Stool DNA Isolation Kits For the rapid and simple purification of bacterial and host DNA from stool and fecal samples. For research use only and NOT intended for in vitro diagnostics.CE-IVDR marked diagnostic version available here Powered by Bioz Stool DNA Isolation Kits For the rapid and simple purification of bacterial and host DNA from stool and fecal samples. Price USD$285.00 SKU 27600 Format Spin Column High Throughput Magnetic Bead System High Throughput Magnetic Bead System Size 50 Preps Quantity Request sample Overview Details Automation Documentation Citations Overview Simultaneous isolation of both host DNA and microbial DNA (universal protocol) Fully compatible with Norgen's Stool Nucleic Acid Collection and Transport Tubes High quality DNA for sensitive downstream applications including PCR, qPCR, Sequencing and microarray 96-Well and Magnetic Bead System formats also available These kits provide a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. Purifies the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA can be used with a number of downstream applications. Stool DNA Isolation Kit (Spin Column) Universal method to detect microorganism and host cell DNA simultaneously in stool samples. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process. Stool DNA Isolation 96-Well Kit (High Throughput) Fast and easy high throughput processing using centrifugation. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process. Stool DNA Isolation Kit (Magnetic Bead System) Fast and easy processing using a magnetic bead system. Robust lysis system (chemical lysis combined with a mechanical homogenization). Stool DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System) This kit provides fast and easy processing using a magnetic bead system, and a robust lysis system (chemical lysis combined with a mechanical homogenization). High throughput and compatible with an automation robotic system. Details Supporting Data Previous Figure 1. Higher Yields of DNA than Competitor Q. Stool DNA was isolated from 200 mg of fresh or preserved stool samples using Norgen’s Stool DNA Isolation Kit and Competitor Q's Kit. For evaluation, 10 µL of DNA from the elution was run on 1X TAE 1.2% agarose gel. Norgen's kit isolated much higher yields of DNA. *Stool was collected using Norgen’s Stool Nucleic Acid Collection and Preservation tubes (Cat. 45660). Marker = Norgen’s HighRanger DNA Ladder (Cat. 11900). Figure 2. Stool DNA quality and concentration measured by NanoDrop. High DNA concentration and quality was obtained using Norgen’s Stool DNA Isolation Kit from fresh or preserved stool samples. Figure 2A: Fresh stool samples, Figure 2B: Preserved stool using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). Figure 3. Detection of 16S rRNA from stool DNA isolated from 200 mg of fresh stool samples using Norgen's Stool DNA Isolation Kit and Competitor Q’s kit. DNA quality was confirmed by Real-time PCR using 2 µL of stool DNA (total PCR reaction volume was 20 µL) to detect 16S rRNA from different microorganisms in the stool samples. The earlier Ct value with Norgen's DNA samples (blue lines) compared to Competitor Q’s samples (red lines) indicated a higher quality of stool DNA for downstream applications. Figure 3A: Fresh stool sample, Figure 3B: Preserved stool using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). Figure 4. Detection of 5S rRNA from the Stool DNA isolated from 200 mg of fresh stool samples using Norgen's Stool DNA Isolation Kit and Competitor Q’s Kit. DNA quality was confirmed by Real-time PCR using 2 µL of stool DNA (total PCR reaction volume was 20 µL) to detect 5S rRNA from eukaryotic DNA in the stool samples. The earlier Ct value with Norgen's DNA samples (blue lines) compared to