Fatty Tissue RNA Purification Kit For purification of total RNA (including microRNA) from animal tissues with high lipid content For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Fatty Tissue RNA Purification Kit For purification of total RNA (including microRNA) from animal tissues with high lipid content Price USD$229.00 SKU 36200 Format Spin Column Size 25 Preps Quantity Request sample Overview Details Documentation Citations Overview Isolate high quality total RNA from hard to extract fatty tissues Isolate total RNA, including microRNA (miRNA) No phenol or chloroform extractions Fast and easy processing using rapid spin-column format Genomic DNA removal without the use of nucleases Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix This kit was deigned to extract high quality and yield from fatty tissues like brain, breast, adipose tissues, etc. Often tissues that have high fat contents like brain, breast tissues, etc., can lead to low quality and yield RNA with no-optimized kit.This kit provides a rapid and optimized method for the isolation and purification of total RNA from animal tissues with high lipid content, including brain and adipose tissues. The kit purifies all sizes of RNA down to microRNA (miRNA) without the use of inhibitory phenol or chloroform. While the kit is optimized for fatty tissues, it can also be used for most mammalian tissue samples.The purified RNA is of the highest integrity, and can be used in a number of downstream applications including RT-qPCR, microarrays, NGS. Details Supporting Data Previous Figure 1. Isolation of High Quality Brain Total RNA including microRNA without the use of Phenol. Norgen's Fatty Tissue RNA Purification Kit isolates high quality total RNA from fatty tissues such as brain without the use of phenol. Total RNA was isolated from equal amounts of brain tissue using Norgen’s Fatty Tissue RNA Purification Kit and a leading competitor’s kit that involves a phenol:chloroform extraction step. The purified RNA was then resolved on a 1.2% formaldehyde-agarose gel. As it can be seen, Norgen not only isolated high and consistent yields of total RNA, but the RNA was also of high quality as evidenced by intactness of the major 28S and 18S rRNA. Importantly, only Norgen's Fatty Tissue RNA Purification Kit was able to recover the small RNA fraction, including the microRNA (red-boxed). Figure 2. High Yield of a Diversity of RNA Species. Norgen's Fatty Tissue RNA Purification Kit effectively recovers all sizes of RNA from large mRNA to small RNA, including microRNAs. Total RNA was isolated from equal amounts of brain tissue using Norgen's Fatty Tissue RNA Purification Kit and a leading competitor’s kit that involves a phenol:chloroform extraction step. The purified RNA was then used as the template in a RT-qPCR for detecting the beta-Actin gene (Top Panel) and for detecting miR-21 (Bottom Panel). In both graphs the blue lines correspond to Norgen isolated-RNA and the red lines correspond to competitor-isolated RNA. As it can be seen, Norgen's kit isolated higher yields of microRNA, as indicated by the lower Ct values of the blue lines (Top Panel). Also, Norgen's kit successfully isolated similar amount of large RNA to that of the competitor's kit (Bottom Panel) indicating the full diversity of RNA species isolated. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity 50 µg Maximum Column Loading Volume 650 µL Size of RNA Purified All sizes, including small RNA (<200 nt) Maximum Amount of Starting Material: Brain and Adipose Tissues 20 mg Time to Complete 10 Purifications 50 minutes Average Yield* Brain (15 mg) 15 µg *Average Yield will vary depending on a number of factors including species, growth conditions used, and developmental stage. Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 36200 (25 preps) Buffer RL 30 mL Lysis Additive B 1 mL RNase-Free Water 15 mL Wash Solution A 38 mL Elution Solution A 6 mL Mini Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (36200) Fatty Tissue RNA Purification Kit - Protocol (25 preps) APPLICATION NOTES Fatty Tissue RNA Purification Kit Revised Guidelines For RNA Quality Assessment For Diverse Biological Sample Input - Application Note Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input SAFETY DATA SHEETS (36200) Fatty Tissue RNA Purification Kit - Safety Data Sheet POSTERS Fatty Tissue RNA Purification Kit Revised Guidelines For RNA Quality Assessment For Diverse Biological Sample Input - Poster FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Title Retinoic Acid Receptor Responder Protein 2 and Intelectin‑1 in Visceral Adipose Tissue from Pregnant Women with Gestational Diabetes Mellitus Journal Med J DY Patil Vidyapeeth. 2022 Authors Betsy Corina Sosa García, Araceli Consuelo Hinojosa Juárez, María del Carmen García García, Carlos Jhovani Pérez‑Amado, Silvia Jiménez‑Morales, Hugo Mendieta Zerón Title Subcutaneous adipose tissue splice quantitative trait loci reveal differences in isoform usage associated with cardiometabolic traits Journal The American Journal of Human Genetics. 2022 Authors Sarah M. Brotman, Chelsea K. Raulerson, Swarooparani Vadlamudi, Kevin W. Currin, Qiujin Shen, Victoria A. Parsons, Apoorva K. Iyengar, Tamara S. Roman, Terrence S. Furey, Johanna Kuusisto, Francis S. Collins, Michael Boehnke, Markku Laakso, Päivi Pajukanta, Karen L.Mohlke Title Seasonal heat stress affects adipose tissue proteome toward enrichment of the Nrf2-mediated oxidative stress response in late-pregnant dairy cows Journal Journal of Proteomics. 2017 Authors Zachut M, Kra G, Livshitz L, Portnick Y, Yakoby S, Friedlander G, Levin Y. Title MicroRNA expression is altered in lateral septum across reproductive stages Journal Neuroscience. 2015. Authors Saul MC, Zhao C, Driessen TM, Eisinger BE, Gammie SC Title Hypothalamic neuropeptide Y (NPY) gene expression is not affected by central serotonin in the rainbow trout (Oncorhynchus mykiss). Journal Comparative Biochemistry and Physiology. 2013. Authors Mancebo MJ, Ceballos FC, Perez-Maceira J, Aldegunde M. 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Fatty Tissue RNA Purification Kit For purification of total RNA (including microRNA) from animal tissues with high lipid content For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Fatty Tissue RNA Purification Kit For purification of total RNA (including microRNA) from animal tissues with high lipid content Price USD$229.00 SKU 36200 Format Spin Column Size 25 Preps Quantity Request sample
