Clostridium difficile TaqMan PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz Clostridium difficile TaqMan PCR Detection Kit Dx Price USD$325.00 SKU DxTM37100 Format PCR Size 24 Reactions Quantity Overview Details Documentation Citations Overview Detection kit for Clostridium difficile CE-IVDD marked in accordance with the European Commission Directive 98/79/EC. Research version available Available in TaqMan format for analysis Clostridium difficile is rod-shaped, gram positive bacterium. It is the main causal agent of antibiotic-associated diarrhea and pseudomembranous colitis. The colonization of intestines by C. difficile is usually associated with the elimination of natural intestinal flora as a result of antibiotic application and is frequently reported in health care centers. While C. difficile infection could be severe and life-threatening, particularly among the elderly, many patients are asymptomatic making diagnosis challenging during outbreaks. The tradition method of detecting C. difficile infection is by cytotoxicity test of the toxin produced by the bacterium, but such protocols usually require extensive time before conclusion can be made. TaqMan PCR Kit Click to expand options Clostridium difficile TaqMan PCR Kit, 24 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions The Clostridium difficile TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component TaqMan Probe Cat. DxTM37100 (24 rxns) MDx TaqMan 2X PCR Master Mix Dx 550 µL Clostridium difficile Primer & Probe Mix Dx 2 x 70 µL Clostridium difficile Positive Control Dx - 200,000 copies/μL 50 µL Nuclease-Free Water 2 x 1.25 mL Product Insert 1 Documentation PROTOCOLS (DxTM37100) Clostridium difficile TaqMan PCR Kit Dx - Protocol (24 rxns) SAFETY DATA SHEETS (DxTM37100) Clostridium difficile TaqMan PCR Kit Dx - Safety Data Sheet Citations Title Gut health of children with autism spectrum disorder Journal World Academy of Sciences Journal.2022. Authors Elene Kakabadze, Khatuna Makalatia, Nata Bakuradze, Nino Grdzelishvili, Sophie Kereselidze, Tamar Ediberidze, Veriko Berulava, Nana Sabauri, Nana Cholokashvili, Elene Javakhishvili, Gulnara Natroshvili, Tamar Tatrishvili, Mariam Motsikulashvili, Nina Chanishvili, Ivane Abiatari Related Products Clostridium difficile TaqMan Probe/Primer and Control Set Stool DNA Isolation Kit
Clostridium difficile TaqMan PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz Clostridium difficile TaqMan PCR Detection Kit Dx Price USD$325.00 SKU DxTM37100 Format PCR Size 24 Reactions Quantity
