Urine Cell-Free Circulating RNA Purification Kits

Cat. 56900, 57000, 57100

Urine Cell-Free Circulating RNA Purification Mini Kit

Urine Cell-Free Circulating RNA Purification Midi Kit

Urine Cell-Free Circulating RNA Purification Maxi Kit

Urine Cell-Free Circulating RNA Purification Mini Kit Label

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Urine Cell-Free Circulating RNA Purification Kits

Cat. 56900, 57000, 57100

For isolating circulating RNA and exosomal RNA from urine

Isolate all sizes of circulating and exosomal RNA, including microRNA
Versatile urine input ranges
No phenol extractions
No carrier RNA
Bind and elute all RNA irrespective of size or GC content, without bias
Concentrate circulating RNA and exosomal RNA into flexible elution volumes
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Urine Cell-Free Circulating RNA Purification Mini Kit
(Cat. 56900) 50 preps

Urine Cell-Free Circulating RNA Purification Midi Kit
(Cat. 57000) 20 preps

Urine Cell-Free Circulating RNA Purification Maxi Kit
(Cat. 57100) 10 preps

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Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Kit Specifications

You have selected: Cat. 56900

Kit Specifications
Sample Volume Range	250 μL to 2 mL
Size of RNA Purified	All sizes including small RNA (< 200 nt)
Minimum Elution Volume	50 μL
Maximum Elution Volume	100 μL
Time to Complete 10 Purifications	25-30 minutes
Average Yield*	Variable depending on specimen

*Please check page 6 of the product insert for average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 57000

Kit Specifications
Minimum Urine Input	2 mL
Maximum Urine Input	10 mL
Size of RNA Purified	All sizes including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	40-45 minutes
Average Yields	Variable depending on specimen

*Please check page 6 of the product insert for average yields and common RNA quantification methods.

You have selected: Cat. 57100

Kit Specifications
Minimum Urine Input	10 mL
Maximum Urine Input	30 mL
Size of RNA Purified	All sizes including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	40-45 minutes
Average Yields	Variable depending on specimen

*Please check page 6 of the product insert for average yields and common RNA quantification methods.

Supporting Data

Figure 1. Purification of cell-free circulating RNA and exosomal RNA from different urine volumes. Norgen's Urine Cell-Free Circulating RNA Purification Mini Kit (Cat# 56900) was used to purify cell-free circulating and exosomal RNA from 0.5 mL, 1 mL and 2 mL urine samples. Two microlilitres of the purified RNA was then used as the template in RT-qPCR reactions to assess the amplification of (A) the housekeeping 5S rRNA transcript and (B) miR-21. The average Ct value for both (A) 5S rRNA transcript and (B) miR-21 is linearly decreasing with increasing the sample input volume.

Figure 2. Linearity of RNA purified from increasing urine volumes. Norgen's Urine Cell-Free Circulating RNA Purification Mini Kit (Cat# 56900) was used to purify RNA from 0.5 mL, 1 mL and 2 mL urine samples. Two microlitres of the purified RNA was then used as the template in RT-qPCR reactions to assess the linearity of the (A) 5S rRNA transcript and (B) miR-21 from the different urine volumes. Norgen's Urine Cell-Free Circulating RNA Purification Mini Kit was able to recover 96% of the 5S rRNA transcript from 1 mL urine relative to the amount that is present in 0.5 mL plasma. Moreover, 96% of the 5S rRNA transcript was recovered from 2 mL urine relative to the amount that is present in 1 mL urine. As for miR-21, Norgen's Urine Cell-Free Circulating RNA Purification Mini Kit was able to recover 92% of miR-21 from 1 mL urine relative to the amount that is present in 0.5 mL urine. Furthermore, 100% of miR-21 was recovered from 2 mL urine relative to the amount that is present in 1 mL urine.

Figure 3. Determination of the amount of inhibition present in urine cell-free circulating RNA samples when detecting the human miR-21.RNA was isolated from 0.5 mL, 1 mL and 2 mL urine using Norgen's Urine Cell-Free Circulating RNA Purification Mini Kit (Cat# 56900). Increasing volumes of the elution (2, 4 and 8 L) were used in a 20 L qPCR reaction to observe any decrease in Ct value. An increase in Ct values with increasing amount of template would be a clear indication of PCR inhibitors present in the sample. An increase in elution volume used as a template in the qPCR did not affect the Ct value generated from qPCR and in fact the Ct values tend to decrease with increasing the PCR input volume, indicating that RNA purified from urine using Norgen's kit is free of the common inhibitors usually present in urine.

