For the rapid purification of total DNA from bacteriophages

  • Isolate high quality DNA from a broad variety of phage strains
  • High yields of total DNA
  • Fast and easy processing using a rapid spin-column format
  • No phenol or chloroform extractions or cesium chloride banding required
  • High yields of DNA recovered 10-30 µg DNA from 106-1010 pfu/ mL of enriched phages

ItemsCat. #Size
Phage DNA Isolation Kit 46800 50 preps
Phage DNA Isolation Kit 46850 100 Preps

Phage DNA Isolation Kit

This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. The spin-column based procedure is rapid and can be completed in less than 45 minutes. The kit is highly efficient for processing small volumes of phage supernatant (500 µL – 1 mL) and with the optional DNase and Proteinase K treatments phage DNA yields are maximized while host DNA contamination is minimized. Purified total phage DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), sequencing, cloning, Southern Blot and more.

Kit Specifications
Column Binding Capacity
50 µg
Maximum Column Loading Volume
650 µg
Size of DNA Purified
All sizes
Maximum Amount of Starting Material
1 x 1010 pfu/mL enriched phages
Average Yield*
3-15 µg DNA from 106-1010 pfu/mL
of enriched phages
Time to Complete 10 Purifications
45 minutes

* Average yields will vary depending upon a number conditions used and developmental stage.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 46800 (50 preps) Cat. 46850 (100 preps)
Lysis Buffer B 40 mL 2 x 40 mL
Wash Solution A 38 mL 2 x 38 mL
Elution Buffer B 8 mL 2 x 8 mL
Spin Columns 50 100
Collection Tubes 50 100
Elution Tubes (1.7 mL) 50 100
Product Insert 1 1

