For the rapid purification of sequencing extension products from reaction mixes
|Sequencing Reaction Clean-Up Kit||34500||50 preps|
|Sequencing Reaction Clean-Up 96-Well Kit||34400||2 x 96-well plates|
Sequencing Reaction Clean-Up Kit
This kit provides a rapid spin column procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on spin-column chromatography without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery.
The kit is also available in a 96-well format for high-throughput sequencing reaction clean-up. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
Kit Specifications - Spin Column
|Column Binding Capacity||13 μg|
|Maximum Column Loading Volume||650 μL|
|Maximum Amount of Starting Material||25 μg|
|Time to Complete 10 Purifications||10 minutes|
|Minimum Elution Volume||30 μL|
Kit Specifications - 96-well
|Binding Capacity Per Well||15 μg|
|Maximum Loading Volume Per Well||500 μL|
|Average DNA Recovery||Up to 90%|
|Time to Complete 96 Purifications||20 minutes|
|Minimum Elution Volume||50 μL|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
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