For the rapid purification of total RNA (including microRNA) from exfoliated cells in urine.
|Urine Exfoliated Cell RNA Purification Kit||22550||25 preps|
Urine Exfoliated Cell RNA Purification Kit
This kit provides a rapid spin column method for the isolation and purification of total RNA (including microRNA) from exfoliated cells that have been shed into the urine from the urinary tract. The total RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, as well as from the contaminating species found in urine such as glucose and salts, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, qRT-PCR, Northern blotting, NGS and expression array assays. Multiple samples can be processed in 20 minutes.
RNA biomarkers from exfoliated cells can be used as non-invasive tools for a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers.
|Column Binding Capacity||
|Volume of Urine Processed||
|Maximum Input of Exfoliated Cells||
1 x 106
|Size of RNA Purified||
All sizes, including small RNA
(< 200 nt)
|Time to Complete 10 Purifications||
~1μg RNA per 1 x 105 cells
(Varies due to cell density sample)
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
|Title||Evaluation of urinary XIAP as a diagnostic biomarker of carcinoma of urinary bladder.|
|Journal||Tumor Biology. 2014.|
|Authors||Vaccaro M, Malafronte N, Alfieri M, Tommasi N, Leone A.|
|Title||Long Noncoding RNAs as Putative Biomarkers for Prostate Cancer Detection.|
|Journal||The Journal of Molecular Diagnostics. 2014.|
|Authors||Bongyong Lee, Joseph Mazar, Muhammad N. Aftab, Feng Qi, John Shelley, Jian-Liang Li, Subramaniam Govindarajan, Felipe Valerio, Inoel Rivera, Tadzia Thurn, Tien Anh Tran, Darian Kameh, Vipul Patel‡, Ranjan J. Perera.|
|Title||A pilot study of urinary microRNA as a biomarker for urothelial cancer.|
|Journal||Canadian Urolological Association Journal. 2013.|
|Authors||Snowdon J, Boag S, Feilotter H, Izard J, Siemens DR.|
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