RNase-Free DNase I Kit

Features and Benefits

Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits
Also includes protocol for digestion in-solution followed by RNA Clean-Up
Guaranteed RNase-Free
Includes Enzyme Incubation Buffer
Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600 units/vial)

Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.

Details

Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.

Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.

Component	Cat. 25710 (50 rxns)	Cat. 25720 (200 rxns)
DNase I	1 vial / 1,600 units	4 vials (1,600 units/vial)
Buffer DR	1 mL	4 x 1 mL
Enzyme Incubation Buffer	6 mL	4 x 6 mL
Product Insert	1	1

Documentation

PROTOCOLS

(25710) RNase-Free DNase I Kit - Protocol (50 reactions)

(25720) RNase-Free DNase I Kit - Protocol (200 reactions)

SAFETY DATA SHEETS

25710 - RNase-Free DNase I Kit - SDS

25720 - RNase-Free DNase I Kit - SDS

Citations

Title	Possibilities for reproduction and archiving resulting in a clone collection of a unique grey poplar (Populus × canescens Aiton Sm.) population in the Czech Republic
Citation	Central European Forestry Journal 2024.
Authors	Eva Pokorná*, Ludka Cížková, Pavlína Máchová, Martina Komárková, Helena Cvrcková, Jaroslav Dostál

Title	Sterile inflammation via TRPM8 RNA-dependent TLR3-NF-kB/IRF3 activation promotes antitumor immunity in prostate cancer
Citation	The EMBO Journal 2024.
Authors	Alessandro Alaimo 1,17?, Sacha Genovesi 1 , Nicole Annesi1 , Dario De Felice 1 , Saurav Subedi2, Alice Macchia1 , Federico La Manna2 , Yari Ciani1 , Federico Vannuccini 1 , Vera Mugoni1 Michela Notarangelo 1 , Michela Libergoli 1 , Francesca Broso 1 , Riccardo Taulli3,4, Ugo Ala 5 Aurora Savino6 , Martina Cortese 1 , Somayeh Mirzaaghaei 6,7, Valeria Poli 6,7, Ian Marc Bonapace8 Mauro Giulio Papotti9 , Luca Molinaro9 , Claudio Doglioni10, Orazio Caffo11, Adriano Anesi12, Michael Nagler13, Giovanni Bertalot 14,15, Francesco Giuseppe Carbone14, Mattia Barbareschi 14,15, Umberto Basso16, Erik Dassi1 , Massimo Pizzato1 , Alessandro Romanel 1 , Francesca Demichelis1 Marianna Kruithof-de Julio2,13 & Andrea Lunardi

Title	A multiple super-enhancer region establishes inter-TAD interactions and controls Hoxa function in cranial neural crest
Citation	nature communications 2023.
Authors	Sandra Kessler, Maryline Minoux, Onkar Joshi, Yousra Ben Zouari, Sebastien Ducret, Fiona Ross, Nathalie Vilain, Adwait Salvi, Joachim Wolff, Hubertus Kohler, Michael B. Stadler & Filippo M. Rijli

Title	EBV infection of primary colonic epithelial cells causes inflammation, DDR and autophagy dysregulation, effects that may predispose to IBD and carcinogenesis
Citation	Virus Research 2023.
Authors	Roberta Santarelli, Lorenzo Evangelista, Chiara Pompili, Salvatore Lo Presti, Alberto Rossi, Andrea Arena, Aurelia Gaeta, Roberta Gonnella, Maria Saveria Gilardini Montani, Mara Cirone

Title	HHV-6A Infection of Papillary Thyroid Cancer Cells Induces Several Effects Related to Cancer Progression
Citation	Viruses 2023.
Authors	Stefania Mardente, Maria Anele Romeo, Angela Asquino, Agnese Po, Maria Saveria Gilardini Montani and Mara Cirone

Title	Potential diagnostic value of N1LR and SNHG1 in acute myocardial infarction
Citation	BMC Medical Genomics 2023.
Authors	Wei Zhu, Li Luo, Guangning Ye & Jiaman Ou

Title	Promoter insertion leads to polyembryony in mango — a case of convergent evolution with citrus
Citation	Horticulture Research 2023.
Authors	Chandra Bhan Yadav, Ada Rozen, Ravit Eshed, Mazal Ish-Shalom, Adi Faigenboim, Natalie Dillon, Ian Bally, Matthew Webb, David Kuhn, Ron Ophir, Yuval Cohen, Amir Sherman

Title	RNA sequencing of early round goby embryos reveals that maternal experiences can shape the maternal RNA contribution in a wild vertebrate
Citation	BMC Evolutionary Biology 2018.
Authors	Adrian-Kalchhauser, I., Walser, J. C., Schwaiger, M., & Burkhardt-Holm, P. (2018).

Title	Cell-Free Urinary MicroRNAs Expression in Small-Scale Experiments
Citation	MicroRNA Detection and Target Identification 2017.
Authors	Zavesky, L., Jandakova, E., Turyna, R., Duskova, D., Langmeierova, L., Weinberger, V., ... & Kohoutova, M. (2017)

Title	Early onset of inflammation during ontogeny of bipolar disorder: the NLRP2 inflammasome gene distinctly differentiates between patients and healthy controls in the transition between iPS cell and neural stem cell stages
Citation	Translational Psychiatry 2017.
Authors	Vizlin-Hodzic, D., Zhai, Q., Illes, S., Södersten, K., Truvé, K., Parris, T. Z., ... & Strandberg, J