For the rapid and simple purification of bacterial and host DNA from stool and fecal samples

  • Simultaneous isolation of both host DNA and microbial DNA (universal protocol)
  • Eliminates PCR inhibitors including humic acidsFully compatible with Norgen's Stool Nucleic Acid Collection and Transport Tubes
  • High quality DNA for sensitive downstream applications including PCR, qPCR, Sequencing and microarra
  • ​​​​​​​96-Well format is also available

ItemsCat. #Size
Stool DNA Isolation Kit 27600 50 preps
Stool DNA Isolation 96-Well Kit 65600 2 x 96-well plates

Stool DNA Isolation Kit

This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and other inhibitors using Bead Tubes and a combination of chemical and physical homogenization and lysis, without the need of any phenol-chloroform extractions. A simple and rapid spin column procedure is then used to further purify the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, Sequencing and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.

Kit Specifications (Cat No. 27600)
Maximum Stool Input
200 mg
Type of Stool Processed
Frozen, fresh or preserved stool
Format Spin Column
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Elution Volume 50 μL
Time to Complete 10 Purifications
30 minutes
Applications

PCR, qPCR, Southern Blot Analysis,
Sequencing, Microarray Analysis.

Kit Specifications (Cat No. 65600)
Binding Capacity Per Well
50 μg
Maximum Loading Volume Per Well
500 μL
Size of DNA Purified All sizes

Maximum Amount of Starting Material:
Fresh or frozen stool
Preserved stool*

200 μg
400 μL
Time to Complete 96 Purifications
60 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 27600 (50 preps) Cat. 65600 (2 x 96-Well)
Lysis Buffer L 60 mL 2 x 105 mL
Lysis Additive A 6 mL 25 mL
Binding Buffer I 7 mL 25 mL
Binding Buffer C 30 mL 110 mL
Wash Solution A 18 mL 2 x 38 mL mL
Elution Buffer B 8 mL 30 mL
Bead Tube 50 200
Mini Spin Columns 50 N/A
Collection Tubes 50 N/A
Elution Tubes (1.7 mL) 50 N/A
96-Well Plate N/A 2
96-Well Collection Plate N/A 2
96-Well Elution Plate N/A 2
Adhesive Tape N/A 4
Product Insert 1 1

Title Molecular Epidemiology of Giardia, Blastocystis and Cryptosporidium among Indigenous Children from the Colombian Amazon Basin
Journal Frontiers in Microbology. 2017
Authors Sánchez A, Munoz M, Gómez N, Tabares J, Segura L, Salazar Á, Restrepo C, Ruíz M, Reyes P, Qian Y, Xiao L, López MC, Ramírez JD.
Title A Rabbit Model of Acanthamoeba Keratitis: Use of Infected Soft Contact Lenses After Corneal Epithelium Debridement With a Diamond Burr
Journal Investigative Ophthalmology & Visual Science. 2017
Authors Ortillés Á, Goñi P, Rubio E, Sierra M, Gámez E, Fernández MT, Benito M, Cristóbal JÁ, Calvo B.
Title Intestinal microbiota as a tetrahydrobiopterin exogenous source in hph-1 mice
Journal Scientific Reports. 2017.
Authors Belik J, Shifrin Y, Arning E, Bottiglieri T, Pan J, Daigneault MC, Allen-Vercoe E.
Title Method Optimization for Fecal Sample Collection and Fecal DNA Extraction
Journal Biopreservation Biobanking. 2015.
Authors Mathay C, Hamot G, Henry E, Georges L, Bellora C, Lebrun L, de Witt B, Ammerlaan W, Buschart A, Wilmes P, Betsou F.
Title Exceptional maternal lineage diversity in brown bears (Ursus arctos) from Turkey
Journal Zoological Journal of the Linnean Society. 2016.
Authors F. Gözde Çilingir, Çiğdem Akın Pekşen, Hüseyin Ambarlı, Peter Beerli and C. Can Bilgin
Title Changes on fecal microbiota in rats exposed to permethrin during postnatal development
Journal Environmental Science and Pollution Research International. 2016.
Authors Nasuti C, Coman MM, Olek RA, Fiorini D, Verdenelli MC, Cecchini C, Silvi S, Fedeli D, Gabbianelli R
Title An Independent Evaluation of the CryoXtract Instruments' CXT350 Frozen Sample Aliquotter Using Tissue and Fecal Biospecimens
Journal Biopreservation and Biobanking. 2016.
Authors Mathieson W, Sanchez I, Mommaerts K, Frasquilho S, Betsou F
Title Cryptosporidiosis outbreak in a child day-care center caused by an unusual Cryptosporidium hominis subtype
Journal Enfermedades Infecciosas y Microbiología Clínica. 2015.
Authors Pilar Goñi, Diego Almagro-Nievas, Joanna Cieloszyk, Silvia Lóbez, José María Navarro-Marí, José Gutiérrez-Fernández
Title Detection of Campylobacter in human faecal samples in Fiji
Journal Western Pacific Surveillance and Response Journal. 2014.
Authors Aruna Devi, Jenny Wilkinson, Timothy Mahony and Thiru Vanniasinkam
Title How to make DNA count: DNA-based diagnostic tools in veterinary parasitology.
Journal Veterinary Parasitology. 2011.
Authors Hunt PW, Lello J.