• Detection kits for the Vibrio cholerae
  • Available in 3 formats for analysis:
 

ItemsCat. #Size
Vibrio cholerae TaqMan PCR Kit TM38500 24 rxns
Vibrio cholerae TaqMan Probe/Primer and Control Set TM38510 100 rxns
Vibrio cholerae SYBR Green PCR Kit SG38500 24 rxns
Vibrio cholerae End-Point PCR Kit EP38500 24 rxns
Vibrio cholerae Primer and Control Set EP38510 100 rxns

Vibrio cholerae Detection Kits

Vibrio cholerae is a comma-shaped, gram-negative bacterium. It is the cause of cholera in humans, which affects the upper small intestine. Transmission of the disease is mainly through contaminated food or water. Human subjects affected by cholera exhibit severe watery diarrhea and vomiting, caused by the cholera toxin produced by the bacterium. Many cases of cholera are life-threatening, as diarrhea and associated vomiting can lead to rapid dehydration and electrolyte loss. Even with the extensive research on its epidemiology, cholera still affects over 5 million people per year worldwide.

There are 3 kits available for the detection of Vibrio cholerae:

Vibrio cholerae TaqMan PCR Kit
  • PCR control to monitor for PCR inhibition and validate the quality
  • Master Mix for the target and PCR control detection
  • Primer and Probe mix
  • Positive control and a negative control to confirm the integrity of the kit reagents
Vibrio cholerae SYBR Green PCR Kit
  • Master Mix for the target detection
  • Control Master Mix for the PCR validation
  • Primers for the specific amplification of a region of the Vibrio cholerae genome
  • Positive control and a negative control to confirm the integrity of the kit reagents
Vibrio cholerae End-Point PCR Kit
  • Master Mix and primers for the specific amplification of a region of the Vibrio cholerae genome
  • Positive control and a negative control to confirm the integrity of the kit reagents
  • Loading dye and a DNA ladder to facilitate analysis of the results

For research use only and NOT intended for in vitro diagnostics.

Storage Conditions and Product Stability
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20°C without showing any reduction in performance.


Kit Components
Component TaqMan Probe
Cat. TM38500
(24 rxns)
SYBR Green
Cat. SG38500
(24 rxns)
End-Point
Cat. EP38500
(24 rxns)
MDx 2X PCR Master Mix - - 350 µL
MDx SYBR 2X PCR Master Mix - 350 µL -
MDx SYBR 2X PCR Control Master Mix - 350 µL -
MDx TaqMan 2X PCR Master Mix 350 µL - -
Vibrio cholerae Primer Mix 70 µL 70 µL 70 µL
Vibrio cholerae Positive Control 50 µL 50 µL 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL 1.25 mL
Loading Dye - - 100 µL
DNA Ladder - - 100 µL
Product Insert 1 1 1
Component TaqMan
Probe/Primer
and Control Set
Cat. TM38510
(100 rxns)
Primer
and Control Set
Cat. EP38510
(100 rxns)
Vibrio cholerae Primer Mix 3 x 70 µL 3 x 70 µL
Vibrio cholerae Positive Control 3 x 50 µL 3 x 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Product Insert 1 1