Name: Vibrio cholerae TaqMan PCR Kit Dx
Brand: Norgen Biotek Corp.
SKU: DxTM38500
Price: 325.000000 USD
Availability: InStock

Overview

Detection kit for Vibrio cholerae
CE-IVDD marked in accordance with the European Commission Directive 98/79/EC.
Research version available
Available in TaqMan format for analysis

Vibrio cholerae is a comma-shaped, gram-negative bacterium. It is the cause of cholera in humans, which affects the upper small intestine. Transmission of the disease is mainly through contaminated food or water. Human subjects affected by cholera exhibit severe watery diarrhea and vomiting, caused by the cholera toxin produced by the bacterium. Many cases of cholera are life-threatening, as diarrhea and associated vomiting can lead to rapid dehydration and electrolyte loss. Even with the extensive research on its epidemiology, cholera still affects over 5 million people per year worldwide.

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Vibrio cholerae TaqMan PCR Kit, 24 reactions

Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents

Details

Supporting Data

TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.

Storage Conditions

The Vibrio cholerae TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. DxTM38500 (24 rxns)
MDx TaqMan 2X PCR Master Mix Dx	550 µL
Vibrio cholerae Primer & Probe Mix Dx	2 x 70 µL
Vibrio cholerae Positive Control Dx - 200,000 copies/μL	50 µL
Nuclease-Free Water	2 x 1.25 mL
Product Insert	1

Vibrio cholerae TaqMan PCR Detection Kit Dx