Sequencing Reaction Clean-Up Kit For the rapid purification of sequencing extension products from reaction mixes For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Sequencing Reaction Clean-Up Kit For the rapid purification of sequencing extension products from reaction mixes Price USD$259.00 SKU 34400 Format Spin Column High Throughput Size 2 x 96-Well Plates Quantity Overview Details Documentation Citations Overview Purify sequencing extension products from dye terminators, primers and other contaminants Also purify DNA from different enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. High recovery Fast and efficient spin column format Also available in 96 well format for high throughput This kit provides a rapid spin column procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on spin-column chromatography without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery. The kit is also available in a 96-well format for high-throughput sequencing reaction clean-up. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation. Details Supporting Data Previous Figure 1. Sequencing Chromatogram of pDC-CG DNA. A single extension product was divided into 3 equal aliquots and was cleaned using either Norgen's Sequencing Reaction Clean-Up Kit, or one of two competitor kits. Sequencing data was then generated using Bigdye Terminator chemistry on the Applied Biosystems 3130xL DNA Sequencer. Panel A corresponds to the results when the sample was cleaned using Competitor 1, and this chromatogram indicates low extension product yields. Panel B corresponds to the results when the sample was cleaned using Competitor 2, and this product exhibited poor dye terminator removal that introduced several blobs to the sequence. Panel C corresponds to the sample cleaned with Norgen’s Sequencing Reaction Clean-Up Kit, and shows good yield and efficient removal of dye terminator and contaminants as indicated from the chromatogram signals. Next Click for expanded view Kit Specifications - 96-well Binding Capacity Per Well 15 μg Maximum Loading Volume Per Well 500 μL Average DNA Recovery Up to 90% Time to Complete 96 Purifications 20 minutes Minimum Elution Volume 50 μL Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. Component Cat. 34500 (50 preps) Cat. 34400 (2 x 96-well plate) Binding Buffer C 30 mL 3 x 30 mL Wash Solution A 12 mL 2 x 20 mL Elution Buffer B 8 mL 2 x 15 mL Spin Columns 50 - Collection Tubes 50 - Elution Tubes (1.7 mL) 50 - 96-Well Plate - 2 Adhesive Tape - 4 96-Well Collection Plate - 2 96-Well Elution Plate - 2 Product Insert 1 1 Documentation PROTOCOLS (34400) Sequencing Reaction Clean-Up 96-Well Kit (High Throughput) - Protocol (96-well) (34500) Sequencing Reaction Clean-Up Kit (Spin Column) - Protocol (50 prep) SAFETY DATA SHEETS (34400, 34500) Sequencing Reaction Clean-Up Kit - Safety Data Sheet Related Products Sequencing Reaction Clean-Up 96-Well Kit PCR Purification Kit DNA Gel Extraction Kit
Sequencing Reaction Clean-Up Kit For the rapid purification of sequencing extension products from reaction mixes For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Sequencing Reaction Clean-Up Kit For the rapid purification of sequencing extension products from reaction mixes Price USD$259.00 SKU 34400 Format Spin Column High Throughput Size 2 x 96-Well Plates Quantity