Single Cell RNA Purification Kit Rapid purification of total RNA - including microRNA - from small input amounts For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Single Cell RNA Purification Kit Rapid purification of total RNA - including microRNA - from small input amounts Price USD$429.00 SKU 51800 Format Spin Column Size 50 Preps Quantity Request sample Overview Details Documentation Citations Overview Fast and easy processing using rapid micro spin column format Small elution volume of 8µL ready for direct utilization in qRT-PCR reactions Isolate total RNA including microRNA in a concentrated ready-to-use format Isolate high quality total RNA from a variety of sources RNA can be isolated from a single cell to 100,000 cells Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Very sensitive kit designed to work with as low as a single cell and up to 100,000 cells. The special design of the micro spin-column allows a small elution volume of as little as 8 µL. Rapid 15 minute method for the isolation and purification of total RNA (including miRNA) from small input amounts of cultured animal cells, CTC, stem cells, immuno-sorted cells, and microdissected samples including laser-capture microdissection (LCM). The purified RNA is of the highest purity and integrity, and can be used in a number of qRT-PCR and digital PCR and other whole transcriptome applications. Details Supporting Data Previous Figure 1. Better Efficiency of RNA Recovery at Single Cell Input. Norgen’s Single Cell RNA Purification Kit allows sensitive RNA extraction from as little as a single cell and outperforms competitor’s kits that claim similar sensitivity. Total RNA was extracted from a decreasing number of HeLa cells using Norgen’s Single Cell RNA Purification Kit and a competitor’s spin column purification kit. The extracted RNA was subjected to RT-qPCR to detect the human S15 and 5S rRNA transcript in the isolated RNA. RNA isolated by Norgen’s Single Cell RNA Purification Kit showed much higher relative expression of each RNA transcript tested at all cell input numbers down to single cell. Figure 2. High Quality RNA Isolated from Laser-Captured Microdissection (LCM). Norgen’s Single Cell RNA Purification Kit allows sensitive but high-quality RNA extraction from small inputs such as Laser-Captured Microdissection (LCM). Total RNA was isolated from 6 different LCM samples. RNA yield and quality (260/280 ratio) were assessed by NanoVue spectrophotometry. Norgen’s Single Cell RNA Purification Kit recovered good amounts of RNA with an excellent 260/280 ratio. Figure 3. Consistent and Linear Recovery of All Sizes of RNA down to Single Cell Input. Norgen’s Single Cell RNA Purification Kit allows sensitive RNA extraction from as little as a single cell with great linearity. Total RNA was extracted from a decreasing number of HeLa cells using Norgen’s Single Cell RNA Purification Kit. The extracted RNA was subjected to RT-qPCR to detect all sizes of RNA, including the human S15 (mRNA), 5S rRNA transcript (small RNA), and miR-21 (microRNA). RNA isolated by Norgen’s Single Cell RNA Purification Kit showed very high consistency (over 99%) of recovery of each RNA transcript tested at all cell input numbers down to a single cell. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity 10 μg Maximum Column Loading Volume 650 μL Minimum Elution Volume 8 μL Size of RNA Purified All sizes, including small RNA (<200 nt) Maximum Amount of Starting Material: Animal Cells Laser-Captured Microdissection (LCM) Single Cell to 2 x 105 cells Up to 2 x 105 cells Time to Complete 10 Purifications 20 minutes Average Yield HeLa Cells (1 x 105 cells) HeLa Cells (100 cells) HeLa Cells (1 cell) ~ 1.5 µg ~ 1.5 ng ≥ 1pg Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 51800 (50 preps) Buffer RL 40 mL Wash Solution A 38 mL Elution Solution A 6 mL Single Cell RNA Spin Columns 50 Collection Tubes 50 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (51800) Single Cell RNA Purification Kit - Protocol (50 prep) SAFETY DATA SHEETS (51800) Single Cell RNA Purification Kit - Safety Data Sheet FLYERS Flyer RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Powered by Bioz See more details on Bioz Related Products Animal Tissue RNA Purification Kit RNase-Free DNase I Kit Total RNA Purification Kit
Single Cell RNA Purification Kit Rapid purification of total RNA - including microRNA - from small input amounts For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Single Cell RNA Purification Kit Rapid purification of total RNA - including microRNA - from small input amounts Price USD$429.00 SKU 51800 Format Spin Column Size 50 Preps Quantity Request sample
