Plasma/Serum Exosome and Free-Circulating RNA Isolation Kits All-in-one system for the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from plasma/serum samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plasma/Serum Exosome and Free-Circulating RNA Isolation Kits All-in-one system for the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from plasma/serum samples Price USD$1,329.00 SKU 59500 Format Mini Midi Maxi Size 50 Preps Quantity Overview Details Documentation Citations Overview Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. Versatile sample input ranges Isolate all sizes of free-circulating RNA, including microRNA Bind and elute all RNA irrespective of size or GC content, without bias The purified exosomal RNA is free from any circulating RNA-binding proteins No phenol extractions, Proteinase K treatment, nor carrier RNA No time-consuming ultracentrifugation, filtration nor special syringes are required No precipitation reagents, nor overnight incubation required Concentrate isolated exosomal RNA and are free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix These kits provide a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA as well as all sizes of the free-circulating protein-bound RNA, including microRNA. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, these kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit For sample volumes ranging from 50 µL to 1 mL. Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit For sample volumes ranging from 1 mL to 4 mL. Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit For sample volumes ranging from 4 mL to 10 mL. Details Supporting Data Previous Figure 1. Isolation of RNA from exosomes purified from different plasma volumes. Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit (Cat# 59500) was used to isolate Exosomal RNA from different plasma volumes ranging from 0.1 mL and up to 1 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma exosomal miR-26a. (A) The plasma exosomal miR-26a is linearly decreasing with increasing the sample input volume. B) The relative amount of the plasma exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%. Figure 2. Isolation of Free-Circulating RNA after the isolation of Exosomal RNA from different plasma volumes. Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit (Cat# 59500) was used to isolate Free-Circulating RNA after the isolation of Exosomal RNA from different plasma volumes ranging from 0.1 mL and up to 1 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma free-circulating miR-21. (A) The free-circulating miR-21 is linearly decreasing with increasing the sample input volume. B) The relative amount of the free-circulating miR-21 shows excellent linearity with a percentage of recovery of more than 90%. Figure 3. Isolation of RNA from exosomes purified from different plasma volumes. Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit (Cat# 59600) was used to isolate Exosomal RNA from different plasma volumes ranging from 1 mL and up to 4 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma exosomal miR-26a. (A) The plasma exosomal miR-26a is linearly decreasing with increasing the sample input volume. B) The relative amount of the plasma exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%. Figure 4. Isolation of Free-Circulating RNA after the isolation of Exosomal RNA from different plasma volumes. Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit (Cat# 59600) was used to isolate Free-Circulating RNA after the isolation of Exosomal RNA from different plasma volumes ranging from 1 mL and up to 4 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma free-circulating miR-21. (A) The free-circulating miR-21 is linearly decreasing with increasing the sample input volume. B) The relative amount of the free-circulating miR-21 shows excellent linearity with a percentage of recovery of more than 90%. Figure 5. Isolation of RNA from exosomes purified from different plasma volumes. Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit (Cat# 59700) was used to isolate exosomal RNA from different plasma volumes ranging from 4 mL and up to 10 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma exosomal miR-26a. (A) The plasma exosomal miR-26a is linearly decreasing with increasing the sample input volume. B)The relative amount of the plasma exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%. Figure 6. Isolation of Free-Circulating RNA after the isolation of Exosomal RNA from different plasma volumes. Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit (Cat# 59700) was used to isolate free-circulating RNA after the isolation of exosomal RNA from different plasma volumes ranging from 4 mL and up to 10 mL. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma free-circulating miR-21. (A) The free-circulating miR-21 is linearly decreasing with increasing the sample input volume. B) The relative amount of the free-circulating miR-21 shows excellent linearity with a percentage of recovery of more than 90%. Next Click for expanded view Kit Specifications Minimum Plasma Input 50 μL Maximum Plasma Input 1 mL Size of RNA Purified All sizes, including miRNA and small RNA (< 200 nt) Elution Volume 50 - 100 µL Time to Complete 10 Purifications 35 - 40 minutes Average Yields* Variable depending on specimen *Please check page 5 of the product insert for the average yields and the common RNA quantification methods Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. Important Note This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR. Component Cat. 59500 (50 preps) Cat. 59600 (25 preps) Cat. 59700 (15 preps) Slurry E 12.5 mL 12.5 mL 12.5 mL ExoC Buffer 8 mL 8 mL 2 x 8 mL ExoR Buffer 12 mL 12 mL 12 mL Lysis Buffer A 2 x 20 mL 2 x 20 mL 2 x 20 mL Lysis Additive B 2 mL 2 mL 2 mL Wash Solution A 2 x 18 mL 18 mL 18 mL Elution Solution A 2 x 6 mL 6 mL 6 mL Mini Filter Spin Columns 50 25 15 Mini Spin Columns 100 50 30 Collection Tubes 100 50 30 Elution Tubes (1.7 mL) 100 50 30 Product Insert 1 1 1 Documentation PROTOCOLS (59500) Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit - Protocol (50 prep) (59600) Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit - Protocol (25 prep) (59700) Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit - Protocol (15 prep) SAFETY DATA SHEETS (59500, 59600, 59700) Plasma/Serum Exosome and Free-Circulating RNA Isolation Kit - Safety Data Sheet POSTERS RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class Citations Powered by Bioz See more details on Bioz Related Services NGS RNA Isolation Related Products Plasma/Serum Exosome Purification Mini Kit Plasma/Serum Exosome Purification and RNA Isolation Mini Kit Exosomal RNA Isolation Kit
Plasma/Serum Exosome and Free-Circulating RNA Isolation Kits All-in-one system for the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from plasma/serum samples For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Plasma/Serum Exosome and Free-Circulating RNA Isolation Kits All-in-one system for the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from plasma/serum samples Price USD$1,329.00 SKU 59500 Format Mini Midi Maxi Size 50 Preps Quantity