Clostridium difficile TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Clostridium difficile TaqMan PCR Detection Kits Price USD$699.00 SKU TM37150 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for Clostridium difficile CE-IVD marked version available for in vitro diagnostic use Available in TaqMan format for analysis Clostridium difficile is rod-shaped, gram positive bacterium. It is the main causal agent of antibiotic-associated diarrhea and pseudomembranous colitis. The colonization of intestines by C. difficile is usually associated with the elimination of natural intestinal flora as a result of antibiotic application and is frequently reported in health care centers. While C. difficile infection could be severe and life-threatening, particularly among the elderly, many patients are asymptomatic making diagnosis challenging during outbreaks. The tradition method of detecting C. difficile infection is by cytotoxicity test of the toxin produced by the bacterium, but such protocols usually require extensive time before conclusion can be made. TaqMan PCR Kit Click to expand options Clostridium difficile TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Clostridium difficile TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM37150 (100 preps) Cat. TM37110 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - Clostridium difficile Primer & Probe Mix 280 μL 280 μL Clostridium difficile Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM37150) Clostridium difficile TaqMan PCR Kit - Protocol (100 rxns) (TM37110) Clostridium difficile TaqMan Probe/Primer and Control Set - Protocol (100 rxns) SAFETY DATA SHEETS (TM37150) Clostridium difficile TaqMan PCR Kit - Safety Data Sheet Related Products Clostridium difficile TaqMan PCR Kit Dx Clostridium difficile TaqMan Probe/Primer and Control Set Stool DNA Isolation Kit
Clostridium difficile TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Clostridium difficile TaqMan PCR Detection Kits Price USD$699.00 SKU TM37150 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity
