Total RNA Purification Plus Kit For the purification of total RNA - including microRNA - with rapid gDNA removal For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Total RNA Purification Plus Kit For the purification of total RNA - including microRNA - with rapid gDNA removal Price USD$399.00 SKU 48300 Format Plus Size 50 Preps 100 Preps Quantity Overview Details Documentation Citations Overview Extract high quality & quantity total RNA including miRNA No phenol step required; isolate all RNA in one fraction Genomic DNA Removal Column for efficient elimination of gDNA Bind & elute all RNA irrespective of size or GC content, without bias Efficiently extract small RNA irrespective of GC content Very sensitive & linear down to a few cells without the need for carrier RNA Convenient & fast spin column format Isolate from a wide variety of specimens Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step. Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content. Details Supporting Data Previous Figure 1. Genomic DNA Removal from HeLa cells. Norgen's Total RNA Purification Plus Kit is unique in that it contains a Genomic DNA Removal Column, allowing for gDNA removal without the use of enzymes. HeLa cells were either passed through the gDNA Removal Column, or received no gDNA removal treatment. The Genomic DNA Removal Column successfully removed gDNA from both 1 million HeLa cells (Panel A) and 1x105 HeLa cells (Panel B). For both Panels A and B, the left image displays the RNA run on a 1.2% EtBR-agarose gel, to better visualize any gDNA contamination. The right image displays a 1.0% formaldehyde-agarose gel to allow for better denaturation of RNA in the sample. For higher inputs, as in image A, the Genomic DNA Removal Column also resulted in higher RNA yields than the non-treated samples. Figure 2. Delta Ct Values Observed Between Different gDNA Removal Techniques. Genomic DNA is well known to contaminate RNA samples, and subsequently RT-qPCR results. Therefore, to determine the amount of genomic DNA contamination in RNA samples, an RT-qPCR can be used to compare Ct values observed with and without reverse transcription of the RNA sample. This is known as the Delta Ct. One million and 1x105 HeLa cells were either passed through the gDNA Removal Column or subjected to DNase-treatment using Norgen's RNase-Free DNase I (Cat# 25710). The gDNA Removal column eliminated gDNA similar to a DNase treatment. For higher inputs (i.e. 1 Million HeLa cells), the gDNA Removal Column successfully removed more gDNA contamination from the RNA samples than the DNase-treatment. When inputs were lowered, the amount of genomic DNA contamination in the DNase-treated sample was similar to the sample passed through the gDNA Removal Column. Both methods work well for removing gDNA contamination in RNA samples. Next Click for expanded view Kit Specifications Maximum Binding Capacity Per Column Up to 50 μg RNA Maximum Loading Volume Per Spin Column 650 μL Size of RNA Purified All sizes, including < 200 nt Time to Complete 10 Purifications 25 minutes Maximum Amount of Starting Material Liver Tissue 20 mg Heart Tissue 5 mg Kidney Tissue 20 mg Brain Tissue 25 mg Lung Tissue 20 mg Spleen Tissue 20 mg Whole Blood 100 µL Hela Cells 3 x 106 CHO 3 x 106 Yeast 1 x 108 cells E.coli 1 x 109 CFU/mL Bacteria 1 x 109 cells Plasma/Serum 200 µL Fungi 50 mg Plant Tissue 50 mg Viruses ≤100 µL of viral suspension Average Yield HeLa Cells (1 x 106 cells) E. coli (1 x 109 cells) 15 µg 50 µg Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. ComponentCat. 48300 (50 preps)Cat. 48400 (100 preps)Buffer RL40 mL2 x 40 mLWash Solution A38 mL2 x 38 mLElution Solution A6 mL2 x 6 mLRNA Purification Columns50100Genomic DNA Removal Columns50100Collection Tubes100200Elution Tubes (1.7 mL)50100Product Insert11 Documentation PROTOCOLS (48300) Total RNA Purification Plus Kit - Protocol (50 prep) (48400) Total RNA Purification Plus Kit - Protocol (100 prep) SAFETY DATA SHEETS (48300, 48400) Total RNA Purification Plus Kit - Safety Data Sheet FLYERS Total RNA Purification Plus Kit Total RNA Purification RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Powered by Bioz See more details on Bioz Related Products Animal Tissue RNA Purification Kit Plant/Fungi Total RNA Purification Kit RNase-Free DNase I Kit Total RNA Purification Micro Kit Total RNA Purification Plus Micro Kit
Total RNA Purification Plus Kit For the purification of total RNA - including microRNA - with rapid gDNA removal For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Total RNA Purification Plus Kit For the purification of total RNA - including microRNA - with rapid gDNA removal Price USD$399.00 SKU 48300 Format Plus Size 50 Preps 100 Preps Quantity
