A convenient and rapid method to purify total RNA from small amounts of stool samples for in vitro diagnostic use
Note: This product is not available for sale in the United States
|Stool Total RNA Purification Kit Dx||Dx49500||50 preps|
Stool Total RNA Purification Kit Dx
This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen's Stool Nucleic Acid Collection and Transport Devices Dx (Cat. Dx45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes.
NOTE: This product is not available for sale in the United States.
Maximum Stool Input
Type of Stool Processed
Frozen and fresh stool
Maximum Column Binding Capacity
|Maximum Column Loading Volume||
|Time to Complete 10 Purifications||
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
|Title||The role of whole-grain barley on human fecal microbiota and metabolome|
|Journal||Applied and Environmental Microbiology. 2015.|
|Authors||Maria De Angelis, Eustacchio Montemurno, Lucia Vannini, Carmela Cosola, Noemi Cavallo, Giorgia Gozzi, Valentina Maranzano, Raffaella Di Cagno, Marco Gobbettia and Loreto Gesualdo|
|Title||Microbiota and metabolome of un-started and started Greek-type fermentation of Bella di Cerignola table olives|
|Journal||Food Microbiology. 2015.|
|Authors||Maria De Angelis, Daniela Campanella, Lucrezia Cosmai, Carmine Summo, Carlo Giuseppe Rizzello, Francesco Caponio|
|Title||Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation|
|Journal||BMC Genomics. 2015.|
|Authors||Michael Reck, Jürgen Tomasch, Zhiluo Deng, Michael Jarek, Peter Husemann, Irene Wagner-Döbler|
|Title||Fecal Microbiota in Healthy Subjects Following Omnivore, Vegetarian and Vegan Diets: Culturable Populations and rRNA DGGE Profiling|
|Journal||PLOS ONE. 2015.|
|Authors||Ilario Ferrocino, Raffaella Di Cagno, Maria De Angelis, Silvia Turroni, Lucia Vannini, Elena Bancalari, Kalliopi Rantsiou, Gianluigi Cardinali, Erasmo Neviani, Luca Cocolin|
|Title||Modulation of the Intestinal Microbiota Is Associated with Lower Plasma Cholesterol and Weight Gain in Hamsters Fed Chardonnay Grape Seed Flour.|
|Journal||Journal of Agriculture and Food Chemistry. 2015.|
|Authors||Hyunsook Kim, Dong-Hyeon Kim, Kun-ho Seo, Jung-Whan Chon, Seung-Yeol Nah, Glenn E. Bartley, Torey Arvik, Rebecca Lipson, and Wallace Yokoyama.|
|Title||Microbiota and Metabolome Associated with Immunoglobulin A Nephropathy (IgAN).|
|Journal||Microbiota and Metabolome of IgA Nephropathy Patients. 2014.|
|Authors||Maria De Angelis, Eustacchio Montemurno, Maria Piccolo, Lucia Vannini, Gabriella Lauriero, Valentina Maranzano, Giorgia Gozzi, Diana Serrazanetti, Giuseppe Dalfino, Marco Gobbetti, Loreto Gesualdo.|
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