Stool Total RNA Purification Kit

Cat. 49500
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Stool Total RNA Purification Kit

Cat. 49500

A convenient and rapid method to purify total RNA from small amounts of stool samples.

  • Simultaneous isolation of both host RNA and microbial RNA (universal protocol)
  • Isolate full diversity of RNA from large RNA down to small and microRNAs
  • Eliminates PCR inhibitors including humic acids
  • High quality RNA for sensitive downstream application
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Stool Total RNA Purification Kit
(Cat. 49500)
50 preps

Kit Size

  • Simultaneous isolation of both host RNA and microbial RNA (universal protocol)
  • Isolate full diversity of RNA from large RNA down to small and microRNAs
  • Eliminates PCR inhibitors including humic acids
  • High quality RNA for sensitive downstream application
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Stool Total RNA Purification Kit

This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen's Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes for 10 samples.

Kit Specifications

You have selected: Cat. 49500
Kit Specifications
Maximum Stool Input
 200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed
 Preserved, fresh, and frozen stool from humans and animals 
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
600 μL
Time to Complete 10 Purifications
30 minutes


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Click for expanded view

Component Cat. 49500 (50 preps)
Lysis Buffer C 60 mL
Wash Solution A 38 mL
Elution Buffer E 6 mL
Bead Tubes 50
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Metagenomic analysis of colorectal cancer datasets identifies cross-cohort microbial diagnostic signatures and a link with choline degradation
Journal NATURE MEDICINE.. 2022.
Authors Andrew Maltez Thomas, Paolo Manghi, Francesco Asnicar, Edoardo Pasolli, Federica Armanini, Moreno Zolfo, Francesco Beghini, Serena Manara, Nicolai Karcher,Chiara Pozzi, Sara Gandini, Davide Serrano, Sonia Tarallo, Antonio Francavilla,Gaetano Gallo, et al.
Title The 8q24 region hosts miRNAs altered in biospecimens of colorectal and bladder cancer patients
Journal Cancer Medicine. 2022.
Authors Amedeo Gagliardi,Giulia Francescato,Giulio Ferrero,Giovanni Birolo,Sonia Tarallo,Antonio Francavilla,Giulia Piaggeschi,Carla Di Battista,Gaetano Gallo,Alberto Realis Luc,Carlotta Sacerdote,Giuseppe Matullo,Paolo Vineis,Alessio Naccarati,Barbara Pardini
Title Altered Fecal Small RNA Profiles in Colorectal Cancer Reflect Gut Microbiome Composition in Stool Samples
Journal ASM Journals. 2019.
Authors Sonia Tarallo, Giulio Ferrero, Gaetano Gallo, Antonio Francavilla, Giuseppe Clerico, Alberto Realis Luc, Paolo Manghi, Francesca Cordero
Title Effect of diet supplementation with Ascophyllum nodosum on cow milk composition and microbiota
Journal Journal of Dairy Science. 2016.
Authors Lopez CC, Serio A, Rossi C, Mazzarrino G, Marchetti S, Castellani F, Grotta L, Fiorentino FP, Paparella A, Martino G
Title The role of whole-grain barley on human fecal microbiota and metabolome
Journal Applied and Environmental Microbiology. 2015.
Authors Maria De Angelis, Eustacchio Montemurno, Lucia Vannini, Carmela Cosola, Noemi Cavallo, Giorgia Gozzi, Valentina Maranzano, Raffaella Di Cagno, Marco Gobbettia and Loreto Gesualdo
Title Microbiota and metabolome of un-started and started Greek-type fermentation of Bella di Cerignola table olives
Journal Food Microbiology. 2015.
Authors Maria De Angelis, Daniela Campanella, Lucrezia Cosmai, Carmine Summo, Carlo Giuseppe Rizzello, Francesco Caponio
Title Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation
Journal BMC Genomics. 2015.
Authors Michael Reck, Jürgen Tomasch, Zhiluo Deng, Michael Jarek, Peter Husemann, Irene Wagner-Döbler
Title Fecal Microbiota in Healthy Subjects Following Omnivore, Vegetarian and Vegan Diets: Culturable Populations and rRNA DGGE Profiling
Journal PLOS ONE. 2015.
Authors Ilario Ferrocino, Raffaella Di Cagno, Maria De Angelis, Silvia Turroni, Lucia Vannini, Elena Bancalari, Kalliopi Rantsiou, Gianluigi Cardinali, Erasmo Neviani, Luca Cocolin
Title Modulation of the Intestinal Microbiota Is Associated with Lower Plasma Cholesterol and Weight Gain in Hamsters Fed Chardonnay Grape Seed Flour.
Journal Journal of Agriculture and Food Chemistry. 2015.
Authors Hyunsook Kim, Dong-Hyeon Kim, Kun-ho Seo, Jung-Whan Chon, Seung-Yeol Nah, Glenn E. Bartley, Torey Arvik, Rebecca Lipson, and Wallace Yokoyama.
Title Microbiota and Metabolome Associated with Immunoglobulin A Nephropathy (IgAN).
Journal Microbiota and Metabolome of IgA Nephropathy Patients. 2014.
Authors Maria De Angelis, Eustacchio Montemurno, Maria Piccolo, Lucia Vannini, Gabriella Lauriero, Valentina Maranzano, Giorgia Gozzi, Diana Serrazanetti, Giuseppe Dalfino, Marco Gobbetti, Loreto Gesualdo.

