Fatty Tissue RNA Purification Kit (Cat. 36200)
Fatty Tissue RNA Purification Kit
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Fatty Tissue RNA Purification Kit
For purification of total RNA (including microRNA) from animal tissues with high lipid content
- Isolate high quality total RNA from hard to extract fatty tissues
- Isolate total RNA, including microRNA (miRNA)
- No phenol or chloroform extractions
- Fast and easy processing using rapid spin-column format
- Genomic DNA removal without the use of nucleases
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 36200) 25 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
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Kit Specifications
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity
|
50 µg
|
|
Maximum Column Loading Volume
|
650 µL
|
|
Size of RNA Purified
|
All sizes, including small RNA
(<200 nt) |
| Maximum Amount of Starting Material: Brain and Adipose Tissues |
20 mg |
| Time to Complete 10 Purifications |
50 minutes
|
|
Average Yield*
Brain (15 mg) |
15 µg |
*Average Yield will vary depending on a number of factors including species, growth conditions used, and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Supporting Data
| Title | Retinoic Acid Receptor Responder Protein 2 and Intelectin‑1 in Visceral Adipose Tissue from Pregnant Women with Gestational Diabetes Mellitus |
| Journal | Med J DY Patil Vidyapeeth. 2022 |
| Authors | Betsy Corina Sosa García, Araceli Consuelo Hinojosa Juárez, María del Carmen García García, Carlos Jhovani Pérez‑Amado, Silvia Jiménez‑Morales, Hugo Mendieta Zerón |
| Title | Subcutaneous adipose tissue splice quantitative trait loci reveal differences in isoform usage associated with cardiometabolic traits |
| Journal | The American Journal of Human Genetics. 2022 |
| Authors | Sarah M. Brotman, Chelsea K. Raulerson, Swarooparani Vadlamudi, Kevin W. Currin, Qiujin Shen, Victoria A. Parsons, Apoorva K. Iyengar, Tamara S. Roman, Terrence S. Furey, Johanna Kuusisto, Francis S. Collins, Michael Boehnke, Markku Laakso, Päivi Pajukanta, Karen L.Mohlke |
| Title | Seasonal heat stress affects adipose tissue proteome toward enrichment of the Nrf2-mediated oxidative stress response in late-pregnant dairy cows |
| Journal | Journal of Proteomics. 2017 |
| Authors | Zachut M, Kra G, Livshitz L, Portnick Y, Yakoby S, Friedlander G, Levin Y. |
| Title | MicroRNA expression is altered in lateral septum across reproductive stages |
| Journal | Neuroscience. 2015. |
| Authors | Saul MC, Zhao C, Driessen TM, Eisinger BE, Gammie SC |
| Title | Hypothalamic neuropeptide Y (NPY) gene expression is not affected by central serotonin in the rainbow trout (Oncorhynchus mykiss). |
| Journal | Comparative Biochemistry and Physiology. 2013. |
| Authors | Mancebo MJ, Ceballos FC, Perez-Maceira J, Aldegunde M. |
Fatty Tissue RNA Purification Kit
This kit was deigned to extract high quality and yield from fatty tissues like brain, breast, adipose tissues, etc. Often tissues that have high fat contents like brain, breast tissues, etc., can lead to low quality and yield RNA with no-optimized kit.
This kit provides a rapid and optimized method for the isolation and purification of total RNA from animal tissues with high lipid content, including brain and adipose tissues. The kit purifies all sizes of RNA down to microRNA (miRNA) without the use of inhibitory phenol or chloroform. While the kit is optimized for fatty tissues, it can also be used for most mammalian tissue samples.
The purified RNA is of the highest integrity, and can be used in a number of downstream applications including RT-qPCR, microarrays, NGS.
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Column Binding Capacity
|
50 µg
|
|
Maximum Column Loading Volume
|
650 µL
|
|
Size of RNA Purified
|
All sizes, including small RNA
(<200 nt) |
| Maximum Amount of Starting Material: Brain and Adipose Tissues |
20 mg |
| Time to Complete 10 Purifications |
50 minutes
|
|
Average Yield*
Brain (15 mg) |
15 µg |
*Average Yield will vary depending on a number of factors including species, growth conditions used, and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Title | Retinoic Acid Receptor Responder Protein 2 and Intelectin‑1 in Visceral Adipose Tissue from Pregnant Women with Gestational Diabetes Mellitus |
| Journal | Med J DY Patil Vidyapeeth. 2022 |
| Authors | Betsy Corina Sosa García, Araceli Consuelo Hinojosa Juárez, María del Carmen García García, Carlos Jhovani Pérez‑Amado, Silvia Jiménez‑Morales, Hugo Mendieta Zerón |
| Title | Subcutaneous adipose tissue splice quantitative trait loci reveal differences in isoform usage associated with cardiometabolic traits |
| Journal | The American Journal of Human Genetics. 2022 |
| Authors | Sarah M. Brotman, Chelsea K. Raulerson, Swarooparani Vadlamudi, Kevin W. Currin, Qiujin Shen, Victoria A. Parsons, Apoorva K. Iyengar, Tamara S. Roman, Terrence S. Furey, Johanna Kuusisto, Francis S. Collins, Michael Boehnke, Markku Laakso, Päivi Pajukanta, Karen L.Mohlke |
| Title | Seasonal heat stress affects adipose tissue proteome toward enrichment of the Nrf2-mediated oxidative stress response in late-pregnant dairy cows |
| Journal | Journal of Proteomics. 2017 |
| Authors | Zachut M, Kra G, Livshitz L, Portnick Y, Yakoby S, Friedlander G, Levin Y. |
| Title | MicroRNA expression is altered in lateral septum across reproductive stages |
| Journal | Neuroscience. 2015. |
| Authors | Saul MC, Zhao C, Driessen TM, Eisinger BE, Gammie SC |
| Title | Hypothalamic neuropeptide Y (NPY) gene expression is not affected by central serotonin in the rainbow trout (Oncorhynchus mykiss). |
| Journal | Comparative Biochemistry and Physiology. 2013. |
| Authors | Mancebo MJ, Ceballos FC, Perez-Maceira J, Aldegunde M. |
