Single Cell RNA Purification Kit

Cat. 51800
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Single Cell RNA Purification Kit

Cat. 51800

Rapid purification of total RNA - including microRNA - from small input amounts

  • Fast and easy processing using rapid micro spin column format
  • Small elution volume of 8µL ready for direct utilization in qRT-PCR reactions
  • Isolate total RNA including microRNA in a concentrated ready-to-use format
  • Isolate high quality total RNA from a variety of sources
  • RNA can be isolated from a single cell to 100,000 cells
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Single Cell RNA Purification Kit
(Cat. 51800)
50 preps

Kit Size

  • Fast and easy processing using rapid micro spin column format
  • Small elution volume of 8µL ready for direct utilization in qRT-PCR reactions
  • Isolate total RNA including microRNA in a concentrated ready-to-use format
  • Isolate high quality total RNA from a variety of sources
  • RNA can be isolated from a single cell to 100,000 cells
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Single Cell RNA Purification Kit

Very sensitive kit designed to work with as low as a single cell and up to 100,000 cells. The special design of the micro spin-column allows a small elution volume of as little as 8 µL.

Rapid 15 minute method for the isolation and purification of total RNA (including miRNA) from small input amounts of cultured animal cells, CTC, stem cells, immuno-sorted cells, and microdissected samples including laser-capture microdissection (LCM). The purified RNA is of the highest purity and integrity, and can be used in a number of qRT-PCR and digital PCR and other whole transcriptome applications.

Kit Specifications

You have selected: Cat. 51800
Kit Specifications
Maximum Column Binding Capacity
10 μg
Maximum Column Loading Volume
650 μL
Minimum Elution Volume

8 μL

Size of RNA Purified
All sizes, including small RNA (<200 nt)
Maximum Amount of Starting Material:
Animal Cells
Laser-Captured Microdissection (LCM)
Single Cell to 2 x 105 cells
Up to 2 x 105 cells
Time to Complete 10 Purifications
20 minutes

Average Yield

HeLa Cells (1 x 105 cells)

HeLa Cells (100 cells)

HeLa Cells (1 cell)

 

~ 1.5 µg

~ 1.5 ng

≥ 1pg


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Supporting Data

Click for expanded view

Component Cat. 51800 (50 preps)
Buffer RL 40 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Single Cell RNA Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Selective isolation of mouse glial nuclei optimized for reliable downstream omics analyses
Journal bioRxiv. 2022.
Authors Miguel A. Pena-Ortiz, Sarfraz Shafiq, Megan E. Rowland and Nathalie G. Bérubé
Title Testicular Torsion: Preliminary Results of In Vitro Cell Stimulation Using Chorionic Gonadotropin
Journal Cells. 2022.
Authors Andrea Errico, Francesco Saverio Camoglio, Nicola Zampieri and Ilaria Dando
Title Selective isolation of mouse glial nuclei optimized for reliable downstream omics analyses
Journal Paediatrics Publications. 2022.
Authors Miguel A. Pena-Ortiz, Sarfraz Shafiq, Megan E. Rowland, Nathalie G. Bérubé
Title HLA-B∗46 associates with rapid HIV disease progression in Asian cohorts and prominent differences in NK cell phenotype
Journal Cell Host & Microbe. 2022.
Authors Shuying S.Li, Andrew Hickey, Shida Shangguan, Philip K. Ehrenberg, Aviva Geretz, Lauryn Butler, Gautam Kundu, et al.
Title Lung tumor MHCII immunity depends on in situ antigen presentation by fibroblasts
Journal J Exp Med. 2022.
Authors Dimitra Kerdidani, Emmanouil Aerakis, Kleio-Maria Verrou, Ilias Angelidis, Katerina Douka , Maria-Anna Maniou, Petros Stamoulis, Katerina Goudevenou, Alejandro Prados, Christos Tzaferis, Vasileios Ntafis, Ioannis Vamvakaris, Evangelos Kaniaris, et al.
Title Nr2f1a maintains atrial nkx2.5 expression to repress pacemaker identity within venous atrial cardiomyocytes
Journal bioRxiv. 2022.
Authors Kendall E. Martin, Padmapriyadarshini Ravisankar, Manu E. M. Beerens, Calum A. MacRae, Joshua S. Waxman
Title Size‑based isolation and detection of renal carcinoma cells from whole blood
Journal Molecular and Clinical Oncology. 2022.
Authors Roger De Alwis, Jennifer Hansson, David Lindgren, Sarah Schoch, Alexander Tejera, Bianca Scholtz, Peter Elfving, Christina Möller, Helén Nilsson, Martin Johansson, Håkan Axelson
Title Patient-derived micro-organospheres enable clinical precision oncology
Journal Cell Stem Cell. 2022.
Authors Shengli Ding, Carolyn Hsu, Zhaohui Wang, Naveen R. Natesh, Rosemary Millen, Marcos Negrete, Nicholas Giroux, Grecia O.Rivera, Anders Dohlman, Shree Bose, Tomer Rotstein, Kassandr Spiller, Athena Yeung, Zhiguo Sun, Chongming Jiang, Rui Xi, Benjamin Wilkin, Peggy M.Randon, Ian Williamson, Daniel A.Nelson, Daniel Delubac, Sehwa Oh, Gabrielle Rupprecht, James Isaacs, Jingquan Jia, Chao Chen, John Paul Shen, Scott Kopetz, Shannon McCall, Amber Smith, Nikolche Gjorevski, Antje-Christine Walz, Scott Antonia, Estelle Marrer-Berger, Hans Clevers, David Hsu, Xiling Shen
Title DNA-PKcs promotes sepsis-induced multiple organ failure by triggering mitochondrial dysfunction
Journal Journal of Advanced Research. 2022.
Authors Rongjun Zou, Jun Tao, Junxiong Qiu, Huimin Lu, Jianhua Wu, Hang Zhu, Ruibing Li, David Mui, Sam Toan, Xing Chang, Hao Zhou, Xiaoping Fan
Title Cell invasion in digital microfluidic microgel systems
Journal Science Advances. 2020.
Authors Bingyu B. Li, Erica Y. Scott, M. Dean Chamberlain, Bill T. V. Duong, Shuailong Zhang, Susan J. Done, Aaron R. Wheeler
Title Extracellular Vesicles in the Forebrain Display Reduced miR-346 and miR-331-3p in a Rat Model of Chronic Temporal Lobe Epilepsy
Journal Molecular Neurobiology. 2019.
Authors Daniel Leite Góes Gita, Ygor Daniel Ramos dos Santos, Raghavendra Upadhya, Maheedhar Kodali, Leelavathi N. Madhu & Ashok K. Shetty.
Title miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Journal Cell Reports. 2017.
Authors Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML.
Title Hox2 Genes Are Required for Tonotopic Map Precision and Sound Discrimination in the Mouse Auditory Brainstem
Journal Cell Reports. 2017.
Authors Karmakar K, Narita Y, Fadok J, Ducret S, Loche A, Kitazawa T, Genoud C, Di Meglio T, Thierry R, Bacelo J, Lüthi A, Rijli FM.
Title Dynamic Phosphorylation of the MEF2Cα1 Splice Variant Promotes Skeletal Muscle Regeneration and Hypertrophy
Journal Stem Cells. 2016.
Authors Baruffaldi F, Montarras D, Basile V, De Feo L, Badodi S, Ganassi M, Battini R, Nicoletti C, Imbriano C, Musarò A, Molinari S
Title Immunoprotective Primary Mesenchymal Stem Cells and Methods
Journal United States Patent Application 20160129110 . 2016.
Authors Garry RF, Branco LM, Bunnell BA, Wilson RB, Hopkins SE
Title The Response of microRNAs to Solar UVR in Skin-Resident Melanocytes Differs between Melanoma Patients and Healthy Persons
Journal PLOS ONE. 2016.
Authors Jingfeng Sha, Brian R. Gastman, Nathan Morris, Natasha A. Mesinkovska, Elma D. Baron, Kevin D. Cooper, Thomas McCormick, Joshua Arbesman, Marian L. Harter
Title Single-cell Migration Chip for Chemotaxis-based Microfluidic Selection of Heterogeneous Cell Populations
Journal Nature Scientific Reports. 2015.
Authors Yu-Chih Chen, Steven G. Allen, Patrick N. Ingram, Ronald Buckanovich, Sofia D. Merajver & Euisik Yoon
Title Defective microglial development in the hippocampus of Cx3cr1 deficient mice
Journal Frontiers in Cellular Neuroscience. 2015.
Authors Francesca Pagani, Rosa Paolicelli, Emanuele Murana, Barbara Cortese, Silvia Di_Angelantonio, Emanuele Zurolo, Eva Guiducci, Tiago A. Ferreira, Stefano Garofalo, Myriam Catalano, Giuseppina D_Alessandro, Alessandra Porzia, Giovanna Peruzzi, Fabrizio Mainiero, Cristina Limatola, Cornelius Gross and Davide Ragozzino

Single Cell RNA Purification Kit

Very sensitive kit designed to work with as low as a single cell and up to 100,000 cells. The special design of the micro spin-column allows a small elution volume of as little as 8 µL.

Rapid 15 minute method for the isolation and purification of total RNA (including miRNA) from small input amounts of cultured animal cells, CTC, stem cells, immuno-sorted cells, and microdissected samples including laser-capture microdissection (LCM). The purified RNA is of the highest purity and integrity, and can be used in a number of qRT-PCR and digital PCR and other whole transcriptome applications.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 51800
Kit Specifications
Maximum Column Binding Capacity
10 μg
Maximum Column Loading Volume
650 μL
Minimum Elution Volume

8 μL

Size of RNA Purified
All sizes, including small RNA (<200 nt)
Maximum Amount of Starting Material:
Animal Cells
Laser-Captured Microdissection (LCM)
Single Cell to 2 x 105 cells
Up to 2 x 105 cells
Time to Complete 10 Purifications
20 minutes

Average Yield

HeLa Cells (1 x 105 cells)

HeLa Cells (100 cells)

HeLa Cells (1 cell)

 

~ 1.5 µg

~ 1.5 ng

≥ 1pg


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component Cat. 51800 (50 preps)
Buffer RL 40 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Single Cell RNA Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Selective isolation of mouse glial nuclei optimized for reliable downstream omics analyses
Journal bioRxiv. 2022.
Authors Miguel A. Pena-Ortiz, Sarfraz Shafiq, Megan E. Rowland and Nathalie G. Bérubé
Title Testicular Torsion: Preliminary Results of In Vitro Cell Stimulation Using Chorionic Gonadotropin
Journal Cells. 2022.
Authors Andrea Errico, Francesco Saverio Camoglio, Nicola Zampieri and Ilaria Dando
Title Selective isolation of mouse glial nuclei optimized for reliable downstream omics analyses
Journal Paediatrics Publications. 2022.
Authors Miguel A. Pena-Ortiz, Sarfraz Shafiq, Megan E. Rowland, Nathalie G. Bérubé
Title HLA-B∗46 associates with rapid HIV disease progression in Asian cohorts and prominent differences in NK cell phenotype
Journal Cell Host & Microbe. 2022.
Authors Shuying S.Li, Andrew Hickey, Shida Shangguan, Philip K. Ehrenberg, Aviva Geretz, Lauryn Butler, Gautam Kundu, et al.
Title Lung tumor MHCII immunity depends on in situ antigen presentation by fibroblasts
Journal J Exp Med. 2022.
Authors Dimitra Kerdidani, Emmanouil Aerakis, Kleio-Maria Verrou, Ilias Angelidis, Katerina Douka , Maria-Anna Maniou, Petros Stamoulis, Katerina Goudevenou, Alejandro Prados, Christos Tzaferis, Vasileios Ntafis, Ioannis Vamvakaris, Evangelos Kaniaris, et al.
Title Nr2f1a maintains atrial nkx2.5 expression to repress pacemaker identity within venous atrial cardiomyocytes
Journal bioRxiv. 2022.
Authors Kendall E. Martin, Padmapriyadarshini Ravisankar, Manu E. M. Beerens, Calum A. MacRae, Joshua S. Waxman
Title Size‑based isolation and detection of renal carcinoma cells from whole blood
Journal Molecular and Clinical Oncology. 2022.
Authors Roger De Alwis, Jennifer Hansson, David Lindgren, Sarah Schoch, Alexander Tejera, Bianca Scholtz, Peter Elfving, Christina Möller, Helén Nilsson, Martin Johansson, Håkan Axelson
Title Patient-derived micro-organospheres enable clinical precision oncology
Journal Cell Stem Cell. 2022.
