*Inquire by phone or email for more information.
A convenient and rapid method to purify total RNA from small amounts of stool samples.
For research use only and NOT intended for in vitro diagnostics.
Diagnostic version available here
Want a discount?
Create your account to receive 15% off on your first purchase!
Kit Specifications
Kit Specifications
|
|
Maximum Stool Input
|
200 mg (fresh/frozen stool) or 400 μL (preserved stool)
|
Type of Stool Processed
|
Preserved, fresh, and frozen stool from humans and animals
|
Maximum Column Binding Capacity
|
50 μg
|
Maximum Column Loading Volume |
600 μL
|
Time to Complete 10 Purifications |
30 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Supporting Data
Title | Metagenomic analysis of colorectal cancer datasets identifies cross-cohort microbial diagnostic signatures and a link with choline degradation |
Journal | NATURE MEDICINE.. 2022. |
Authors | Andrew Maltez Thomas, Paolo Manghi, Francesco Asnicar, Edoardo Pasolli, Federica Armanini, Moreno Zolfo, Francesco Beghini, Serena Manara, Nicolai Karcher,Chiara Pozzi, Sara Gandini, Davide Serrano, Sonia Tarallo, Antonio Francavilla,Gaetano Gallo, et al. |
Title | The 8q24 region hosts miRNAs altered in biospecimens of colorectal and bladder cancer patients |
Journal | Cancer Medicine. 2022. |
Authors | Amedeo Gagliardi,Giulia Francescato,Giulio Ferrero,Giovanni Birolo,Sonia Tarallo,Antonio Francavilla,Giulia Piaggeschi,Carla Di Battista,Gaetano Gallo,Alberto Realis Luc,Carlotta Sacerdote,Giuseppe Matullo,Paolo Vineis,Alessio Naccarati,Barbara Pardini |
Title | Altered Fecal Small RNA Profiles in Colorectal Cancer Reflect Gut Microbiome Composition in Stool Samples |
Journal | ASM Journals. 2019. |
Authors | Sonia Tarallo, Giulio Ferrero, Gaetano Gallo, Antonio Francavilla, Giuseppe Clerico, Alberto Realis Luc, Paolo Manghi, Francesca Cordero |
Title | Effect of diet supplementation with Ascophyllum nodosum on cow milk composition and microbiota |
Journal | Journal of Dairy Science. 2016. |
Authors | Lopez CC, Serio A, Rossi C, Mazzarrino G, Marchetti S, Castellani F, Grotta L, Fiorentino FP, Paparella A, Martino G |
Title | Multiplex reverse transcription quantitative PCR detection of a single-stranded RNA virus HcRNAV infecting the bloom-forming dinoflagellate Heterocapsa circularisquama |
Journal | Limnology and Oceanography. 2016. |
Authors | N Nakayama, M Hamaguchi |
Title | The role of whole-grain barley on human fecal microbiota and metabolome |
Journal | Applied and Environmental Microbiology. 2015. |
Authors | Maria De Angelis, Eustacchio Montemurno, Lucia Vannini, Carmela Cosola, Noemi Cavallo, Giorgia Gozzi, Valentina Maranzano, Raffaella Di Cagno, Marco Gobbettia and Loreto Gesualdo |
Title | Microbiota and metabolome of un-started and started Greek-type fermentation of Bella di Cerignola table olives |
Journal | Food Microbiology. 2015. |
Authors | Maria De Angelis, Daniela Campanella, Lucrezia Cosmai, Carmine Summo, Carlo Giuseppe Rizzello, Francesco Caponio |
Title | Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation |
Journal | BMC Genomics. 2015. |
Authors | Michael Reck, Jürgen Tomasch, Zhiluo Deng, Michael Jarek, Peter Husemann, Irene Wagner-Döbler |
Title | Fecal Microbiota in Healthy Subjects Following Omnivore, Vegetarian and Vegan Diets: Culturable Populations and rRNA DGGE Profiling |
Journal | PLOS ONE. 2015. |
Authors | Ilario Ferrocino, Raffaella Di Cagno, Maria De Angelis, Silvia Turroni, Lucia Vannini, Elena Bancalari, Kalliopi Rantsiou, Gianluigi Cardinali, Erasmo Neviani, Luca Cocolin |
Title | Modulation of the Intestinal Microbiota Is Associated with Lower Plasma Cholesterol and Weight Gain in Hamsters Fed Chardonnay Grape Seed Flour. |
Journal | Journal of Agriculture and Food Chemistry. 2015. |
Authors | Hyunsook Kim, Dong-Hyeon Kim, Kun-ho Seo, Jung-Whan Chon, Seung-Yeol Nah, Glenn E. Bartley, Torey Arvik, Rebecca Lipson, and Wallace Yokoyama. |
Title | Microbiota and Metabolome Associated with Immunoglobulin A Nephropathy (IgAN). |
Journal | Microbiota and Metabolome of IgA Nephropathy Patients. 2014. |
Authors | Maria De Angelis, Eustacchio Montemurno, Maria Piccolo, Lucia Vannini, Gabriella Lauriero, Valentina Maranzano, Giorgia Gozzi, Diana Serrazanetti, Giuseppe Dalfino, Marco Gobbetti, Loreto Gesualdo. |
Stool Total RNA Purification Kit
This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen's Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes for 10 samples.
