Urine Cell-Free Circulating RNA Purification Kits

Cat. 56900, 57000, 57100
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Urine Cell-Free Circulating RNA Purification Kits

Cat. 56900, 57000, 57100

For isolating circulating RNA and exosomal RNA from urine

  • Isolate all sizes of circulating and exosomal RNA, including microRNA
  • Versatile urine input ranges
  • No phenol extractions
  • No carrier RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Concentrate circulating RNA and exosomal RNA into flexible elution volumes
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

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Urine Cell-Free Circulating RNA Purification Mini Kit
(Cat. 56900)
50 preps
Urine Cell-Free Circulating RNA Purification Midi Kit
(Cat. 57000)
20 preps
Urine Cell-Free Circulating RNA Purification Maxi Kit
(Cat. 57100)
10 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Isolate all sizes of circulating and exosomal RNA, including microRNA
  • Versatile urine input ranges
  • No phenol extractions
  • No carrier RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Concentrate circulating RNA and exosomal RNA into flexible elution volumes
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Related Services

Urine Cell-Free Circulating RNA Purification Kits

These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating RNA, including exosomal RNA as well as viral RNA from fresh, preserved or frozen urine samples. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. The purified urine RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Background

Recent evidence indicates that cell-free circulating RNA (cf-RNA) including exosomal RNA in urine contains valuable information for the discovery of biomarkers that can help for the early detection of certain cancer types and for monitoring the disease status as well as for the detection of any infectious pathogens. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures. In addition, repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acid for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acid is similar to that in plasma or serum but with a lower concentration; (3) Nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood).

Urine Cell-Free Circulating RNA Purification Mini Kit

For sample volumes ranging from 250 µL to 2 mL.

Urine Cell-Free Circulating RNA Purification Midi Kit

For sample volumes ranging from 2 mL to 10 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.

Urine Cell-Free Circulating RNA Purification Midi Kit

For sample volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL

Kit Specifications

You have selected: Cat. 56900
Kit Specifications
Sample Volume Range
250 μL to 2 mL
Size of RNA Purified
All sizes including small RNA (< 200 nt)
Minimum Elution Volume
50 μL
Maximum Elution Volume
100 μL
Time to Complete 10 Purifications
25-30 minutes
Average Yield*
Variable depending on specimen

*Please check page 6 of the product insert for average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 57000
Kit Specifications
Minimum Urine Input
2 mL
Maximum Urine Input
10 mL
Size of RNA Purified
All sizes including miRNA
and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen

*Please check page 6 of the product insert for average yields and common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 57100
Kit Specifications
Minimum Urine Input
10 mL
Maximum Urine Input
30 mL
Size of RNA Purified
All sizes including miRNA
and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen

*Please check page 6 of the product insert for average yields and common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Supporting Data

Click for expanded view

Component Cat. 56900 (50 preps) Cat. 57000 (20 preps) Cat. 57100 (10 preps)
Binding Solution K 25 mL 75 mL 1 x 75 mL
1 x 25 mL
Lysis Buffer A 30 mL 20 mL 20 mL
Wash Solution A 18 mL 18 mL 18 mL
Elution Solution A 6 mL 6 mL 6 mL
Mini Spin Columns 50 20 10
Midi Spin Columns - 20 -
Maxi Spin Columns - - 10
Collection Tubes 50 20 10
Elution Tubes (1.7 mL) 50 20 10
Product Insert 1 1 1

Urine Cell-Free Circulating RNA Purification Kits

These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating RNA, including exosomal RNA as well as viral RNA from fresh, preserved or frozen urine samples. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. The purified urine RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Background

Recent evidence indicates that cell-free circulating RNA (cf-RNA) including exosomal RNA in urine contains valuable information for the discovery of biomarkers that can help for the early detection of certain cancer types and for monitoring the disease status as well as for the detection of any infectious pathogens. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures. In addition, repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acid for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acid is similar to that in plasma or serum but with a lower concentration; (3) Nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood).

Urine Cell-Free Circulating RNA Purification Mini Kit

For sample volumes ranging from 250 µL to 2 mL.

Urine Cell-Free Circulating RNA Purification Midi Kit

For sample volumes ranging from 2 mL to 10 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.

Urine Cell-Free Circulating RNA Purification Midi Kit

For sample volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 56900
Kit Specifications
Sample Volume Range
250 μL to 2 mL
Size of RNA Purified
All sizes including small RNA (< 200 nt)
Minimum Elution Volume
50 μL
Maximum Elution Volume
100 μL
Time to Complete 10 Purifications
25-30 minutes
Average Yield*
Variable depending on specimen

*Please check page 6 of the product insert for average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 57000
Kit Specifications
Minimum Urine Input
2 mL
Maximum Urine Input
10 mL
Size of RNA Purified
All sizes including miRNA
and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen

*Please check page 6 of the product insert for average yields and common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

You have selected: Cat. 57100
Kit Specifications
Minimum Urine Input
10 mL
Maximum Urine Input
30 mL
Size of RNA Purified
All sizes including miRNA
and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen

*Please check page 6 of the product insert for average yields and common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Component Cat. 56900 (50 preps) Cat. 57000 (20 preps) Cat. 57100 (10 preps)
Binding Solution K 25 mL 75 mL 1 x 75 mL
1 x 25 mL
Lysis Buffer A 30 mL 20 mL 20 mL
Wash Solution A 18 mL 18 mL 18 mL
Elution Solution A 6 mL 6 mL 6 mL
Mini Spin Columns 50 20 10
Midi Spin Columns - 20 -
Maxi Spin Columns - - 10
Collection Tubes 50 20 10
Elution Tubes (1.7 mL) 50 20 10
Product Insert 1 1 1
 


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