Cell Culture Media Exosome Purification Kits

Overview

Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
Versatile sample input ranging from 5 mL to 35 mL
- Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL)
- Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL)
Exosome purification is based on Norgen’s proprietary resin
No precipitation reagents, overnight incubation, protease or coagulant treatments required
No time-consuming ultracentrifugation, filtration or special syringes required
Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
Purified exosomes are compatible with functional studies.
Purified exosomes are free from any other RNA-binding proteins
Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or another RNA extraction method.

The Cell Culture Media Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for intact exosomes from different cell culture media sample volumes ranging from 5 mL to 35 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different cell culture media sample volumes, and these EVs are ready for most downstream applications. The purification is based on Norgen's proprietary resin.

These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal transcripts.

NanoSight® Analysis

Exosomes enriched with Norgen's Cell Culture Media Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration

Exosomal RNA Analysis

To purify exosomes and isolate exosomal RNA, choose the Cell culture media Exosome Purification and RNA isolation kits. The protocol is divided into 2 parts and an aliquot of purified exosomes can be taken for applications like NTA / TEM etc before processing them for RNA isolation. Or you can use the Exosomal RNA isolation kit if you’ve already purified exosomes using a Norgen kit or another method. Exosomal RNA isolation is based on Norgen’s proprietary resin without the need for phenol extractions or carrier RNA. This RNA is ideal for gene expression analysis using RT-qPCR, microarray, or NGS and for biomarker discovery.

Exosomes isolated from cell culture media at Norgen

Details

Supporting Data

Figure 1. Isolation of RNA from exosomes purified from different cell culture media volumes. Norgen's Cell Culture Media Exosome Purification Mini Kit (Cat. 60400) was used to isolate exosomal RNA from exosomes purified from different cell culture media volumes using the same kit. 2 µL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated exosomal RNA (A) The exosomal avg. Ct value for miR-26a is linearly decreasing with increasing the sample input volume. (B) The relative amount of the exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%

Figure 2. Isolation of RNA from exosomes purified from different cell culture media volumes. Norgen's Cell Culture Media Exosome Purification Maxi Kit (Cat. 60600) was used to isolate exosomal RNA from different cell culture media volumes from exosomes purified using the same kit. 2 µL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated exosomal RNA. (A) The avg. Ct value for exosomal miR-26a is linearly decreasing with increasing the sample input volume. (B) The relative amount of the exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%.

Purification and enrichment of intact cell culture media exosomes for functional studies
Versatile sample input ranging from 5 mL to 35 mL
- Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL)
- Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL)
- Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL)
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents or overnight incubation required
No protease treatment required
Compatible with cell culture media from any species
Pure exosomes are purified and are free-from any other RNA-binding proteins
Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

Storage Conditions

All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 60400 (50 preps)	Cat. 60600 (15 preps)
Slurry E	12.5 mL	12.5 mL
ExoC Buffer	1.5 mL	1.5 mL
ExoR Buffer	12 mL	12 mL
Mini Filter Spin Columns inserted into 2 mL tubes	50	15
Product Insert	1	1

Documentation

RELATED BLOGS

FAQs

Citations

Title	Adipose-Derived Mesenchymal Stem Cell-Derived Extracellular Vesicles Rescue Tendon Injury in Rat via the miR-19 a/IGFBP3 Axis
Journal	Stem Cells International. 2022.
Authors	Haibo Zhao, Hongyuan Jiang, Haoyun Zhang, Zewen Sun, Qian Lin, Tianrui Wang ,Tengbo Yu and Yingze Zhang

Title	Comparative Proteomic Profiling of Secreted Extracellular Vesicles from Breast Fibroadenoma and Malignant Lesions: A Pilot Study
Journal	Int. J. Mol. Sci.. 2022.
Authors	Katia Pane, Cristina Quintavalle, Silvia Nuzzo, Francesco Ingenito, Giuseppina Roscigno, Alessandra Affinito, Iolanda Scognamiglio, Birlipta Pattanayak, Enrico Gallo, Antonella Accardo, Guglielmo Thomas, Zoran Minic, Maxim V. Berezovski, Monica Franzese, and Gerolama Condorelli

Title	Vitamin D Reverts the Exosome-Mediated Transfer of Cancer Resistance to the mTOR Inhibitor Everolimus in Hepatocellular Carcinoma
Journal	Front Oncol. 2022.
Authors	Mariarosaria Negri, Feliciana Amatrudo, Annalisa Gentile, Roberta Patalano, Tatiana Montò, Cristina de Angelis, Chiara Simeoli, Rosa Pirchio, Renata Simona Auriemma, Annamaria Colao, Rosario Pivonello, and Claudia Pivonello

Title	Comparative Proteomic Profiling of Secreted Extracellular Vesicles from Breast Fibroadenoma and Malignant Lesions: A Pilot Study
Journal	Int. J. Mol. Sci. April 2022.
Authors	Pane K., Quintavalle C., Nuzzo S., Ingenito F., Roscigno G., Affinito A., Scognamiglio I., Pattanayak B., Gallo E., Accardo A. and Thomas G.,

Title	Small RNA sequencing and bioinformatics analysis of RAW264.7-derived exosomes after Mycobacterium Bovis Bacillus Calmette-Guérin infection
Journal	BMC Genomics. May 2022.
Authors	Zhan, Xuehua, Wenqi Yuan, Yueyong Zhou, Rong Ma, and Zhaohui Ge.

Title	Microarray analysis of circRNAs sequencing profile in exosomes derived from bone marrow mesenchymal stem cells in postmenopausal osteoporosis patients
Journal	JCLA. 2021.
Authors	Miao Fu,Liping Fang,Xi Xiang,Xijing Fan,Junqi Wu,Jinhua Wang

Title	Protective role of mesenchymal stem cells and mesenchymal stem cell-derived exosomes in cigarette smoke-induced mitochondrial dysfunction in mice
Journal	Toxicol Appl Pharmacol. 2019.
Authors	Krishna Prahlad Maremanda, Isaac K. Sundar, and Irfan Rahman*

Title	BK Polyomavirus MicroRNA Levels in Exosomes Are Modulated by Non-Coding Control Region Activity and Down-Regulate Viral Replication When Delivered to Non-Infected Cells Prior to Infection
Journal	Viruses. September 2018.
Authors	Francesco Martelli, Zongsong Wu, Serena Delbue, Fabian H. Weissbach, Maria Chiara Giulioli, Pasquale Ferrante, Hans H. Hirsch, and Simone Giannecchini

Cell Culture Media Exosome Purification Kits