Sequencing Reaction Clean-Up Kit (Cat. 34500, 34400)

For the rapid purification of sequencing extension products from reaction mixes

  • Purify sequencing extension products from dye terminators, primers and other contaminants
  • Also purify DNA from different enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations.
  • High recovery
  • Fast and efficient spin column format
  • Also available in 96 well format for high throughput

 

ItemsCat. #Size
Sequencing Reaction Clean-Up Kit 34500 50 preps
Sequencing Reaction Clean-Up 96-Well Kit 34400 2 x 96-well plates

Sequencing Reaction Clean-Up Kit

This kit provides a rapid spin column procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on spin-column chromatography without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery.

The kit is also available in a 96-well format for high-throughput sequencing reaction clean-up.  Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Kit Specifications - Spin Column
Column Binding Capacity 13 μg
Maximum Column Loading Volume 650 μL
Maximum Amount of Starting Material 25 μg
Time to Complete 10 Purifications 10 minutes
Minimum Elution Volume 30 μL

 

Kit Specifications - 96-well
Binding Capacity Per Well 15 μg
Maximum Loading Volume Per Well 500 μL
Average DNA Recovery Up to 90%
Time to Complete 96 Purifications 20 minutes
Minimum Elution Volume 50 μL

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.