16S V4 Library Preparation Kit for Illumina (Cat. 70600, 70610, 70620, 70630, 70640)
16S V4 Library Preparation Kit for Illumina
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16S V4 Library Preparation Kit for Illumina
The reagents and components required for library preparation of the 16S V4 amplicon libraries to be used for next-generation sequencing on Illumina platforms
- Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
- Simple and quick workflow: library could be prepared in less than 5 hours
- Component of Norgen's metagenomics workflow
- A single NGS run can be prepared with up to 384 unique dual-index libraries
For research use only and NOT intended for in vitro diagnostics.
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(Cat. 70600) 24 Preps
(Cat. 70610) 96 Preps Set A
(Cat. 70620) 96 Preps Set B
(Cat. 70630) 96 Preps Set C
(Cat. 70640) 96 Preps Set D
Product Size
Kit Specifications
| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V4 Library Prep Kit for Illumina is shipped as one kit box (for
the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be
stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V4 Library Prep Kit for Illumina is shipped as one kit box (for
the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be
stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V4 Library Prep Kit for Illumina is shipped as one kit box (for
the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be
stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V4 Library Prep Kit for Illumina is shipped as one kit box (for
the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be
stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V4 Library Prep Kit for Illumina is shipped as one kit box (for
the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be
stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
Supporting Data
| Title | Characterization of the urogenital microbiome in Miniature Schnauzers with and without calcium oxalate urolithiasis |
| Journal | Journal of Veterinary Internal Medicine. 2022. |
| Authors | Emily L. Coffey,Andres M. Gomez,Erin N. Burton,Jennifer L. Granick,Jody P. Lulich,Eva Furrow |
16S V4 Library Preparation Kit for Illumina
The 16S V4 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V4 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V4 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V4 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5' and 3' barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Supporting Data
Kit Specifications
| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V4 Library Prep Kit for Illumina is shipped as one kit box (for
the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be
stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V4 Library Prep Kit for Illumina is shipped as one kit box (for
the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be
stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V4 Library Prep Kit for Illumina is shipped as one kit box (for
the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be
stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V4 Library Prep Kit for Illumina is shipped as one kit box (for
the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be
stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V4 Library Prep Kit for Illumina is shipped as one kit box (for
the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be
stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Title | Characterization of the urogenital microbiome in Miniature Schnauzers with and without calcium oxalate urolithiasis |
| Journal | Journal of Veterinary Internal Medicine. 2022. |
| Authors | Emily L. Coffey,Andres M. Gomez,Erin N. Burton,Jennifer L. Granick,Jody P. Lulich,Eva Furrow |
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