Saliva DNA Isolation Kit Dx (Cat. Dx45400)
Saliva DNA Isolation Kit Dx
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Saliva DNA Isolation Kit Dx
For the rapid purification of high-quality DNA from preserved and fresh saliva samples for in vitro diagnostic use
- CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
- Ideal for use in in vitro diagnostic workflows
- Sample collection is non-invasive and painless
- Fast and easy processing using a rapid spin-column format
- Isolate high quality genomic DNA to be used in downstream in vitro diagnostic applications
- Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples
Note: This product is not available for sale in the United States
Research version available here
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(Cat. Dx45400) 50 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Saliva Input
|
0.5 mL preserved saliva
0.25 mL fresh saliva |
|
Average Yield from 0.25 mL of Saliva*
|
3 - 7 μg
|
|
Average Purity (OD260/280)
|
1.7 - 2.1
|
| Time to Complete 10 Purifications |
30 minutes
|
* Average yield will depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels. The Proteinase K can be stored at either room temperature or 4oC.
Supporting Data
| Component | Cat. Dx45400 (50 preps) |
|---|---|
| Lysis Solution | 30 mL |
| Proteinase K in Storage Buffer | 1.2 mL |
| Binding Solution | 12 mL |
| Wash Solution | 18 mL |
| Elution Buffer | 12 mL |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | Associations between the oxytocin receptor gene (OXTR) rs53576 polymorphism and emotional processing of social and nonsocial cues: an event-related potential (ERP) study |
| Journal | Journal of Physiological Anthropology. 2017. |
| Authors | Choi D, Minote N, Watanuki S. |
| Title | The myth of oral hygiene using synthetic mouthwash products |
| Journal | SpringerPlus. 2016. |
| Authors | Ghulam Zahara Jahangir, Durre Shahwar Ashraf, Idrees Ahmad Nasir, Madeha Sadiq, Sobia Shahzad, Farah Naz, Muhammad Iqbal and Afifa Saeed |
| Title | Case-control study of glucocorticoid receptor and corticotrophin-releasing hormone receptor gene variants and risk of perinatal depression |
| Journal | BMC Pregnancy and Childbirth. 2015. |
| Authors | Ene-Choo Tan, Tze-Ern Chua, Theresa M. Y. Lee, Hui-San Tan, Joe L. Y. Ting and Helen Y. Chen |
| Title | First Detection of Antibodies Against African Swine Fever Virus in Faeces Samples |
| Journal | Transboundary and Emerging Diseases. 2015. |
| Authors | E. Nieto-Pelegrın, B. Rivera-Arroyo and J. M. Sanchez-Vizcaıno |
| Title | 1000 Norms Project: protocol of a cross-sectional study cataloging human variation |
| Journal | Physiotherapy. 2015. |
| Authors | Marnee J. McKay, Jennifer N. Baldwin, Paulo Ferreira, Milena Simic, Natalie Vanicek, Claire E. Hiller, Elizabeth J. Nightingale, Niamh A. Moloney, Kate G. Quinlan, Fereshteh Pourkazemi, Amy D. Sman, Leslie L. Nicholson, Seyed J. Mousavi, Kristy Rose, Jacqueline Raymond, Martin G. Mackey, Angus Chard, Markus Hübscher, Caleb Wegener, Alycia Fong Yan, Kathryn M. Refshauge, Joshua Burns |
| Title | TAS2R38 single nucleotide polymorphisms are associated with PROP- but not thermal tasting: a pilot study. |
| Journal | Chemosensory Perception. 2013. |
| Authors | Bering A, Pickering G, Liang P. |
| Title | Brief communication: Evolution of a specific O allele (O1v(G542A) ) supports unique ancestry of Native Americans. |
| Journal | American Journal of Physical Anthropology. 2013. |
| Authors | Villanea FA, Bolnick DA, Monroe C, Worl R, Cambra R, Leventhal A, Kemp BM. |
| Title | Differences in the quantity of DNA found in the urine and saliva of smokers versus nonsmokers: implications for the timing of epigenetic events. |
| Journal | Epigenomics. 2012. |
| Authors | Simkin M, Abdalla M, El-Mogy M, Haj-Ahmad Y. |
Saliva DNA Isolation Kit Dx
This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. 49000), as well as fresh saliva samples.
