Single Cell RNA Purification Kit
Rapid purification of total RNA - including microRNA - from small input amounts
For research use only and NOT intended for in vitro diagnostics.
Single Cell RNA Purification Kit
Rapid purification of total RNA - including microRNA - from small input amounts
Overview
- Fast and easy processing using rapid micro spin column format
- Small elution volume of 8µL ready for direct utilization in qRT-PCR reactions
- Isolate total RNA including microRNA in a concentrated ready-to-use format
- Isolate high quality total RNA from a variety of sources
- RNA can be isolated from a single cell to 100,000 cells
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Very sensitive kit designed to work with as low as a single cell and up to 100,000 cells. The special design of the micro spin-column allows a small elution volume of as little as 8 µL.
Rapid 15 minute method for the isolation and purification of total RNA (including miRNA) from small input amounts of cultured animal cells, CTC, stem cells, immuno-sorted cells, and microdissected samples including laser-capture microdissection (LCM). The purified RNA is of the highest purity and integrity, and can be used in a number of qRT-PCR and digital PCR and other whole transcriptome applications.
Details
Supporting Data
Kit Specifications
|
|
Maximum Column Binding Capacity
|
10 μg
|
Maximum Column Loading Volume
|
650 μL
|
Minimum Elution Volume
|
8 μL |
Size of RNA Purified |
All sizes, including small RNA (<200 nt)
|
Maximum Amount of Starting Material: Animal Cells Laser-Captured Microdissection (LCM) |
Single Cell to 2 x 105 cells Up to 2 x 105 cells |
Time to Complete 10 Purifications
|
20 minutes
|
Average Yield HeLa Cells (1 x 105 cells) HeLa Cells (100 cells) HeLa Cells (1 cell) |
~ 1.5 µg ~ 1.5 ng ≥ 1pg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 51800 (50 preps) |
---|---|
Buffer RL | 40 mL |
Wash Solution A | 38 mL |
Elution Solution A | 6 mL |
Single Cell RNA Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |