Cell Culture Media Exosome Purification Kits (Cat. 60400, 60500, 60600)

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Cell Culture Media Exosome Purification Kits

Cat. 60400, 60500, 60600
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Cell Culture Media Exosome Purification Kits

Cat. 60400, 60500, 60600

All-in-one system for the purification of cell culture media exosomes

  • Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
  • Versatile sample input ranging from 5 mL to 35 mL
    • Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL)
    • Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL)
    • Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL)
  • Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.
  • No precipitation reagents, overnight incubation, protease or coagulant treatments required
  • No time-consuming ultracentrifugation, filtration or special syringes required
  • Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
  • Purified exosomes are compatible with functional studies.
  • Purified exosomes are free from any protein-bound cell-free circulating RNA
  • Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
  • Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method.

For research use only and NOT intended for in vitro diagnostics.

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Cell Culture Media Exosome Purification Mini Kit
(Cat. 60400) 50 preps
Cell Culture Media Exosome Purification Midi Kit
(Cat. 60500) 25 preps
Cell Culture Media Exosome Purification Maxi Kit
(Cat. 60600) 15 preps

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. 60400
  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Versatile sample input ranging from 5 mL to 35 mL
    • Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL)
    • Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL)
    • Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL)
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents or overnight incubation required
  • No protease treatment required
  • Compatible with cell culture media from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
  • Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

Storage Conditions

All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 60500
  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Versatile sample input ranging from 5 mL to 35 mL
    • Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL)
    • Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL)
    • Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL)
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents or overnight incubation required
  • No protease treatment required
  • Compatible with cell culture media from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
  • Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

Storage Conditions

All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 60600
  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Versatile sample input ranging from 5 mL to 35 mL
    • Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL)
    • Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL)
    • Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL)
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents or overnight incubation required
  • No protease treatment required
  • Compatible with cell culture media from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
  • Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

Storage Conditions

All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Click for expanded view

Title Adipose-Derived Mesenchymal Stem Cell-Derived Extracellular Vesicles Rescue Tendon Injury in Rat via the miR-19 a/IGFBP3 Axis
Journal Stem Cells International. 2022.
Authors Haibo Zhao, Hongyuan Jiang, Haoyun Zhang, Zewen Sun, Qian Lin, Tianrui Wang ,Tengbo Yu and Yingze Zhang
Title Comparative Proteomic Profiling of Secreted Extracellular Vesicles from Breast Fibroadenoma and Malignant Lesions: A Pilot Study
Journal Int. J. Mol. Sci.. 2022.
Authors Katia Pane, Cristina Quintavalle, Silvia Nuzzo, Francesco Ingenito, Giuseppina Roscigno, Alessandra Affinito, Iolanda Scognamiglio, Birlipta Pattanayak, Enrico Gallo, Antonella Accardo, Guglielmo Thomas, Zoran Minic, Maxim V. Berezovski, Monica Franzese, and Gerolama Condorelli
Title Vitamin D Reverts the Exosome-Mediated Transfer of Cancer Resistance to the mTOR Inhibitor Everolimus in Hepatocellular Carcinoma
Journal Front Oncol. 2022.
Authors Mariarosaria Negri, Feliciana Amatrudo, Annalisa Gentile, Roberta Patalano, Tatiana Montò, Cristina de Angelis, Chiara Simeoli, Rosa Pirchio, Renata Simona Auriemma, Annamaria Colao, Rosario Pivonello, and Claudia Pivonello
Title Comparative Proteomic Profiling of Secreted Extracellular Vesicles from Breast Fibroadenoma and Malignant Lesions: A Pilot Study
Journal Int. J. Mol. Sci. April 2022.
Authors Pane K., Quintavalle C., Nuzzo S., Ingenito F., Roscigno G., Affinito A., Scognamiglio I., Pattanayak B., Gallo E., Accardo A. and Thomas G.,
Title Small RNA sequencing and bioinformatics analysis of RAW264.7-derived exosomes after Mycobacterium Bovis Bacillus Calmette-Guérin infection
Journal BMC Genomics. May 2022.
Authors Zhan, Xuehua, Wenqi Yuan, Yueyong Zhou, Rong Ma, and Zhaohui Ge.
Title Protective role of mesenchymal stem cells and mesenchymal stem cell-derived exosomes in cigarette smoke-induced mitochondrial dysfunction in mice
Journal Toxicol Appl Pharmacol. 2019.
Authors Krishna Prahlad Maremanda, Isaac K. Sundar, and Irfan Rahman*
Title BK Polyomavirus MicroRNA Levels in Exosomes Are Modulated by Non-Coding Control Region Activity and Down-Regulate Viral Replication When Delivered to Non-Infected Cells Prior to Infection
Journal Viruses. September 2018.
Authors Francesco Martelli, Zongsong Wu, Serena Delbue, Fabian H. Weissbach, Maria Chiara Giulioli, Pasquale Ferrante, Hans H. Hirsch, and Simone Giannecchini

Cell Culture Media Exosome Purification Kits

The Cell Culture Media Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different cell culture media sample volumes ranging from 5 mL to 35 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different cell culture media sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen's proprietary resin.

