For research use only and NOT intended for in vitro diagnostics.
For the rapid and simple purification of bacterial and host DNA from stool and fecal samples.
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Stool DNA Isolation Kit
This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and other inhibitors using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Kit Specifications (Cat No. 27600)
|
|
Maximum Stool Input
|
200 mg (fresh/frozen stool) or 400 μL (preserved stool)
|
Type of Stool Processed
|
Frozen, fresh or preserved stool
|
Format | Spin Column |
Maximum Column Binding Capacity
|
50 μg
|
Maximum Column Loading Volume |
650 μL
|
Elution Volume | 50 μL |
Time to Complete 10 Purifications |
30 minutes
|
Applications |
PCR, qPCR, Southern Blot Analysis, |
Kit Specifications (Cat No. 65600)
|
|
Binding Capacity Per Well
|
50 μg
|
Maximum Loading Volume Per Well |
500 μL
|
Size of DNA Purified | All sizes |
Maximum Amount of Starting Material: |
200 μg 400 μL |
Time to Complete 96 Purifications |
60 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Title | Multilocus Sequence Typing helps understand the genetic diversity of Cryptosporidium hominis and Cryptosporidium parvum isolated from Colombian patients |
Journal | Plos One. 2022 |
Authors | Johanna Uran-Velasquez, Juan F. Alzate, Ana E. Farfan-Garcia, Oscar G. Gomez-Duarte, Larry L. Martinez-Rosado, Diego D. Dominguez-Hernandez, Winston Rojas, Ana Luz Galvan-Diaz, Gisela M. Garcia-Montoya |
Title | Development of a rational framework for the therapeutic efficacy of fecal microbiota transplantation for calf diarrhea treatment |
Journal | Microbiome. 2022 |
Authors | Islam J, Tanimizu M, Shimizu Y, Goto Y, Ohtani N, Sugiyama K, Tatezaki E, Sato M, Makino E, Shimada T, Ueda C, Matsuo A, Suyama Y, Sakai Y, Furukawa M, Usami K, Yoneyama H, Aso H, Tanaka H, Nochi T |
Title | Molecular Epidemiology of Giardia, Blastocystis and Cryptosporidium among Indigenous Children from the Colombian Amazon Basin |
Journal | Frontiers in Microbology. 2017 |
Authors | Sánchez A, Munoz M, Gómez N, Tabares J, Segura L, Salazar Á, Restrepo C, Ruíz M, Reyes P, Qian Y, Xiao L, López MC, Ramírez JD. |
Title | A Rabbit Model of Acanthamoeba Keratitis: Use of Infected Soft Contact Lenses After Corneal Epithelium Debridement With a Diamond Burr |
Journal | Investigative Ophthalmology & Visual Science. 2017 |
Authors | Ortillés Á, Goñi P, Rubio E, Sierra M, Gámez E, Fernández MT, Benito M, Cristóbal JÁ, Calvo B. |
Title | Intestinal microbiota as a tetrahydrobiopterin exogenous source in hph-1 mice |
Journal | Scientific Reports. 2017. |
Authors | Belik J, Shifrin Y, Arning E, Bottiglieri T, Pan J, Daigneault MC, Allen-Vercoe E. |
Title | Method Optimization for Fecal Sample Collection and Fecal DNA Extraction |
Journal | Biopreservation Biobanking. 2015. |
Authors | Mathay C, Hamot G, Henry E, Georges L, Bellora C, Lebrun L, de Witt B, Ammerlaan W, Buschart A, Wilmes P, Betsou F. |
Title | Exceptional maternal lineage diversity in brown bears (Ursus arctos) from Turkey |
Journal | Zoological Journal of the Linnean Society. 2016. |
Authors | F. Gözde Çilingir, Çiğdem Akın Pekşen, Hüseyin Ambarlı, Peter Beerli and C. Can Bilgin |
Title | Changes on fecal microbiota in rats exposed to permethrin during postnatal development |
Journal | Environmental Science and Pollution Research International. 2016. |
Authors | Nasuti C, Coman MM, Olek RA, Fiorini D, Verdenelli MC, Cecchini C, Silvi S, Fedeli D, Gabbianelli R |
Title | An Independent Evaluation of the CryoXtract Instruments' CXT350 Frozen Sample Aliquotter Using Tissue and Fecal Biospecimens |
Journal | Biopreservation and Biobanking. 2016. |
Authors | Mathieson W, Sanchez I, Mommaerts K, Frasquilho S, Betsou F |
Title | Cryptosporidiosis outbreak in a child day-care center caused by an unusual Cryptosporidium hominis subtype |
Journal | Enfermedades Infecciosas y Microbiología Clínica. 2015. |
Authors | Pilar Goñi, Diego Almagro-Nievas, Joanna Cieloszyk, Silvia Lóbez, José María Navarro-Marí, José Gutiérrez-Fernández |
Title | Detection of Campylobacter in human faecal samples in Fiji |
Journal | Western Pacific Surveillance and Response Journal. 2014. |
Authors | Aruna Devi, Jenny Wilkinson, Timothy Mahony and Thiru Vanniasinkam |
Title | How to make DNA count: DNA-based diagnostic tools in veterinary parasitology. |
Journal | Veterinary Parasitology. 2011. |
Authors | Hunt PW, Lello J. |
Want a discount?
