• Extract high quality & purity total RNA including miRNA 
  • No phenol step required - isolate all RNA in one fraction
  • Rapid processing in under 40 minutes 
  • Isolate total RNA from a wide variety of specimens

ItemsCat. #Size
Total RNA Purification Maxi Kit 26800 8 preps

Total RNA Purification Maxi Kit

Norgen’s Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen’s Purification Technology

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). Ethanol is then added to the lysate, and the solution is loaded onto a maxi spin-column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Solution A. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.

 

Kit Specifications
Maximum Column Binding Capacity
1.5 mg
Maximum Column Loading Volume
20 mL
Size of RNA Purified
All sizes, including small RNA
(< 200 nt)
Maximum Amount of Starting Material: Liver Tissue
Heart Tissue
Kidney Tissue
Brain Tissue
Lung Tissue
Whole Blood
Bacteria
Yeast
Fungi
Plant Tissues
10 mg
5 mg
10 mg
25 mg
10 mg
2 – 10 mL*
2.5 x 1010 cells
2 x 108 cells
1 g
1 g
Time to Complete 4 Purifications
40 minutes
Average Yield:
HeLa Cells (5 x 107 cells)
E. coli (2.5 x 1010 cells)
~750 μg
~1.5 mg

*For isolating total RNA from purified leukocytes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.