For rapid and efficient clean-up and concentration of total RNA, including microRNA, without phenol

  • Clean-up & concentrate total RNA (including miRNA) in minutes
  • Clean-up & concentrate RNA from TRIzol®, TRI Reagent®, etc
  • Clean-up RNA from contaminants including enzymes, primers, nucleotides
  • Rapid spin-column protocol, elute in 20 µL
  • 96-well format for high throughput processing & small spin column formats for 8 µL are available
  • Purified RNA is fully compatible with all downstream applications

ItemsCat. #Size
RNA Clean-Up and Concentration Kit 23600 50 preps
RNA Clean-Up and Concentration Kit 43200 100 Preps
RNA Clean-Up and Concentration 96-Well Kit 25100 2 x 96-well plates

RNA Clean-Up and Concentration Kit

This kit is designed to clean-up and concentrate RNA (including miRNA) from TRIzol® and TRI Reagent®, enzymatic reactions, in vitro transcription, labelling reactions, etc. This is a robust kit for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications.

The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.

The kit elutes RNA in 20 µL for all types of downstream applications. For smaller elution volumes of 8 µL, please refer to the microspin column kits such as the RNA Clean-Up and Concentration Micro-Elute Kit for the highest concentration of RNA sample.

Protocol

Kit Specifications (Spin Column)
Maximum Column Binding Capacity 35 μg
Size of RNA Purified All sizes, including small RNA
(<200 nt)
Maximum Amount of starting Material 35 μg of RNA
Minimum Elution Volume 20 μL
Time to Complete 10 Purifications 20 minutes
Average Recovery ≥ 90%
Kit Specifications (96-well plate)
Column Binding Capacity 50 μg
Size of RNA Purified All sizes, including small RNA
(< 200 nt)
Maximum Amount of Starting Material 50 μg of RNA
Minimum Elution Volume 75 μL
Time to Complete 10 Purifications 30 minutes
Average Recovery ≥ 90%

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.


Kit Components
Component Cat. 23600 (50 preps) Cat. 43200 (100 preps)
Buffer RL 40 mL 2 x 40 mL
Wash Solution A 38 mL 2 x 38 mL
Elution Solution A 6 mL 2 x 6 mL
Micro Spin Columns 50 100
Collection Tubes 50 100
Elution Tubes (1.7 mL) 50 100
Product Insert 1 1
Component Cat. 25100 (96-well)
Buffer RL 2 x 40 mL
Wash Solution A 2 x 38 mL
Elution Solution A 2 x 20 mL
96-Well Plate 2
Adhesive Tape 4
96-Well Collection Plate 2
96-Well Elution Plate 2
Product Insert 1

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