Competitor Q’s samples (red lines) indicated a higher quality of stool DNA for downstream applications. Figure 4A: Fresh stool sample, Figure 4B: Preserved stool using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). Figure 5. Better 16S rRNA detection from DNA isolated using Norgen’s Stool DNA Preservative and Stool DNA Isolation Kit. Two microlitres of stool DNA was used in a 20 µL PCR reaction volume. Stool was preserved using Norgen’s Stool DNA Preservative and isolated using Norgen’s Stool DNA Isolation Kit showed better 16S rRNA gene detection as compared to fresh stool isolated with Competitor Q. Figure 6. Distribution of 10 Different Fecal Microbiomes. A) Principal Coordinate Analysis of 10 fecal microbiomes showing differences in the distribution of taxonomic classifications between samples up to class level. B) Hierarchical clustering of 10 fecal microbiomes based on genus-level classifications, including a bar chart showing the relative abundance of genus-level classifications for each sample in the dendrogram. Figure 7. High Quality DNA Isolated from Preserved Stool Samples. DNA was isolated from 200 µL preserved stool samples using Norgen’s Stool DNA Isolation Kit (Magnetic Bead System).For evaluation, 10 µL from 75 µL of elution were run on 1X TAE 1.2% agarose gel. Stool samples were preserved in Norgen’s Stool Nucleic Acid Collection and Transport Tubes (Cat. 45630, 45660). M = Norgen’s HighRanger DNA Ladder (Cat. 11900). Figure 8. Comparison of A260/280 and A260/230 ratios. DNA was isolated from 200 mg stool samples using Norgen's Stool DNA Isolation Kit (Magnetic Bead System) and Norgen's Stool DNA Isolation Kit (column format). The purified DNA was then compared for A260/280 and A260/230 ratios. DNA isolated using Norgen’s Stool DNA Isolation Kit (Magnetic Bead System) (A, B and C) showed a comparable DNA quality to the DNA isolated using Norgen’s Stool DNA Isolation (Column method), indicating the consistence and highest DNA quality were generated by the Stool DNA Isolation Kit (Magnetic Bead System). Figure 9. High Quality DNA confirmed by Real-time PCR. DNA was isolated from 200 mg stool using Norgen's Stool DNA Isolation Kit (Magnetic Bead System) and Norgen's Stool DNA Isolation Kit (column format). The quality of the purified DNA was evaluated by using 8 μL of stool DNA (total PCR reaction volume was 20 µL) to detect 16S rRNA from the different stool samples. No PCR inhibition was observed from DNA isolated using Norgen's Stool DNA Isolation Kit (Magnetic Bead System) (blue circles), similar to the DNA isolated using Norgen’s Stool DNA Isolation Kit (Column method) (red circles), indicating the excellent isolation consistency and the quality of the stool DNA for downstream applications. Figure 10. 16S Metagenomics data generated by Illumina MiSeq. Stool DNA was isolated using Norgen’s Stool DNA Isolation Kit (Magnetic Bead System) from 200 mg of stool from healthy donors, and the stool microbiomes were successfully sequenced by Illumina MiSeq. Figure 11. High Quality DNA Isolated from Preserved Stool Samples. DNA was isolated from 200 µL preserved stool samples using Norgen’s Stool DNA Isolation 96-Well Kit (Magnetic Bead System). For evaluation, 10 µL from 75 µL of elution were run on 1X TAE 1.2% agarose gel. M = Norgen’s HighRanger DNA Ladder (Cat. 11900). Figure 12. High Quality DNA Confirmed by Real-Time PCR. DNA was isolated from 200 mg stool using Norgen's Stool DNA Isolation 96-Well Kit (Magnetic Bead System). The quality of the purified DNA was evaluated by using 2 µL of stool DNA (total PCR reaction volume was 20 µL) to detect 16S rRNA from the stool sample. No PCR inhibition was observed from DNA isolated using Norgen's Stool DNA Isolation 96-Well Kit (Magnetic Bead System) indicating the excellent isolation consistency and the high quality of the stool DNA for downstream applications. Next Click for expanded view Kit Specifications Maximum Stool Input 200 mg (fresh/frozen stool) or 400 μL (preserved stool) Type of Stool Processed Frozen, fresh or preserved stool Format Spin Column Maximum Column Binding Capacity 50 μg Maximum Column Loading Volume 650 μL Elution Volume 50 μL Time to Complete 10 Purifications 30 minutes Applications PCR, qPCR, Southern Blot Analysis, Sequencing, Microarray Analysis. Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 27600 (50 preps) Cat. 65600 (192 preps) Cat. 55700 (50 preps) Cat. 63100 (192 preps) Lysis Buffer L 60 mL 2 x 105 mL 60 mL 3 x 60 mL1 x 30 mL Lysis Additive A 6 mL 25 mL 6 mL 25 mL Binding Buffer I 7 mL 25 mL 7 mL 25 mL Binding Buffer C 30 mL 110 mL - - Wash Solution A 18 mL 2 x 38 mL - - Magnetic Bead Suspension - - 1.1 mL 4 x 1.1 mL Solution WN - - 18 mL 55 mL Elution Buffer B 8 mL 30 mL 8 mL 30 mL Bead Tubes 50 200 50 196 Mini Spin Columns 50 - - - 96-Well Plate - 2 - 2 Adhesive Tape - 4 - 2 Collection Tubes 50 - - - 96-Well Collection Plate - 2 - - Elution Tubes (1.7 mL) 50 - 50 - 96-Well Elution Plate - 2 - 2 Product Insert 1 1 1 1 Automation Stool DNA Automated Isolation Tutorial Supplementary Protocol Supplementary Protocol - Automated Procedure for Stool DNA Isolation Kit (Magnetic Bead System) Documentation PROTOCOLS (27600) Stool DNA Isolation Kit (Spin Column) - Protocol (50 prep) (65600) Stool DNA Isolation 96-Well Kit (High Throughput) - Protocol (2x96-Well) (55700) Stool DNA Isolation Kit (Magnetic Bead System) - Protocol (50 prep) (63100) Stool DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System) - Protocol (2 x 96-well) SUPPLEMENTARY PROTOCOLS Supplementary Protocol - Automated Procedure for Stool DNA Isolation Kit (Magnetic Bead System) APPLICATION NOTES Comparative Study of DNA Isolated from Stool Using Norgen’s Stool Nucleic Acid Isolation Kit and Norgen’s Stool DNA Isolation Mi Detection of Viral, Bacterial and Human Genomic DNA from Preserved Stool Samples The Isolation of High Quality Stool DNA and its Application to Quantitative Adenovirus Detection using TaqMan® Real-Time PCR Comparison of Stool DNA Isolation Methods for Bacterial and Mammalian DNA Detection Determination of the DNA Molecular Weight (MW) from different Norgen Columns and Isolation Methods Immediate Viral Inactivation Using Norgen’s Stool Nucleic Acid Preservative Gut Microbiome Diversity: Comparison of Stool DNA Preservation Methods Comparative Microbiome Profiles from Fecal Preservation Methods Automation of Stool DNA Isolation on the Hamilton Vantage Using Norgen’s Magnetic Bead Stool Isolation Kit SAFETY DATA SHEETS (27600) Stool DNA Isolation Kit - Safety Data Sheet (55700, 63100) Stool DNA Isolation Kit (Magnetic Bead System) - Safety Data Sheet POSTERS Viability Of Microorganisms in Saliva & Stool Collection and Transportation Preservatives FLYERS Flyer Stool Magentic Bead 6.0.pdf Citations Powered by Bioz See more details on Bioz Related Products Stool Nucleic Acid Isolation Kit Stool Total RNA Purification Kit Stool DNA Isolation Kit (Magnetic Bead System) Fecal DNA Collection & Preservation Mini Tubes
Stool DNA Isolation Kits For the rapid and simple purification of bacterial and host DNA from stool and fecal samples. For research use only and NOT intended for in vitro diagnostics.CE-IVDR marked diagnostic version available here Powered by Bioz Stool DNA Isolation Kits For the rapid and simple purification of bacterial and host DNA from stool and fecal samples. Price USD$285.00 SKU 27600 Format Spin Column High Throughput Magnetic Bead System High Throughput Magnetic Bead System Size 50 Preps Quantity Request sample