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Total RNA Purification Maxi Kit

Cat. 26800
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Total RNA Purification Maxi Kit

Cat. 26800

For the rapid purification of total RNA - including microRNA - without the use of phenol

  • Extract high quality & quantity total RNA including miRNA
  • No phenol step required - isolate all RNA in one fraction
  • Rapid processing in under 40 minutes
  • Isolate total RNA from a wide variety of specimens
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Total RNA Purification Maxi Kit
(Cat. 26800)
8 preps

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. 26800
Kit Specifications
Maximum Column Binding Capacity
1.5 mg
Maximum Column Loading Volume
20 mL
Size of RNA Purified
All sizes, including small RNA
(< 200 nt)
Maximum Amount of Starting Material
Liver Tissue
100 mg
Heart Tissue
50 mg
Kidney Tissue
100 mg
Brain Tissue
250 mg
Lung Tissue
100 mg
Whole Blood
2 – 10 mL*
Bacteria 2.5 x 10 10 cells
Yeast
2 x 108 cells
Fungi
1 g
Plant Tissues
1 g
Time to Complete 4 Purifications
40 minutes
Average Yield:
HeLa Cells (5 x 107 cells)
E. coli (2.5 x 1010 cells)
~750 μg
~1.5 mg

*For isolating total RNA from purified leukocytes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Click for expanded view

Total RNA Purification Maxi Kit

Norgen’s Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants, and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen’s Purification Technology

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). Ethanol is then added to the lysate, and the solution is loaded onto a maxi spin column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Solution A. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 26800
Kit Specifications
Maximum Column Binding Capacity
1.5 mg
Maximum Column Loading Volume
20 mL
Size of RNA Purified
All sizes, including small RNA
(< 200 nt)
Maximum Amount of Starting Material
Liver Tissue
100 mg
Heart Tissue
50 mg
Kidney Tissue
100 mg
Brain Tissue
250 mg
Lung Tissue
100 mg
Whole Blood
2 – 10 mL*
Bacteria 2.5 x 10 10 cells
Yeast
2 x 108 cells
Fungi
1 g
Plant Tissues
1 g
Time to Complete 4 Purifications
40 minutes
Average Yield:
HeLa Cells (5 x 107 cells)
E. coli (2.5 x 1010 cells)
~750 μg
~1.5 mg

*For isolating total RNA from purified leukocytes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

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