Urine Total RNA Purification Maxi Kit Dx (Slurry Format)

Cat. Dx29600
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Urine Total RNA Purification Maxi Kit Dx (Slurry Format)

Cat. Dx29600

Rapid purification of total RNA from 5 mL - 10 mL urine without phenol for in vitro diagnostic use

  • Isolate high quality total RNA and all sizes of circulating and exosomal RNA, including microRNA
  • No phenol extractions
  • Very sensitive and linear without the need for carrier RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Isolate inhibitor-free urinary RNA
  • Slurry/Spin column and Slurry/96-well format available
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Note: This product is not available for sale in the United States

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Urine Total RNA Purification Maxi Kit Dx (Slurry Format)
(Cat. Dx29600)
50 preps

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Isolate high quality total RNA and all sizes of circulating and exosomal RNA, including microRNA
  • No phenol extractions
  • Very sensitive and linear without the need for carrier RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Isolate inhibitor-free urinary RNA
  • Slurry/Spin column and Slurry/96-well format available
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Urine Total RNA Purification Maxi Kit Dx (Slurry Format)

Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) provides a fast, reliable and simple procedure for isolating total RNA from urine for subsequent in vitro diagnostic use. Purification is based on the use of Norgen’s proprietary resin as the separation matrix, and the kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs.

This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Urine Total RNA Purification Maxi Kit Dx (Slurry Format) in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.

To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.

Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques including RNA isolation.

Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

Kit Specifications

You have selected: Cat. Dx29600
Kit Specifications
Volume of Urine Processed
5 -  10 mL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 10 Purifications
< 30 minutes 
Average Yield
Variable depending on specimen


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Click for expanded view

Component Cat. Dx29600 (50 preps)
RNA Lysis Solution 3 x 90 mL
RNA Wash Solution 24 mL
RNA Elution Solution 6 mL
Mini Filter Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Total Extracellular Small RNA Profiles from Plasma, Saliva, and Urine of Healthy Subjects
Journal Scientific Reports. 2017.
Authors Yeri A, Courtright A, Reiman R, Carlson E, Beecroft T, Janss A, Siniard A, Richholt R, Balak C, Rozowsky J, Kitchen R, Hutchins E, Winarta J, McCoy R, Anastasi M, Kim S, Huentelman M, Van Keuren-Jensen K.
Title Supernatant versus exosomal urinary microRNAs. Two fractions with different outcomes in gynaecological cancers.
Journal Neoplasma. 2015.
Authors Zavesky L, Jandakova E, Turyna R, Langmeierova L, Weinberger V, Minar L
Title Evaluation of Cell-Free Urine microRNAs Expression for the Use in Diagnosis of Ovarian and Endometrial Cancers. A Pilot Study
Journal Pathology & Oncology Research. 2015.
Authors Luděk Záveský, Eva Jandáková, Radovan Turyna, Lucie Langmeierová, Vít Weinberger, Lenka Záveská Drábková, Martina Hůlková, Aleš Hořínek, Daniela Dušková, Jaroslav Feyereisl, Luboš Minář, Milada Kohoutová
Title Potential Urinary miRNA Biomarker Candidates for the Accurate Detection of Prostate Cancer among Benign Prostatic Hyperplasia Patients
Journal Journal of Cancer. 2014.
Authors Taha A Haj-Ahmad, Moemen AK Abdalla, Yousef Haj-Ahmad
Title Potential Urinary Protein Biomarker Candidates for the Accurate Detection of Prostate Cancer among Benign Prostatic Hyperplasia Patients
Journal Journal of Cancer. 2014.
Authors Taha A Haj-Ahmad, Moemen AK Abdalla, Yousef Haj-Ahmad

Urine Total RNA Purification Maxi Kit Dx (Slurry Format)

Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) provides a fast, reliable and simple procedure for isolating total RNA from urine for subsequent in vitro diagnostic use. Purification is based on the use of Norgen’s proprietary resin as the separation matrix, and the kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs.

This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Urine Total RNA Purification Maxi Kit Dx (Slurry Format) in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.

To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.

Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques including RNA isolation.

Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. Dx29600
Kit Specifications
Volume of Urine Processed
5 -  10 mL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 10 Purifications
< 30 minutes 
Average Yield
Variable depending on specimen


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component Cat. Dx29600 (50 preps)
RNA Lysis Solution 3 x 90 mL
RNA Wash Solution 24 mL
RNA Elution Solution 6 mL
Mini Filter Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1

Title Total Extracellular Small RNA Profiles from Plasma, Saliva, and Urine of Healthy Subjects
Journal Scientific Reports. 2017.
Authors Yeri A, Courtright A, Reiman R, Carlson E, Beecroft T, Janss A, Siniard A, Richholt R, Balak C, Rozowsky J, Kitchen R, Hutchins E, Winarta J, McCoy R, Anastasi M, Kim S, Huentelman M, Van Keuren-Jensen K.
Title Supernatant versus exosomal urinary microRNAs. Two fractions with different outcomes in gynaecological cancers.
Journal Neoplasma. 2015.
Authors Zavesky L, Jandakova E, Turyna R, Langmeierova L, Weinberger V, Minar L
Title Evaluation of Cell-Free Urine microRNAs Expression for the Use in Diagnosis of Ovarian and Endometrial Cancers. A Pilot Study
Journal Pathology & Oncology Research. 2015.
Authors Luděk Záveský, Eva Jandáková, Radovan Turyna, Lucie Langmeierová, Vít Weinberger, Lenka Záveská Drábková, Martina Hůlková, Aleš Hořínek, Daniela Dušková, Jaroslav Feyereisl, Luboš Minář, Milada Kohoutová
Title Potential Urinary miRNA Biomarker Candidates for the Accurate Detection of Prostate Cancer among Benign Prostatic Hyperplasia Patients
Journal Journal of Cancer. 2014.
Authors Taha A Haj-Ahmad, Moemen AK Abdalla, Yousef Haj-Ahmad
Title Potential Urinary Protein Biomarker Candidates for the Accurate Detection of Prostate Cancer among Benign Prostatic Hyperplasia Patients
Journal Journal of Cancer. 2014.
Authors Taha A Haj-Ahmad, Moemen AK Abdalla, Yousef Haj-Ahmad

 


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