Cell Culture Media Exosome Purification Kits

概述

The Cell Culture Media Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for intact exosomes from different cell culture media sample volumes ranging from 5 mL to 35 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different cell culture media sample volumes, and these EVs are ready for most downstream applications. The purification is based on Norgen's proprietary resin.

These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal transcripts.

NanoSight® Analysis

Exosomes enriched with Norgen's Cell Culture Media Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration

Exosomal RNA Analysis

To purify exosomes and isolate exosomal RNA, choose the Cell culture media Exosome Purification and RNA isolation kits. The protocol is divided into 2 parts and an aliquot of purified exosomes can be taken for applications like NTA / TEM etc before processing them for RNA isolation. Or you can use the Exosomal RNA isolation kit if you’ve already purified exosomes using a Norgen kit or another method. Exosomal RNA isolation is based on Norgen’s proprietary resin without the need for phenol extractions or carrier RNA. This RNA is ideal for gene expression analysis using RT-qPCR, microarray, or NGS and for biomarker discovery.

Exosomes isolated from cell culture media at Norgen

细节

Supporting Data

Figure 1. Isolation of RNA from exosomes purified from different cell culture media volumes. Norgen's Cell Culture Media Exosome Purification Mini Kit (Cat. 60400) was used to isolate exosomal RNA from exosomes purified from different cell culture media volumes using the same kit. 2 µL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated exosomal RNA (A) The exosomal avg. Ct value for miR-26a is linearly decreasing with increasing the sample input volume. (B) The relative amount of the exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%

Figure 2. Isolation of RNA from exosomes purified from different cell culture media volumes. Norgen's Cell Culture Media Exosome Purification Maxi Kit (Cat. 60600) was used to isolate exosomal RNA from different cell culture media volumes from exosomes purified using the same kit. 2 µL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated exosomal RNA. (A) The avg. Ct value for exosomal miR-26a is linearly decreasing with increasing the sample input volume. (B) The relative amount of the exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%.

Purification and enrichment of intact cell culture media exosomes for functional studies
Versatile sample input ranging from 5 mL to 35 mL
- Cell Culture Media Exosome Purification Mini Kit (5 mL - 10 mL)
- Cell Culture Media Exosome Purification Midi Kit (10 mL - 20 mL)
- Cell Culture Media Exosome Purification Maxi Kit (20 mL - 35 mL)
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents or overnight incubation required
No protease treatment required
Compatible with cell culture media from any species
Pure exosomes are purified and are free-from any other RNA-binding proteins
Purified exosomes can be analyzed using NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

Storage Conditions

All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 60400 (50 preps)	Cat. 60600 (15 preps)
Slurry E	12.5 mL	12.5 mL
ExoC Buffer	1.5 mL	1.5 mL
ExoR Buffer	12 mL	12 mL
Mini Filter Spin Columns inserted into 2 mL tubes	50	15
Product Insert	1	1

Cell Culture Media Exosome Purification Kits