The 16S ribosomal RNA gene (16S rRNA) is a common target for microbial community classification in metagenomic studies. The gene is approximately 1,500 bp long and contains nine variable regions interspersed between conserved regions. These variable regions are frequently used in phylogenetic classifications such as genus or species in diverse microbial populations. The 16S region to sequence is an area of debate, and your region of interest might vary depending on multiple factors including experimental objectives, design, and sample type.
Norgen offers optimized 16S and ITS sequencing library preparation kits that accommodate a range of sample types and input quantities. Our library prep kits provide end-users with a streamlined workflow experience that consistently yield high-quality NGS libraries across a range of sample types. With Norgen offering NGS library preparation kits for nine 16S regions, two ITS regions, and multiplex panels of both 16S/ITS, you can choose the kit that best suits your research needs. Our library preparation kits come in sizes to accommodate as few as 24 samples and as many as 384 uniquely-indexed samples that can be sequenced in a single NGS run.
|Name||24 Prep||96 Prep set A||96 Prep set B||96 Prep set C||96 Prep set D|
|16S V1-V2 Library Preparation Kit for Illumina||70100||70110||70120||70130||70140|
|16S V1-V3 Library Preparation Kit for Illumina||70200||70210||70220||70230||70240|
|16S V2-V3 Library Preparation Kit for Illumina||70300||70310||70320||70330||70340|
|16S V3-V4 Library Preparation Kit for Illumina||70400||70410||70420||70430||70440|
|16S V3-V5 Library Preparation Kit for Illumina||70500||70510||70520||70530||70540|
|16S V4 Library Preparation Kit for Illumina||70600||70610||70620||70630||70640|
|16S V4-V5 Library Preparation Kit for Illumina||70700||70710||70720||70730||70740|
|16S V5-V7 Library Preparation Kit for Illumina||70800||70810||70820||70830||70840|
|16S V7-V9 Library Preparation Kit for Illumina||70900||70910||70920||70930||70940|
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