Features and Benefits

This kit provides a rapid spin column procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on spin-column chromatography without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery.

The kit is also available in a 96-well format for high-throughput sequencing reaction clean-up.  Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Details

Supporting Data

Previous

Figure 1. Sequencing Chromatogram of pDC-CG DNA. A single extension product was divided into 3 equal aliquots and was cleaned using either Norgen's Sequencing Reaction Clean-Up Kit, or one of two competitor kits.  Sequencing data was then generated using Bigdye Terminator chemistry on the Applied Biosystems 3130xL DNA Sequencer. Panel A corresponds to the results when the sample was cleaned using Competitor 1, and this chromatogram indicates low extension product yields. Panel B corresponds to the results when the sample was cleaned using Competitor 2, and this product exhibited poor dye terminator removal that introduced several blobs to the sequence. Panel C corresponds to the sample cleaned with Norgen’s Sequencing Reaction Clean-Up Kit, and shows good yield and efficient removal of dye terminator and contaminants as indicated from the chromatogram signals.

1. 

Next

Click for expanded view

Documentation

Related Products

Sequencing Reaction Clean-Up 96-Well Kit

PCR Purification Kit

DNA Gel Extraction Kit