Plasma/Serum Exosome Purification and RNA Isolation Kits

Cat. 58300, 58500, 58600
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*Inquire by phone or email for more information.

New Application Note

Norgen's Plasma/Serum Exosome Purification and RNA Isolation Kits can be used with heparinized plasma.

Application Note 99: Small RNA Sequencing of Plasma Exosomes Collected on Heparin: Quality and Quantity Challenges

Plasma/Serum Exosome Purification and RNA Isolation Kits

Cat. 58300, 58500, 58600

All-in-one system for the purification of exosomes and the subsequent isolation of RNA from plasma/serum samples

  • Purification and enrichment of intact plasma/serum exosomes for functional studies
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
  • Versatile plasma/serum input volume
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents, nor overnight incubation required
  • Compatible with plasma/serum from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Plasma/Serum Exosome Purification and RNA Isolation Mini Kit
(Cat. 58300)
50 preps
Plasma/Serum Exosome Purification and RNA Isolation Midi Kit
(Cat. 58500)
25 preps
Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit
(Cat. 58600)
15 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Purification and enrichment of intact plasma/serum exosomes for functional studies
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
  • Versatile plasma/serum input volume
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents, nor overnight incubation required
  • Compatible with plasma/serum from any species
  • Pure exosomes are purified and are free-from any other RNA-binding proteins
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Plasma/Serum Exosome Purification and RNA Isolation Kits

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the sequential isolation of exosomal RNA and free-circulating RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit

For sample volumes ranging from 50 µL to 1 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit

For sample volumes ranging from 1 mL to 4 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit

For sample volumes ranging from 4 mL to 10 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Kit Specifications

You have selected: Cat. 58300
Kit Specifications
Minimum Plasma/Serum Input
50 μL
Maximum Plasma/Serum Input
1 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

You have selected: Cat. 58500
Kit Specifications
Minimum Plasma/Serum Input
1 mL
Maximum Plasma/Serum Input
4 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

You have selected: Cat. 58600
Kit Specifications
Minimum Plasma/Serum Input
4 mL
Maximum Plasma/Serum Input
10 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Supporting Data

Click for expanded view

Component Cat. 58300 (50 preps) Cat. 58500 (25 preps) Cat. 58600 (15 preps)
Slurry E 12.5 mL 12.5 mL 12.5 mL
ExoC Buffer 8 mL 8 mL 2 x 8 mL
ExoR Buffer 12 mL 12 mL 12 mL
Lysis Buffer A 20 mL 20 mL 20 mL
Lysis Additive B 2 mL 2 mL 2 mL
Wash Solution A 38 mL 18 mL 18 mL
Elution Solution A 6 mL 6 mL 6 mL
Mini Filter Spin Columns 50 25 15
Mini Spin Columns 50 25 15
Collection Tubes 50 25 15
Elution Tubes (1.7 mL) 100 50 30
Product Insert 1 1 1

Mini (58300)

Title Cancer cells exploit an orphan RNA to drive metastatic progression
Journal Nature Medicine. 2018.
Authors Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi

Maxi (58500)

Plasma/Serum Exosome Purification and RNA Isolation Kits

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the sequential isolation of exosomal RNA and free-circulating RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit

For sample volumes ranging from 50 µL to 1 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit

For sample volumes ranging from 1 mL to 4 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit

For sample volumes ranging from 4 mL to 10 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 58300
Kit Specifications
Minimum Plasma/Serum Input
50 μL
Maximum Plasma/Serum Input
1 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

You have selected: Cat. 58500
Kit Specifications
Minimum Plasma/Serum Input
1 mL
Maximum Plasma/Serum Input
4 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

You have selected: Cat. 58600
Kit Specifications
Minimum Plasma/Serum Input
4 mL
Maximum Plasma/Serum Input
10 mL
Size of Exosomes Purified
40 nm - 150 nm
Size of RNA Purified All sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume 50-100 μL
Time to Complete 10 Purifications
35 - 40 minutes
Average Yields* Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Component Cat. 58300 (50 preps) Cat. 58500 (25 preps) Cat. 58600 (15 preps)
Slurry E 12.5 mL 12.5 mL 12.5 mL
ExoC Buffer 8 mL 8 mL 2 x 8 mL
ExoR Buffer 12 mL 12 mL 12 mL
Lysis Buffer A 20 mL 20 mL 20 mL
Lysis Additive B 2 mL 2 mL 2 mL
Wash Solution A 38 mL 18 mL 18 mL
Elution Solution A 6 mL 6 mL 6 mL
Mini Filter Spin Columns 50 25 15
Mini Spin Columns 50 25 15
Collection Tubes 50 25 15
Elution Tubes (1.7 mL) 100 50 30
Product Insert 1 1 1

Mini (58300)

Title Cancer cells exploit an orphan RNA to drive metastatic progression
Journal Nature Medicine. 2018.
Authors Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi

Maxi (58500)

 


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