16S V1-V3 Library Preparation Kit for Illumina The reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms For research use only and NOT intended for in vitro diagnostics. Powered by Bioz 16S V1-V3 Library Preparation Kit for Illumina The reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms SKU 70200 Size 24 Preps 96 Preps Set A 96 Preps Set B 96 Preps Set C 96 Preps Set D Contact Distributor Overview Details Documentation Citations Overview Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more Simple and quick workflow: library could be prepared in less than 5 hours Component of Norgen's metagenomics workflow A single NGS run can be prepared with up to 384 unique dual-index libraries Sample type purification kit guide The 16S V1-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others. The 16S V1-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5' and 3' barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing. Details Supporting Data Previous Figure 1. 16S V1-V3 PCR1 Amplification Figure 2: 16S V1-V3 Indexed Libraries Figure 3: BioAnalyzer Trace of the 16S V1-V3 Indexed Library Next Click for expanded view Minimum amount of starting material: 2.5 µL of DNA (5 ng/µL) Time to complete library preparation: 4 hours Storage Conditions and Product Stability Norgen’s 16S V1-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival. All kit components should remain stable for at least 1 year when stored at the specified storage conditions. Step Component Cat. 70200 (24 preps) Cat. 70210, 70220, 70230, 70240 (96 preps) Amplicon PCR (PCR 1) MGX Master Mix 330 µL 1,320 µL 16S V1-V3 Primer Mix 70 µL 280 µL Index PCR (PCR 2) Indexing Master Mix 660 µL 2 x 1,320 µL N7xx Index Primer 50 µL 50 µL S5xx Index Primer 70 µL 70 µL PCR Clean-Up Resuspension Buffer 2 x 1,250 µL 2 x 5,000 µL Nuclease-free water 1,250 µL 1 x 6,000 µL Documentation PROTOCOLS (70200,70210,70220,70230,70240) 16S V1-V3 Library Preparation Kit for Illumina - Protocol Citations Title Characterization of the urogenital microbiome in Miniature Schnauzers with and without calcium oxalate urolithiasis Journal Journal of Veterinary Internal Medicine. 2022. Authors Emily L. Coffey,Andres M. Gomez,Erin N. Burton,Jennifer L. Granick,Jody P. Lulich,Eva Furrow Related Services NGS Related Products Bacterial Genomic DNA Isolation Kit Biofilm DNA Isolation Kit Cells and Tissue DNA Isolation Kit Fecal DNA Collection & Preservation Mini Tubes Food DNA Isolation Kit Genomic DNA Isolation Kit Microbiome DNA Isolation Kit Olive Oil DNA Isolation Kit Milk Bacterial DNA Isolation Kit Plant RNA/DNA Purification Kit Plant/Fungi DNA Isolation Kit Stool DNA Isolation Kit Stool Nucleic Acid Collection and Preservation Tubes Synovial Fluid Bacterial Genomic DNA Isolation Kit Urine DNA Isolation Kit for Exfoliated Cells or Bacteria
16S V1-V3 Library Preparation Kit for Illumina The reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms For research use only and NOT intended for in vitro diagnostics. Powered by Bioz 16S V1-V3 Library Preparation Kit for Illumina The reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms SKU 70200 Size 24 Preps 96 Preps Set A 96 Preps Set B 96 Preps Set C 96 Preps Set D Contact Distributor
