Plasma/Serum Exosome Purification and RNA Isolation Kits

Cat. 58300, 58500, 58600

Plasma/Serum Exosome Purification and RNA Isolation Mini Kit

Plasma/Serum Exosome Purification and RNA Isolation Midi Kit

Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit

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*Inquire by phone or email for more information.

New Application Note

Norgen's Plasma/Serum Exosome Purification and RNA Isolation Kits can be used with heparinized plasma.

Application Note 99: Small RNA Sequencing of Plasma Exosomes Collected on Heparin: Quality and Quantity Challenges

Plasma/Serum Exosome Purification and RNA Isolation Kits

Cat. 58300, 58500, 58600

All-in-one system for the purification of exosomes and the subsequent isolation of RNA from plasma/serum samples

Purification and enrichment of intact plasma/serum exosomes for functional studies
Bind and elute all RNA irrespective of size or GC content, without bias
Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
Versatile plasma/serum input volume
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents, nor overnight incubation required
Compatible with plasma/serum from any species
Pure exosomes are purified and are free-from any other RNA-binding proteins
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services

For research use only and NOT intended for in vitro diagnostics.

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Plasma/Serum Exosome Purification and RNA Isolation Mini Kit
(Cat. 58300) 50 preps

Plasma/Serum Exosome Purification and RNA Isolation Midi Kit
(Cat. 58500) 25 preps

Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit
(Cat. 58600) 15 preps

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Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. 58300

Kit Specifications
Minimum Plasma/Serum Input	50 μL
Maximum Plasma/Serum Input	1 mL
Size of Exosomes Purified	40 nm - 150 nm
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35 - 40 minutes
Average Yields*	Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

You have selected: Cat. 58500

Kit Specifications
Minimum Plasma/Serum Input	1 mL
Maximum Plasma/Serum Input	4 mL
Size of Exosomes Purified	40 nm - 150 nm
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35 - 40 minutes
Average Yields	Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods

You have selected: Cat. 58600

Kit Specifications
Minimum Plasma/Serum Input	4 mL
Maximum Plasma/Serum Input	10 mL
Size of Exosomes Purified	40 nm - 150 nm
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35 - 40 minutes
Average Yields*	Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods

Supporting Data

Figure 1. Isolation of RNA from exosomes purified from different plasma volumes. Norgen's Plasma/Serum Exosome Purification and RNA Isolation Mini Kit (Cat# 58300) was used to isolate RNA from exosomes purified from different plasma volumes using the same kit. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma exosomal miR-26a. (A) The plasma exosomal miR-26a is linearly decreasing with increasing the sample input volume. (B) The relative amount of the plasma exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%.

Figure 2. Intact exosomes were purified from 1 mL plasma using different purification methods.Intact exosomes were purified from 1 mL plasma using Norgen's Plasma/Serum Exosome Purification and RNA Isolation Mini Kit (Cat# 58300), Competitor S's kit and Competitor L's kit (from plasma). Exosomes purified using Norgen's kit were resuspended in 200 µL of Norgen's ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that Norgen's kit isolated 55 nm exosomes with a recovery of 9.64 x 10¹³ particles/mL plasma. No impurities were found to be contaminating the exosomes purified using Norgen's Plasma/Serum Exosome Purification and RNA Isolation Kit. Additionally, exosomes with a broader size range covering from 50 nm - 150 nm were purified from 1 mL plasma with a higher concentration compared to the other two methods.

Figure 3. Intact exosomes purified from 1 mL and 10 mL plasma. Intact exosomes were purified from 1 mL plasma using Norgen's Plasma/Serum Exosome Purification and RNA Isolation Mini Kit (Cat# 58300) and from 10 mL plasma using Norgen's Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit (Cat# 58600). Exosomes purified using Norgen's mini kit were resuspended in 200 µL of Norgen's ExoR buffer whereas exosomes purified using Norgen's Maxi kit were resuspended in 600 µL Norgen's ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that the purification of exosomes is linear as 4.04 x 10¹⁰ particles/mL were recovered from 1 mL plasma, whereas 2.95 x 10¹¹ particles/mL were recovered from 10 mL plasma.

Figure 4. Isolation of RNA from exosomes purified from different plasma volumes. Norgens Plasma/Serum Exosome Purification and RNA Isolation Midi Kit (Cat# 58500) was used to isolate RNA from exosomes purified from different plasma volumes using the same kit. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma exosomal miR-26a. (A) The plasma exosomal miR-26a is linearly decreasing with increasing sample input volume. B) The relative amount of the plasma exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%.

Figure 5. Intact exosomes were purified from 1 mL plasma using different purification methods. Intact exosomes were purified from 1 mL plasma using Norgens Plasma/Serum Exosome Purification and RNA Isolation Mini Kit (Cat# 58300), Competitor S's kit and Competitor L's kit (from plasma). Exosomes purified using Norgens kit were resuspended in 200 µL of Norgens ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that Norgens kit isolated 55 nm exosomes with a recovery of 9.64 x 10^13 particles/mL plasma. No impurities were found to be contaminating the exosomes purified using Norgens Plasma/Serum Exosome Purification and RNA Isolation Mini Kit. Additionally, exosomes with a broader size range covering from 50 nm - 150 nm was purified from 1 mL plasma with a higher concentration compared to the other two methods.

Figure 6. Intact exosomes purified from 1 mL and 10 mL plasma. Intact exosomes were purified from 1 mL plasma using Norgens Plasma/Serum Exosome Purification and RNA Isolation Mini Kit (Cat# 58300) and from 10 mL plasma using Norgen's Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit (Cat# 58600). Exosomes purified using Norgens mini kit were resuspended in 200 µL of Norgens ExoR buffer, whereas exosomes purified using Norgens Maxi kit were resuspended in 600 µL Norgens ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that the purification of exosomes is linear as 4.04 x 10^10 particles/mL were recovered from 1 mL plasma whereas 2.95 x 10^11 particles/mL were recovered from 10 mL plasma.

Figure 7. Isolation of RNA from exosomes purified from different plasma volumes. Norgens Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit (Cat# 58600) was used to isolate RNA from exosomes purified from different plasma volumes using the same kit. Two microlitres of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma exosomal miR-26a. (A) The plasma exosomal miR-26a is linearly decreasing with increasing sample input volume. B) The relative amount of the plasma exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%.

Figure 8. Intact exosomes were purified from 1 mL plasma using different purification methods. Intact exosomes were purified from 1 mL plasma using Norgens Plasma/Serum Exosome Purification and RNA Isolation Mini Kit (Cat# 58300), Competitor Ss kit and Competitor Ls kit (from plasma). Exosomes purified using Norgens kit was resuspended in 200 µL Norgens ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that Norgens kit isolated 55 nm exosomes with a recovery of 9.64 x 10^13 particles/mL plasma. No impurities were found to be contaminating the exosomes purified using Norgens Plasma/Serum Exosome Purification and RNA Isolation Mini Kit. Additionally, exosomes with a broader size range covering from 50 nm 150 nm was purified from 1 mL plasma with a higher concentration compared to the other two methods.

Figure 9. Intact exosomes purified from 1 mL and 10 mL plasma. Intact exosomes were purified from 1 mL plasma using Norgens Plasma/Serum Exosome Purification and RNA Isolation Mini Kit (Cat# 58300) and from 10 mL plasma using the Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit (Cat#56800). Exosomes purified using Norgens Mini kit were resuspended in 200 µL Norgens ExoR buffer whereas exosomes purified using Norgens Maxi kit were resuspended in 600 µL Norgens ExoR buffer, diluted 1:1,000 and visualized on the NanoSight LM10 instrument. The analysis shows that the purification of exosomes is linear as 4.04 x 10^10 particles/mL were recovered from 1 mL plasma whereas 2.95 x 10^11 particles/mL were recovered from 10 mL plasma.

