Mycobacterium tuberculosis TaqMan PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz Mycobacterium tuberculosis TaqMan PCR Detection Kit Dx SKU DxTM42100 Format PCR Size 24 Reactions Contact Distributor Overview Details Documentation Citations Overview Detection kit for Mycobacterium tuberculosis CE-IVDD marked in accordance with the European Commission Directive 98/79/EC. Research version available Available in TaqMan format for analysis Mycobacterium tuberculosis is a pathogenic bacterial species belonging to the genus Mycobacterium, and is the causative agent of tuberculosis. Tuberculosis (TB) is a multifaceted disease and challenging public health problem in both industrialized and developing countries. According to the WHO, 8.8 million active cases of TB are diagnosed each year and of these, almost 2 million die. Once thought to be under control or even close to extinction, TB infection levels are rising and the threat is compounded by new, virulent and drug-resistant strains. Although most cases occur in the developing world (22 countries accounting for 80% of all global cases), increasing population mobility combined with facility of transmission means that no country is immune from the resurgence of TB. TB control programs are currently facing a number of constraints. Worldwide, fewer than 25% of all tuberculosis cases are detected. Of utmost concern is the absence of a timely and accurate test for the diagnosis of mycobacterial disease. Early diagnosis is crucial for the prevention of further spread of the disease. TaqMan PCR Kit Click to expand options Mycobacterium tuberculosis TaqMan PCR Kit, 24 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Figure 4. Analytical Sensitivity. The analytical sensitivity of the Positive Control is evaluated through the amplification of a dilution series of the Positive Control Dx (provided, 200,000 copies/μL). Figure 5. Quantitative Analysis. The Ct values obtained from the amplification curves of the Positive Control were used to generate a standard curve that illustrates the linear relationship between the log Positive Control copy number (Log Starting Quantity, X axis) and the Ct value for each concentration (Cq, Y axis). Figure 6. Qualitative Analysis. Amplification curve of TaqMan PCR positive results. Effective results are demonstrated by the amplification of both Positive Control (circle) and Internal Control (square) above the baseline for both FAM (red) and HEX (purple) channels. Next Click for expanded view Storage Conditions The Mycobacterium tuberculosis TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. DxTM42100 (24 rxns) MDx TaqMan 2X PCR Master Mix Dx 550 µL M. tuberculosis Primer & Probe Mix Dx 2 x 70 µL M. tuberculosis Positive Control Dx - 200,000 copies/μL 50 µL Nuclease-Free Water 2 x 1.25 mL Product Insert 1 Documentation PROTOCOLS (DxTM42100) Mycobacterium tuberculosis TaqMan PCR Kit Dx - Protocol (24 rxns) SAFETY DATA SHEETS (DxTM42100) Mycobacterium tuberculosis TaqMan PCR Kit Dx - Safety Data Sheet Related Products Mycobacterium tuberculosis TaqMan Probe/Primer and Control Set Urine DNA Isolation Kit (Slurry Format)
Mycobacterium tuberculosis TaqMan PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz Mycobacterium tuberculosis TaqMan PCR Detection Kit Dx SKU DxTM42100 Format PCR Size 24 Reactions Contact Distributor
