Total RNA Purification Plus Micro Kit Rapid purification of total RNA - including microRNA - from small input amounts For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Total RNA Purification Plus Micro Kit Rapid purification of total RNA - including microRNA - from small input amounts Price USD$439.00 SKU 48500 Format Micro Plus Size 50 Preps Quantity Overview Details Documentation Citations Overview Extract high quality & quantity total RNA including miRNA Elute extracted total RNA in smaller volume (20 µL) No phenol step required - isolate all RNA in one fraction Genomic DNA Removal Column for efficient elimination of gDNA Bind & elute all RNA irrespective of size or GC content, without bias. Efficiently extract small RNA irrespective of GC content Very sensitive & linear down to a few cells without the need for carrier RNA Convenient & fast spin column format Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s Total RNA Purification Plus Micro Kit provides a rapid and sensitive method for the isolation and purification of total RNA from small input amounts of cultured animal cells, tissue samples, and microdissected samples including laser-capture microdissection (LCM). The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). Genomic DNA is removed from the sample using a Genomic DNA Removal column, and the RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. Norgen’s Purification Technology Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). The DNA is then captured on a Genomic DNA Removal Column. Ethanol is then added to the flowthrough of the DNA removal step, and the solution is loaded onto an RNA Purification Micro Column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Solution A. The special design of the micro spin-column allows a small elution volume of as little as 20 μL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications. Details Supporting Data Previous Figure 1. Great Isolation Sensitivity. Norgen's Total RNA Purification Plus Micro Kit allows sensitive RNA extraction from as little as a single cell. Total RNA was extracted from a decreasing number of HeLa cells followed by RT-qPCR to detect the human S14 transcript in the isolated RNA. PCR product of S14 was detected from as little as a single cell. Next Click for expanded view Kit Specifications Maximum Column Binding Capacity 35 μg Maximum Column Loading Volume 650 μL Minimum Elution Volume 20 μL Size of RNA Purified All sizes, including small RNA (< 200 nt) Maximum Amount of Starting Material: Animal Cells Animal Tissues (Brain, Kidney, Liver, Lung, Spleen) Heart, Muscle Laser-Captured Microdissection (LCM) 5 x 105 cells 3 mg (for most tissues*) Up to 7.5 mL Up to 5 x 105 cells Time to Complete 10 Purifications 22 minutes Average Yield* HeLa Cells (1 x 105 cells) 1.5 μg * for fibrous tissue, an additional Proteinase K treatment is required Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. Component Cat. 48500 (50 preps) Buffer RL 40 mL Wash Solution A 38 mL Elution Solution A 6 mL Genomic DNA Removal Column 50 RNA Purification Micro Column 50 Collection Tubes 100 Elution Tubes (1.7 mL) 50 Product Insert 1 Documentation PROTOCOLS (48500) Total RNA Purification Plus Micro Kit - Protocol (50 prep) APPLICATION NOTES Total RNA/DNA Purification and Detection from Blood Preserved on a Neoteryx™ Mitra Microsampling Device SAFETY DATA SHEETS (48500) Total RNA Purification Plus Micro Kit - Safety Data Sheet FLYERS Total RNA Purification Plus Kit RELATED BLOGS 3 Reasons why Norgen’s Industry-Leading Patented RNA Purification Technology is Best-in-Class How to Reduce False Negative SARS-CoV-2 Results with Silicon Carbide Citations Powered by Bioz See more details on Bioz Related Products RNase-Free DNase I Kit Animal Tissue RNA Purification Kit Plant/Fungi Total RNA Purification Kit Cytoplasmic & Nuclear RNA Purification Kit Total RNA Purification Kit Total RNA Purification Plus Kit Total RNA Purification Micro Kit Total RNA Purification Maxi Kit
Total RNA Purification Plus Micro Kit Rapid purification of total RNA - including microRNA - from small input amounts For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Total RNA Purification Plus Micro Kit Rapid purification of total RNA - including microRNA - from small input amounts Price USD$439.00 SKU 48500 Format Micro Plus Size 50 Preps Quantity
