Cell Culture Media Exosome Purification Kits (Cat. 60400, 60500, 60600)

Purification and enrichment of intact cell culture media exosomes for functional studies
Versatile sample input ranging from 5 mL to 35 mL
- Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL)
- Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL)
- Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL)
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents or overnight incubation required
No protease treatment required
Compatible with cell culture media from any species
Pure exosomes are purified and are free-from any other RNA-binding proteins
Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration

Related Services

Items	Cat. #	Size
Cell Culture Media Exosome Purification Mini Kit	60400	50 preps
Cell Culture Media Exosome Purification Midi Kit	60500	25 preps
Cell Culture Media Exosome Purification Maxi Kit	60600	15 preps

Cell Culture Media Exosome Purification Kits

The Cell Culture Media Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different cell culture media sample volumes ranging from 5 mL to 35 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different cell culture media sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen's proprietary resin.

These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.

NanoSight® Analysis

Exosomes enriched with Norgen's Cell Culture Media Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration

Exosomal RNA Analysis

Exosomal RNA can be isolated using Norgen's Exosomal RNA Isolation Kit from exosomes enriched using Norgen's Cell Culture Media Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for biomarker discovery. Exosomal RNA isolation is based on Norgen's proprietary resin without the need for phenol extractions or carrier RNA.

Exosomes isolated from cell culture media at Norgen

Kit Specifications
Cell Culture Media Input (Cat. 60400)	5 mL - 10 mL
Cell Culture Media Input (Cat. 60500)	10 mL - 20 mL
Cell Culture Media Input (Cat. 60600)	20 mL - 35 mL
Size of Exosomes Purified	40 nm - 150 nm
Elution Volume	50 - 100 µL
Time to Complete 10 Purifications	30 - 45 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.

Kit Components

Component	Cat. 60400 (50 preps)	Cat. 60500 (25 preps)	Cat. 60600 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	1.5 mL	1.5 mL	1.5 mL
ExoR Buffer	12 mL	12 mL	12 mL
Mini Filter Spin Columns	50	25	15
Elution Tubes (1.7 mL)	50	25	15
Product Insert	1	1	1