Rapid purification of microRNA without phenol; isolation and extraction of microRNA

  • Extract high quality & purity miRNA in 30 minutes
  • Two column kit- eliminate large RNA on first column and capture microRNA on a second column
  • Elute microRNA in 25 µL ready for miRNA profiling
  • Process a wide range of samples- cell, tissue, bacteria, bodily fluids, etc

ItemsCat. #Size
microRNA Purification Kit 21300 25 preps

microRNA Purification Kit

This kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured animal cells, small tissue samples, bacterial cells, plants and blood.

Two columns are provided with this kit. The first column captures large RNA, while the small RNA are captured on a second column and are eluted concentrated in 25 µL of nuclease-free water.

The small RNA can be used in various downstream applications relating to miRNA profiling, gene regulation and functional analysis. The eluted RNA is ready for RT-qPCR, microarrays, and NGS applications.

Background

These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation.

Kit Specifications
Maximum Column Binding Capacity
Up to 50 μg RNA
Maximum Column Loading Volume
650 μL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 10 Purifications
25 minutes
Maximum Amount of Starting Material:
Animal Cells
Animal Tissues
Bacteria
Plant Tissues
Blood
3 x 106 cells
5-25 mg
1 x 109 cells
50 mg
100 μL

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 21300 (25 preps)
Buffer RL 40 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Large RNA Removal Column 25
microRNA Enrichment Column 25
Collection Tube 25
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Expression of miRNA-140 in Chondrocytes and Synovial Fluid of Knee Joints in Patients with Osteoarthritis
Journal Chinese Medical Sciences Journal. 2016.
Authors Hai-Bo S, Yi Z, Zong-Ke Z, Fu-Xing P, Yan-Rong L, Jing-Qiu C, Bin S.
Title Genomewide Association Study of African Children Identifies Association of SCHIP1 and PDE8A with Facial Size and Shape
Journal PLOS One. 2016.
Authors Cole JB, Manyama M, Kimwaga E, Mathayo J, Larson JR, Liberton DK, Lukowiak K, Ferrara TM, Riccardi SL, Li M, Mio W, Prochazkova M,, Williams T, Li H, Jones KL, Klein OD, Santorico SA, Hallgrimsson B, Spritz RA
Title Methods of Detecting Lung Cancer
Journal United States Patent Application 20160108478. 2016.
Authors Delfour, Olivier, Michot, Bernard
Title The human PMR1 endonuclease stimulates cell motility by down regulating miR-200 family microRNAs
Journal Nucleic Acid Research. 2016.
Authors Gu SQ, Gallego-Perez D, McClory SP, Shi J, Han J, Lee LJ, Schoenberg DR
Title Analysis of hepatic microRNA alterations in response to hepatitis B virus infection and pegylated interferon alpha-2a treatment
Journal Experimental Biology and Medicine. 2016.
Authors Jinato T, Chuaypen N, Poomipak W, Praianantathavorn K, Makkoch J, Kiatbumrung R, Jampoka K, Tangkijvanich P, Payungporn S
Title MEMO1 drives cranial endochondral ossification and palatogenesis
Journal Developmental Biology. 2015.
Authors Van Otterloo E, Feng W, Jones KL, Hynes NE, Clouthier DE, Niswander L, Williams T
Title Human microRNAs profiling in response to influenza A viruses (subtypes pH1N1, H3N2, and H5N1)
Journal Experimental Biology and Medicine. 2015.
Authors Makkoch J, Poomipak W, Saengchoowong S, Khongnomnan K, Praianantathavorn K, Jinato T, Poovorawan Y, Payungporn S
Title Circulating microRNA Profiling Needs Further Refinement Before Clinical Use in Patients With Aortic Stenosis
Journal Valvular Heart Disease. 2015.
Authors Sean Coffey, MBBS, Michael J. A. Williams, MD, L. Vicky Phillips, Gregory T. Jones, PhD
Title Identification of MicroRNAs and Transcript Targets in Camelina sativa by Deep Sequencing and Computational Methods
Journal PLoS ONE. 2015.
Authors Saroj Poudel, Niranjan Aryal, Chaofu Lu
Title MicroRNA-181b regulates ALX/FPR2 expression and proresolution signaling in human macrophages
Journal The Journal of Biological Chemistry. 2014.
Authors Anna Maria Pierdomenico, Antonio Recchiuti, Felice Simiele, Marilina Codagnone, Veronica Cecilia Mari, Giovanni Davi and Mario Romano
Title Role of TLR13 in innate immune recognition of group B streptococci
Journal Infection and Immunity. 2014.
Authors Giacomo Signorino, Nastaran Mohammadi, Francesco Patanè, Marco Buscetta, Mario Venza, Isabella Venza, Giuseppe Mancuso, Angelina Midiri, Lena Alexopoulou, Giuseppe Teti, Carmelo Biondo and Concetta Beninati.
Title Quantification of Circulating miRNAs in Plasma: Effect of Preanalytical and Analytical Parameters on Their Isolation and Stability.
Journal The Journal of Molecular Diagnostics. 2013.
Authors Sourvinou IS, Markou A, Lianidou ES.
Title A Panel of four MicroRNAs accurately differentiates Malignant from Benign Indeterminate Thyroid Lesions on Fine Needle Aspiration.
Journal Clinical Cancer Research. 2012.
Authors Xavier M. Keutgen, Filippo Filicori, Michael J. Crowley, et al.
Title Effective inhibition of replication of infectious bursal disease virus by miRNAs delivered by vectors and targeting the VP2 gene
Journal Journal of Virological Methods.65(2):127-32. 2010.
Authors Wang Y, Sun H, Shen P, Zhang X, Xia X, Xia B.
Title Bacterial recognition by TLR7 in the lysosomes of conventional dendritic cells
Journal Nature Immunology.10,587 - 594. 2009.
Authors Mancuso G,Gambuzza M,Midiri A,Biondo C,Papasergi S,Akira S,Teti G, Beninati C.
Title Novel expression of microRNAs in serum samples of Iraqi breast cancer women
Journal American Journal of BioMedicine. 2014.
Authors Zaynab Saad; Muhammed Arif; Nahi Yassen; Hameed Jasim; Majed Jelawe; James Brown
Title Expanding the annotation of zebrafish microRNAs based on small RNA sequencing
Journal Gene. 2014.
Authors Thomas Desvignes, Michael J. Beam, Peter Batzel, Jason Sydes, John H. Postlethwait
Title AcvR1-mediated BMP signaling in second heart field is required for arterial pole development: Implications for myocardial differentiation and regional identity.
Journal Developmental Biology. 2014.
Authors Thomas PS, Rajderkar S, Lane J, Mishina Y, Kaartinen V.
Title Effective inhibition of infectious bursal disease virus replication by 1recombinant avian adeno-associated virus-delivered microRNAs.
Journal Journal of Genetic Virology. 90: 1417-1422.
Authors Wang Y, Sun H, Shen P, Zhang X, Xiaoli X.