Sequencing Reaction Clean-Up Kit (Cat. 34500, 34400)

Purify sequencing extension products from dye terminators, primers and other contaminants
Also purify DNA from different enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations.
High recovery
Fast and efficient spin column format
Also available in 96 well format for high throughput

For the rapid purification of sequencing extension products from reaction mixes

Items	Cat. #	Size
Sequencing Reaction Clean-Up Kit	34500	50 preps
Sequencing Reaction Clean-Up 96-Well Kit	34400	2 x 96-well plates

Sequencing Reaction Clean-Up Kit

This kit provides a rapid spin column procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on spin-column chromatography without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery.

The kit is also available in a 96-well format for high-throughput sequencing reaction clean-up. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Kit Specifications - Spin Column
Column Binding Capacity	13 μg
Maximum Column Loading Volume	650 μL
Maximum Amount of Starting Material	25 μg
Time to Complete 10 Purifications	10 minutes
Minimum Elution Volume	30 μL

Kit Specifications - 96-well
Binding Capacity Per Well	15 μg
Maximum Loading Volume Per Well	500 μL
Average DNA Recovery	Up to 90%
Time to Complete 96 Purifications	20 minutes
Minimum Elution Volume	50 μL

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Protocols:

34500 - Protocol (50 prep)

34400 - Protocol (96-well)

Product Information Sheets:

Sequencing Reaction Clean-Up Kit - Product Information Sheet

Safety Data Sheets:

34400, 34500 - Safety Data Sheet

Items	Cat. #	Size
Sequencing Reaction Clean-Up Kit	34500	50 preps
Sequencing Reaction Clean-Up 96-Well Kit	34400	2 x 96-well plates

Sequencing Reaction Clean-Up Kit

This kit provides a rapid spin column procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on spin-column chromatography without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery.

The kit is also available in a 96-well format for high-throughput sequencing reaction clean-up. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Kit Specifications - Spin Column
Column Binding Capacity	13 μg
Maximum Column Loading Volume	650 μL
Maximum Amount of Starting Material	25 μg
Time to Complete 10 Purifications	10 minutes
Minimum Elution Volume	30 μL

Kit Specifications - 96-well
Binding Capacity Per Well	15 μg
Maximum Loading Volume Per Well	500 μL
Average DNA Recovery	Up to 90%
Time to Complete 96 Purifications	20 minutes
Minimum Elution Volume	50 μL

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.