Urine Cell-Free Circulating and Viral Nucleic Acid Purification Kits

Cat. 59900, 60000, 60100
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Urine Cell-Free Circulating and Viral Nucleic Acid Purification Kits

Cat. 59900, 60000, 60100

For isolating total circulating nucleic acids (DNA and RNA) from various urine inputs ranges

  • Isolate all sizes of circulating DNA, circulating and exosomal RNA, including microRNA, viral DNA/RNA in one elution
  • Versatile urine input ranges
  • No phenol extractions nor carrier RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Concentrate circulating DNA, circulating RNA and exosomal RNA, viral DNA, viral RNA into a flexible elution volumes
  • Compatible with fresh, frozen or preserved urine sample
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Urine Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit
(Cat. 59900)
50 preps
Urine Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit
(Cat. 60000)
20 preps
Urine Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit
(Cat. 60100)
10 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Isolate all sizes of circulating DNA, circulating and exosomal RNA, including microRNA, viral DNA/RNA in one elution
  • Versatile urine input ranges
  • No phenol extractions nor carrier RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Concentrate circulating DNA, circulating RNA and exosomal RNA, viral DNA, viral RNA into a flexible elution volumes
  • Compatible with fresh, frozen or preserved urine sample
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Related Services

Urine Cell-Free Circulating and Viral Nucleic Acid Purification Kits

These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA, circulating RNA including exosomal RNA as well as viral DNA/RNA from fresh, frozen or preserved urine samples. Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix. These kits are designed to isolate all sizes of cfc-DNA and cfc-RNA, including microRNA, as well as all sizes of exosomal RNA. Norgen’s Urine Cell-Free Circulating and Viral Nucleic Acid Purification Kits provide a clear advantage over other available kits in that they do not require phenol/chloroform or any protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified nucleic Acid is of the highest integrity, and can be used in a any downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, methylation-sensitive PCR and Southern Blot analysis, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Background

Recent evidence indicates that cell-free circulating (cfc) nucleic acids such as DNA and RNA, including exosomal RNA, in urine contains valuable information for the discovery of biomarkers that can help with early detection of certain cancer types, for monitoring the disease status as well as for the detection fetal DNA. Moreover, urine has been widely used for the detection of viral infections, including HIV, HCV and HBV.The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures, and repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acids for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acids is similar to that in urine or serum; (3) nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood).

Urine Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit

For sample input volumes ranging from 250 µL to 2 mL.

Urine Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit

For sample input volumes ranging from 2 mL - 10 mL.

Urine Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit

For sample input volumes ranging from 10 mL - 30 mL.

Kit Specifications

You have selected: Cat. 59900
Kit Specifications
Minimum Urine Input
250 μL
Maximum Urine Input
2 mL
Size of Nucleic Acid Purified
All sizes, including miRNA and
small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
25-30 minutes
Average Yields
Variable depending on specimen

 

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature. Theses kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 60000
Kit Specifications
Minimum Urine Input
2 mL
Maximum Urine Input
10 mL
Size of Nucleic Acid Purified
All sizes, including miRNA and
small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen

 

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature. Theses kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 60100
Kit Specifications
Minimum Urine Input
10 mL
Maximum Urine Input
30 mL
Size of Nucleic Acid Purified
All sizes, including miRNA and
small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen

 

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 year without showing any reduction in performance. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Supporting Data

Click for expanded view

Component Cat. 59900 (50 preps) Cat. 60000 (20 preps) Cat. 60100 (10 preps)
Binding Solution K 25 mL 75 mL 1 x 75 mL
1 x 25 mL
Lysis Buffer A 30 mL 20 mL 20 mL
Wash Solution A 18 mL 18 mL 18 mL
Elution Buffer F 6 mL 6 mL 6 mL
Mini Spin Columns 50 20 10
Midi Spin Columns - 20 -
Maxi Spin Columns - - 10
Collection Tubes 50 20 10
Elution Tubes (1.7 mL) 50 20 10
Product Insert 1 1 1

Urine Cell-Free Circulating and Viral Nucleic Acid Purification Kits

These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA, circulating RNA including exosomal RNA as well as viral DNA/RNA from fresh, frozen or preserved urine samples. Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix. These kits are designed to isolate all sizes of cfc-DNA and cfc-RNA, including microRNA, as well as all sizes of exosomal RNA. Norgen’s Urine Cell-Free Circulating and Viral Nucleic Acid Purification Kits provide a clear advantage over other available kits in that they do not require phenol/chloroform or any protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified nucleic Acid is of the highest integrity, and can be used in a any downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, methylation-sensitive PCR and Southern Blot analysis, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Background

Recent evidence indicates that cell-free circulating (cfc) nucleic acids such as DNA and RNA, including exosomal RNA, in urine contains valuable information for the discovery of biomarkers that can help with early detection of certain cancer types, for monitoring the disease status as well as for the detection fetal DNA. Moreover, urine has been widely used for the detection of viral infections, including HIV, HCV and HBV.The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures, and repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acids for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acids is similar to that in urine or serum; (3) nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood).

Urine Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit

For sample input volumes ranging from 250 µL to 2 mL.

Urine Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit

For sample input volumes ranging from 2 mL - 10 mL.

Urine Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit

For sample input volumes ranging from 10 mL - 30 mL.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 59900
Kit Specifications
Minimum Urine Input
250 μL
Maximum Urine Input
2 mL
Size of Nucleic Acid Purified
All sizes, including miRNA and
small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
25-30 minutes
Average Yields
Variable depending on specimen

 

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature. Theses kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 60000
Kit Specifications
Minimum Urine Input
2 mL
Maximum Urine Input
10 mL
Size of Nucleic Acid Purified
All sizes, including miRNA and
small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen

 

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature. Theses kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 60100
Kit Specifications
Minimum Urine Input
10 mL
Maximum Urine Input
30 mL
Size of Nucleic Acid Purified
All sizes, including miRNA and
small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen

 

Storage Conditions and Product Stability

All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 2 year without showing any reduction in performance. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Component Cat. 59900 (50 preps) Cat. 60000 (20 preps) Cat. 60100 (10 preps)
Binding Solution K 25 mL 75 mL 1 x 75 mL
1 x 25 mL
Lysis Buffer A 30 mL 20 mL 20 mL
Wash Solution A 18 mL 18 mL 18 mL
Elution Buffer F 6 mL 6 mL 6 mL
Mini Spin Columns 50 20 10
Midi Spin Columns - 20 -
Maxi Spin Columns - - 10
Collection Tubes 50 20 10
Elution Tubes (1.7 mL) 50 20 10
Product Insert 1 1 1
 


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