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Stool DNA Isolation Kit Dx

Cat. Dx27600
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Stool DNA Isolation Kit Dx

Cat. Dx27600

For the rapid and simple purification of bacterial and host DNA from stool and fecal samples for in vitro diagnostic use

  • CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
  • Ideal for use in in vitro diagnostic workflows
  • Simultaneous isolation of both host DNA and microbial DNA  (universal protocol)
  • Eliminates PCR inhibitors including humic acids
  • Fully compatible with Norgen's Stool Nucleic Acid Collection and Transport Tubes
  • High quality DNA for sensitive downstream applications including PCR, qPCR, sequencing and microarray

Note: This product is not available for sale in the United States

Research versions available here

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Stool DNA Isolation Kit Dx
(Cat. Dx27600)
50 preps

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. Dx27600
Kit Specifications
Maximum Stool Input
200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed
Frozen, fresh or preserved stool
Format Spin Column
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Elution Volume 50 μL
Time to Complete 10 Purifications
30 minutes
Applications

PCR, qPCR, Southern Blot Analysis,
Sequencing, Microarray Analysis. 


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Click for expanded view

Title Changes on fecal microbiota in rats exposed to permethrin during postnatal development
Journal Environmental Science and Pollution Research International. 2016.
Authors Nasuti C, Coman MM, Olek RA, Fiorini D, Verdenelli MC, Cecchini C, Silvi S, Fedeli D, Gabbianelli R
Title An Independent Evaluation of the CryoXtract Instruments' CXT350 Frozen Sample Aliquotter Using Tissue and Fecal Biospecimens
Journal Biopreservation and Biobanking. 2016.
Authors Mathieson W, Sanchez I, Mommaerts K, Frasquilho S, Betsou F
Title Cryptosporidiosis outbreak in a child day-care center caused by an unusual Cryptosporidium hominis subtype
Journal Enfermedades Infecciosas y Microbiología Clínica. 2015.
Authors Pilar Goñi, Diego Almagro-Nievas, Joanna Cieloszyk, Silvia Lóbez, José María Navarro-Marí, José Gutiérrez-Fernández
Title Detection of Campylobacter in human faecal samples in Fiji
Journal Western Pacific Surveillance and Response Journal. 2014.
Authors Aruna Devi, Jenny Wilkinson, Timothy Mahony and Thiru Vanniasinkam
Title How to make DNA count: DNA-based diagnostic tools in veterinary parasitology.
Journal Veterinary Parasitology. 2011.
Authors Hunt PW, Lello J.

Stool DNA Isolation Kit Dx

This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Devices Dx (Cat. Dx45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and other inhibitors using Bead Tubes and a combination of chemical and physical homogenization and lysis, without the need of any phenol-chloroform extractions. A simple and rapid spin column procedure is then used to further purify the DNA with high yields and molecular weights of up to 50 kb plus.  The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.

NOTE: This product is not available for sale in the United States.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. Dx27600
Kit Specifications
Maximum Stool Input
200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed
Frozen, fresh or preserved stool
Format Spin Column
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Elution Volume 50 μL
Time to Complete 10 Purifications
30 minutes
Applications

PCR, qPCR, Southern Blot Analysis,
Sequencing, Microarray Analysis. 


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Title Changes on fecal microbiota in rats exposed to permethrin during postnatal development
Journal Environmental Science and Pollution Research International. 2016.
Authors Nasuti C, Coman MM, Olek RA, Fiorini D, Verdenelli MC, Cecchini C, Silvi S, Fedeli D, Gabbianelli R
Title An Independent Evaluation of the CryoXtract Instruments' CXT350 Frozen Sample Aliquotter Using Tissue and Fecal Biospecimens
Journal Biopreservation and Biobanking. 2016.
Authors Mathieson W, Sanchez I, Mommaerts K, Frasquilho S, Betsou F
Title Cryptosporidiosis outbreak in a child day-care center caused by an unusual Cryptosporidium hominis subtype
Journal Enfermedades Infecciosas y Microbiología Clínica. 2015.
Authors Pilar Goñi, Diego Almagro-Nievas, Joanna Cieloszyk, Silvia Lóbez, José María Navarro-Marí, José Gutiérrez-Fernández
Title Detection of Campylobacter in human faecal samples in Fiji
Journal Western Pacific Surveillance and Response Journal. 2014.
Authors Aruna Devi, Jenny Wilkinson, Timothy Mahony and Thiru Vanniasinkam
Title How to make DNA count: DNA-based diagnostic tools in veterinary parasitology.
Journal Veterinary Parasitology. 2011.
Authors Hunt PW, Lello J.

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