Click for expanded view

Protocols:

(58300) Plasma/Serum Exosome Purification and RNA Isolation Mini Kit - Protocol (50 prep)

(58500) Plasma/Serum Exosome Purification and RNA Isolation Midi Kit - Protocol (25 prep)

(58600) Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit - Protocol (15 prep)

Application Notes:

Small RNA Sequencing of Plasma Exosomes Collected on Heparin: Quality and Quantity Challenges

Safety Data Sheets:

(58300, 58500, 58600) Plasma/Serum Exosome Purification and RNA Isolation Kit - Safety Data Sheet

Product Flyers:

Exosome Purification

Mini (58300)

Title	Cancer cells exploit an orphan RNA to drive metastatic progression
Journal	Nature Medicine. 2018.
Authors	Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi

Maxi (58500)

Title	Small RNA-sequence analysis of plasma-derived extracellular vesicle miRNAs in smokers and patients with chronic obstructive pulmonary disease as circulating biomarkers
Journal	Journal of Extracellular Vesicles, 8(1). 2019.
Authors	Sundar, I. K., Li, D., & Rahman, I.

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Plasma/Serum Exosome Purification and RNA Isolation Kits

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the sequential isolation of exosomal RNA and free-circulating RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit

For sample volumes ranging from 50 µL to 1 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Midi Kit

For sample volumes ranging from 1 mL to 4 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit

For sample volumes ranging from 4 mL to 10 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 58300

Kit Specifications
Minimum Plasma/Serum Input	50 μL
Maximum Plasma/Serum Input	1 mL
Size of Exosomes Purified	40 nm - 150 nm
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35 - 40 minutes
Average Yields*	Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

You have selected: Cat. 58500

Kit Specifications
Minimum Plasma/Serum Input	1 mL
Maximum Plasma/Serum Input	4 mL
Size of Exosomes Purified	40 nm - 150 nm
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35 - 40 minutes
Average Yields	Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods

You have selected: Cat. 58600

Kit Specifications
Minimum Plasma/Serum Input	4 mL
Maximum Plasma/Serum Input	10 mL
Size of Exosomes Purified	40 nm - 150 nm
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35 - 40 minutes
Average Yields*	Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods

Protocols:

(58300) Plasma/Serum Exosome Purification and RNA Isolation Mini Kit - Protocol (50 prep)

(58500) Plasma/Serum Exosome Purification and RNA Isolation Midi Kit - Protocol (25 prep)

(58600) Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit - Protocol (15 prep)

Application Notes:

Small RNA Sequencing of Plasma Exosomes Collected on Heparin: Quality and Quantity Challenges

Safety Data Sheets:

(58300, 58500, 58600) Plasma/Serum Exosome Purification and RNA Isolation Kit - Safety Data Sheet

Product Flyers:

Exosome Purification

Mini (58300)

Title	Cancer cells exploit an orphan RNA to drive metastatic progression
Journal	Nature Medicine. 2018.
Authors	Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi

Maxi (58500)

Title	Small RNA-sequence analysis of plasma-derived extracellular vesicle miRNAs in smokers and patients with chronic obstructive pulmonary disease as circulating biomarkers
Journal	Journal of Extracellular Vesicles, 8(1). 2019.
Authors	Sundar, I. K., Li, D., & Rahman, I.

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Plasma/Serum Exosome Purification and RNA Isolation Kits (Cat. 58300, 58500, 58600)

Plasma/Serum Exosome Purification and RNA Isolation Kits

New Application Note

Plasma/Serum Exosome Purification and RNA Isolation Kits

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Related Products

Exosomal RNA Isolation Kit

Plasma/Serum Exosome Purification Kit

Plasma/Serum Exosome Purification and RNA Isolation Kits (Cat. 58300, 58500, 58600)

Plasma/Serum Exosome Purification and RNA Isolation Kits

New Application Note

Plasma/Serum Exosome Purification and RNA Isolation Kits

Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Product Overview

Documentation

Citations

Related Products

Exosomal RNA Isolation Kit

Plasma/Serum Exosome Purification Kit

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.