Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.
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Stool DNA Isolation Kit Dx
CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Ideal for use in in vitro diagnostic workflows
Simultaneous isolation of both host DNA and microbial DNA (universal protocol)
Eliminates PCR inhibitors including humic acids
Fully compatible with Norgen's Stool Nucleic Acid Collection and Transport Tubes
High quality DNA for sensitive downstream applications including PCR, qPCR, sequencing and microarray
This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Devices Dx (Cat. Dx45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and other inhibitors using Bead Tubes and a combination of chemical and physical homogenization and lysis, without the need of any phenol-chloroform extractions. A simple and rapid spin column procedure is then used to further purify the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
NOTE: This product is not available for sale in the United States.
Figure 1. Higher Yields of DNA than Competitor Z. Stool DNA was isolated from 200 mg of fresh stool samples using Norgens Stool DNA Isolation Kit Dx and Competitor Z's Kit. For evaluation, 10 µL of DNA from the elution was run on 1X TAE 1.2% agarose gel. Norgen's kit isolated much higher yields of DNA. Marker= Norgens HighRanger DNA Ladder.
Figure 2. Higher Quality DNA than Competitor Z. Stool DNA was isolated from 200 mg of fresh stool samples using Norgen's Stool DNA Isolation Kit Dx and Competitor Z's Kit. DNA quality was then was measured by Nanodrop. Norgen's Stool DNA Isolation Kit Dx isolated DNA of the highest quality.
Figure 3. High Quality DNA confirmed by Real-time PCR. Stool DNA was isolated from 200 mg of fresh stool samples using Norgen's Stool DNA Isolation Kit Dx and Competitor Z's Kit. DNA quality was confirmed by real-time PCR using 4 µL of stool DNA (total PCR reaction volume was 20 µL) to detect 16S rRNA from different stool samples. The earlier Ct value with Norgen's DNA samples (blue lines) compared to Competitor Z's samples (red lines) indicated a higher quality of stool DNA for downstream applications.
Figure 4. Taxonomic Classifications of 10 Fecal Microbiomes. A) Principal Coordinate Analysis of 10 fecal microbiomes showing differences in the distribution of taxonomic classifications between samples up to class level. B) Hierarchical clustering of 10 fecal microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram.