Urine Total RNA Purification Maxi Kit (Slurry Format)
Rapid purification of total RNA from 5 mL - 10 mL urine without phenol
Urine Total RNA Purification Maxi Kit (Slurry Format)
Rapid purification of total RNA from 5 mL - 10 mL urine without phenol
Overview
- Isolate high quality total RNA and all sizes of circulating and exosomal RNA, including microRNA
- No phenol extractions
- Very sensitive and linear without the need for carrier RNA
- Bind and elute all RNA irrespective of size or GC content, without bias
- Isolate inhibitor-free urinary RNA
- Slurry/Spin column and Slurry/96-well format available resin separation matrix
- Purification is based on Norgen’s proprietary resin matrix
Norgen’s Urine Total RNA Purification Maxi Kit (Slurry Format) provides a rapid method for the isolation and purification of total RNA from 5 – 10 mL of urine. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies showed that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease specific outcomes, and prediction of therapeutic responses.
Details
Supporting Data
Kit Specifications
|
|
Volume of Urine Processed |
5 - 10 mL
|
Size of RNA Purified |
All sizes, including < 200 nt
|
Time to Complete 10 Purifications |
< 30 minutes
|
Average Yield |
Variable depending on specimen
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 29600 (variable) | Cat. 29650 (96 preps) |
---|---|---|
Slurry C3 | 20 mL | 2 x 24 mL |
Lysis Buffer A | 2 x 130 mL | 4 x 130 mL |
Wash Solution A | 38 mL | 2 x 20 mL |
Elution Solution A | 6 mL | 2 x 6 mL |
96-Well Filter Plate | - | 1 |
Adhesive Tape | - | 1 |
Mini Filter Spin Columns | 50 | - |
Collection Tubes | 50 | - |
96-Well Collection Plate | - | 1 |
Elution Tubes (1.7 mL) | 50 | - |
96-Well Elution Plate | - | 1 |
Product Insert | 1 | 1 |
Documentation
FAQs
Maxi Slurry, High Throughput Maxi Slurry
RPM= √RCF/(1.118x10-5)(r)
Where RCF = required gravitational acceleration (relative centrifugal force in units of g); r = radius of the rotor in cm; and RPM = the number of revolutions per minute required to achieve the necessary g-force.
We recommend the following steps to prepare frozen urine for isolation:
- Gently warm the sample to room temperature or 37°C for 5 min.
- DO NOT perform a centrifugation step after thawing frozen cell-free Urine samples - this will eliminate the precipitated proteins leading to loss of protein-bound cf-NA or exosomes.
- Proceed with the protocol.
We recommend the use of Norgen’s Urine Preservative when collecting urine samples. Norgen’s Urine Preservative is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures, therefore no protein precipitation will occur and the purified nucleic acids will be of a higher quality.
Citations
Title | Clinical Significance of Rab27a as a Urinary Biomarker in Patients With Bladder Cancer |
Journal | KJUO. 2022. |
Authors | Ja Yoon Ku, Eu Chang Hwang, Chan Ho Lee, Kyung Hwan Kim, Dong Deuk Kwon, Hong Koo Ha |
Title | Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes. |
Journal | Scientific Data. 2021. |
Authors | Hutchins, E., Reiman, R., Winarta, J. et al. |
Title | Total Extracellular Small RNA Profiles from Plasma, Saliva, and Urine of Healthy Subjects |
Journal | Scientific Reports. 2017. |
Authors | Yeri A, Courtright A, Reiman R, Carlson E, Beecroft T, Janss A, Siniard A, Richholt R, Balak C, Rozowsky J, Kitchen R, Hutchins E, Winarta J, McCoy R, Anastasi M, Kim S, Huentelman M, Van Keuren-Jensen K. |
Title | Supernatant versus exosomal urinary microRNAs. Two fractions with different outcomes in gynaecological cancers. |
Journal | Neoplasma. 2015. |
Authors | Zavesky L, Jandakova E, Turyna R, Langmeierova L, Weinberger V, Minar L |
Title | Evaluation of Cell-Free Urine microRNAs Expression for the Use in Diagnosis of Ovarian and Endometrial Cancers. A Pilot Study |
Journal | Pathology & Oncology Research. 2015. |
Authors | Luděk Záveský, Eva Jandáková, Radovan Turyna, Lucie Langmeierová, Vít Weinberger, Lenka Záveská Drábková, Martina Hůlková, Aleš Hořínek, Daniela Dušková, Jaroslav Feyereisl, Luboš Minář, Milada Kohoutová |
Title | Potential Urinary miRNA Biomarker Candidates for the Accurate Detection of Prostate Cancer among Benign Prostatic Hyperplasia Patients |
Journal | Journal of Cancer. 2014. |
Authors | Taha A Haj-Ahmad, Moemen AK Abdalla, Yousef Haj-Ahmad |
Title | Potential Urinary Protein Biomarker Candidates for the Accurate Detection of Prostate Cancer among Benign Prostatic Hyperplasia Patients |
Journal | Journal of Cancer. 2014. |
Authors | Taha A Haj-Ahmad, Moemen AK Abdalla, Yousef Haj-Ahmad |