Saliva DNA purified using Norgen’s Saliva DNA Isolation Kit Dx kit is of the highest quality, and can be used with any downstream in vitro diagnostic application employing enzymatic amplification or other enzymatic modifications of DNA followed by signal detection or amplification.
Background
Saliva represents an excellent non-invasive alternative to blood collection. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.
NOTE: This product is not available for sale in the United States.
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Saliva Input
|
0.5 mL preserved saliva
0.25 mL fresh saliva |
|
Average Yield from 0.25 mL of Saliva*
|
3 - 7 μg
|
|
Average Purity (OD260/280)
|
1.7 - 2.1
|
| Time to Complete 10 Purifications |
30 minutes
|
* Average yield will depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels. The Proteinase K can be stored at either room temperature or 4oC.
| Component | Cat. Dx45400 (50 preps) |
|---|---|
| Lysis Solution | 30 mL |
| Proteinase K in Storage Buffer | 1.2 mL |
| Binding Solution | 12 mL |
| Wash Solution | 18 mL |
| Elution Buffer | 12 mL |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | Associations between the oxytocin receptor gene (OXTR) rs53576 polymorphism and emotional processing of social and nonsocial cues: an event-related potential (ERP) study |
| Journal | Journal of Physiological Anthropology. 2017. |
| Authors | Choi D, Minote N, Watanuki S. |
| Title | The myth of oral hygiene using synthetic mouthwash products |
| Journal | SpringerPlus. 2016. |
| Authors | Ghulam Zahara Jahangir, Durre Shahwar Ashraf, Idrees Ahmad Nasir, Madeha Sadiq, Sobia Shahzad, Farah Naz, Muhammad Iqbal and Afifa Saeed |
| Title | Case-control study of glucocorticoid receptor and corticotrophin-releasing hormone receptor gene variants and risk of perinatal depression |
| Journal | BMC Pregnancy and Childbirth. 2015. |
| Authors | Ene-Choo Tan, Tze-Ern Chua, Theresa M. Y. Lee, Hui-San Tan, Joe L. Y. Ting and Helen Y. Chen |
| Title | First Detection of Antibodies Against African Swine Fever Virus in Faeces Samples |
| Journal | Transboundary and Emerging Diseases. 2015. |
| Authors | E. Nieto-Pelegrın, B. Rivera-Arroyo and J. M. Sanchez-Vizcaıno |
| Title | 1000 Norms Project: protocol of a cross-sectional study cataloging human variation |
| Journal | Physiotherapy. 2015. |
| Authors | Marnee J. McKay, Jennifer N. Baldwin, Paulo Ferreira, Milena Simic, Natalie Vanicek, Claire E. Hiller, Elizabeth J. Nightingale, Niamh A. Moloney, Kate G. Quinlan, Fereshteh Pourkazemi, Amy D. Sman, Leslie L. Nicholson, Seyed J. Mousavi, Kristy Rose, Jacqueline Raymond, Martin G. Mackey, Angus Chard, Markus Hübscher, Caleb Wegener, Alycia Fong Yan, Kathryn M. Refshauge, Joshua Burns |
| Title | TAS2R38 single nucleotide polymorphisms are associated with PROP- but not thermal tasting: a pilot study. |
| Journal | Chemosensory Perception. 2013. |
| Authors | Bering A, Pickering G, Liang P. |
| Title | Brief communication: Evolution of a specific O allele (O1v(G542A) ) supports unique ancestry of Native Americans. |
| Journal | American Journal of Physical Anthropology. 2013. |
| Authors | Villanea FA, Bolnick DA, Monroe C, Worl R, Cambra R, Leventhal A, Kemp BM. |
| Title | Differences in the quantity of DNA found in the urine and saliva of smokers versus nonsmokers: implications for the timing of epigenetic events. |
| Journal | Epigenomics. 2012. |
| Authors | Simkin M, Abdalla M, El-Mogy M, Haj-Ahmad Y. |
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