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Purchasing Information


  • Detection kits for the Clostridium difficile
  • Available in 2 formats for analysis:
 

ItemsCat. #Size
Clostridium difficile TaqMan PCR Kit TM37100 24 rxns
Clostridium difficile TaqMan Probe/Primer and Control Set TM37110 100 rxns
Clostridium difficile End-Point PCR Kit EP37100 24 rxns
Clostridium difficile Primer and Control Set EP37110 100 rxns

  • Detection kits for the Clostridium difficile
  • Available in 2 formats for analysis:
 


Clostridium difficile Detection Kits

Clostridium difficile is rod-shaped, gram positive bacterium. It is the main causal agent of antibiotic-associated diarrhea and pseudomembranous colitis. The colonization of intestines by C. difficile is usually associated with the elimination of natural intestinal flora as a result of antibiotic application and is frequently reported in health care centers. While C. difficile infection could be severe and life-threatening, particularly among the elderly, many patients are asymptomatic making diagnosis challenging during outbreaks. The tradition method of detecting C. difficile infection is by cytotoxicity test of the toxin produced by the bacterium, but such protocols usually require extensive time before conclusion can be made.

There are 3 kits available for the detection of Clostridium difficile:


Click to expand options
Clostridium difficile TaqMan PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents
Clostridium difficile TaqMan PCR/RT-PCR Probe/Primer Set and Controls, 100 reactions
  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

Click to expand options
Clostridium difficile End-Point PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer mix for the pathogen/virus/viroid of interest
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate qualitative analysis of the results on an agarose gel
Clostridium difficile End-Point PCR/RT-PCR Primer Set and Controls, 100 reactions
  • Specific Primer mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.


ItemsCat. #Size
Clostridium difficile TaqMan PCR Kit TM37100 24 rxns
Clostridium difficile TaqMan Probe/Primer and Control Set TM37110 100 rxns
Clostridium difficile End-Point PCR Kit EP37100 24 rxns
Clostridium difficile Primer and Control Set EP37110 100 rxns

  • Detection kits for the Clostridium difficile
  • Available in 2 formats for analysis:
 


Clostridium difficile Detection Kits

Clostridium difficile is rod-shaped, gram positive bacterium. It is the main causal agent of antibiotic-associated diarrhea and pseudomembranous colitis. The colonization of intestines by C. difficile is usually associated with the elimination of natural intestinal flora as a result of antibiotic application and is frequently reported in health care centers. While C. difficile infection could be severe and life-threatening, particularly among the elderly, many patients are asymptomatic making diagnosis challenging during outbreaks. The tradition method of detecting C. difficile infection is by cytotoxicity test of the toxin produced by the bacterium, but such protocols usually require extensive time before conclusion can be made.

There are 3 kits available for the detection of Clostridium difficile:


Click to expand options
Clostridium difficile TaqMan PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents
Clostridium difficile TaqMan PCR/RT-PCR Probe/Primer Set and Controls, 100 reactions
  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

Click to expand options
Clostridium difficile End-Point PCR/RT-PCR Kit, 24 reactions
  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer mix for the pathogen/virus/viroid of interest
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate qualitative analysis of the results on an agarose gel
Clostridium difficile End-Point PCR/RT-PCR Primer Set and Controls, 100 reactions
  • Specific Primer mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR/RT-PCR Master Mix (#28007/#28113)or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.

 

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