For the rapid purification of high-quality DNA from preserved and fresh saliva samples for in vitro diagnostic use

Note: This product is not available for sale in the United States

  • CE-IVD marked in accordance with EU Directive 98/79/EC
  • Ideal for use in in vitro diagnostic workflows
  • Sample collection is non-invasive and painless
  • Fast and easy processing using a rapid spin-column format
  • Isolate high quality genomic DNA to be used in downstream in vitro diagnostic applications
  • Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples

ItemsCat. #Size
Saliva DNA Isolation Kit Dx Dx45400 50 preps

Saliva DNA Isolation Kit Dx

This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. 49000), as well as fresh saliva samples.

Saliva DNA purified using Norgen’s Saliva DNA Isolation Kit Dx kit is of the highest quality, and can be used with any downstream in vitro diagnostic application employing enzymatic amplification or other enzymatic modifications of DNA followed by signal detection or amplification.

Background

Saliva represents an excellent non-invasive alternative to blood collection. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.

NOTE: This product is not available for sale in the United States.

Kit Specifications
Maximum Saliva Input
0.5 mL preserved saliva
0.25 mL fresh saliva
Average Yield from 0.25 mL of Saliva*
3 - 7 μg
Average Purity (OD260/280)
1.7 - 2.1
Time to Complete 10 Purifications
30 minutes

* Average yield will depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels. The Proteinase K can be stored at either room temperature or 4oC.


Kit Components
Component Cat. Dx45400 (50 preps)
Lysis Solution 30 mL
Proteinase K 1.2 mL
Binding Solution 12 mL
Wash Solution 18 mL
Elution Buffer 12 mL
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title The myth of oral hygiene using synthetic mouthwash products
Journal SpringerPlus. 2016.
Authors Ghulam Zahara Jahangir, Durre Shahwar Ashraf, Idrees Ahmad Nasir, Madeha Sadiq, Sobia Shahzad, Farah Naz, Muhammad Iqbal and Afifa Saeed
Title Case-control study of glucocorticoid receptor and corticotrophin-releasing hormone receptor gene variants and risk of perinatal depression
Journal BMC Pregnancy and Childbirth. 2015.
Authors Ene-Choo Tan, Tze-Ern Chua, Theresa M. Y. Lee, Hui-San Tan, Joe L. Y. Ting and Helen Y. Chen
Title First Detection of Antibodies Against African Swine Fever Virus in Faeces Samples
Journal Transboundary and Emerging Diseases. 2015.
Authors E. Nieto-Pelegrın, B. Rivera-Arroyo and J. M. Sanchez-Vizcaıno
Title 1000 Norms Project: protocol of a cross-sectional study cataloging human variation
Journal Physiotherapy. 2015.
Authors Marnee J. McKay, Jennifer N. Baldwin, Paulo Ferreira, Milena Simic, Natalie Vanicek, Claire E. Hiller, Elizabeth J. Nightingale, Niamh A. Moloney, Kate G. Quinlan, Fereshteh Pourkazemi, Amy D. Sman, Leslie L. Nicholson, Seyed J. Mousavi, Kristy Rose, Jacqueline Raymond, Martin G. Mackey, Angus Chard, Markus Hübscher, Caleb Wegener, Alycia Fong Yan, Kathryn M. Refshauge, Joshua Burns
Title TAS2R38 single nucleotide polymorphisms are associated with PROP- but not thermal tasting: a pilot study.
Journal Chemosensory Perception. 2013.
Authors Bering A, Pickering G, Liang P.
Title Brief communication: Evolution of a specific O allele (O1v(G542A) ) supports unique ancestry of Native Americans.
Journal American Journal of Physical Anthropology. 2013.
Authors Villanea FA, Bolnick DA, Monroe C, Worl R, Cambra R, Leventhal A, Kemp BM.