Total RNA Purification Maxi Kit (Cat. 26800)

Extract high quality & purity total RNA including miRNA
No phenol step required - isolate all RNA in one fraction
Rapid processing in under 40 minutes
Isolate total RNA from a wide variety of specimens

Items	Cat. #	Size
Total RNA Purification Maxi Kit	26800	8 preps

Total RNA Purification Maxi Kit

This kit provides a rapid method for the isolation and purification of total RNA from large inputs of various biological specimens, including cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses. The kit purifies all sizes of RNA including microRNA (miRNA).

The extracted RNA is of a high quality and purity with excellent RIN values and A260/A280 ratios, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, expression profiling, miRNA profiling, microarrays and Next Generation Sequencing.

Kit Specifications
Column Binding Capacity	1.5 mg
Maximum Column Loading Volume	20 mL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum amount of starting material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues	5 x 10⁷ cells 50 - 250 mg 2 – 10 mL* 2.5 x 10¹⁰ cells 2 x 10⁸ cells 1 g 1 g
Time to Complete 4 Purifications	40 minutes
Average Yields HeLa Cells (5 x 10^{7 cells}) E. coli (2.5 x 10¹⁰ cells)	750 μg 1.5 mg

*For isolating total RNA from purified leukocytes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Kit Components

Component	Cat. 26800 (8 preps)
Buffer RL	2 x 40 mL
Wash Solution A	4 x 38 mL
Elution Solution A	3 x 20 mL
RNA Maxi Spin Columns (with collection tubes)	8
Elution Tubes (50 mL)	8
Product Insert	1