Stool DNA Isolation Kit Dx (Cat. Dx27600)
Stool DNA Isolation Kit Dx
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Stool DNA Isolation Kit Dx
For the rapid and simple purification of bacterial and host DNA from stool and fecal samples for in vitro diagnostic use
- CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
- Ideal for use in in vitro diagnostic workflows
-
Simultaneous isolation of both host DNA and microbial DNA (universal protocol)
-
Eliminates PCR inhibitors including humic acids
-
Fully compatible with Norgen's Stool Nucleic Acid Collection and Transport Tubes
-
High quality DNA for sensitive downstream applications including PCR, qPCR, sequencing and microarray
​
Note: This product is not available for sale in the United States
Research versions available here
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(Cat. Dx27600) 50 preps
Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
Product Size
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Stool Input
|
200 mg (fresh/frozen stool) or 400 ÎĽL (preserved stool)
|
|
Type of Stool Processed
|
Frozen, fresh or preserved stool
|
| Format | Spin Column |
|
Maximum Column Binding Capacity
|
50 ÎĽg
|
| Maximum Column Loading Volume |
650 ÎĽL
|
| Elution Volume | 50 ÎĽL |
| Time to Complete 10 Purifications |
30 minutes
|
| Applications |
PCR, qPCR, Southern Blot Analysis, |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Supporting Data
| Component | Cat. Dx27600 (50 preps) |
|---|---|
| Lysis Solution | 60 mL |
| Lysis Additive | 6 mL |
| Binding Solution | 7 mL |
| Wash Solution I | 30 mL |
| Wash Solution II | 22 mL |
| Elution Buffer | 3 mL |
| Bead Tube | 50 |
| Mini Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | Changes on fecal microbiota in rats exposed to permethrin during postnatal development |
| Journal | Environmental Science and Pollution Research International. 2016. |
| Authors | Nasuti C, Coman MM, Olek RA, Fiorini D, Verdenelli MC, Cecchini C, Silvi S, Fedeli D, Gabbianelli R |
| Title | An Independent Evaluation of the CryoXtract Instruments' CXT350 Frozen Sample Aliquotter Using Tissue and Fecal Biospecimens |
| Journal | Biopreservation and Biobanking. 2016. |
| Authors | Mathieson W, Sanchez I, Mommaerts K, Frasquilho S, Betsou F |
| Title | Cryptosporidiosis outbreak in a child day-care center caused by an unusual Cryptosporidium hominis subtype |
| Journal | Enfermedades Infecciosas y MicrobiologĂa ClĂnica. 2015. |
| Authors | Pilar Goñi, Diego Almagro-Nievas, Joanna Cieloszyk, Silvia LĂłbez, JosĂ© MarĂa Navarro-MarĂ, JosĂ© GutiĂ©rrez-Fernández |
| Title | Detection of Campylobacter in human faecal samples in Fiji |
| Journal | Western Pacific Surveillance and Response Journal. 2014. |
| Authors | Aruna Devi, Jenny Wilkinson, Timothy Mahony and Thiru Vanniasinkam |
| Title | How to make DNA count: DNA-based diagnostic tools in veterinary parasitology. |
| Journal | Veterinary Parasitology. 2011. |
| Authors | Hunt PW, Lello J. |
Stool DNA Isolation Kit Dx
This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Devices Dx (Cat. Dx45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and other inhibitors using Bead Tubes and a combination of chemical and physical homogenization and lysis, without the need of any phenol-chloroform extractions. A simple and rapid spin column procedure is then used to further purify the DNA with high yields and molecular weights of up to 50 kb plus.  The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
NOTE: This product is not available for sale in the United States.
Supporting Data
Kit Specifications
|
Kit Specifications
|
|
|
Maximum Stool Input
|
200 mg (fresh/frozen stool) or 400 ÎĽL (preserved stool)
|
|
Type of Stool Processed
|
Frozen, fresh or preserved stool
|
| Format | Spin Column |
|
Maximum Column Binding Capacity
|
50 ÎĽg
|
| Maximum Column Loading Volume |
650 ÎĽL
|
| Elution Volume | 50 ÎĽL |
| Time to Complete 10 Purifications |
30 minutes
|
| Applications |
PCR, qPCR, Southern Blot Analysis, |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. Dx27600 (50 preps) |
|---|---|
| Lysis Solution | 60 mL |
| Lysis Additive | 6 mL |
| Binding Solution | 7 mL |
| Wash Solution I | 30 mL |
| Wash Solution II | 22 mL |
| Elution Buffer | 3 mL |
| Bead Tube | 50 |
| Mini Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
| Title | Changes on fecal microbiota in rats exposed to permethrin during postnatal development |
| Journal | Environmental Science and Pollution Research International. 2016. |
| Authors | Nasuti C, Coman MM, Olek RA, Fiorini D, Verdenelli MC, Cecchini C, Silvi S, Fedeli D, Gabbianelli R |
| Title | An Independent Evaluation of the CryoXtract Instruments' CXT350 Frozen Sample Aliquotter Using Tissue and Fecal Biospecimens |
| Journal | Biopreservation and Biobanking. 2016. |
| Authors | Mathieson W, Sanchez I, Mommaerts K, Frasquilho S, Betsou F |
| Title | Cryptosporidiosis outbreak in a child day-care center caused by an unusual Cryptosporidium hominis subtype |
| Journal | Enfermedades Infecciosas y MicrobiologĂa ClĂnica. 2015. |
| Authors | Pilar Goñi, Diego Almagro-Nievas, Joanna Cieloszyk, Silvia LĂłbez, JosĂ© MarĂa Navarro-MarĂ, JosĂ© GutiĂ©rrez-Fernández |
| Title | Detection of Campylobacter in human faecal samples in Fiji |
| Journal | Western Pacific Surveillance and Response Journal. 2014. |
| Authors | Aruna Devi, Jenny Wilkinson, Timothy Mahony and Thiru Vanniasinkam |
| Title | How to make DNA count: DNA-based diagnostic tools in veterinary parasitology. |
| Journal | Veterinary Parasitology. 2011. |
| Authors | Hunt PW, Lello J. |
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