Figure 4. Purification of cell-free circulating RNA and exosomal RNA from different urine volumes. Norgen's Urine Cell-Free Circulating RNA Purification Midi Kit (Cat# 57000) was used to purify cell-free circulating and exosomal RNA from 2 mL, 5 mL and 10 mL urine samples. Two microlilitres of the purified RNA was then used as the template in RT-qPCR reactions to assess the amplification of (A) the housekeeping 5S rRNA transcript and (B) miR-21. The average Ct value for both (A) 5S rRNA transcript and (B) miR-21 is linearly decreasing with increasing the sample input volume.

Figure 5. Linearity of RNA purified from increasing urine volumes. Norgen's Urine Cell-Free Circulating RNA Purification Midi Kit (Cat# 57000) was used to purify RNA from 2 mL, 5 mL and 10 mL urine samples. Two microlitres of the purified RNA was then used as the template in RT-qPCR reactions to assess the linearity of the (A) 5S rRNA transcript and (B) miR-21 from the different urine volumes. Norgen's Urine Cell-Free Circulating RNA Purification Midi Kit was able to recover 96% of the 5S rRNA transcript from 5 mL urine relative to the amount that is present in 2 mL plasma. Moreover, 97% of the 5S rRNA transcript was recovered from 10 mL urine relative to the amount that is present in 5 mL urine. As for miR-21, Norgen's Urine Cell-Free Circulating RNA Purification Midi Kit was able to recover 98% of miR-21 from 5 mL urine relative to the amount that is present in 1 mL urine. Furthermore, 92% of miR-21 was recovered from 10 mL urine relative to the amount that is present in 5 mL urine.

Figure 6. Determination of the amount of inhibition present in urine cell-free circulating RNA samples when detecting the human miR-21. RNA was isolated from 2 mL, 5 mL and 10 mL urine using Norgen's Urine Cell-Free Circulating RNA Purification Midi Kit (Cat# 57000). Increasing volumes of the elution (2, 4 and 8 L) were used in a 20 L qPCR reaction to observe any decrease in Ct value. An increase in Ct values with increasing amount of template would be a clear indication of PCR inhibitors present in the sample. An increase in elution volume used as a template in the qPCR did not affect the Ct value generated from qPCR and in fact the Ct values tend to decrease with increasing the PCR input volume indicating that RNA purified from urine using Norgen's kit is free of the common inhibitors usually present in urine.

Figure 7. Purification of cell-free Circulating RNA and exosomal RNA from different urine volumes. Norgens Urine Cell-Free Circulating RNA Purification Maxi Kit (Cat# 57100) was used to purify cell-free circulating and exosomal RNA from 10 mL, 20 mL and 30 mL urine samples. Two microlilitres of the purified RNA was then used as the template in RT-qPCR reactions to assess the amplification of (A) the housekeeping 5S rRNA transcript and (B) miR-21. The average Ct value for both (A) 5S rRNA transcript and (B) miR-21 is linearly decreasing with increasing the sample input volume.

Figure 8. Linearity of RNA purified from increasing urine. Norgens Urine Cell-Free Circulating RNA Purification Maxi Kit (Cat# 57100) was used to purify RNA from 10 mL, 20 mL and 30 mL urine samples. Two microlitres of the purified RNA was then used as the template in RT-qPCR reactions to assess the linearity of the(A) 5S rRNA transcript and (B) miR-21 from the different urine volumes. Norgens Urine Cell-Free Circulating RNA Purification Maxi Kit was able to recover 95% of the 5S rRNA transcript from 20 mL urine relative to the amount that is present in 10 mL Urine. Moreover, 99% of the 5S rRNA transcript was recovered from 30 mL urine relative to the amount that is present in 20 mL urine. As for miR-21, Norgens Urine Cell-Free Circulating RNA Purification Maxi Kit was able to recover 99% of miR-21 from 20 mL urine relative to the amount that is present in 10 mL urine. Furthermore, 96% of miR-21 was recovered from 30 mL urine relative to the amount that is present in 20 mL urine.

