Phage DNA Isolation Kit
For the rapid purification of total DNA from bacteriophages.
For research use only and NOT intended for in vitro diagnostics.
Phage DNA Isolation Kit
For the rapid purification of total DNA from bacteriophages.
Overview
- Isolate high quality DNA from a broad variety of phage strains
- High yields of total DNA
- Fast and easy processing using a rapid spin-column format
- No phenol or chloroform extractions or cesium chloride banding required
- High yields of DNA recovered 3-15 µg DNA from 106-1010 pfu/ mL of enriched phages
This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. The spin-column based procedure is rapid and can be completed in less than 45 minutes. The kit is highly efficient for processing small volumes of phage supernatant (500 µL – 1 mL) and with the optional DNase and Proteinase K treatments phage DNA yields are maximized while host DNA contamination is minimized. Purified total phage DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), sequencing, cloning, Southern Blot and more.
Details
Supporting Data
Kit Specifications
|
|
Column Binding Capacity
|
50 µg
|
Maximum Column Loading Volume
|
650 µL
|
Size of DNA Purified
|
All sizes
|
Maximum Amount of Starting Material |
1 x 1010 pfu/mL enriched phages
|
Average Yield* |
3-15 µg DNA from 106-1010 pfu/mL
of enriched phages |
Time to Complete 10 Purifications |
45 minutes
|
* Average yields will vary depending upon a number conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 46800 (50 preps) | Cat. 46850 (100 preps) |
---|---|---|
Lysis Buffer B | 40 mL | 2 x 40 mL |
Wash Solution A | 38 mL | 2 x 38 mL |
Elution Buffer B | 8 mL | 2 x 8 mL |
Spin Columns | 50 | 100 |
Collection Tubes | 50 | 100 |
Elution Tubes (1.7 mL) | 50 | 100 |
Product Insert | 1 | 1 |