Title Staphylococcus sciuri bacteriophages double-convert for staphylokinase and phospholipase, mediate interspecies plasmid transduction, and package mecA gene
Journal Science Reports. 2017
Authors Zeman M, Mašlaňová I, Indráková A, Šiborová M, Mikulášek K, Bendíčková K, Plevka P, Vrbovská V, Zdráhal Z, Doškař J, Pantůček R
Title Isolation and Characterization of Bacillus cereus Bacteriophages from Foods and Soil
Journal Food and Environmental Virology. 2017
Authors Hyejin Oh, Dong Joo Seo, Su Been Jeon, Hyunkyung Park, Suntak Jeong, Hyang Sook Chun, Mihwa Oh, Changsun Choi
Title Acquisition of Phage Sensitivity by Bacteria through Exchange of Phage Receptors
Journal Cell. 2016.
Authors Tzipilevich E, Habusha M, Ben-Yehuda S.
Title Characterization and in vitro evaluation of new bacteriophages for the biocontrol of Escherichia coli
Journal Virus Research. 2016.
Authors Pereira C, Moreirinha C, Lewicka M, Almeida P, Clemente C, Romalde JL, Nunes ML, Almeida A.
Title Identification of essential genes in the Salmonella phage SPN3US reveals novel insights into giant phage head structure and assembly.
Journal Journal of Virology. 2016.
Authors Thomas JA, Benítez Quintana AD, Bosch MA, Coll De Peña A, Aguilera E, Coulibaly A, Wu W, Osier MV, Hudson AO, Weintraub ST, Black LW
Title The presence of two receptor-binding proteins contributes to the wide host range of staphylococcal Twort-like phages
Journal Applied and Environmental Microbiology. 2016.
Authors Takeuchi I, Osada K, Azam AH, Asakawa H, Miyanaga K, Tanji Y
Title The complete genome sequence and analysis of vB_VorS-PVo5, a Vibrio phage infectious to the pathogenic bacterium Vibrio ordalii ATCC-33509
Journal Standards in Genomic Sciences. 2016.
Authors Echeverría-Vega A, Morales-Vicencio P, Saez-Saavedra C, Ceh J, Araya R
Title Characterization of Five Novel Brevibacillus Bacteriophages and Genomic Comparison of Brevibacillus Phages
Journal PLOS ONE. 2016.
Authors Berg JA, Merrill BD, Crockett JT, Esplin KP, Evans MR, Heaton KE, Hilton JA, Hyde JR, McBride MS, Schouten JT, Simister AR, Thurgood TL, Ward AT, Breakwell DP, Hope S, Grose JH
Title The diversity-generating benefits of a prokaryotic adaptive immune system
Journal Nature. 2016.
Authors van Houte S, Ekroth AK, Broniewski JM, Chabas H, Ashby B, Bondy-Denomy J, Gandon S, Boots M, Paterson S, Buckling A, Westra ER
Title Bacteriophages with potential to inactivate Salmonella Typhimurium: use of single phage suspensions and phage cocktails
Journal Virus Research. 2016.
Authors Pereira C, Moreirinha C, Lewicka M, Almeida P, Clemente C, Cunha Â, Delgadillo I, Romalde JL, Nunes ML, Almeida A
Title A virulent phage JHP against Pseudomonas aeruginosa showed infectivity against multiple genera
Journal Journal of Basic Microbiology. 2016.
Authors Khawaja, K. A., Rauf, M., Abbas, Z
Title Development of an Assay for the Identification of Receptor Binding Proteins from Bacteriophages
Journal Viruses. 2016.
Authors Simpson DJ, Sacher JC, Szymanski CM
Title Isolation and characterization of an Enterococcus faecalis bacteriophage
Journal The Korean Journal of Microbiology. 2015.
Authors Hee-Young Kang, Shukho Kim, Jungmin Kim
Title Complete Genome Sequence of Enterococcus Bacteriophage EFLK1
Journal Genome Announcements. 2015.
Authors Leron Khalifa, Shunit Coppenhagen-Glazer, Mor Shlezinger, Miriam Kott-Gutkowski, Omri Adini, Nurit Beyth, Ronen Hazan
Title Genome Sequences of Five Additional Brevibacillus laterosporus Bacteriophages
Journal Genome Announcements. 2015.
Authors Bryan D. Merrill, Jordan A. Berg, Kiel A. Graves, Andy T. Ward, Jared A. Hilton, Braden N. Wake, Julianne H. Grose, Donald P. Breakwell, Sandra H. Burnett
Title Sewage as a rich source of phage study against Pseudomonas aeruginosa PAO
Journal Biologicals. 2015.
Authors Reza Azizian, Ahmad Nasser, Hasan Askari, Morovat Taheri Kalani, Nourkhoda Sadeghifard, Iraj Pakzad, Razieh Amini, Amir Sasan Mozaffari Nejad, Farid Azizi Jalilian
Title Comparative analysis of multiple inducible phages from Mannheimia haemolytica
Journal BMC Microbiology. 2015.
Authors Yan D. Niu, Shaun R. Cook, Jiaying Wang, Cassidy L. Klima, Yu-hung Hsu, Andrew M. Kropinski, Dann Turner and Tim A. McAllister
Title Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9
Journal mBio. 2015.
Authors Alexandra L. Bryson, Young Hwang, Scott Sherrill-Mix, Gary D. Wu, James D. Lewis, Lindsay Black, Tyson A. Clark, Frederic D. Bushman
Title Characterization of a novel bacteriophage, Phda1, infecting the histamine-producing Photobacterium damselae subsp. damselae
Journal Journal of Applied Microbiology. 2015.
Authors Shogo Yamaki, Yuji Kawai andKoji Yamazaki.
Title A metagenomic approach to characterize temperate bacteriophage populations from Cystic Fibrosis and non-Cystic Fibrosis bronchiectasis patients.
Journal Frontiers in Microbiology. 2015.
Authors Mohammad Adnan Tariq, Francesca Louise Claire Everest, Lauren Cowley, Anthony De_Soyza, Giles Samuel Holt, Simon Harwood Bridge, Audrey Perry, John D Perry, Stephen Bourke, Stephen Cummings, Clare Veronica Lanyon, Jeremy J Barr and Darren Lee Smith.
Title Phage-Encoded Colanic Acid-Degrading Enzyme Permits Lytic Phage Infection of a Capsule-Forming Resistant Mutant Escherichia coli Strain.
Journal Applied and Environmental Microbiology. 2015.
Authors Min Soo Kim, Young Deuk Kim, Sung Sik Hong, Kwangseo Park, Kwan Soo Ko and Heejoon Myung.
Title Evidence That the Heterogeneity of a T4 Population Is the Result of Heritable Traits.
Journal PLoS ONE. 2014.
Authors Zachary J. Storms, Dominic Sauvageau.
Title Phage Encoded Colanic Acid-Degrading Enzyme Permits Lytic Phage Infection of a Capsule-Forming Resistant Mutant Escherichia coli Strain.
Journal Applied and Environmental Microbiology. 2014.
Authors Min Soo Kim, Young Deuk Kim, Sung Sik Hong, Kwangseo Park, Kwan Soo Ko and Heejoon Myung.
Title Complete Genome Sequence of Erwinia amylovora Bacteriophage vB_EamM_Ea35-70.
Journal Genome Announcements. 2014.
Authors Abdelbaset I. Yagubi, Alan J. Castle, Andrew M. Kropinski, Travis W. Banks, Antonet M. Svircev.
Title Characterization of a novel Morganella morganii bacteriophage FSP1 isolated from river water.
Journal FEMS Microbiology Letters. 2014.
Authors Shogo Yamaki, Takuo Omachi, Yuji Kawai andKoji Yamazaki.
Title Characterization and genome sequence of Dev2, a new T7-like bacteriophage infecting Cronobacter turicensis.
Journal Archives of Virology. 2014.
Authors Michal Kajsík, Lucia Oslanecová, Tomáš Szemes, Michalea Hýblová, Andrea Bilková, Hana Drahovská, Ján Turňa.
Title Isolation and characterization of a novel bacteriophage against Mycobacterium avium subspecies paratuberculosis.
Journal Archives of Virology. 2014.
Authors Basra S, Anany H, Brovko L, Kropinski AM, Griffiths MW.
Title Investigation of Mannheimia haemolytica bacteriophages relative to host diversity.
Journal Journal of Applied Microbiology. 2013.
Authors Hsu YH, Cook SR, Alexander TW, Klima CL, Niu YD, Brent Selinger L, McAllister TA.
Title Propionibacterium acnes Bacteriophages Display Limited Genetic Diversity and Broad Killing Activity against Bacterial Skin Isolates.
Journal MBio. 2012
Authors Marinelli LJ, Fitz-Gibbon S, Hayes C, Bowman C, Inkeles M, Loncaric A, Russell DA, Jacobs-Sera D, Cokus S, Pellegrini M, Kim J, Miller JF, Hatfull GF, Modlin RL.
Title Complete Genome of Pseudomonas aeruginosa Phage PA26.
Journal Journal of Virology. 2012
Authors Kim MS, Cha KE, Myung H.
Title Complete Genome of Staphylococcus aureus Phage SA11.
Journal Journal of Virology. 2012
Authors Kim MS, Myung H.
Title Complete Genome Sequence of Caulobacter crescentus Bacteriophage φCbK.
Journal Journal of Virology. 2012
Authors Panis G, Lambert C, Viollier PH.