|Plasma/Serum Exosome Purification and RNA Isolation Mini Kit||58300||50 preps|
Plasma/Serum Exosome Purification and RNA Isolation Mini Kit
Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the sequential isolation of exosomal RNA and free-circulating RNA from different plasma/serum sample volumes ranging from 50 µL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
|Minimum Plasma/Serum Input||
Maximum Plasma/Serum Input
Size of Exosomes Purified
40 nm - 150 nm
|Size of RNA Purified||All sizes, including miRNA
and small RNA (< 200 nt)
|Elution Volume||50-100 μL|
|Time to Complete 10 Purifications||
35 - 40 minutes
|Average Yields*||Variable depending on specimen|
*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 years without showing any reduction in performance.
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
|Title||Cancer cells exploit an orphan RNA to drive metastatic progression|
|Journal||Nature Medicine. 2018.|
|Authors||Lisa Fish, Steven Zhang, Johnny X. Yu, Bruce Culbertson, Alicia Y. Zhou, Andrei Goga & Hani Goodarzi|
Copyright © 2019 - Norgen Biotek Corp.