Authors Shengli Ding, Carolyn Hsu, Zhaohui Wang, Naveen R. Natesh, Rosemary Millen, Marcos Negrete, Nicholas Giroux, Grecia O.Rivera, Anders Dohlman, Shree Bose, Tomer Rotstein, Kassandr Spiller, Athena Yeung, Zhiguo Sun, Chongming Jiang, Rui Xi, Benjamin Wilkin, Peggy M.Randon, Ian Williamson, Daniel A.Nelson, Daniel Delubac, Sehwa Oh, Gabrielle Rupprecht, James Isaacs, Jingquan Jia, Chao Chen, John Paul Shen, Scott Kopetz, Shannon McCall, Amber Smith, Nikolche Gjorevski, Antje-Christine Walz, Scott Antonia, Estelle Marrer-Berger, Hans Clevers, David Hsu, Xiling Shen
Title DNA-PKcs promotes sepsis-induced multiple organ failure by triggering mitochondrial dysfunction
Journal Journal of Advanced Research. 2022.
Authors Rongjun Zou, Jun Tao, Junxiong Qiu, Huimin Lu, Jianhua Wu, Hang Zhu, Ruibing Li, David Mui, Sam Toan, Xing Chang, Hao Zhou, Xiaoping Fan
Title Cell invasion in digital microfluidic microgel systems
Journal Science Advances. 2020.
Authors Bingyu B. Li, Erica Y. Scott, M. Dean Chamberlain, Bill T. V. Duong, Shuailong Zhang, Susan J. Done, Aaron R. Wheeler
Title Extracellular Vesicles in the Forebrain Display Reduced miR-346 and miR-331-3p in a Rat Model of Chronic Temporal Lobe Epilepsy
Journal Molecular Neurobiology. 2019.
Authors Daniel Leite Góes Gita, Ygor Daniel Ramos dos Santos, Raghavendra Upadhya, Maheedhar Kodali, Leelavathi N. Madhu & Ashok K. Shetty.
Title miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Journal Cell Reports. 2017.
Authors Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML.
Title Hox2 Genes Are Required for Tonotopic Map Precision and Sound Discrimination in the Mouse Auditory Brainstem
Journal Cell Reports. 2017.
Authors Karmakar K, Narita Y, Fadok J, Ducret S, Loche A, Kitazawa T, Genoud C, Di Meglio T, Thierry R, Bacelo J, Lüthi A, Rijli FM.
Title Dynamic Phosphorylation of the MEF2Cα1 Splice Variant Promotes Skeletal Muscle Regeneration and Hypertrophy
Journal Stem Cells. 2016.
Authors Baruffaldi F, Montarras D, Basile V, De Feo L, Badodi S, Ganassi M, Battini R, Nicoletti C, Imbriano C, Musarò A, Molinari S
Title Immunoprotective Primary Mesenchymal Stem Cells and Methods
Journal United States Patent Application 20160129110 . 2016.
Authors Garry RF, Branco LM, Bunnell BA, Wilson RB, Hopkins SE
Title The Response of microRNAs to Solar UVR in Skin-Resident Melanocytes Differs between Melanoma Patients and Healthy Persons
Journal PLOS ONE. 2016.
Authors Jingfeng Sha, Brian R. Gastman, Nathan Morris, Natasha A. Mesinkovska, Elma D. Baron, Kevin D. Cooper, Thomas McCormick, Joshua Arbesman, Marian L. Harter
Title Single-cell Migration Chip for Chemotaxis-based Microfluidic Selection of Heterogeneous Cell Populations
Journal Nature Scientific Reports. 2015.
Authors Yu-Chih Chen, Steven G. Allen, Patrick N. Ingram, Ronald Buckanovich, Sofia D. Merajver & Euisik Yoon
Title Defective microglial development in the hippocampus of Cx3cr1 deficient mice
Journal Frontiers in Cellular Neuroscience. 2015.
Authors Francesca Pagani, Rosa Paolicelli, Emanuele Murana, Barbara Cortese, Silvia Di_Angelantonio, Emanuele Zurolo, Eva Guiducci, Tiago A. Ferreira, Stefano Garofalo, Myriam Catalano, Giuseppina D_Alessandro, Alessandra Porzia, Giovanna Peruzzi, Fabrizio Mainiero, Cristina Limatola, Cornelius Gross and Davide Ragozzino

 


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