These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.

NanoSight® Analysis

Exosomes enriched with Norgen's Cell Culture Media Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration

Exosomal RNA Analysis

Exosomal RNA can be isolated using Norgen's Exosomal RNA Isolation Kit from exosomes enriched using Norgen's Cell Culture Media Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for biomarker discovery. Exosomal RNA isolation is based on Norgen's proprietary resin without the need for phenol extractions or carrier RNA.

Exosomes isolated from cell culture media at Norgen

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 60400
  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Versatile sample input ranging from 5 mL to 35 mL
    • Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL)
    • Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL)
    • Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL)
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents or overnight incubation required
  • No protease treatment required
  • Compatible with cell culture media from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
  • Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

Storage Conditions

All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 60500
  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Versatile sample input ranging from 5 mL to 35 mL
    • Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL)
    • Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL)
    • Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL)
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents or overnight incubation required
  • No protease treatment required
  • Compatible with cell culture media from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
  • Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

Storage Conditions

All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 60600
  • Purification and enrichment of intact cell culture media exosomes for functional studies
  • Versatile sample input ranging from 5 mL to 35 mL
    • Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL)
    • Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL)
    • Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL)
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents or overnight incubation required
  • No protease treatment required
  • Compatible with cell culture media from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
  • Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

Storage Conditions

All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Title Adipose-Derived Mesenchymal Stem Cell-Derived Extracellular Vesicles Rescue Tendon Injury in Rat via the miR-19 a/IGFBP3 Axis
Journal Stem Cells International. 2022.
Authors Haibo Zhao, Hongyuan Jiang, Haoyun Zhang, Zewen Sun, Qian Lin, Tianrui Wang ,Tengbo Yu and Yingze Zhang
Title Comparative Proteomic Profiling of Secreted Extracellular Vesicles from Breast Fibroadenoma and Malignant Lesions: A Pilot Study
Journal Int. J. Mol. Sci.. 2022.
Authors Katia Pane, Cristina Quintavalle, Silvia Nuzzo, Francesco Ingenito, Giuseppina Roscigno, Alessandra Affinito, Iolanda Scognamiglio, Birlipta Pattanayak, Enrico Gallo, Antonella Accardo, Guglielmo Thomas, Zoran Minic, Maxim V. Berezovski, Monica Franzese, and Gerolama Condorelli
Title Vitamin D Reverts the Exosome-Mediated Transfer of Cancer Resistance to the mTOR Inhibitor Everolimus in Hepatocellular Carcinoma
Journal Front Oncol. 2022.
Authors Mariarosaria Negri, Feliciana Amatrudo, Annalisa Gentile, Roberta Patalano, Tatiana Montò, Cristina de Angelis, Chiara Simeoli, Rosa Pirchio, Renata Simona Auriemma, Annamaria Colao, Rosario Pivonello, and Claudia Pivonello
Title Comparative Proteomic Profiling of Secreted Extracellular Vesicles from Breast Fibroadenoma and Malignant Lesions: A Pilot Study
Journal Int. J. Mol. Sci. April 2022.
Authors Pane K., Quintavalle C., Nuzzo S., Ingenito F., Roscigno G., Affinito A., Scognamiglio I., Pattanayak B., Gallo E., Accardo A. and Thomas G.,
Title Small RNA sequencing and bioinformatics analysis of RAW264.7-derived exosomes after Mycobacterium Bovis Bacillus Calmette-Guérin infection
Journal BMC Genomics. May 2022.
Authors Zhan, Xuehua, Wenqi Yuan, Yueyong Zhou, Rong Ma, and Zhaohui Ge.
Title Protective role of mesenchymal stem cells and mesenchymal stem cell-derived exosomes in cigarette smoke-induced mitochondrial dysfunction in mice
Journal Toxicol Appl Pharmacol. 2019.
Authors Krishna Prahlad Maremanda, Isaac K. Sundar, and Irfan Rahman*
Title BK Polyomavirus MicroRNA Levels in Exosomes Are Modulated by Non-Coding Control Region Activity and Down-Regulate Viral Replication When Delivered to Non-Infected Cells Prior to Infection
Journal Viruses. September 2018.
Authors Francesco Martelli, Zongsong Wu, Serena Delbue, Fabian H. Weissbach, Maria Chiara Giulioli, Pasquale Ferrante, Hans H. Hirsch, and Simone Giannecchini

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