COVID-19/Influenza (A & B) TaqMan RT-PCR Detection Kit For research use only and NOT intended for in vitro diagnostics.Detect SARS-CoV-2 and Influenza A + B in one convenient kit Powered by Bioz COVID-19/Influenza (A & B) TaqMan RT-PCR Detection Kit Price USD$2,899.00 SKU TM67400 Format RT-PCR Size 500 Reactions Quantity Overview Details Documentation Citations Overview Multiplex RT-PCR system detects SARS-CoV-2 specific gene (N gene), Influenza A (M gene) and Influenza B (HA gene) in a single one step RT-PCR reaction Primer & Probe Mixes contain all N gene, M gene, HA gene and human RP assays Positive Control contains the SARS-related target genes: N gene, Influenza A target gene (M gene), Influenza B target gene (HA gene) as well as the human RP (internal control) Convenient ready-to-use 2X One-Step RT-PCR Master Mix included in kit Manufactured under ISO 13485:2016 for research use only CE-IVD marked version pending View High Resolution Data Norgen’s COVID-19/Influenza (A & B) TaqMan RT-PCR Kit includes 2X One-Step RT-PCR Master Mix, a primer/probe mix, a positive control and a negative control (nuclease-free water). The primer/probe mix contains SARS-CoV-2 detection target; N (Nucleoprotein), Influenza A detection target; M gene and Influenza B detection target; HA (Hemaglutinin) and the human RNase P transcript as an internal control target to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The provided N/Inf A & B/RP Positive Control contains an in vitro RNA transcript for the SARS-related target gene (N Gene), and genes associated with Influenza A (M gene) and Influenza B (HA Gene), as well as the human RP gene (internal control). Norgen’s COVID-19/Influenza (A & B) TaqMan RT-PCR Kit was developed and validated to be used with the following PCR instruments: Qiagen Rotor-Gene Q, BioRad CFX96 Touch™ Real-Time PCR Detection System, ABI 7500, ABI Quantstudio 5 and 7. Details Supporting Data Previous Limit of Detection of Norgen’s COVID-19/Influenza (A & B) TaqMan RT-PCR Kit. PCR amplification (Ct values) of purified nasopharyngeal swabs (A), oropharyngeal swabs (B) and saliva samples (C) were spiked with SARS-CoV-2, Influenza A and Influenza B viral particles at 8X, 80X, 800X and 8,000X LoD. Norgen’s COVID-19/Influenza Kit can detect viruses from nasopharyngeal, oropharyngeal or saliva samples with similar Ct values. Robustness of Norgen’s COVID-19/Influenza (A & B) TaqMan RT-PCR Kit. A) PCR amplification (Ct values) of purified nasopharyngeal swabs were spiked with SARS-CoV-2, Influenza A and Influenza B viral particles at 3X LoD. B) PCR amplification (Ct values) of purified saliva samples were spiked with viral particles at 3X LoD. Various treatments with or the addition of interfering substances into nasopharyngeal (A) or saliva (B) samples did not affect the Ct values. In addition, Norgen’s COVID-19/Influenza Kit can detect viruses from nasopharyngeal or saliva samples with similar Ct values. Freeze/Thaw Stabilty of Norgen’s COVID-19/Influenza (A & B) TaqMan RT-PCR Kit. Norgen’s COVID-19/Influenza Kit is stable for virus detection with at least 4 freeze/thaw cycles. SARS-CoV-2, Influenza A and Influenza B viral particles at 2X LoD and 5X LoD were spiked into nasopharyngeal swabs. The purified samples were detected using Norgen’s COVID-19/Influenza (A & B) TaqMan RT-PCR Kit which were subjected to 1, 2, 3 and 4 freeze/thaw cycles. Across 4 cycles of freeze/thaw, there was no apparent change in Ct values. Next Click for expanded view Storage Conditions and Product Stability All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance. Kit Component Cat. TM67400 (500 preps) N/Inf A & B/RP Primer & Probe Mix 850 μL N/Inf A & B/RP Positive Control † 500 μL 2X One-Step RT-PCR Master Mix 9 x 1 mL Nuclease-Free Water (Negative Control) 2 x 1.25 mL Product Insert 1 † Contains an in vitro RNA transcript for the SARS-CoV-2 N gene, Influenza M and HA genes and RNase P RNA fragments. Documentation PROTOCOLS (TM67400) COVID-19/Influenza (A & B) TaqMan RT-PCR Kit - Protocol (500 rxns) Related Products Total Nucleic Acid Preservation Tubes Saliva RNA Collection and Preservation Devices Total RNA Purification Kit
COVID-19/Influenza (A & B) TaqMan RT-PCR Detection Kit For research use only and NOT intended for in vitro diagnostics.Detect SARS-CoV-2 and Influenza A + B in one convenient kit Powered by Bioz COVID-19/Influenza (A & B) TaqMan RT-PCR Detection Kit Price USD$2,899.00 SKU TM67400 Format RT-PCR Size 500 Reactions Quantity
