RNase-Free DNase I Kit
For DNase digestion during RNA purification either on-column or in-solution
For research use only and NOT intended for in vitro diagnostics.
RNase-Free DNase I Kit
For DNase digestion during RNA purification either on-column or in-solution
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Features and Benefits
- Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits
- Also includes protocol for digestion in-solution followed by RNA Clean-Up
- Guaranteed RNase-Free
- Includes Enzyme Incubation Buffer
- Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600 units/vial)
Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Details
Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.
Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.
Component | Cat. 25710 (50 rxns) | Cat. 25720 (200 rxns) |
---|---|---|
DNase I | 1 vial / 1,600 units | 4 vials (1,600 units/vial) |
Buffer DR | 1 mL | 4 x 1 mL |
Enzyme Incubation Buffer | 6 mL | 4 x 6 mL |
Product Insert | 1 | 1 |
Documentation
Citations
Title | Selection of Suitable Internal Control Genes for Accurate Normalization of Real-Time Quantitative PCR Data of Buffalo (Bubalus bubalis) Blastocysts Produced by SCNT and IVF |
Citation | Cellular Reprogramming 2017. |
Authors | Sood, T. J., Lagah, S. V., Sharma, A., Singla, S. K., Mukesh, M., Chauhan, M. S., ... & Palta, P. (2017). |
Title | The central anorexigenic mechanism of amylin in Japanese quail (Coturnix japonica) involves pro-opiomelanocortin, calcitonin receptor, and the arcuate nucleus of the hypothalamus |
Citation | Comparative Biochemistry and Physiology 2017. |
Authors | Yuan, J., Gilbert, E. R., & Cline, M. A. (2017). |
Title | Complete Transcriptome RNA-Seq |
Citation | Cancer Gene Networks 2016. |
Authors | Miller, D. F., Yan, P., Fang, F., Buechlein, A., Kroll, K., Frankhouser, D., ... & Michaels, S |
Title | The Response of microRNAs to Solar UVR in Skin-Resident Melanocytes Differs between Melanoma Patients and Healthy Persons |
Citation | PLoS One 2016. |
Authors | Sha J, Gastman BR, Morris N, Mesinkovska NA, Baron ED, Cooper KD, et al. |
Title | the Molecular and Genetic Basis of Repeatable Coevolution between Escherchia coli and Bacteriophage T3 in a Laboratory Microcosm |
Citation | PLoS One 2015. |
Authors | EB Perry, JE Barrick, BJM Bohannan |