Sequencing Reaction Clean-Up Kit

Cat. 34500, 34400
  • Professional Tech Support with Senior R&D Scientist
  • Fast and Flexible Ordering Options (online, email, or phone)
  • Volume Discounts Available on Large Quantity Orders*

*Inquire by phone or email for more information.


Sequencing Reaction Clean-Up Kit

Cat. 34500, 34400

For the rapid purification of sequencing extension products from reaction mixes

  • Purify sequencing extension products from dye terminators, primers and other contaminants
  • Also purify DNA from different enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations.
  • High recovery
  • Fast and efficient spin column format
  • Also available in 96 well format for high throughput

 

For research use only and NOT intended for in vitro diagnostics.

Want a discount?

Create your account to receive 15% off on your first purchase!

Sequencing Reaction Clean-Up Kit
(Cat. 34500)
50 preps
Sequencing Reaction Clean-Up 96-Well Kit
(Cat. 34400)
2 x 96-well plates

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. 34500
Kit Specifications - Spin Column
Column Binding Capacity 13 μg
Maximum Column Loading Volume 650 μL
Maximum Amount of Starting Material 25 μg
Time to Complete 10 Purifications 10 minutes
Minimum Elution Volume 30 μL

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

You have selected: Cat. 34400
Kit Specifications - 96-well
Binding Capacity Per Well 15 μg
Maximum Loading Volume Per Well 500 μL
Average DNA Recovery Up to 90%
Time to Complete 96 Purifications 20 minutes
Minimum Elution Volume 50 μL

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Supporting Data

Click for expanded view

Sequencing Reaction Clean-Up Kit

This kit provides a rapid spin column procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on spin-column chromatography without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery.

The kit is also available in a 96-well format for high-throughput sequencing reaction clean-up.  Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 34500
Kit Specifications - Spin Column
Column Binding Capacity 13 μg
Maximum Column Loading Volume 650 μL
Maximum Amount of Starting Material 25 μg
Time to Complete 10 Purifications 10 minutes
Minimum Elution Volume 30 μL

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

You have selected: Cat. 34400
Kit Specifications - 96-well
Binding Capacity Per Well 15 μg
Maximum Loading Volume Per Well 500 μL
Average DNA Recovery Up to 90%
Time to Complete 96 Purifications 20 minutes
Minimum Elution Volume 50 μL

 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Related Products