Stool Total RNA Purification Kit

This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen's Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes for 10 samples.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 49500
Kit Specifications
Maximum Stool Input
 200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed
 Preserved, fresh, and frozen stool from humans and animals 
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
600 μL
Time to Complete 10 Purifications
30 minutes


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component Cat. 49500 (50 preps)
Lysis Buffer C 60 mL
Wash Solution A 38 mL
Elution Buffer E 6 mL
Bead Tubes 50
Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Metagenomic analysis of colorectal cancer datasets identifies cross-cohort microbial diagnostic signatures and a link with choline degradation
Journal NATURE MEDICINE.. 2022.
Authors Andrew Maltez Thomas, Paolo Manghi, Francesco Asnicar, Edoardo Pasolli, Federica Armanini, Moreno Zolfo, Francesco Beghini, Serena Manara, Nicolai Karcher,Chiara Pozzi, Sara Gandini, Davide Serrano, Sonia Tarallo, Antonio Francavilla,Gaetano Gallo, et al.
Title The 8q24 region hosts miRNAs altered in biospecimens of colorectal and bladder cancer patients
Journal Cancer Medicine. 2022.
Authors Amedeo Gagliardi,Giulia Francescato,Giulio Ferrero,Giovanni Birolo,Sonia Tarallo,Antonio Francavilla,Giulia Piaggeschi,Carla Di Battista,Gaetano Gallo,Alberto Realis Luc,Carlotta Sacerdote,Giuseppe Matullo,Paolo Vineis,Alessio Naccarati,Barbara Pardini
Title Altered Fecal Small RNA Profiles in Colorectal Cancer Reflect Gut Microbiome Composition in Stool Samples
Journal ASM Journals. 2019.
Authors Sonia Tarallo, Giulio Ferrero, Gaetano Gallo, Antonio Francavilla, Giuseppe Clerico, Alberto Realis Luc, Paolo Manghi, Francesca Cordero
Title Effect of diet supplementation with Ascophyllum nodosum on cow milk composition and microbiota
Journal Journal of Dairy Science. 2016.
Authors Lopez CC, Serio A, Rossi C, Mazzarrino G, Marchetti S, Castellani F, Grotta L, Fiorentino FP, Paparella A, Martino G
Title The role of whole-grain barley on human fecal microbiota and metabolome
Journal Applied and Environmental Microbiology. 2015.
Authors Maria De Angelis, Eustacchio Montemurno, Lucia Vannini, Carmela Cosola, Noemi Cavallo, Giorgia Gozzi, Valentina Maranzano, Raffaella Di Cagno, Marco Gobbettia and Loreto Gesualdo
Title Microbiota and metabolome of un-started and started Greek-type fermentation of Bella di Cerignola table olives
Journal Food Microbiology. 2015.
Authors Maria De Angelis, Daniela Campanella, Lucrezia Cosmai, Carmine Summo, Carlo Giuseppe Rizzello, Francesco Caponio
Title Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation
Journal BMC Genomics. 2015.
Authors Michael Reck, Jürgen Tomasch, Zhiluo Deng, Michael Jarek, Peter Husemann, Irene Wagner-Döbler
Title Fecal Microbiota in Healthy Subjects Following Omnivore, Vegetarian and Vegan Diets: Culturable Populations and rRNA DGGE Profiling
Journal PLOS ONE. 2015.
Authors Ilario Ferrocino, Raffaella Di Cagno, Maria De Angelis, Silvia Turroni, Lucia Vannini, Elena Bancalari, Kalliopi Rantsiou, Gianluigi Cardinali, Erasmo Neviani, Luca Cocolin
Title Modulation of the Intestinal Microbiota Is Associated with Lower Plasma Cholesterol and Weight Gain in Hamsters Fed Chardonnay Grape Seed Flour.
Journal Journal of Agriculture and Food Chemistry. 2015.
Authors Hyunsook Kim, Dong-Hyeon Kim, Kun-ho Seo, Jung-Whan Chon, Seung-Yeol Nah, Glenn E. Bartley, Torey Arvik, Rebecca Lipson, and Wallace Yokoyama.
Title Microbiota and Metabolome Associated with Immunoglobulin A Nephropathy (IgAN).
Journal Microbiota and Metabolome of IgA Nephropathy Patients. 2014.
Authors Maria De Angelis, Eustacchio Montemurno, Maria Piccolo, Lucia Vannini, Gabriella Lauriero, Valentina Maranzano, Giorgia Gozzi, Diana Serrazanetti, Giuseppe Dalfino, Marco Gobbetti, Loreto Gesualdo.

 


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