microRNA Purification Kit
For the rapid purification of microRNA without phenol
For research use only and NOT intended for in vitro diagnostics.
microRNA Purification Kit
For the rapid purification of microRNA without phenol
Overview
- Isolate and extract high quality & quantity miRNA in 30 minutes
- Two column kit- eliminate large RNA on the first column and capture microRNA on a second column
- Elute microRNA in 25 µL ready for miRNA profiling
- Process a wide range of samples- cell, tissue, bacteria, bodily fluids, etc.
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
This kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured animal cells, small tissue samples, bacterial cells, plants, and blood.
Two columns are provided with this kit. The first column captures large RNA, while the small RNA are captured on a second column and are eluted concentrated in 25 µL of nuclease-free water.
The small RNA can be used in various downstream applications relating to miRNA profiling, gene regulation, and functional analysis. The eluted RNA is ready for RT-qPCR, microarrays, and NGS applications.
Background
These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long and regulate gene expression by binding to mRNA molecules and affecting their stability or translation.
Details
Supporting Data
Kit Specifications
|
|
Maximum Column Binding Capacity
|
Up to 50 μg RNA
|
Maximum Column Loading Volume
|
650 μL
|
Minimum Elution Volume | 20 μL |
Size of RNA Purified |
All sizes, including small RNA < 200 nt
|
Time to Complete 10 Purifications |
25 minutes
|
Maximum Amount of Starting Material: Animal Cells Animal Tissues Bacteria Plant Tissues Blood |
3 x 106 cells 5-25 mg 1 x 109 cells 50 mg 100 μL |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 21300 (25 preps) |
---|---|
Buffer RL | 40 mL |
Wash Solution A | 38 mL |
Elution Solution A | 6 mL |
Large RNA Removal Column | 25 |
microRNA Enrichment Column | 25 |
Collection Tube | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
Documentation
Citations
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Journal | Antioxidants. 2024. |
Authors | Serge Cedrick Toya Mbiandjeu, Angela Siciliano, Alessandro Mattè, Enrica Federti, Massimiliano Perduca, Davide Melisi, Immacolata Andolfo, Angela Amoresano, Achille Iolascon, Maria Teresa Valenti, Francesco Turrini, Michele Bovi, Arianna Pisani, Antonio Recchiuti, Domenico Mattoscio, Veronica Riccardi,Luca Dalle Carbonare, Carlo Brugnara, Narla Mohandas, and Lucia De Franceschi |
Title | Early Salivary miRNA Expression in Extreme Low Gestational Age Newborns |
Journal | Life. 2022. |
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Journal | Bioengineered. 2022. |
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Title | Cancer cells exploit an orphan RNA to drive metastatic progression |
Journal | Nature Medicine. 2018. |
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Title | Expression of miRNA-140 in Chondrocytes and Synovial Fluid of Knee Joints in Patients with Osteoarthritis |
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Journal | PLOS One. 2016. |
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Title | Methods of Detecting Lung Cancer |
Journal | United States Patent Application 20160108478. 2016. |
Authors | Delfour, Olivier, Michot, Bernard |
Title | The human PMR1 endonuclease stimulates cell motility by down regulating miR-200 family microRNAs |
Journal | Nucleic Acid Research. 2016. |
Authors | Gu SQ, Gallego-Perez D, McClory SP, Shi J, Han J, Lee LJ, Schoenberg DR |
Title | Analysis of hepatic microRNA alterations in response to hepatitis B virus infection and pegylated interferon alpha-2a treatment |
Journal | Experimental Biology and Medicine. 2016. |
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Title | MEMO1 drives cranial endochondral ossification and palatogenesis |
Journal | Developmental Biology. 2015. |
Authors | Van Otterloo E, Feng W, Jones KL, Hynes NE, Clouthier DE, Niswander L, Williams T |
Title | Human microRNAs profiling in response to influenza A viruses (subtypes pH1N1, H3N2, and H5N1) |
Journal | Experimental Biology and Medicine. 2015. |
Authors | Makkoch J, Poomipak W, Saengchoowong S, Khongnomnan K, Praianantathavorn K, Jinato T, Poovorawan Y, Payungporn S |
Title | Circulating microRNA Profiling Needs Further Refinement Before Clinical Use in Patients With Aortic Stenosis |
Journal | Valvular Heart Disease. 2015. |
Authors | Sean Coffey, MBBS, Michael J. A. Williams, MD, L. Vicky Phillips, Gregory T. Jones, PhD |
Title | Identification of MicroRNAs and Transcript Targets in Camelina sativa by Deep Sequencing and Computational Methods |
Journal | PLoS ONE. 2015. |
Authors | Saroj Poudel, Niranjan Aryal, Chaofu Lu |
Title | MicroRNA-181b regulates ALX/FPR2 expression and proresolution signaling in human macrophages |
Journal | The Journal of Biological Chemistry. 2014. |
Authors | Anna Maria Pierdomenico, Antonio Recchiuti, Felice Simiele, Marilina Codagnone, Veronica Cecilia Mari, Giovanni Davi and Mario Romano |
Title | Role of TLR13 in innate immune recognition of group B streptococci |
Journal | Infection and Immunity. 2014. |
Authors | Giacomo Signorino, Nastaran Mohammadi, Francesco Patanè, Marco Buscetta, Mario Venza, Isabella Venza, Giuseppe Mancuso, Angelina Midiri, Lena Alexopoulou, Giuseppe Teti, Carmelo Biondo and Concetta Beninati. |
Title | Quantification of Circulating miRNAs in Plasma: Effect of Preanalytical and Analytical Parameters on Their Isolation and Stability. |
Journal | The Journal of Molecular Diagnostics. 2013. |
Authors | Sourvinou IS, Markou A, Lianidou ES. |
Title | A Panel of four MicroRNAs accurately differentiates Malignant from Benign Indeterminate Thyroid Lesions on Fine Needle Aspiration. |
Journal | Clinical Cancer Research. 2012. |
Authors | Xavier M. Keutgen, Filippo Filicori, Michael J. Crowley, et al. |
Title | Effective inhibition of replication of infectious bursal disease virus by miRNAs delivered by vectors and targeting the VP2 gene |
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Journal | American Journal of BioMedicine. 2014. |
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Title | Expanding the annotation of zebrafish microRNAs based on small RNA sequencing |
Journal | Gene. 2014. |
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Title | AcvR1-mediated BMP signaling in second heart field is required for arterial pole development: Implications for myocardial differentiation and regional identity. |
Journal | Developmental Biology. 2014. |
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Title | Effective inhibition of infectious bursal disease virus replication by 1recombinant avian adeno-associated virus-delivered microRNAs. |
Journal | Journal of Genetic Virology. 90: 1417-1422. |
Authors | Wang Y, Sun H, Shen P, Zhang X, Xiaoli X. |