A convenient and rapid method to purify total RNA from small amounts of stool samples

  • Simultaneous isolation of both host RNA and microbial RNA (universal protocol)
  • Isolate full diversity of RNA from large RNA down to small and microRNAs
  • Eliminates PCR inhibitors including humic acids
  • High quality RNA for sensitive downstream applications

ItemsCat. #Size
Stool Total RNA Purification Kit 49500 50 preps

Stool Total RNA Purification Kit

This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen's Stool Nucleic Acid Collection and Transport Tubes (Cat. 45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes.

Kit Specifications
Maximum Stool Input
 200 mg (fresh or frozen stool)
Type of Stool Processed
 All types of feces from humans and animals 
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
600 μL
Time to Complete 10 Purifications
30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

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Journal Journal of Dairy Science. 2016.
Authors Lopez CC, Serio A, Rossi C, Mazzarrino G, Marchetti S, Castellani F, Grotta L, Fiorentino FP, Paparella A, Martino G
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Journal Food Microbiology. 2015.
Authors Maria De Angelis, Daniela Campanella, Lucrezia Cosmai, Carmine Summo, Carlo Giuseppe Rizzello, Francesco Caponio
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Journal BMC Genomics. 2015.
Authors Michael Reck, Jürgen Tomasch, Zhiluo Deng, Michael Jarek, Peter Husemann, Irene Wagner-Döbler
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Journal PLOS ONE. 2015.
Authors Ilario Ferrocino, Raffaella Di Cagno, Maria De Angelis, Silvia Turroni, Lucia Vannini, Elena Bancalari, Kalliopi Rantsiou, Gianluigi Cardinali, Erasmo Neviani, Luca Cocolin
Title Modulation of the Intestinal Microbiota Is Associated with Lower Plasma Cholesterol and Weight Gain in Hamsters Fed Chardonnay Grape Seed Flour.
Journal Journal of Agriculture and Food Chemistry. 2015.
Authors Hyunsook Kim, Dong-Hyeon Kim, Kun-ho Seo, Jung-Whan Chon, Seung-Yeol Nah, Glenn E. Bartley, Torey Arvik, Rebecca Lipson, and Wallace Yokoyama.
Title Microbiota and Metabolome Associated with Immunoglobulin A Nephropathy (IgAN).
Journal Microbiota and Metabolome of IgA Nephropathy Patients. 2014.
Authors Maria De Angelis, Eustacchio Montemurno, Maria Piccolo, Lucia Vannini, Gabriella Lauriero, Valentina Maranzano, Giorgia Gozzi, Diana Serrazanetti, Giuseppe Dalfino, Marco Gobbetti, Loreto Gesualdo.