Total RNA Purification Maxi Kit For the rapid purification of total RNA - including microRNA - without the use of phenol For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Total RNA Purification Maxi Kit For the rapid purification of total RNA - including microRNA - without the use of phenol Price USD$175.00 SKU 26800 Format Maxi Size 8 Preps Quantity Overview Details Documentation Citations Overview Extract high quality & quantity total RNA including miRNA No phenol step required - isolate all RNA in one fraction Rapid processing in under 40 minutes Isolate total RNA from a wide variety of specimens Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants, and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. Norgen’s Purification Technology Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). Ethanol is then added to the lysate, and the solution is loaded onto a maxi spin column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Solution A. The purified RNA is of the highest integrity, and can be used in a number of downstream applications. Details Supporting Data Previous Figure 1. Isolate a Diversity of RNA Species. Total RNA was isolated from 10 mL of an overnight E. coli culture using Norgen's Total RNA Purification Maxi Kit. One microliter of the 2 mL elution was analyzed on an Agilent® 2100 BioAnalyzer RNA Nano 6000 chip. As it can be seen, high-quality total RNA was isolated with minimal degradation. As well, a large amount of small RNA (< 200 nt) was recovered, indicating that the kit is able to isolate a diversity of RNA species. Figure 2. Good Amplification and Consistency of Isolation. Norgen's Total RNA Purification Maxi Kit was used to isolate total RNA from six different overnight samples of E. coli (10 mL samples). In brief, one microgram of the isolated RNA was subjected to a 20 µL reverse transcription using Invitrogen Superscript III. Three microliters of the reverse transcription were used as the template in a 20 µL qPCR reaction using 16S rRNA E. coli specific primers and Bio-Rad iQ mix. As it can be seen, the RNA was detected and successfully amplified in all cases. Furthermore, all samples showed a similar Ct value, indicating consistent isolation. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity 1.5 mg Maximum Column Loading Volume 20 mL Size of RNA Purified All sizes, including small RNA (< 200 nt) Maximum Amount of Starting Material Liver Tissue 100 mg Heart Tissue 50 mg Kidney Tissue 100 mg Brain Tissue 250 mg Lung Tissue 100 mg Whole Blood 2 – 10 mL* Bacteria 2.5 x 10 10 cells Yeast 2 x 108 cells Fungi 1 g Plant Tissues 1 g Time to Complete 4 Purifications 40 minutes Average Yield: HeLa Cells (5 x 107 cells) E. coli (2.5 x 1010 cells) ~750 μg ~1.5 mg *For isolating total RNA from purified leukocytes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Component Cat. 26800 (8 preps) Buffer RL 2 x 40 mL Wash Solution A 4 x 38 mL Elution Solution A 3 x 20 mL RNA Maxi Spin Columns (with collection tubes) 8 Elution Tubes (50 mL) 8 Product Insert 1 Documentation PROTOCOLS (26800) Total RNA Purification Maxi Kit - Protocol (8 prep) SAFETY DATA SHEETS (26800) Total RNA Purification Maxi Kit - Safety Data Sheet POSTERS FLYERS Total RNA Purification RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Powered by Bioz See more details on Bioz Related Products RNase-Free DNase I Kit Animal Tissue RNA Purification Kit Plant/Fungi Total RNA Purification Kit Cytoplasmic & Nuclear RNA Purification Kit Total RNA Purification Kit Total RNA Purification Plus Kit Total RNA Purification Micro Kit
Total RNA Purification Maxi Kit For the rapid purification of total RNA - including microRNA - without the use of phenol For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Total RNA Purification Maxi Kit For the rapid purification of total RNA - including microRNA - without the use of phenol Price USD$175.00 SKU 26800 Format Maxi Size 8 Preps Quantity