Supporting Data
Kit Specifications
Kit Specifications
|
|
Maximum Stool Input
|
200 mg (fresh/frozen stool) or 400 μL (preserved stool)
|
Type of Stool Processed
|
Preserved, fresh, and frozen stool from humans and animals
|
Maximum Column Binding Capacity
|
50 μg
|
Maximum Column Loading Volume |
600 μL
|
Time to Complete 10 Purifications |
30 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Title | Metagenomic analysis of colorectal cancer datasets identifies cross-cohort microbial diagnostic signatures and a link with choline degradation |
Journal | NATURE MEDICINE.. 2022. |
Authors | Andrew Maltez Thomas, Paolo Manghi, Francesco Asnicar, Edoardo Pasolli, Federica Armanini, Moreno Zolfo, Francesco Beghini, Serena Manara, Nicolai Karcher,Chiara Pozzi, Sara Gandini, Davide Serrano, Sonia Tarallo, Antonio Francavilla,Gaetano Gallo, et al. |
Title | The 8q24 region hosts miRNAs altered in biospecimens of colorectal and bladder cancer patients |
Journal | Cancer Medicine. 2022. |
Authors | Amedeo Gagliardi,Giulia Francescato,Giulio Ferrero,Giovanni Birolo,Sonia Tarallo,Antonio Francavilla,Giulia Piaggeschi,Carla Di Battista,Gaetano Gallo,Alberto Realis Luc,Carlotta Sacerdote,Giuseppe Matullo,Paolo Vineis,Alessio Naccarati,Barbara Pardini |
Title | Altered Fecal Small RNA Profiles in Colorectal Cancer Reflect Gut Microbiome Composition in Stool Samples |
Journal | ASM Journals. 2019. |
Authors | Sonia Tarallo, Giulio Ferrero, Gaetano Gallo, Antonio Francavilla, Giuseppe Clerico, Alberto Realis Luc, Paolo Manghi, Francesca Cordero |
Title | Effect of diet supplementation with Ascophyllum nodosum on cow milk composition and microbiota |
Journal | Journal of Dairy Science. 2016. |
Authors | Lopez CC, Serio A, Rossi C, Mazzarrino G, Marchetti S, Castellani F, Grotta L, Fiorentino FP, Paparella A, Martino G |
Title | Multiplex reverse transcription quantitative PCR detection of a single-stranded RNA virus HcRNAV infecting the bloom-forming dinoflagellate Heterocapsa circularisquama |
Journal | Limnology and Oceanography. 2016. |
Authors | N Nakayama, M Hamaguchi |
Title | The role of whole-grain barley on human fecal microbiota and metabolome |
Journal | Applied and Environmental Microbiology. 2015. |
Authors | Maria De Angelis, Eustacchio Montemurno, Lucia Vannini, Carmela Cosola, Noemi Cavallo, Giorgia Gozzi, Valentina Maranzano, Raffaella Di Cagno, Marco Gobbettia and Loreto Gesualdo |
Title | Microbiota and metabolome of un-started and started Greek-type fermentation of Bella di Cerignola table olives |
Journal | Food Microbiology. 2015. |
Authors | Maria De Angelis, Daniela Campanella, Lucrezia Cosmai, Carmine Summo, Carlo Giuseppe Rizzello, Francesco Caponio |
Title | Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation |
Journal | BMC Genomics. 2015. |
Authors | Michael Reck, Jürgen Tomasch, Zhiluo Deng, Michael Jarek, Peter Husemann, Irene Wagner-Döbler |
Title | Fecal Microbiota in Healthy Subjects Following Omnivore, Vegetarian and Vegan Diets: Culturable Populations and rRNA DGGE Profiling |
Journal | PLOS ONE. 2015. |
Authors | Ilario Ferrocino, Raffaella Di Cagno, Maria De Angelis, Silvia Turroni, Lucia Vannini, Elena Bancalari, Kalliopi Rantsiou, Gianluigi Cardinali, Erasmo Neviani, Luca Cocolin |
Title | Modulation of the Intestinal Microbiota Is Associated with Lower Plasma Cholesterol and Weight Gain in Hamsters Fed Chardonnay Grape Seed Flour. |
Journal | Journal of Agriculture and Food Chemistry. 2015. |
Authors | Hyunsook Kim, Dong-Hyeon Kim, Kun-ho Seo, Jung-Whan Chon, Seung-Yeol Nah, Glenn E. Bartley, Torey Arvik, Rebecca Lipson, and Wallace Yokoyama. |
Title | Microbiota and Metabolome Associated with Immunoglobulin A Nephropathy (IgAN). |
Journal | Microbiota and Metabolome of IgA Nephropathy Patients. 2014. |
Authors | Maria De Angelis, Eustacchio Montemurno, Maria Piccolo, Lucia Vannini, Gabriella Lauriero, Valentina Maranzano, Giorgia Gozzi, Diana Serrazanetti, Giuseppe Dalfino, Marco Gobbetti, Loreto Gesualdo. |