Urine Total RNA Purification Maxi Kit (Slurry Format)

Cat. 29600, 29650
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Urine Total RNA Purification Maxi Kit (Slurry Format)

Cat. 29600, 29650

Rapid purification of total RNA from 5 mL - 10 mL urine without phenol

  • Isolate high quality total RNA and all sizes of circulating and exosomal RNA, including microRNA
  • No phenol extractions
  • Very sensitive and linear without the need for carrier RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Isolate inhibitor-free urinary RNA
  • Slurry/Spin column and Slurry/96-well format available
  • resin separation matrix -->
  • Purification is based on Norgen’s proprietary resin matrix

For research use only and NOT intended for in vitro diagnostics.

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Urine Total RNA Purification Maxi Kit (Slurry Format)
(Cat. 29600)
Variable
Urine Total RNA Purification Maxi 96-Well Kit (Slurry Format)
(Cat. 29650)
1 x 96-well plate

Kit Format
Norgen Biotek offers kits in different standardized formats. You can view and compare kit components in the Components Table in the Product Overview section. For more information on format types, please visit our Format Information page.

Kit Size

  • Isolate high quality total RNA and all sizes of circulating and exosomal RNA, including microRNA
  • No phenol extractions
  • Very sensitive and linear without the need for carrier RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Isolate inhibitor-free urinary RNA
  • Slurry/Spin column and Slurry/96-well format available
  • resin separation matrix -->
  • Purification is based on Norgen’s proprietary resin matrix

Urine Total RNA Purification Maxi Kit (Slurry Format)

Norgen’s Urine Total RNA Purification Maxi Kit (Slurry Format) provides a rapid method for the isolation and purification of total RNA from 5 – 10 mL of urine. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies showed that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease specific outcomes, and prediction of therapeutic responses.

Kit Specifications

You have selected: Cat. 29600
Kit Specifications
Volume of Urine Processed
5 - 10 mL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 10 Purifications
< 30 minutes
Average Yield
Variable depending on specimen


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 29650
Kit Specifications
Volume of Urine Processed
5 -  10 mL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 10 Purifications
< 30 minutes 
Average Yield
Variable depending on specimen


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Supporting Data

Click for expanded view

Component Cat. 29600 (variable) Cat. 29650 (96 preps)
Slurry C3 20 mL 2 x 24 mL
Lysis Buffer A 2 x 130 mL 4 x 130 mL
Wash Solution A 38 mL 2 x 20 mL
Elution Solution A 6 mL 2 x 6 mL
96-Well Filter Plate - 1
Adhesive Tape - 1
Mini Filter Spin Columns 50 -
Collection Tubes 50 -
96-Well Collection Plate - 1
Elution Tubes (1.7 mL) 50 -
96-Well Elution Plate - 1
Product Insert 1 1

Title Clinical Significance of Rab27a as a Urinary Biomarker in Patients With Bladder Cancer
Journal KJUO. 2022.
Authors Ja Yoon Ku, Eu Chang Hwang, Chan Ho Lee, Kyung Hwan Kim, Dong Deuk Kwon, Hong Koo Ha
Title Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes.
Journal Scientific Data. 2021.
Authors Hutchins, E., Reiman, R., Winarta, J. et al.
Title Total Extracellular Small RNA Profiles from Plasma, Saliva, and Urine of Healthy Subjects
Journal Scientific Reports. 2017.
Authors Yeri A, Courtright A, Reiman R, Carlson E, Beecroft T, Janss A, Siniard A, Richholt R, Balak C, Rozowsky J, Kitchen R, Hutchins E, Winarta J, McCoy R, Anastasi M, Kim S, Huentelman M, Van Keuren-Jensen K.
Title Supernatant versus exosomal urinary microRNAs. Two fractions with different outcomes in gynaecological cancers.
Journal Neoplasma. 2015.
Authors Zavesky L, Jandakova E, Turyna R, Langmeierova L, Weinberger V, Minar L
Title Evaluation of Cell-Free Urine microRNAs Expression for the Use in Diagnosis of Ovarian and Endometrial Cancers. A Pilot Study
Journal Pathology & Oncology Research. 2015.
Authors Luděk Záveský, Eva Jandáková, Radovan Turyna, Lucie Langmeierová, Vít Weinberger, Lenka Záveská Drábková, Martina Hůlková, Aleš Hořínek, Daniela Dušková, Jaroslav Feyereisl, Luboš Minář, Milada Kohoutová
Title Potential Urinary miRNA Biomarker Candidates for the Accurate Detection of Prostate Cancer among Benign Prostatic Hyperplasia Patients
Journal Journal of Cancer. 2014.
Authors Taha A Haj-Ahmad, Moemen AK Abdalla, Yousef Haj-Ahmad
Title Potential Urinary Protein Biomarker Candidates for the Accurate Detection of Prostate Cancer among Benign Prostatic Hyperplasia Patients
Journal Journal of Cancer. 2014.
Authors Taha A Haj-Ahmad, Moemen AK Abdalla, Yousef Haj-Ahmad

Urine Total RNA Purification Maxi Kit (Slurry Format)

Norgen’s Urine Total RNA Purification Maxi Kit (Slurry Format) provides a rapid method for the isolation and purification of total RNA from 5 – 10 mL of urine. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies showed that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease specific outcomes, and prediction of therapeutic responses.

Supporting Data

Click for expanded view

Kit Specifications

You have selected: Cat. 29600
Kit Specifications
Volume of Urine Processed
5 - 10 mL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 10 Purifications
< 30 minutes
Average Yield
Variable depending on specimen


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

You have selected: Cat. 29650
Kit Specifications
Volume of Urine Processed
5 -  10 mL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 10 Purifications
< 30 minutes 
Average Yield
Variable depending on specimen


Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component Cat. 29600 (variable) Cat. 29650 (96 preps)
Slurry C3 20 mL 2 x 24 mL
Lysis Buffer A 2 x 130 mL 4 x 130 mL
Wash Solution A 38 mL 2 x 20 mL
Elution Solution A 6 mL 2 x 6 mL
96-Well Filter Plate - 1
Adhesive Tape - 1
Mini Filter Spin Columns 50 -
Collection Tubes 50 -
96-Well Collection Plate - 1
Elution Tubes (1.7 mL) 50 -
96-Well Elution Plate - 1
Product Insert 1 1

Title Clinical Significance of Rab27a as a Urinary Biomarker in Patients With Bladder Cancer
Journal KJUO. 2022.
Authors Ja Yoon Ku, Eu Chang Hwang, Chan Ho Lee, Kyung Hwan Kim, Dong Deuk Kwon, Hong Koo Ha
Title Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes.
Journal Scientific Data. 2021.
Authors Hutchins, E., Reiman, R., Winarta, J. et al.
Title Total Extracellular Small RNA Profiles from Plasma, Saliva, and Urine of Healthy Subjects
Journal Scientific Reports. 2017.
Authors Yeri A, Courtright A, Reiman R, Carlson E, Beecroft T, Janss A, Siniard A, Richholt R, Balak C, Rozowsky J, Kitchen R, Hutchins E, Winarta J, McCoy R, Anastasi M, Kim S, Huentelman M, Van Keuren-Jensen K.
Title Supernatant versus exosomal urinary microRNAs. Two fractions with different outcomes in gynaecological cancers.
Journal Neoplasma. 2015.
Authors Zavesky L, Jandakova E, Turyna R, Langmeierova L, Weinberger V, Minar L
Title Evaluation of Cell-Free Urine microRNAs Expression for the Use in Diagnosis of Ovarian and Endometrial Cancers. A Pilot Study
Journal Pathology & Oncology Research. 2015.
Authors Luděk Záveský, Eva Jandáková, Radovan Turyna, Lucie Langmeierová, Vít Weinberger, Lenka Záveská Drábková, Martina Hůlková, Aleš Hořínek, Daniela Dušková, Jaroslav Feyereisl, Luboš Minář, Milada Kohoutová
Title Potential Urinary miRNA Biomarker Candidates for the Accurate Detection of Prostate Cancer among Benign Prostatic Hyperplasia Patients
Journal Journal of Cancer. 2014.
Authors Taha A Haj-Ahmad, Moemen AK Abdalla, Yousef Haj-Ahmad
Title Potential Urinary Protein Biomarker Candidates for the Accurate Detection of Prostate Cancer among Benign Prostatic Hyperplasia Patients
Journal Journal of Cancer. 2014.
Authors Taha A Haj-Ahmad, Moemen AK Abdalla, Yousef Haj-Ahmad

 


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