Create your account to receive 15% off on your first purchase!
Stool DNA Isolation Kit
This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and other inhibitors using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Kit Specifications (Cat No. 27600)
|
|
Maximum Stool Input
|
200 mg (fresh/frozen stool) or 400 μL (preserved stool)
|
Type of Stool Processed
|
Frozen, fresh or preserved stool
|
Format | Spin Column |
Maximum Column Binding Capacity
|
50 μg
|
Maximum Column Loading Volume |
650 μL
|
Elution Volume | 50 μL |
Time to Complete 10 Purifications |
30 minutes
|
Applications |
PCR, qPCR, Southern Blot Analysis, |
Kit Specifications (Cat No. 65600)
|
|
Binding Capacity Per Well
|
50 μg
|
Maximum Loading Volume Per Well |
500 μL
|
Size of DNA Purified | All sizes |
Maximum Amount of Starting Material: |
200 μg 400 μL |
Time to Complete 96 Purifications |
60 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Title | Multilocus Sequence Typing helps understand the genetic diversity of Cryptosporidium hominis and Cryptosporidium parvum isolated from Colombian patients |
Journal | Plos One. 2022 |
Authors | Johanna Uran-Velasquez, Juan F. Alzate, Ana E. Farfan-Garcia, Oscar G. Gomez-Duarte, Larry L. Martinez-Rosado, Diego D. Dominguez-Hernandez, Winston Rojas, Ana Luz Galvan-Diaz, Gisela M. Garcia-Montoya |
Title | Development of a rational framework for the therapeutic efficacy of fecal microbiota transplantation for calf diarrhea treatment |
Journal | Microbiome. 2022 |
Authors | Islam J, Tanimizu M, Shimizu Y, Goto Y, Ohtani N, Sugiyama K, Tatezaki E, Sato M, Makino E, Shimada T, Ueda C, Matsuo A, Suyama Y, Sakai Y, Furukawa M, Usami K, Yoneyama H, Aso H, Tanaka H, Nochi T |
Title | Molecular Epidemiology of Giardia, Blastocystis and Cryptosporidium among Indigenous Children from the Colombian Amazon Basin |
Journal | Frontiers in Microbology. 2017 |
Authors | Sánchez A, Munoz M, Gómez N, Tabares J, Segura L, Salazar Á, Restrepo C, Ruíz M, Reyes P, Qian Y, Xiao L, López MC, Ramírez JD. |
Title | A Rabbit Model of Acanthamoeba Keratitis: Use of Infected Soft Contact Lenses After Corneal Epithelium Debridement With a Diamond Burr |
Journal | Investigative Ophthalmology & Visual Science. 2017 |
Authors | Ortillés Á, Goñi P, Rubio E, Sierra M, Gámez E, Fernández MT, Benito M, Cristóbal JÁ, Calvo B. |
Title | Intestinal microbiota as a tetrahydrobiopterin exogenous source in hph-1 mice |
Journal | Scientific Reports. 2017. |
Authors | Belik J, Shifrin Y, Arning E, Bottiglieri T, Pan J, Daigneault MC, Allen-Vercoe E. |
Title | Method Optimization for Fecal Sample Collection and Fecal DNA Extraction |
Journal | Biopreservation Biobanking. 2015. |
Authors | Mathay C, Hamot G, Henry E, Georges L, Bellora C, Lebrun L, de Witt B, Ammerlaan W, Buschart A, Wilmes P, Betsou F. |
Title | Exceptional maternal lineage diversity in brown bears (Ursus arctos) from Turkey |
Journal | Zoological Journal of the Linnean Society. 2016. |
Authors | F. Gözde Çilingir, Çiğdem Akın Pekşen, Hüseyin Ambarlı, Peter Beerli and C. Can Bilgin |
Title | Changes on fecal microbiota in rats exposed to permethrin during postnatal development |
Journal | Environmental Science and Pollution Research International. 2016. |
Authors | Nasuti C, Coman MM, Olek RA, Fiorini D, Verdenelli MC, Cecchini C, Silvi S, Fedeli D, Gabbianelli R |
Title | An Independent Evaluation of the CryoXtract Instruments' CXT350 Frozen Sample Aliquotter Using Tissue and Fecal Biospecimens |
Journal | Biopreservation and Biobanking. 2016. |
Authors | Mathieson W, Sanchez I, Mommaerts K, Frasquilho S, Betsou F |
Title | Cryptosporidiosis outbreak in a child day-care center caused by an unusual Cryptosporidium hominis subtype |
Journal | Enfermedades Infecciosas y Microbiología Clínica. 2015. |
Authors | Pilar Goñi, Diego Almagro-Nievas, Joanna Cieloszyk, Silvia Lóbez, José María Navarro-Marí, José Gutiérrez-Fernández |
Title | Detection of Campylobacter in human faecal samples in Fiji |
Journal | Western Pacific Surveillance and Response Journal. 2014. |
Authors | Aruna Devi, Jenny Wilkinson, Timothy Mahony and Thiru Vanniasinkam |
Title | How to make DNA count: DNA-based diagnostic tools in veterinary parasitology. |
Journal | Veterinary Parasitology. 2011. |
Authors | Hunt PW, Lello J. |