Figure 9. Determination of the amount of inhibition present in urine cell-free circulating RNA samples when detecting the human miR-21. RNA was isolated from 10 mL, 20 mL and 30 mL urine using Norgens Urine Cell-Free Circulating RNA Purification Maxi Kit (Cat# 57100). Increasing volumes of the elution (2, 4 and 8 L) were used in a 20 L qPCR reaction to observe any decrease in Ct value. An increase in Ct values with increasing amount of template would be a clear indication of PCR inhibitors present in the sample. An increase in elution volume used as a template in the qPCR did not affect the Ct value generated from qPCR and in fact the Ct values tend to decrease with increasing the PCR input volume, indicating that RNA purified from urine using Norgens kit is free of the common inhibitors usually present in urine.

Click for expanded view

Protocols:

(56900) Urine Cell-Free Circulating RNA Purification Kits - Protocol (50 Preps)

(57000) Urine Cell-Free Circulating RNA Purification Kits - Protocol (20 Preps)

(57100) Urine Cell-Free Circulating RNA Purification Kits - Protocol (10 Preps)

Safety Data Sheets:

(56900, 57000, 57100) Urine Cell-Free Circulating RNA Purification Kits - Safety Data Sheet

Product Overview
Documentation

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Urine Cell-Free Circulating RNA Purification Kits

These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating RNA, including exosomal RNA as well as viral RNA from fresh, preserved or frozen urine samples. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. The purified urine RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Background

Recent evidence indicates that cell-free circulating RNA (cf-RNA) including exosomal RNA in urine contains valuable information for the discovery of biomarkers that can help for the early detection of certain cancer types and for monitoring the disease status as well as for the detection of any infectious pathogens. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures. In addition, repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acid for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acid is similar to that in plasma or serum but with a lower concentration; (3) Nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood).

Urine Cell-Free Circulating RNA Purification Mini Kit

For sample volumes ranging from 250 µL to 2 mL.

Urine Cell-Free Circulating RNA Purification Midi Kit

For sample volumes ranging from 2 mL to 10 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.

Urine Cell-Free Circulating RNA Purification Maxi Kit

For sample volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 56900

Kit Specifications
Sample Volume Range	250 μL to 2 mL
Size of RNA Purified	All sizes including small RNA (< 200 nt)
Minimum Elution Volume	50 μL
Maximum Elution Volume	100 μL
Time to Complete 10 Purifications	25-30 minutes
Average Yield*	Variable depending on specimen

*Please check page 6 of the product insert for average yields and the common RNA quantification methods.

You have selected: Cat. 57000

Kit Specifications
Minimum Urine Input	2 mL
Maximum Urine Input	10 mL
Size of RNA Purified	All sizes including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	40-45 minutes
Average Yields	Variable depending on specimen

*Please check page 6 of the product insert for average yields and common RNA quantification methods.

You have selected: Cat. 57100

Kit Specifications
Minimum Urine Input	10 mL
Maximum Urine Input	30 mL
Size of RNA Purified	All sizes including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	40-45 minutes
Average Yields	Variable depending on specimen

*Please check page 6 of the product insert for average yields and common RNA quantification methods.

Protocols:

(56900) Urine Cell-Free Circulating RNA Purification Kits - Protocol (50 Preps)

(57000) Urine Cell-Free Circulating RNA Purification Kits - Protocol (20 Preps)

(57100) Urine Cell-Free Circulating RNA Purification Kits - Protocol (10 Preps)

Safety Data Sheets:

(56900, 57000, 57100) Urine Cell-Free Circulating RNA Purification Kits - Safety Data Sheet

Improve Your Workflow with Best-in-Class Total RNA Purification Technology | Learn More

Urine Cell-Free Circulating RNA Purification Kits (Cat. 56900, 57000, 57100)

Urine Cell-Free Circulating RNA Purification Kits

Urine Cell-Free Circulating RNA Purification Kits

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Related Services

NGS

RNA Isolation

Related Products

Urine Cell-Free Circulating RNA Purification Kits (Cat. 56900, 57000, 57100)

Receive ComplimentaryNUCLEASE-FREE WATER

Urine Cell-Free Circulating RNA Purification Kits

Urine Cell-Free Circulating RNA Purification Kits

Product Format Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Product Size

Product Overview

Documentation

Related Services

NGS

RNA Isolation

Related Products

microRNA (cel-miR-39) Spike-In Kit

Urine Cell-Free Circulating DNA Purification Kits

Receive Complimentary
NUCLEASE-FREE WATER

Product Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.