Rapid purification of total RNA -including microRNA - from small input amounts

  • Fast and easy processing using rapid micro spin-column format
  • Small elution volume of 8 µL ready for direct utilization in qRT-PCR reactions
  • Isolate total RNA including microRNA in a concentrated ready to use format
  • Isolate high quality total RNA from a variety of sources
  • RNA can be isolated from a single cell to 100,000 cells

ItemsCat. #Size
Single Cell RNA Purification Kit 51800 50 preps

Single Cell RNA Purification Kit

Very sensitive kit designed to work with as low as a single cell and up to 100,000 cells. The special design of the micro spin-column allows a small elution volume of as little as 8 µL.

Rapid 15 minute method for the isolation and purification of total RNA (including miRNA) from small input amounts of cultured animal cells, CTC, stem cells, immuno-sorted cells, and microdissected samples including laser-capture microdissection (LCM). The purified RNA is of the highest purity and integrity, and can be used in a number of qRT-PCR and digital PCR and other whole transcriptome applications.

Kit Specifications
Maximum Column Binding Capacity
10 μg
Maximum Column Loading Volume
650 μL
Minimum Elution Volume

8 μL

Size of RNA Purified
All sizes, including small RNA (<200 nt)
Acceptable Amount of Starting Material: Animal Cells
Laser-Captured Microdissection (LCM)
Single Cell to 2 x 105 cells
Up to 2 x 105 cells
Time to Complete 10 Purifications
20 minutes
Average Yields HeLa Cells (1 x 105 cells)

1.5 µg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 51800 (50 preps)
Buffer RL 40 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Single Cell RNA Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Journal Cell Reports. 2017.
Authors Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML.
Title Hox2 Genes Are Required for Tonotopic Map Precision and Sound Discrimination in the Mouse Auditory Brainstem
Journal Cell Reports. 2017.
Authors Karmakar K, Narita Y, Fadok J, Ducret S, Loche A, Kitazawa T, Genoud C, Di Meglio T, Thierry R, Bacelo J, Lüthi A, Rijli FM.
Title Dynamic Phosphorylation of the MEF2Cα1 Splice Variant Promotes Skeletal Muscle Regeneration and Hypertrophy
Journal Stem Cells. 2016.
Authors Baruffaldi F, Montarras D, Basile V, De Feo L, Badodi S, Ganassi M, Battini R, Nicoletti C, Imbriano C, Musarò A, Molinari S
Title Immunoprotective Primary Mesenchymal Stem Cells and Methods
Journal United States Patent Application 20160129110 . 2016.
Authors Garry RF, Branco LM, Bunnell BA, Wilson RB, Hopkins SE
Title The Response of microRNAs to Solar UVR in Skin-Resident Melanocytes Differs between Melanoma Patients and Healthy Persons
Journal PLOS ONE. 2016.
Authors Jingfeng Sha, Brian R. Gastman, Nathan Morris, Natasha A. Mesinkovska, Elma D. Baron, Kevin D. Cooper, Thomas McCormick, Joshua Arbesman, Marian L. Harter
Title Single-cell Migration Chip for Chemotaxis-based Microfluidic Selection of Heterogeneous Cell Populations
Journal Nature Scientific Reports. 2015.
Authors Yu-Chih Chen, Steven G. Allen, Patrick N. Ingram, Ronald Buckanovich, Sofia D. Merajver & Euisik Yoon
Title Defective microglial development in the hippocampus of Cx3cr1 deficient mice
Journal Frontiers in Cellular Neuroscience. 2015.
Authors Francesca Pagani, Rosa Paolicelli, Emanuele Murana, Barbara Cortese, Silvia Di_Angelantonio, Emanuele Zurolo, Eva Guiducci, Tiago A. Ferreira, Stefano Garofalo, Myriam Catalano, Giuseppina D_Alessandro, Alessandra Porzia, Giovanna Peruzzi, Fabrizio Mainiero, Cristina Limatola, Cornelius Gross and Davide Ragozzino