Rapid purification of total RNA from 5 mL - 10 mL urine without phenol

  • Isolate high quality total RNA and all sizes of circulating and exosomal RNA, including microRNA
  • No phenol extractions
  • Very sensitive and linear without the need for carrier RNA
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Isolate inhibitor-free urinary RNA
  • Slurry/Spin column and Slurry/96-well format available

ItemsCat. #Size
Urine Total RNA Purification Maxi Kit (Slurry Format) 29600 Variable
Urine Total RNA Purification Maxi 96-Well Kit (Slurry Format) 29650 1 x 96-well plate

Urine Total RNA Purification Maxi Kit (Slurry Format)

Norgen’s Urine Total RNA Purification Maxi Kit (Slurry Format) provides a rapid method for the isolation and purification of total RNA from 5 – 10 mL of urine. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies showed that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease specific outcomes, and prediction of therapeutic responses.

Kit Specifications
Minimum Urine Input
5 mL
Maximum Urine Input
10 mL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 10 Purifications
< 30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.


Kit Components
Component Cat. 29600 (variable)
Slurry C3 24 mL
Lysis Buffer A 2 x 130 mL
Wash Solution A 38 mL
Elution Solution A 6 mL
Mini Filter Spin Columns 50
Collection Tubes 50
Elution Tubes (1.7 mL) 50
Product Insert 1
Component Cat. 29650 (96-well)
Slurry C3 2 x 24 mL
Lysis Buffer A 4 x 130 mL
Wash Solution A 2 x 20 mL
Elution Solution A 2 x 6 mL
96-Well Filter Plate 1
Adhesive Tape 1
96-Well Collection Plate 1
96-Well Elution Plate 1
Product Insert 1

Title Total Extracellular Small RNA Profiles from Plasma, Saliva, and Urine of Healthy Subjects
Journal Scientific Reports. 2017.
Authors Yeri A, Courtright A, Reiman R, Carlson E, Beecroft T, Janss A, Siniard A, Richholt R, Balak C, Rozowsky J, Kitchen R, Hutchins E, Winarta J, McCoy R, Anastasi M, Kim S, Huentelman M, Van Keuren-Jensen K.
Title Supernatant versus exosomal urinary microRNAs. Two fractions with different outcomes in gynaecological cancers.
Journal Neoplasma. 2015.
Authors Zavesky L, Jandakova E, Turyna R, Langmeierova L, Weinberger V, Minar L
Title Evaluation of Cell-Free Urine microRNAs Expression for the Use in Diagnosis of Ovarian and Endometrial Cancers. A Pilot Study
Journal Pathology & Oncology Research. 2015.
Authors Luděk Záveský, Eva Jandáková, Radovan Turyna, Lucie Langmeierová, Vít Weinberger, Lenka Záveská Drábková, Martina Hůlková, Aleš Hořínek, Daniela Dušková, Jaroslav Feyereisl, Luboš Minář, Milada Kohoutová
Title Potential Urinary miRNA Biomarker Candidates for the Accurate Detection of Prostate Cancer among Benign Prostatic Hyperplasia Patients
Journal Journal of Cancer. 2014.
Authors Taha A Haj-Ahmad, Moemen AK Abdalla, Yousef Haj-Ahmad
Title Potential Urinary Protein Biomarker Candidates for the Accurate Detection of Prostate Cancer among Benign Prostatic Hyperplasia Patients
Journal Journal of Cancer. 2014.
Authors Taha A Haj-Ahmad, Moemen AK Abdalla, Yousef Haj-Ahmad