A convenient and rapid method to purify total RNA from small amounts of stool samples

  • Simultaneous isolation of both host RNA and microbial RNA (universal protocol)
  • Isolate full diversity of RNA from large RNA down to small and microRNAs
  • Eliminates PCR inhibitors including humic acids
  • High quality RNA for sensitive downstream applications

ItemsCat. #Size
Stool Total RNA Purification Kit 49500 50 preps

Stool Total RNA Purification Kit

This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen's Stool Nucleic Acid Collection and Transport Tubes. The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes.

Kit Specifications
Maximum Stool Input
200 mg
Type of Stool Processed
Frozen and fresh stool
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Time to Complete 10 Purifications
30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Kit Components
Component Cat. 49500 (50 preps)
Lysis Buffer C 60 mL
Wash Solution A 38 mL
Elution Buffer E 6 mL
Bead Tubes 50
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title The role of whole-grain barley on human fecal microbiota and metabolome
Journal Applied and Environmental Microbiology. 2015.
Authors Maria De Angelis, Eustacchio Montemurno, Lucia Vannini, Carmela Cosola, Noemi Cavallo, Giorgia Gozzi, Valentina Maranzano, Raffaella Di Cagno, Marco Gobbettia and Loreto Gesualdo
Title Microbiota and metabolome of un-started and started Greek-type fermentation of Bella di Cerignola table olives
Journal Food Microbiology. 2015.
Authors Maria De Angelis, Daniela Campanella, Lucrezia Cosmai, Carmine Summo, Carlo Giuseppe Rizzello, Francesco Caponio
Title Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation
Journal BMC Genomics. 2015.
Authors Michael Reck, Jürgen Tomasch, Zhiluo Deng, Michael Jarek, Peter Husemann, Irene Wagner-Döbler
Title Fecal Microbiota in Healthy Subjects Following Omnivore, Vegetarian and Vegan Diets: Culturable Populations and rRNA DGGE Profiling
Journal PLOS ONE. 2015.
Authors Ilario Ferrocino, Raffaella Di Cagno, Maria De Angelis, Silvia Turroni, Lucia Vannini, Elena Bancalari, Kalliopi Rantsiou, Gianluigi Cardinali, Erasmo Neviani, Luca Cocolin
Title Modulation of the Intestinal Microbiota Is Associated with Lower Plasma Cholesterol and Weight Gain in Hamsters Fed Chardonnay Grape Seed Flour.
Journal Journal of Agriculture and Food Chemistry. 2015.
Authors Hyunsook Kim, Dong-Hyeon Kim, Kun-ho Seo, Jung-Whan Chon, Seung-Yeol Nah, Glenn E. Bartley, Torey Arvik, Rebecca Lipson, and Wallace Yokoyama.
Title Microbiota and Metabolome Associated with Immunoglobulin A Nephropathy (IgAN).
Journal Microbiota and Metabolome of IgA Nephropathy Patients. 2014.
Authors Maria De Angelis, Eustacchio Montemurno, Maria Piccolo, Lucia Vannini, Gabriella Lauriero, Valentina Maranzano, Giorgia Gozzi, Diana Serrazanetti, Giuseppe Dalfino, Marco Gobbetti, Loreto Gesualdo.