mycobacterium Bovis Bacillus Calmette-Guérin infection Journal BMC Genomics. May 2022. Authors Zhan, Xuehua, Wenqi Yuan, Yueyong Zhou, Rong Ma, and Zhaohui Ge. Title Microarray analysis of circRNAs sequencing profile in exosomes derived from bone marrow mesenchymal stem cells in postmenopausal osteoporosis patients Journal JCLA. 2021. Authors Miao Fu,Liping Fang,Xi Xiang,Xijing Fan,Junqi Wu,Jinhua Wang Title Protective role of mesenchymal stem cells and mesenchymal stem cell-derived exosomes in cigarette smoke-induced mitochondrial dysfunction in mice Journal Toxicol Appl Pharmacol. 2019. Authors Krishna Prahlad Maremanda, Isaac K. Sundar, and Irfan Rahman* Title BK Polyomavirus MicroRNA Levels in Exosomes Are Modulated by Non-Coding Control Region Activity and Down-Regulate Viral Replication When Delivered to Non-Infected Cells Prior to Infection Journal Viruses. September 2018. Authors Francesco Martelli, Zongsong Wu, Serena Delbue, Fabian H. Weissbach, Maria Chiara Giulioli, Pasquale Ferrante, Hans H. Hirsch, and Simone Giannecchini Related Services NGS RNA Isolation Related Products Exosomal RNA Isolation Kit
Cell Culture Media Exosome Purification Kits All-in-one system for the purification of cell culture media exosomes For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Cell Culture Media Exosome Purification Kits All-in-one system for the purification of cell culture media exosomes SKU 60400 Format Mini Maxi Size 50 Preps Contact Distributor