Urine Exfoliated Cell RNA Purification Kit (Cat. 22550)

For the rapid purification of total RNA (including microRNA) from exfoliated cells in urine

  • Purify all sizes of RNA (including microRNA) without the need for phenol
  • Isolate and detect total RNA from 1 mL and up to 50 mL urine
  • Provides high quality RNA for sensitive applications - isolate RNA from as little as 100 cells
  • Rapid processing time from sampling to downstream testing

ItemsCat. #Size
Urine Exfoliated Cell RNA Purification Kit 22550 25 preps

Urine Exfoliated Cell RNA Purification Kit

This kit provides a rapid spin column method for the isolation and purification of total RNA (including microRNA) from exfoliated cells that have been shed into the urine from the urinary tract.  The total RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform.  The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).  The RNA is preferentially purified from other cellular components such as proteins, as well as from the contaminating species found in urine such as glucose and salts, without the use of phenol or chloroform.  The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, qRT-PCR, Northern blotting, NGS and expression array assays.  Multiple samples can be processed in 20 minutes.  ​

Background

RNA biomarkers from exfoliated cells can be used as non-invasive tools for a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers. 

Kit Specifications
Maximum Column Binding Capacity
50 μg
Volume of Urine Processed
1-50 mL
Maximum Input of Exfoliated Cells
1 x 106
Size of RNA Purified
All sizes, including small RNA
(< 200 nt)
Time to Complete 10 Purifications
20 minutes (for Exfoliated Cells)
30 minutes (for Bacteria)
Average Yield
~1μg RNA per 1 x 105 cells
(For Exfoliated Cells - Varies due to cell density sample)
~0.5μg RNA per 1 x 107 cells
(For Bacteria - Varies due to cell density sample)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  These reagents should remain stable for at least 1 year in their unopened containers.
 

 

Title Development of a Voided Urine Assay for Detecting Prostate Cancer Noninvasively: A Pilot Study
Journal BJU International. 2017.
Authors Trabulsi EJ, Tripathi SK, Gomella L, Solomides C, Wickstrom E, Thakur ML
Title Evaluation of urinary XIAP as a diagnostic biomarker of carcinoma of urinary bladder.
Journal Tumor Biology. 2014.
Authors Vaccaro M, Malafronte N, Alfieri M, Tommasi N, Leone A.
Title Long Noncoding RNAs as Putative Biomarkers for Prostate Cancer Detection.
Journal The Journal of Molecular Diagnostics. 2014.
Authors Bongyong Lee, Joseph Mazar, Muhammad N. Aftab, Feng Qi, John Shelley, Jian-Liang Li, Subramaniam Govindarajan, Felipe Valerio, Inoel Rivera, Tadzia Thurn, Tien Anh Tran, Darian Kameh, Vipul Patel‡, Ranjan J. Perera.
Title A pilot study of urinary microRNA as a biomarker for urothelial cancer.
Journal Canadian Urolological Association Journal. 2013.
Authors Snowdon J, Boag S, Feilotter H, Izard J, Siemens DR.